Study on the UVB-Induced MMP-9 and TIMP-1 mRNA Expression in HaCaT Cells

目的:研究中波紫外线(UVB)照射对HaCaT细胞中基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制剂-1(TIMP-1)mRNA表达的影响.方法:培养HaCaT细胞,绘制细胞生长曲线,经不同剂量(0、30、60、90 mJ/cm2) UVB照射;用MTT方法测定UYB照射后细胞的增殖活性,反转录-聚合酶链反应(RT-PCR)方法测定UVB照射后HaCaT细胞中MMP-9 mRNA和TIMP-1 mRNA的表达.结果:随着照光剂量的增大,细胞的增殖活性逐渐降低,而细胞的增殖抑制率逐渐增加,不同照射剂量各组间光密度(OD)值差异有统计学意义(P＜0.05);随着照光剂量的增大,MMP-9mRNA的表达逐渐增加,各组之间比较差异均有统计学意义(P＜0.05);TIMP- 1mRNA的表达逐渐降低,与0mJ/cm2比较差异有统计学意义(P＜0.05).结论:UVB照射可诱导HaCaT细胞损伤和细胞凋亡,MMP-9和TIMP-1可能与光老化的发生有一定关系.     

国人不同剂量布比卡因复合芬太尼腰麻在肛肠疾病患者日间手术中的效果比较

目的 探索最佳适合国人的肛肠手术患者日间手术的腰麻用药方案.方法 ASA Ⅰ～Ⅱ级肛肠手术患者80例,随机分成两组,按照腰麻用药方案不同分别为0.75％布比卡因0.3 ml+芬太尼15 μg+50％葡萄糖0.05 ml(A组)和0.75％布比卡因0.5 ml+芬太尼15 μg+50％葡萄糖0.05 ml(B组),记录两组患者围术期血流动力学变化和麻醉后患者感觉,运动阻滞起效和持续时间,肛门松弛度,手术后恶心、呕吐、尿潴留、瘙痒等不良反应,外科医师和患者满意率.结果 B组术前与术后血流动力学明显变化,A组无明显变化.B组感觉阻滞起效时间短于A组,持续时间明显较A组长.运动阻滞强度B组大于A组,肛门松弛度A组差于B组.患者满意度和外科医师满意度B组较A组高.手术后恶心、呕吐、尿潴留、瘙痒发生率无明显差异.B组低血压发生率较A组高.结论 B组0.75％布比卡因0.5 ml+芬太尼15 μg是国人腰麻进行肛肠日间手术较为适合的麻醉用药剂量.     

Gefitinib-induced disseminated intravascular coagulation in a patient with non-small cell lung cancer

In February 2005, Gefitinib （Iressa）, a small-molecular .epidermal growth factor receptor and tyrosine kinaseinhibitor, was approved in China as an anticancer agent for patients with advanced （local or metastatic） non-small cell lung cancer （NSCLC）, who failed prior chemotherapy. The common adverse events of the drug include acne-like skin rash, paronychia, pruritus, diarrhea, nausea/vomiting, anorexia, hepatitis, and hyperbilirubinemia.1 However, these adverse events are generally mild in severity and reversible on cessation of the treatment. Therefore, gefitinib has been regarded as a relatively safe agent,     

手术加放疗治疗耳部瘢痕疙瘩的疗效分析

目的研究手术加术后放疗对耳部瘢痕疙瘩的治疗效果。方法我科2005年12月～2011年1月收治耳部瘢痕疙瘩患者30例(39耳),全部采用手术切除加术后直线加速器6Mev电子线照射,隔日一次,共5次,每次放射剂量3Gy,总剂量15Gy。结果治疗结束后1年,39侧患耳中治愈32耳(82.1%),有效3耳(7.7%),总有效率为89.7%,有4耳复发,复发率10.3%;至目前已平均随访37.3月,有11耳复发,复发率28.2%,复发的平均发生时间为19.7月。结论手术切除加术后放疗对耳部瘢痕疙瘩的近期治疗效果令人满意,但其远期疗效不容乐观;对耳部瘢痕疙瘩的随访时间应至少在2年以上。     

防己黄芪汤对单肺通气患者的保护作用

目的:观察防己黄芪汤对开胸单肺通气患者术中肺再灌注损伤的保护作用.方法:46例食道癌择期开胸手术患者,分级属美国麻醉医师协会Ⅰ～Ⅱ级,随机分为2组,每组23例:A组为中药干预+常规治疗组,术前连续7d口服防己黄芪汤;B组为常规治疗对照组.术中及术后分别在麻醉诱导后双肺通气时(T1)、单肺通气后1h(T2)、单肺通气后2 h(T3)及术后2 h(T4)时段,采外周动脉血3 mL进行分析,测定患者动脉氧分压(PaO2)、动脉氧饱和度(SpO2)、血浆超氧化物歧酶( SOD)、丙二醛(MDA)及中性粒细胞(PMN)计数的数值.结果:A组PaO2和SpO2在T2,T3,T4时段较B组高,有显著差异(P＜0.01).麻醉后,两组血浆SOD均下降,MDA及PMN升高.在T1,T2,T3,T4时段,A组SOD下降的程度明显小于B组(P＜0.01);在T2,T3,T4时段,A组MDA升高的程度较小,与B组比较有显著差异(P＜0.01);在T1,T2,T3,T4时段,A组PMN升高的程度较小,与B组比较有显著差异(P＜0.01).结论:中药防己黄芪汤对单肺通气、肺缺血再灌注(LIRI)所致的肺损伤,有较好保护作用.     

士的宁对多巴胺能神经元的保护作用

目的:选择2种神经毒物1-甲基-4-苯基吡啶离子(MPP+)和谷氨酸在体外诱导多巴胺能神经元退行性损伤,并检测士的宁对此细胞的药理毒理作用,及对其退行性损伤的协同作用或拮抗作用。方法:采用孕14 d小鼠,取出胎鼠中脑,分离出多巴胺能神经元,调整细胞密度7.5×105/mL,于5%CO2,37℃,100%湿度条件培养,于体外培养的第10天分别加入士的宁0.1,1,5,10μmol.L-1,MPP+10μmol.L-1,谷氨酸500μmol.L-1作用48 h,第12天染色。选取细胞数,神经元树突长度和数量作为观察指标,并做出分析。结果:士的宁对未损伤的多巴胺能神经元在48 h培养过程中无毒性影响。在10μmol.L-1士的宁作用下,多巴胺能神经元数量明显高于对照组(P<0.05);在5～10μmol.L-1士的宁作用下,树突数量与对照组相比也有明显提高(P<0.05)。士的宁(10μmol.L-1)和MPP+共同培养48 h后,细胞计数明显高于MPP+单独培养组(P<0.05);这种保护作用更为明显的表现在细胞形态学变化方面,在10μmol.L-1的士的宁剂量下,神经树突长度明显高于MPP+单独培养组(P<0.05);神经树突的数量在5μmol.L-1士的宁作用下与MPP+单独培养组相比有明显增加(P<0.05)。在对谷氨酸(500μmol.L-1)诱导的神经元损伤模型中,与谷氨酸组相比,10μmol.L-1的士的宁对所有的观察指标的数值都有递增影响(P<0.05)。结论:首次证实士的宁具有可在体外刺激中脑分离出的多巴胺能神经元的生长,并且对此神经元退行性损伤有保护作用。     

运用灰色关联度模型分析乙脑发病与气象要素、运气理论相关性

乙脑是一种严重危害人类健康的急性传染病.文章利用1951～1990年安徽省乙脑发病资料和气象资料,通过建立灰色关联度模型进行计算与分析,探讨乙脑发病与气象要素的相关性,验证运气理论关于乙脑发病相关解释的合理性,并为乙脑预测预防提供参考依据.     

Immunohistochemical expression of cell-cycle regulators in pediatric embryonal brain tumors

Embryonal tumors constitute the most common malignant brain tumor group in children. Experimental results indicate that genes involved in cell cycle and signal transduction are deregulated in medulloblastoma (MB) and atypical teratoid/rhabdoid tumors (AT/RT). The cell cycle is regulated by protein complexes composed of a regulatory subunit called Cyclin and a catalytic domain named Cyclin-dependent kinase (CDK). Cyclins and CDKs are in turn regulated by cyclin-dependent kinase inhibitors (CDKI) which inhibit cell-cycle progression. Cyclins D and Cyclin E are important for the passage of cells through G1 to S phase. P-27, a member of the universal CDKI family, is important in regulating the G1/S transition. Thus, the purpose of this study was to investigate the expression of p-27, Cyclin D3, and Cyclin E, and their possible prognostic significance in pediatric embryonal brain tumors. We retrospectively evaluated 51 children with embryonal tumors that were treated surgically in our institute. All patients had regular follow up examinations. The streptavidin-biotin HRP method was performed on paraffin sections for detection of p-27, Cyclin D3, and Cyclin E. There were 42 cases of MB and nine cases of AT/RT. Cyclin D3 expression was detected in 11/42?MB and 3/9 AT/RT patients. Cyclin E expression was detected in 28/42?MB and 8/9 AT/RT patients. High expression of Ki-67 (>50?%) and p-27 (>50?%) was observed in 23.8-73.8?% of MB patients. Combined high Ki-67 and p-27 expression was observed in 21.4?% cases of MB. In these cases there was expression of Cyclin E in 88.8?% and Cyclin D3 in 22.2?% of MB. No significant correlation was found between Ki-67 and p-27, Cyclin D3, and E. No correlation was found between Cyclin D3, Cyclin E, p-27, and overall survival. Increased p-27 and Cyclin E expression was detected in a substantial number of MB patients and in nearly all AT/RT patients. Further studies on a larger number of patients are needed to clarify a possible correlation of p-27 and Cyclin E with tumor behavior.