谨防网络性心理障碍

随着电脑的普及，电脑已渗透到社会的方方面面。痴迷电脑比比皆是，伴随电脑而生的网络性心理障碍已受到了心理学家、医学家的广泛关注。     

先天性聋儿伴有孤独症倾向的分析与护理干预

目的了解先天性聋儿伴有孤独症倾向的发生率并进行护理干预。方法采用儿童期孤独症评定量表（CARS）并结合美国精神障碍诊断和统计手册（DSM-IV）儿童孤独症的诊断标准,对51例先天性聋儿及44例正常儿童进行对照性测评,并对患有孤独症的聋儿进行护理干预,1个月后进行量表评定。结果本组先天性聋儿孤独症发生率为57%。2组患者CARS评分比较有显著性差异（P〈0.05或P〈0.001）。先天性聋儿孤独症护理干预前后CARS评分显示除对玩具等物品的使用和非言语沟通外,其余5项差异有统计学意义（P〈0.05或P〈0.001）。结论超过50%的先天性聋儿患者合并孤独症,护理干预能明显改善先天性聋儿的孤独症状。     

天津市河西区儿童多动症患病率调查

儿童多动症又名轻微脑功能失调(Minim-al Brain Dysfunction,M.B.D),也有称注意缺陷障碍(Attention eficit Disorders,A.D.D)。国内外文献报道患病率1.5～10.0%之间,男女比例为:4～9:1。本病不仅严重影响儿童的学习、生活和身心健康,而且可能是成人精神病的前身,有的可能沦落为少年犯,已引起家长、学校及社会各界的广泛关注,它也是儿童精神病学、儿科学、心理学以及教育学共同关心的问题之一。我们     

输尿管镜钬激光碎石术22例护理配合

2008年1月～2009年10月,我们对22例尿路结石患者行输尿管镜钬激光碎石术,并给予密切护理配合,效果满意.现将护理配合体会报告如下. 1资料与方法 1.1临床资料 本组22例,男14例,女8例;年龄30～75岁,平均57岁.其中输尿管结石16例,膀胱结石6例;结石大小(0.3～1.4)cm×(0.5～3.5) cm.均行输尿管镜钬激光碎石术,均碎石成功.     

MEBO治疗阑尾炎切口感染7例分析

作者根据徐荣祥教授提出的“烧伤是一切创伤及溃疡的总和”的理论,应用MEBO治疗阑尾炎术后切口感染7例,认为有活血化瘀、消炎止痛、祛腐生肌的功能,促进坏死组织液化,加快切口愈合,缩短疗程,效果满意。     

血友病的临床治疗：第55届美国血液学会年会报道

血友病患者的预后在过去50年中有了显著提高.随着目前越来越多安全的凝血因子浓缩物的出现,血友病临床治疗的主要焦点成为预防长期并发症,尤其是在重型血友病患者中应预防可致残的关节病.对于有少量出血史、重型血友病A的幼年患者,早期预防性凝血因子输注治疗可取得确切的临床疗效.对于已存在关节病的青少年和成年血友病患者,可从预防性治疗中获益.凝血Ⅷ因子抑制物的产生是凝血因子替代治疗最棘手的并发症,临床上可采用抑制物免疫耐受诱导疗法来克服抑制物的作用.文章介绍了2013年第55届美国血液学会（ASH）年会关于临床治疗儿童及成年人血友病的研究进展.     

微囊化成人胰岛细胞治疗大鼠糖尿病

1 临床资料成人胰岛采用传统的胰腺导管内注射胶原酶消化方法,应用新型胶原酶-Liberase酶分离胰岛,Ficoll纯化胰岛. 用静电微囊发生器进行海藻酸钠-多聚赖氨酸-海藻酸钠(APA)微囊成囊,并用APA微囊包裹成人胰岛细胞. 健康SD大鼠按65 mg/kg剂量肌肉注射链脲菌素(美国Sigma公司),以尿糖强阳性、非禁食血糖>16.5 mmol/L且病程>14 d者作为糖尿病动物模型. 将合格糖尿病大鼠模型27只随机分成3组(空囊组、游离胰岛组、微囊组),每组各9只,以体质量及血糖作为观察指标.     

白三烯受体拮抗剂安可来治疗哮喘的临床研究

全部轻、中度哮喘病人均按需吸入沙丁胺醇（喘乐宁）,其余如吸入肾上腺皮质激素、茶碱等在整个实验期间不得变更种类及剂量。将病人随机分为安可来组３８例,给予安可来 ４０ ｍｇ／ｄ,早、晚各服 ２０ ｍｇ,曲尼司特组 ２７例,予曲尼司特３００ ｍｇ／ｄ,早、晚各服１５０ｍｇ。 结果：安可来组服药前、后第一秒用力呼气量（ＦＥＶ１）升幅８．４％,曲尼司特组升幅６．５％;安可来组在夜间憋醒次数和肺部体征方面少于曲尼司特组,但尚无显著性差异。临床综合评价,安可来组有效率为７６％,高于曲尼司特组的５６％。安可来组有１例…     

异基因外周血造血干细胞移植治疗慢性髓细胞白血病的临床研究

为了评价异基因外周血造血干细胞移植 (allo PBSCT)治疗慢性髓细胞白血病 (CML)的疗效 ,作者对 12例CML进行了allo PBSCT的临床研究 ,12例均为第 1次慢性期 ,中位年龄 32 .5岁 ;全部供受者HLA配型完全相合 ,供者动员用G CSF 5 μg/ (kg·d)× 5天 ,分离外周血单个核细胞 (2 .49.5 7)× 10 8/kg(中位数 5 .0× 10 8/kg) ,CD 34 + 细胞数为 (0 .72 6 6 .30 )× 10 6/kg(中位数 5 .82× 10 6/kg)。预处理方案 :9例采用全身照射 (TBI) +环磷酰胺 (CTX) +依托泊甙 (VP16 ) ,3例采用TBI +CTX ;移植物抗宿主病 (GVHD)预防采用环孢素A(CsA) +短程甲氨喋呤 (MTX)方案。全部患者均顺利造血重建 ,中性粒细胞 >0 .5× 10 9/L中位数 14.5天 (142 3天 ) ,血小板 >2 0× 10 9/L中位数 16天 ;发生Ⅱ度以上急性GVHD 3例 (2 5 % ) ;慢性GVHD共 5例 ,其中局限性 3例 ,广泛性 2例 ;死亡 3例 ,2例死于感染 ,1例死于间质性肺炎 ,中位随访 6个月 ,存活 9例。Allo PBSCT急性GVHD发生率并不比异基因骨髓移植高 ,但慢性GVHD的发生应引起重视     

Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway

Objective:To study the immunologic function of dendritic cells(DCs) cultured in two kinds of hepatoma cell line’s supernatant and the enhancing effects of carboxymethylpachymaran(CMP) on DCs.Methods:DCs were harvested after stimulation by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin(IL)-4 from umbilical cord blood using density-gradient centrifugation method. Cultured supernatant of two hepatoma cell lines(HepG2 and HepG2.2.15) were collected for condition medium(CM) according to a volume ratio of supernatant to incomplete RPMI-1640 medium,which was 3:1.CMP was dissolved in incomplete RPMI-1640 medium.Experimental groups were divided according to the culture medium,either CM or with CMP in it.DCs subsets CD83,CD86,CD1a,and d-related human leukocyte antigens(HLA-DR) were analyzed by flow cytometry.The proliferation ability of allogeneic T cells in mixed lymphocyte reaction(MLR) stimulated by DCs was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) analysis.IL-12p70,interferon-γ(IFN-γ),and nuclear factor k B(NF- k B) were detected by enzyme-linked immunosorbent assay analysis.Results:The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of CM were lower than those of normal group(P<0.01).Compared with the normal group,groups treated with CMP showed a higher expression level of DCs subsets,lymphocyte reproductive activity,as well as IL-12 and IFN-γsecretion levels.Groups treated with CMP also demonstrated higher levels of DCs phenotype expression and IL-12 and IFN-γsecretion in supernatant of MLR and higher lymphocyte reproductive activity compared with CM group(P<0.05).Compared with the normal group,the expression level of NF-k B in DCs nuclear was higher in CMP groups but lower in two CM groups(P<0.05).After CMP was added,the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added(P<0.05).However,there was no significant difference between the two CM groups(P>0.05).Conclusions:Two kinds of hepatoma cell line’s supernatant can inhibit the immunologic function of DCs.This suppressive effect may be related to the inhibition of NF-κB/Rel pathway.CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway.