全文获取类型
收费全文 | 692篇 |
免费 | 67篇 |
国内免费 | 35篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 2篇 |
妇产科学 | 2篇 |
基础医学 | 77篇 |
口腔科学 | 4篇 |
临床医学 | 47篇 |
内科学 | 81篇 |
皮肤病学 | 2篇 |
神经病学 | 18篇 |
特种医学 | 13篇 |
外科学 | 90篇 |
综合类 | 174篇 |
预防医学 | 50篇 |
眼科学 | 16篇 |
药学 | 112篇 |
2篇 | |
中国医学 | 57篇 |
肿瘤学 | 46篇 |
出版年
2024年 | 9篇 |
2023年 | 17篇 |
2022年 | 36篇 |
2021年 | 40篇 |
2020年 | 22篇 |
2019年 | 24篇 |
2018年 | 25篇 |
2017年 | 20篇 |
2016年 | 18篇 |
2015年 | 33篇 |
2014年 | 32篇 |
2013年 | 31篇 |
2012年 | 36篇 |
2011年 | 28篇 |
2010年 | 30篇 |
2009年 | 24篇 |
2008年 | 21篇 |
2007年 | 39篇 |
2006年 | 32篇 |
2005年 | 36篇 |
2004年 | 23篇 |
2003年 | 25篇 |
2002年 | 25篇 |
2001年 | 13篇 |
2000年 | 12篇 |
1999年 | 19篇 |
1998年 | 8篇 |
1997年 | 14篇 |
1996年 | 16篇 |
1995年 | 13篇 |
1994年 | 11篇 |
1993年 | 17篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 9篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 3篇 |
排序方式: 共有794条查询结果,搜索用时 31 毫秒
51.
Sulfite (bisulfite/sulfite) can affect voltage-gated sodium (Na+) channels (VGSC) in a concentration-dependent manner in isolated rat ventricular myocytes. In this study, the effect of epigallocatechin-3-gallate
(EGCG) on VGSC in isolated ventricular myocytes was studied. Ventricular myocytes were exposed to 10 μM bisulfite/sulfite
for 10 min, and EGCG was then administered in different concentrations (10, 30, 50 μg ml−1). Decreased activity of superoxide dismutase, catalase (CAT) and glutathione peroxidase (GPx) was observed after bisulfite/sulfite
exposure, with significant increase in Na+ currents (I
Na) and alterations in half-activation voltage and half-inactivation voltage. Intracellular reactive oxygen species (ROS) such
as hydrogen peroxide (H2O2), hydroxyl (OH·), and superoxide anion (O2·−) were increased. After EGCG treatment, activity of the aforementioned enzymes increased while the ROS level decreased. The
effects progressed with increasing amounts of EGCG, up to a level similar to blank control at the dose of 50 μg ml−1 EGCG, EGCG also reduced the I
Na and reversed the alterations in half-activation voltage and half-inactivation voltage. In conclusion, EGCG could protect
Na+ channels in rat ventricular myocytes against the oxidative damage induced by sulfite as a scavenger of the ROS. 相似文献
52.
徐州地区1572例健康人群骨密度和骨质疏松症调查 总被引:2,自引:0,他引:2
目的通过测量徐州地区健康人群骨密度(BMD),分析骨密度的变化规律及骨质疏松症的发病率,提高对骨质疏松症诊断的可靠性,为骨质疏松症的防治提供参考依据。方法采用GE-LUNAR公司生产的双能X线骨密度仪对徐州地区2005~2008年1572例健康人进行BMD测定,分别做股骨上端及第2~4腰椎椎体测量,并按5岁为一个年龄组分组。以峰值BMD减低2.5SD为诊断骨质疏松症标准,按性别、年龄分组进行统计学分析。结果徐州地区女性BMD峰值则在30~35岁,男性在35岁左右,女性55岁后骨量下降较男性显著,骨质疏松发病率女性高于男性。结论对于55岁女性和65岁男性,之前应加强普及骨质疏松预防知识,之后同时采取相应干预措施,以减少骨质疏松症的发生,为徐州地区骨质疏松症的诊治提供参考依据。 相似文献
53.
目的 探讨脂多糖(LPS)对RAW264.7细胞白细胞介素10(interleukin-10,IL- 10)分泌及mRNA表达的影响.方法 采用体外培养巨噬细胞株RAW264.7细胞,实验分为正常对照组、LPS组.采用ELISA法、逆转录PCR(RT - PCR)技术检测IL- 10分泌及mRNA表达的变化.结果 LPS刺激RAW264.7细胞后IL- 10的分泌及mRNA表达较对照组增加(P<0.05),并具有时间依赖性.结论 LPS可刺激RAW264.7细胞IL- 10分泌及mRNA表达的增加. 相似文献
54.
目的探讨纤溶酶原激活物抑制物-1(PAI-1)基因4G/5G多态性与冠状动脉疾病的关系。方法采用病例对照研究的方法。病例组146例,72例行冠状动脉造影证实患有CAD;正常对照组113例,选自人群健康体检者,所有入选病例均排除CAD史,脑卒中短暂性脑缺血发作病史及相似病史,排除周围血管疾病史。均用PCR-RFLP的方法判断各组基因型,最后进行统计学分析。结果与5G/5G相比,4G/5G,4G/4G的OR呈增加趋势,提示4G等位基因与冠心病的危险性相关。具有4G/4G基因型比具有5G/5G基因型的人患冠心病的危险性增加2.06倍(95%CI:1.03~4.14)。结论 PAI-1基因4G/4G基因型与中国汉族人群冠心病发生相关。 相似文献
55.
56.
Guo Y Jankovic J Song Z Yang H Zheng W Le W Tang X Deng X Yang Y Deng S Luo Z Deng H 《Neuroscience letters》2011,487(2):174-176
Essential tremor (ET) has been hypothesized to be a risk factor for the development of Parkinson disease (PD). Recently, rs9652490 variant in the leucine-rich repeat and Ig domain containing 1 gene (LINGO1) was found to be associated with ET susceptibility. To evaluate whether the same variant is associated also with PD susceptibility, we investigated the association between the LINGO1 rs9652490 variant and PD phenotype in Caucasian and Chinese PD subjects. We found no significant differences in genotypic and allele distribution between patients and control subjects (χ(2)=1.931, p=0.381 for genotypic distribution; χ(2)=0.001, p=0.973 for allele distribution), suggesting this variant is not associated with PD. 相似文献
57.
Immune thrombocytopenic purpura (ITP) is an autoimmune disorder, and high-dose dexamethasome (HD-DXM) has been used as a first-line therapy for patients with ITP. However, little is known about the role of dendritic cells (DCs) and CD4(+)Foxp3(+) regulatory T (Treg) cells in the pathogenesis of chronic ITP and the effects of HD-DXM on DCs and Treg cells. In this study, we investigated the amounts of circulating myeloid DCs (mDCs), plasmacytoid DCs (pDCs), and CD4(+)Foxp3(+) Treg cells in 26 untreated adult patients with chronic ITP. All patients had thrombocytopenia (platelet count <50 x 10(9)/L) for more than 6 months. We also observed short-time changes of DCs and Treg cells after treatment with HD-DXM in these patients. Both mDCs and pDCs numbers in patients were comparable with that of healthy controls. In contrast, the percentage of Treg cells was significantly reduced in patients when compared with healthy controls (P < 0.0001). After 4-days treatment with HD-DXM, Treg cells and mDCs were increased (P < 0.0001 and P < 0.05), while pDCs decreased (P < 0.0001), and CD11c expression level in mDCs was downregulated (P < 0.0001). These results suggest that Treg cells are deficient in ITP and the immunosuppressive therapy of glucocorticoids could cause the short-time changes of these cells. 相似文献
58.
Maritza Zavaleta-Pastor Christian Sohlenkamp Jun-Lian Gao Ziqiang Guan Rahat Zaheer Turlough M. Finan Christian R. H. Raetz Isabel M. López-Lara Otto Geiger 《Proceedings of the National Academy of Sciences of the United States of America》2010,107(1):302-307
Rhizobia are Gram-negative soil bacteria able to establish nitrogen-fixing root nodules with their respective legume host plants. Besides phosphatidylglycerol, cardiolipin, and phosphatidylethanolamine, rhizobial membranes contain phosphatidylcholine (PC) as a major membrane lipid. Under phosphate-limiting conditions of growth, some bacteria replace their membrane phospholipids with lipids lacking phosphorus. In Sinorhizobium meliloti, these phosphorus-free lipids are sulfoquinovosyl diacylglycerol, ornithine-containing lipid, and diacylglyceryl trimethylhomoserine (DGTS). Pulse–chase experiments suggest that the zwitterionic phospholipids phosphatidylethanolamine and PC act as biosynthetic precursors of DGTS under phosphorus-limiting conditions. A S. meliloti mutant, deficient in the predicted phosphatase was unable to degrade PC or to form DGTS in a similar way as the wild type. Cell-free extracts of Escherichia coli, in which SMc00171 had been expressed, convert PC to phosphocholine and diacylglycerol, showing that SMc00171 functions as a phospholipase C. Diacylglycerol , in turn, is the lipid anchor from which biosynthesis is initiated during the formation of the phosphorus-free membrane lipid DGTS. Inorganic phosphate can be liberated from phosphocholine. These data suggest that, in S. meliloti under phosphate-limiting conditions, membrane phospholipids provide a pool for metabolizable inorganic phosphate, which can be used for the synthesis of other essential phosphorus-containing biomolecules. This is an example of an intracellular phospholipase C in a bacterial system; however, the ability to degrade endogenous preexisting membrane phospholipids as a source of phosphorus may be a general property of Gram-negative soil bacteria. SMc00171相似文献
59.
目的探讨非同步螺旋CT辅助定位盲穿建立皮肤肾脏通路,行微创经皮肾穿刺输尿管镜取石术(MPCNL)治疗嵌顿性输尿管上段结石的可行性、安全性和临床疗效。方法对102例单侧嵌顿性输尿管上段结石患者,采用非同步螺旋CT辅助定位盲穿MPCNL治疗。结果102例均Ⅰ期成功穿刺建立皮肤肾脏通路,Ⅰ期成功碎石,结石清除率为100%,手术时间平均55min,术中估计出血量平均30mL。无气胸、大出血、输尿管穿孔、腹腔脏器损伤等严重并发症发生。结论非同步螺旋CT辅助定位盲穿MPCNL治疗嵌顿性输尿管上段结石方便可行、安全高效。 相似文献
60.