高出检测限浓度的肿瘤标志物的准确值检测

目的为得到一个高出检测限浓度肿瘤标志物的具体值,而选择一种更好的稀释液对样本进行稀释,以满足临床对疗效观察的需求。方法分别采用三种不同的稀释剂：①美国雅培AXSYM全自动化学发光仪配套的第4号缓冲液;②生理盐水;③肿瘤标志物的阴性血清（每种被测肿瘤正常值低90%以下的血清）,对美国雅培AXSYM全自动化学发光仪所测的高浓度肿瘤标志物CEA,CA125,T-PSA,进行稀释,与仪器自动稀释的结果和在仪器检测限内检测结果进行比较。结果手工稀释的结果与机器自动稀释的结果比较,CA125：4号缓冲液稀释与仪器自动稀释结果无显著差异,而用生理盐水和用阴性血清稀释与仪器自动稀释有统计学差异;CEA和T-PSA：三种稀释液与仪器自动稀释均无显著性差异。结论美国雅培AXSYM全自动化学发光仪配套的4号缓冲液,对超高浓度即仪器已经不能自动稀释的那部分肿瘤标志物的血清稀释的结果最好。CA125阴性血清由于基础值高,则不能用来对CA125进行稀释,但可以对CEA和T-PSA进行稀释,但是结果不如4号缓冲液和生理盐水准确。     

负压封闭引流技术在头皮挫裂伤中的应用

头皮挫裂伤多见于交通事故,常伴有泥土等异物,即使及时清创,伤口感染风险高。负压封闭引流技术(vaccum sealing drainage,VSD)为处理复杂创口的有效办法。自2013年以来,我们应用VSD处理头皮挫伤6例,取得满意疗效。1资料与方法1.1一般资料6例中,男4例,女2例;年龄34~58岁。交通事故伤5例,坠落伤1例。受伤至入院时间在1~4 h。6例挫伤头皮均有不同程度泥土或碎屑等异物污染。     

对231例乙型肝炎伴肝功异常的学生的干预效果评价

目的研究心理干预对高校新生乙型肝炎病毒表面抗原携带者伴肝功能异常的作用,为他们的健康生活提供参考依据。方法将证实为乙型肝炎(下称乙肝)并伴肝功异常的高校新生随机分为两组,一组进行心理干预,对照组未进行心理干预。结果与对照组相比,进行心理干预的学生的肝功能和心理状况都有明显好转,差异有统计学意义(P<0.05)。结论对乙肝并伴有肝功异常的学生进行心理干预,可以明显地改善肝功能,提高在校的生活质量。     

珠海市部分体检人群血脂异常现状及护理干预进展

血脂异常是动脉粥样硬化性心脑血管疾病的首要危险因素,开展血脂检测,并积极采取降脂措施是心脑血管疾病初级预防的关键,通过健康促进等系统护理干预方案来预防和控制血脂异常,越来越受到人们的关注。本文对2008年1月至2010年12月本院体检的健康人群血脂进行检测分析及护理干预进行综述,现报道如下。     

新疆阿克苏地区重点人群碘营养和智力水平调查

Objective To investigate the iodine nutritional status of school children, lactating women and infants in iodine deficient areas of Baicheng and Wushi Counties in Xinjiang Autonomous Region. Methods According to the geographic location of east, south, west and north of county, 10 primary schools of 8 townships were selected. In each school, 10 children among each gender and age group from 8 to 10 years old were selected. A total of 300 school children were chosen. One hundred and four infants aged 0-2 years old and their mother were selected in 8 townships. Iodine content in edible salt at household level, the urinary iodine in school children and lactating women, total goiter rate(TGR) and the status of the intelligence quotient (1Q) of school children, the status of psychological development of infants were observed. Direct titration assay for testing the salt iodine, colorimetric ceric-arsenic assay and vitriolic ammonium assimilation were used for testing urinary iodine. The size of thyroid gland was measured by palpation. The Combined Raven Test for Chinese Rural was used to test the IQ. The psychological development of infants was tested by Danver Development Screening Test (DDST). Results The coverage rate of iodized salt at household level was 73.1% (123/182), however, the proportion of households using adequately iodized salt was 64.1% (118/182). The medium of urinary iodine in school children was 103.7 μ/L, with 47.8%(75/157) less than 100 μg/L and 28.0% (44/157) less than 50 μg/L; it was 123.0 μg/L in Baieheng County, with 44.4%(28/63) less than 100 μg/L and 33.3%(21/63) less than 50 μg/L; it was 100.3 μg/L in Wushi County, 50.0%(47/94) less than 100 μg/L and 24.5%(23/94) less than 50 μg/L. The medium of urinary iodine in locating women was 143.3 μg/L, it was 119.7 μg/L and 184.6 μg/L in Baicheng and Wushi Counties, respectively. The total rate of goiter in school children was 14.3%(43/300), it was 10.8%(13/120) and 16.6%(30/180) in Baicheng and Wushi Counties, respectively. The average IQ in school children was 80.6±11.6, it was 83.0±11.6 and 79.0±11.7 in Baicheng and Wushi Counties, respectively. The proportion of mental retardation in school children (IQ≤69) was 13.0% (39/300), it was 6.7% (8/120) and 17.2%(31/180) in Baicheng and Wushi Counties, respectively. In addition, the proportion of psychological development in infants being normal, suspicious and abnormal was 78.8%(82/104), 12.5% (13/104) and 8.7%(9/104), respectively. Conclusion This study confirms the fact that there is also existence of mental retardation in children and infants, caused by iodine deficiency.     

AFP、AFU和TSGF检测对原发性肝癌诊断的价值

甲胎蛋白(Alpha Fetoprotein,AFP)作为原发性肝细胞癌(primary hepatic cancer,PHC)主要的癌性标志物在临床应用已有30余年的历史,但我国肝癌患者有30～40%属AFP阴性.本研究旨在联合检测肝癌患者AFP、α-L-岩藻糖苷酶(AFU)和肿瘤肿瘤特异相关物质群(TSGF)的水平,比较三者诊断PHC阳性率和特异性的差异,及三者联合检测的诊断阳性率和特异性,进一步提高PHC的诊断水平.     

高通滤波器对多普勒血流信号的影响

在绝大多数连波多普勒血流测量系统中，通常采用一个高通滤波器来滤除由固定目标和血管壁产生的强反射信号。本文从理论上推导出引入高通滤波器会导致血流平均速率估值偏高。     

两种阻断方式对骶骨肿瘤切除术后伤口影响的比较

目的探讨选择性靶血管栓塞与球囊阻断2种方式对骶骨肿瘤术后患者伤口影响的比较。方法将104例骶骨肿瘤患者随机分为2组,术前行选择性靶血管栓塞(A组)53例,球囊导管腹主动脉阻断(B组)51例,其中20例患者术前已经行放、化疗。记录各组患者术中出血量、手术时间、术后引流量,观察2组患者术后伤口愈合情况。结果2组患者术中出血量、术后引流量相比差异无统计学意义(P0.05),2组患者手术时间、引流时间相比差异有统计学意义(P0.05)。A组皮肤红肿5例,愈合不佳8例,伤口窦道瘘口2例,皮肤坏死6例;皮肤大面积坏死2例;B组皮肤红肿1例,愈合不佳1例,伤口窦道瘘口1例;2组间比较,B组切口愈合不佳及皮肤坏死发生率高于A组,差异有统计学意义(P0.05)。随访2组患者术后0.5、1、2年肿瘤复发率差异无统计学意义(P0.05)。A组术后1例死亡、1例发生骶神经损伤,3例出现大小便功能障碍;B组术后无死亡,1例发生一过性神经症状,1例发生排尿功能障碍,术后1年左右恢复。结论球囊腹主动脉阻断可有效地控制骶骨肿瘤切除术中出血,与选择性靶血管栓塞相比对伤口影响更小。     

臀、股部吸脂术48例

肿胀吸脂术已逐渐成为美容门诊求美者最受欢迎的术式 ,而臀、股部吸脂又仅次于腹、腰部吸脂 ,位居第二。臀、股部吸脂有其特点 ,特别是与身材美化关系密切。国外已有关于股部吸脂和臀部吸脂的研究[1,2 ] ,而国内尚少见报道。自1999年 10月至 2 0 0 1年 3月 ,我们对 4 8例病人进行了臀、股部吸脂 ,效果满意 ,报道如下。一、资料与方法1.对象 :本组 4 8例 ,均为女性 ,年龄 19～ 4 5岁 ,均为臀部或股部臃肿 ,但脂肪沉着部位不同 ,其中髂嵴部臃肿 2 5例 ,大转子区臃肿 12例 ,臀部弥漫性脂肪堆积 3例 ,大腿弥漫性脂肪堆积 8例。2 .方法 :⑴术前测…     

进入体外受精-胚胎移植周期妇女应激情绪的研究

目的:探讨进入体外受精-胚胎移植(IVF-ET)周期妇女焦虑和抑郁情绪的变化特点。方法:征集46例将进行IVF-ET/胞浆内单精子注射(ICSI)的不孕症妇女,在进入治疗周期降调当日、取卵前1h、移植后12d等待人绒毛膜促性腺激素β亚单位(β-hCG)结果前这3个时间段的晨8:00～8:30间,在医院门诊采用状态焦虑和BECK抑郁量表进行心理测查并空腹采血。结果:①3个测试点妇女均有抑郁表现,但抑郁程度无差异。②状态焦虑均值在3个测试点有逐渐增高的趋势,测试点3较测试点1焦虑程度明显增高,有统计学差异(P<0.05)。③前3个测试点皮质醇激素水平显著高于正常参考值(P<0.01),测试点2和测试点3血浆皮质醇激素水平明显高于测试点1,并有统计学差异(P<0.05)。结论:进入IVF-EF周期妇女表现出较大的应激反应,并随治疗进展应激程度出现变化。应关注IVF妇女心理状况的变化。应以提高治疗的依从性和成功率。