LINGO1 rs9652490 variant in Parkinson disease patients

Essential tremor (ET) has been hypothesized to be a risk factor for the development of Parkinson disease (PD). Recently, rs9652490 variant in the leucine-rich repeat and Ig domain containing 1 gene (LINGO1) was found to be associated with ET susceptibility. To evaluate whether the same variant is associated also with PD susceptibility, we investigated the association between the LINGO1 rs9652490 variant and PD phenotype in Caucasian and Chinese PD subjects. We found no significant differences in genotypic and allele distribution between patients and control subjects (χ(2)=1.931, p=0.381 for genotypic distribution; χ(2)=0.001, p=0.973 for allele distribution), suggesting this variant is not associated with PD.     

腹腔镜右半结肠癌根治术中使用血管结扎束34例病例报告

目的探讨腹腔镜右半结肠癌根治术中使用血管结扎束(Liga Sure)进行手术的效果及短期安全性评价。方法回顾性分析2013年11月至2017年2月使用血管结扎束进行的34例腹腔镜右半结肠癌根治术患者的临床资料。收集患者术中、术后资料,分析患者使用血管结扎束的效果及安全性。结果纳入的34例患者均顺利完成手术,手术时间(174.0±28.3)min,出血量(34.0±33.5)ml,无术中并发症,无损伤肠系膜上血管主干病例发生;2例患者发生乳糜漏,2例患者出现系膜破损,术后平均住院时间(6.0±4.6)d。平均获检淋巴结数量为24枚,阳性约1.2枚,共有15例患者伴有淋巴结转移。结论通过上述结果,使用Liga Sure进行的病例中无严重并发症发生,出血少,手术时间短,Liga Sure可用于腹腔镜右半结肠癌根治术的游离,短期效果及安全性均较好。     

神经内镜治疗自发性脑室出血的疗效分析

目的探讨神经内镜对自发性脑室出血手术治疗的疗效分析。方法收集2009年6月至2013年12月我院经CTA筛查的119例自发性脑室出血患者,分为2组:神经内镜手术组(NEG)48例、脑室外引流组(EVDG)71例,手术治疗均在48小时内进行;比较二组不同术式术后6小时颅脑cT,血肿清除率、术后并发症及术后6个月对患者进行ADIL预后分级等治疗效果评估。结果①血肿清除率:NEG明显优于EVDG(p0.01)。②术后片发症:发生率分别为1 2.5%、40.6%。NEG明显低于EVDG(p=0.048)。③术后6月ADI分级:NEG和EVDG术后6个月ADL预后评分恢复良好(ADL.Ⅰ~Ⅲ级)比例分别为:79.17%(38/48)、46.48%(33/71)。NEG术后6月ADL评分明显优于EVDG(p=0.001)。结论神经内镜组手术血肿清除率高、并发症少、预后好,优于脑室外引流手术组。     

Investigation of urinary components in rat model of ketamine-induced bladder fibrosis based on metabolomics

BackgroundKetamine abuse has been linked to the system''s damage, presenting with lower urinary tract symptoms (LUTS). While the pathogenesis of ketamine-induced urinary damage is not fully understood, fibrosis is believed to be a potential mechanism. A metabolomic investigation of the urinary metabolites in ketamine abuse was conducted to gain insights into its pathogenesis.MethodsA rat model of ketamine induced bladder fibrosis was established through tail vein injection of ketamine hydrochloride and control group was established through tail vein injection of the equivalent normal saline. Hematoxylin and eosin (H&E) staining and Masson trichrome staining were performed to evaluated bladder pathology. Urinary components were detected based on a metabolomic approach using ultra-high performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOFMS platform). Orthogonal projections analyzed the data to latent structures discriminant analysis (OPLS-DA) and bioinformatics analysis.ResultsThe rat model of ketamine induced bladder fibrosis was confirmed through H&E and Masson trichrome staining. There were marked differences in the urinary metabolites between the experimental group and the control group. Compared to the control group, 16 kinds of differential metabolites were up-regulated and 102 differential metabolites were down-regulated in the urine samples of the ketamine group. Bioinformatics analysis revealed the related metabolic pathways.ConclusionsUsing a ketamine-induced bladder fibrosis rat model, this study identified the differential urinary metabolites expressed following ketamine treatment. These results provide vital clues for exploring the pathogenesis of ketamine-induced LUTS and may further contribute to the disease''s diagnosis and treatment.     

贲门癌切除术后残胃排空功能变化

目的 观察贲门癌术后残胃排空功能变化,探讨改善病人术后生活质量的方法。方法 用豆奶粉４０ｇ,水２００ｍＬ,加入１８．５ＭＢｑ＾９９Ｔｃ＾ｍ－ＤＴＰＡ,制成试验餐,于术后１８￣２０ｄ例行贲门癌近端胃次全切除患者,和５例健康人（Ｎ组）进行核素胃排空检查。     

氢溴酸山莨菪碱肌内注射联合推拿治疗发作期梅尼埃病 79例临床观察

目的探讨氢溴酸山莨菪碱注射液联合推拿治疗发作期梅尼埃病的有效性和安全性。方法将 2017年 8月至 2019年 5月武警四川省总队医院发作期梅尼埃病病人 158例按随机数字表法分为试验组和对照组,每组 79例。对照组给予氢溴酸山莨菪碱注射液,肌内注射,首日剂量 10毫克 /次, 1次/天,次日开始 20毫克 /次, 1次/天, 7d为 1个疗程,连续使用 2个疗程。试验组在对照组基础上,加用推拿。比较两组病人经治疗两周后的总有效率及头晕残障问卷(DHI)、耳鸣致残量表(THI)、耳闷视觉模拟评分(VAS)评分,以及不良反应。结果经治疗 2周后,试验组总有效率高于对照组,差异有统计学意义(93.67%比 82.28%,χ2＝6.939,P＝0.031);试验组的 DHI、THI、VAS评分较对照组更低［DHI:(35.39±4.37)分比(38.25±8.58)分, t＝2.640, P＝0.009;THI:(37.54±7.37)分比(41.37±8.94)分, t＝2.938,P＝0.004;VAS:(5.46±1.89)分比(6.39±2.11)分, t＝2.918,P＝0.004］。在不良反应方面,试验组出现 5例潮红、口干,对照组出现 6例潮红、口干,均未经干预, 2～4h后自行消失,余未见不良反应,两组不良反应发生率差异无统计学意义(χ2＝0.098,P＝0.755)。结论推拿联合氢溴酸山莨菪碱注射液治疗发作期梅尼埃病较单用氢溴酸山莨菪碱注射液更为有效,安全性佳,无严重不良反应发生,可考虑作为临床治疗梅尼埃病的一种治疗方案。     

肝阳上亢证甲皱微循环观察与分析

以我所统一标准,确诊为“肝阳上亢证”者,进行微循环观察,肝阳上亢证者90例;阴虚阳上亢证者88例,健康对照组48例。观察发现肝阳上亢组与健康对照组及阴虚阳亢组与健康对照组均有显著性差异。通过卡方证明本检测数据可靠,微循环观测可作为“肝阳上亢证”的客观检测指标之一。     

青少年特发性脊柱侧凸柔韧性影响因素分析及仰卧侧屈角度的预测

目的分析青少年特发性脊柱侧凸柔韧性影响因素,探讨预测指标,初步建立仰卧侧屈位Cobb角角度的预测模型。方法通过对青少年特发性脊柱侧凸150例患者（包括胸椎侧凸和胸腰段/腰椎侧凸共216个）的站立位脊柱全长正位X线片和仰卧侧屈位X线片资料进行回顾性研究,用仰卧侧屈位X线片矫正率作为柔韧性指标,与站立位冠状面Cobb角、年龄、性别、Risser征、是否主侧凸和侧凸位置6个指标进行Pearson或Spearman相关分析及多元线性回归,筛选相关因素;采用同样方法 ,分析6个指标与仰卧侧屈Cobb角的关系。结果站立位冠状面Cobb角（P〈0.01）和侧凸位置（P〈0.01）与柔韧度存在明显线性回归关系。站立位冠状面Cobb角（P〈0.01）、侧凸位置（P〈0.01）和是否主侧凸（P〈0.01）与仰卧侧屈Cobb角存在明显的线性回归关系。对青少年特发性脊柱侧凸,站立位冠状面Cobb角每增加10°,柔韧度约减少8%,胸椎侧凸柔韧性平均比胸腰段/腰椎侧凸低10%。站立位冠状面Cobb角〉45°的胸椎次侧凸和〉50°的胸腰段/腰椎次侧凸,成为结构性侧凸几率较大。结论站立位冠状面Cobb角和侧凸位置是显著影响青少年特发性脊柱侧凸柔韧度的因素,实验探讨了1种预测仰卧侧屈Cobb角角度的简单方法 ,解决了临床实际问题。     

慢性肾功能衰竭正虚诸证与实验室指标的关系研究

慢性肾功能衰竭（CRF,简称慢性肾衰）是各种原发或继发肾脏病晚期的一种共同归宿,是一组进行性肾单位毁损,以肾脏的排泄功能,内环境稳定功能和内分泌功能紊乱为特征的临床综合征。在古医籍中未见专门的论述,尚难找到完全对应的病名,从其临床表现特征及发生发展过程来看,类似于中医学中的关格、肾风、癃闭、溺毒等病,国家标准《中医临床诊疗术语》现正式命名为“肾衰病”。近些年来,随着腹膜透析、血液透析及肾移植的推广及技术完善,CRF患者的生存质量明显改善,但昂贵的医疗费用及紧缺的肾源给国民经济及患者家庭带来沉重的负担。