新疆和田维吾尔族自然长寿老人血管紧张素Ⅱ、醛固酮及生化代谢等指标的检测与分析

目的探讨新疆和田地区维吾尔族长寿老人血浆血管紧张素Ⅱ、醛固酮及生化代谢等指标的变化。方法随机抽取100例自然长寿老人(≥90岁)(长寿组)和106例(65～70岁)老年人(老年组),测定血浆血管紧张素Ⅱ、醛固酮、血脂、血糖、胰岛素、血肌酐、尿肌酐、血尿酸及尿液电解质水平。结果 (1)长寿组的血管紧张素Ⅱ高于老年组,而醛固酮则低于老年组,两组间比较差异均有统计学意义(P<0.05)。(2)长寿组空腹血糖、血胰岛素、胰岛素抵抗指数、甘油三酯均低于老年组,而长寿组总胆固醇、高密度脂蛋白胆固醇均高于老年组,两组比较除空腹血糖外均有显著性差异统计学意义(P<0.05),长寿组和老年组血肌酐未见明显差异(P>0.05),但长寿组老人尿肌酐和血尿酸均高于老年组(P<0.05)。(3)长寿组的尿钾高于老年组(P<0.05),尿比重低于老年组(P<0.05),两组尿钠和尿pH未见明显差异(P>0.05)。结论血管紧张素Ⅱ增高、醛固酮降低的分离现象,总胆固醇、高密度脂蛋白胆固醇相对增高及尿钾水平相对较高是该地区长寿老人的特征。     

MiR-361-5p inhibits colorectal and gastric cancer growth and metastasis by targeting staphylococcal nuclease domain containing-1

MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, we investigated the biological role and mechanism of miR-361-5p in colorectal carcinoma (CRC) and gastric cancer (GC). We showed that microRNA-361-5p (miR-361-5p) was down-regulated in CRC and GC in comparison to the controls. Meanwhile, the expression levels of miR-361-5p negatively correlated with lung metastasis and prognosis in clinical CRC patients. Overexpression of miR-361-5p markedly suppressed proliferation, migration and invasion of cancer cells. Additionally, this phenotype could be partially rescued by the ectopic expression of staphylococcal nuclease domain containing-1 (SND1). SND1 was identified as a target of miR-361-5p using bioinformatics analysis and in vitro luciferase reporter assays. In turn, SND1 bound to pre-miR-361-5p and suppressed the expression of miR-361-5p, thus exerting a feedback loop. Most interestingly, in vivo studies showed that restoration of miR-361-5p significantly inhibited tumor growth and especially the lung metastasis in nude mice. Therefore, it could be concluded that miR-361-5p functions as a tumor-suppressive miRNA through directly binding to SND1, highlighting its potential as a novel agent for the treatment of patients with CRC and GC.     

丙泊酚复合瑞芬太尼全凭静脉或地氟醚平衡麻醉对听神经瘤手术后苏醒的影响

目的 观察丙泊酚复合瑞芬太尼全凭静脉或地氟醚平衡麻醉对听神经瘤手术后苏醒的影响。方法 40例ASAⅠ~Ⅱ级择期行乙状窦入路或颅中窝入路听神经瘤手术的成年患者,随机分为丙泊酚复合瑞芬太尼组(PR组,n=20) 和地氟醚平衡麻醉组(DR组,n=20)。记录患者一般情况;记录唤醒时间、拔除气管导管时间和定向力恢复时间;记录2组患者手术结束时的麻醉深度、肌肉松弛恢复情况、拔管时心率和血压变化;记录术后不良事件。结果 PR组唤醒时间早于DR组2.4 min (P=0.033);PR组拔除气管导管时间短于DR组4.9 min (P=0.002);PR组定向力恢复时间短于DR组23.7 min(P=0.049);PR组拔除气管导管时血压和心率变化均小于DR组(P<0.05)。结论 丙泊酚复合瑞芬太尼全凭静脉麻醉用于听神经瘤手术利于患者术后快速苏醒,地氟醚组患者拔管时心率和血压变化较明显。     

新疆地区ⅠB～ⅡA期宫颈癌不同治疗模式的疗效及并发症分析

目的 探讨新疆地区ⅠB～ⅡA期宫颈癌不同治疗模式的疗效及并发症。方法 回顾性分析本院215例ⅠB～ⅡA期宫颈癌患者的临床病理资料并随访其生存情况,分析总体及不同临床病理参数的5年生存率,采用Cox模型分析影响预后的独立因素;根据治疗方案将215例患者分为根治性手术（81例）、根治性放疗（65例）、术前辅助治疗（25例）及术后辅助治疗（44例）,分析各种治疗模式的预后及并发症情况。结果 总体5年生存率为79.3％。不同FIGO分期、族别、肿瘤直径、分化程度及术后病理高危因素的5年生存率差异均有统计学意义（P＜0.05）;Cox模型分析显示,FIGO分期、肿瘤直径及术后病理高危因素是宫颈癌预后的独立危险因素（P＜0.05）。根治性手术、根治性放疗、术前辅助治疗及术后辅助治疗者的5年生存率依次为80.9％、82.5％、78.8％和72.4％,并发症发生率依次为25.9％、18.5％、24.0％和38.6％,差异均无统计学意义（P＞0.05）。结论 对术后有危险因素或术前肿瘤体积较大患者行相应辅助治疗可达到无危险因素患者根治性手术或根治性放疗的治疗效果,故建议临床工作中对不同患者施行个体化治疗,在保证治疗效果的前提下尽量减少并发症及患者治疗负担。     

异常粘液质与心血管病的关系及其防治探讨

根据维吾尔医药理论，我们认为异常粘液质与心血管病可能有一定的关系。维吾尔医认为正常粘液质体液属性湿赛，可补充人体所需要的液体，其湿性及成分中营养物在自身作用范围内不仅可作为全身需要的营养，还可防止类似于胆液质的热性物质对其它体液进行分解破坏，预防人体出现异常变化。心包、胸腔、关节腔内的粘液质可减缓磨擦刺激。当人体营养不足或血液等过量流失时，可替代血液；可增加血液汁，帮助血液对器官的湿化作用。粘液质体液在把随自己一同流动的胆液质、血液质、黑胆质中的主要营养物输送至人体最微小部位，并携带该部位物质代谢过程产生的废物流回血液，通过相应的途经排出体外的过程中，起到了非常重要的作用。     

MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1

In this study, we investigated the mechanism of miR-200c-3p and SLC6A1 in regulating cell activity of clear cell renal cell carcinoma (CCRCC). The mRNA and miRNA expressions of tissue specimens were analyzed by CapitalBio Corporation (Beijing, China). The expression of SLC6A1 in CCRCC cells was examined through qRT-PCR and western blot. The migration and invasion ability of 786-O cells was testified by transwell assay after transfected. 786-O cell proliferation ability was detected by MTT assay. Dual luciferase reporter assay verified the association between SLC6A1 and miR-200c-3p. SLC6A1 was high expressed and miR-200c-3p was low expressed in CCRCC tissues and cells. Besides, lower SLC6A1 expression indicated longer survival time and higher survival rate. MiR-200c-3p could directly target at SLC6A1 and reduce its expression. MiR-200c-3p inhibited the proliferation, migration and invasion in 786-O cells by down-regulating SLC6A1 expression. The results suggested that the miR-200c-3p served as a suppressor for CCRCC via down-regulating SLC6A1.     

Tracking Long-Term Survival of Intramyocardially Delivered Human Adipose Tissue-Derived Stem Cells Using Bioluminescence Imaging

Purpose  

Transplantation of a regenerative cell population derived from human subcutaneous adipose tissue (hASCs) for cardiac regeneration represents a promising therapy due to the capacity of these cells for proliferation and differentiation. Understanding the fate of injected hASCs would help to understand how hASCs work in vivo. The aim of this study was to track the long-term fate, including survival, differentiation, proliferation, apoptosis, migration, and growth factor secretion of intramyocardially injected hASCs following experimental acute myocardial infarction in an immunodeficient mouse model.     

新疆地区耳鼻咽喉头颈外科学硕士学位论文分析及建言

目的:了解新疆地区耳鼻咽喉头颈外科学硕士研究生的知识结构、能力的基本状况及其发展趋势,为进一步提高硕士研究生的培养水平提供参考依据.方法:抽取1998-2009年新疆地区耳鼻咽喉头颈外科硕士学位论文46篇,就论文类型、选题范围、指标选择、统计方法等方面进行分析,探讨其优势与特色,针对其不足提出对策.结果:在46篇硕士学位论文中,医学科学学位论文9篇(19.57%),临床专业学位论文37篇(80.43%);实验研究论文5篇,临床研究论文30篇,有临床与实验研究结合论文10篇,实验流行病学研究论文1篇;研究的病种覆盖耳科、鼻科、咽喉科、头颈肿瘤、睡眠呼吸暂停综合征及气管支气管等耳鼻咽喉头颈外科学各领域;软件包、统计描述等各种统计方法均有涉及;引用参考文献平均37.46篇,其中外文文献19.55篇,引用的近5年的文献平均13.57篇;硕士研究生由4个民族组成,硕士研究生导师具有专业高级职称.结论:学位论文以临床研究为重点,突出多发病和热点领域研究,应用先进研究方法,充分参考国内外最新文献.基础研究需创新,重点突出学科特色,避免临床研究过多重复.