Dermal penetration of creatine from a face-care formulation containing creatine, guarana and glycerol is linked to effective antiwrinkle and antisagging efficacy in male subjects

Background The dermal extracellular matrix provides stability and structure to the skin. With increasing age, however, its major component collagen is subject to degeneration, resulting in a gradual decline in skin elasticity and progression of wrinkle formation. Previous studies suggest that the reduction in cellular energy contributes to the diminished synthesis of cutaneous collagen during aging. Aims To investigate the potential of topically applied creatine to improve the clinical signs of skin aging by stimulating dermal collagen synthesis in vitro and in vivo. Patients/Methods Penetration experiments were performed with a pig skin ex vivo model. Effects of creatine on dermal collagen gene expression and procollagen synthesis were studied in vitro using cultured fibroblast‐populated collagen gels. In a single‐center, controlled study, 43 male Caucasians applied a face‐care formulation containing creatine, guarana extract, and glycerol to determine its influence on facial topometric features. Results Cultured human dermal fibroblasts supplemented with creatine displayed a stimulation of collagen synthesis relative to untreated control cells both on the gene expression and at the protein level. In skin penetration experiments, topically applied creatine rapidly reached the dermis. In addition, topical in vivo application of a creatine‐containing formulation for 6 weeks significantly reduced the sagging cheek intensity in the jowl area as compared to baseline. This result was confirmed by clinical live scoring, which also demonstrated a significant reduction in crow’s feet wrinkles and wrinkles under the eyes. Conclusions In summary, creatine represents a beneficial active ingredient for topical use in the prevention and treatment of human skin aging.     

露水草中的植物甾醇

 目的研究植物露水草中的化学成分。方法利用多种柱色谱进行分离提纯,通过理化常数和光谱数据确定化合物的结构。结果从中得到2个植物甾醇类化合物;分别为5α-胆甾-7-烯-3β,22ξ-二醇(Ⅰ),5α-胆甾-7-烯-22ξ-醇-3-棕榈酸酯(Ⅱ)。结论化合物Ⅰ为一新天然产物,Ⅱ为一新化合物。     

异位阑尾炎33例临床诊断分析

作者收集本院1994-01／2002-10期间异位阑尾炎33例，占本院阑尾炎收治总数3．37％，临床延误诊断分析如下。     

Evaluation of moxalactam with the BD phoenix system for detection of methicillin resistance in coagulase-negative staphylococci

The performance of moxalactam with the BD Phoenix system for the detection of methicillin resistance in coagulase-negative staphylococci was evaluated by use of a collection of 186 strains. Moxalactam was a better drug as an indicator of methicillin resistance for mecA-positive strains than oxacillin and cefoxitin were. For strains other than Staphylococcus saprophyticus, a moxalactam MIC >16 microg/ml was indicative of methicillin resistance.     

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??Abstract?? Objective To investigate the different clinical and immune features of variable phenotypes of severe combined immunodeficiency caused by RAG 1 mutations.Methods From 2012.9 to 2013.04?? three patients were included in the study??and records of clinical details were reviewed. Results The phenotypes of three patients were typical SCID for patient 1??Omenn syndrome for patient 2 and atypical SCID complicated with recurrent autoimmune hemolytic anemia for patients 3??respectively.RAG 1 mutations were compound heterozygous allele 1??1870 C??T/Arg624Cys??allele 2??2005 G??A/Glu669Lys??allele 1 was published mutation??allele 2 was de novel mutation?? for patient 1??compound heterozygous allele 1??994 C??T/Arg332X??allele 2??1439 G??A/Ser480Asn??both mutations were de novel mutations??for patient 2??homozygous 2095 C??T/R699W for patient 3 and was published mutation. First two patients died soon after discharge.Patient 3 was treated for recurrent autoimmune hemolytic anemia in our ward.Conclusion RAG1 mutations can lead to variable SCID phenotypes.Patients with typical SCID and Omenn syndrome were with poor prognosis??which need transplantation treatment.     

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??Objective??To investigate the correlation between obesity and periodontitis in middle aged and old population. Methods??The study sample included 381 middle-aged and elderly people from the Physical Examination Center of the First Affiliated Hospital of Xinjiang Medical University??questionnaire survey??general physical examination and oral cavity examination were conducted in them??record the calculus index??CI????bleeding on probing??BOP????periodontal pocket probing depth??PD????clinical attachment loss??CAL????body mass index??BMI??and level of four items of blood lipid tests. Results??The triglyeride level was significantly higher in the obese group??P??0.05????and the high-density lipoprotein cholesterol level was significantly lower in the obese group??P??0.05????as compared with normal group. Analysis results showed that BMI was a risk factor for periodontitis??OR??1??P??0.05????but there was no obvious correlation between them??P??0.05??. Calculus index??bleeding on probing and clinical attachment loss were positively correlated with BMI in middle-aged??P??0.05??. There was no correlation between BMI and periodontal pocket probing depth or attachment loss??P??0.05??.  Conclusion??Obesity is a risk factor for periodontitis in middle-aged and elderly people??but the correlation between them is still uncertain.     

Susceptibility genes and modifiers for cardiac arrhythmias

The last decade has seen a dramatic increase in the understanding of the molecular basis of arrhythmias. Much of this new information has been driven by genetic studies that focused on rare, monogenic arrhythmia syndromes that were accompanied or followed by cellular electrophysiological or biochemical studies. The marked clinical heterogeneity known from these familial arrhythmia syndromes has led to the development of a multifactorial ("multi-hit") concept of arrhythmogenesis in which causal gene mutations have a major effect on disease expression that is further modified by other factors such as age, gender, sympathetic tone, and environmental triggers. Systematic genetic studies have unraveled an unexpected DNA sequence variance in these arrhythmia genes that has ethnic-specific patterns. Whether this genetic variance may contribute as a second genetic modifier for arrhythmia development is under current investigation. The aim of this article is to review common genetic variation in ion channel genes and to compare these recent findings.     

氢氟酸酸蚀对两种树脂-陶瓷复合材料黏结强度影响研究

目的    研究氢氟酸酸蚀对两种树脂-陶瓷复合材料黏结强度的影响。方法    选择Hyramic和Vita Enamic两种树脂-陶瓷复合材料,每种材料再按照表面处理方式分为未处理组（Hyramic-NT组和Vita Enamic-NT组）以及氢氟酸酸蚀组（Hyramic-HF组和Vita Enamic-HF组）。测量表面处理后的复合材料表面粗糙度,扫描电镜观察表面形貌。使用Multilink N树脂水门汀将组内的复合材料两两黏结,制作微拉伸试件,37℃去离子水中水浴24 h后进行微拉伸强度测试。将粗糙度及微拉伸黏结强度结果采用SPSS 22.0软件进行两因素析因设计资料的方差分析。结果　不同的表面处理方式对微拉伸黏结强度和粗糙度有影响（均P < 0.05）,材料种类对微拉伸黏结强度和粗糙度无明显影响（均P > 0.05)。Hyramic-HF组微拉伸黏结强度高于Hyramic-NT组,但差异无统计学意义（P > 0.05）。Vita Enamic-HF组微拉伸黏结强度明显高于Vita Enamic-NT组,差异有统计学意义（P < 0.05）。粗糙度及扫描电镜结果显示,氢氟酸处理后复合材料的表面形貌有明显改变。结论    氢氟酸酸蚀可提高树脂-陶瓷复合材料与树脂水门汀之间的黏结强度,Vita Enamic比Hyramic更适合氢氟酸酸蚀处理。     

老年科住院患者抗抑郁药物应用77例分析

目的 探讨老年人应用抗抑郁药的安全性,为老年人合理使用抗抑郁药提供参考。方法 回顾性分析2012年1月至2013年12月北京协和医院院老年示范病房77例65岁以上患者的抗抑郁药物的使用情况、起始剂量及不良反应。结果 经老年医学跨学科团队会诊,77例均服用新型抗抑郁药,其中舍曲林使用率最高,对低体重指数患者更多选择米氮平治疗,伴有慢性疼痛的患者中50%使用5-羟色胺和(或)去甲肾上腺素再摄取抑制剂。70例住院后开始抗抑郁药治疗,72.8%的患者初始给药量从小剂量开始,不良反应发生率为8.6%(6/70)。结论 应结合老年患者特点,以及抗抑郁药的特点、不良反应和药物之间的相互作用来选择适当的抗抑郁药物。