3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用研究

目的探讨3D打印胶原/羟基磷灰石支架对骨髓间充质干细胞成骨分化的作用。 方法分离SPF级雄性SD大鼠骨髓间充质干细胞,实验分为：对照组、浸提组、诱导组及浸提诱导组,MTT法检测对照组,浸提组的细胞增殖情况,对比分析各组细胞的碱性磷酸酶（ALP）活性,对各组细胞诱导培养17天后进行茜素红染色,观察钙结节染色情况。 结果MTT结果显示,浸提组与对照组在不同时间点的OD值比较,差异无统计学意义（P>0.05）,ALP活性结果显示,不同时间点各组与对照组相比,差异均有统计学意义（P<0.05）;不同时间点浸提诱导组与浸提组相比,差异均有统计学意义（P<0.05）;诱导组在48 h及72 h与浸提组相比,差异均有统计学意义（P<0.05）。茜素红染色结果显示,对照组细胞无钙结节点,浸提组及诱导组镜下肉眼可明显观察到红色区域染色,镜下观察可见钙结节点,浸提诱导组所产生的钙结节点的数量、大小以及染色的颜色深度均明显优于其他各组。 结论3D打印胶原/羟基磷灰石支架具有生物相容性好,可促进BMSCs向成骨分化,对细胞毒性低等特点,适宜用作骨缺损的治疗。     

中国人群维生素D受体rs7975232和rs1544410位点基因多态性与骨质疏松症相关性的meta分析

目的本研究采用meta分析的方法,探讨了中国人维生素D受体基因多态性与骨质疏松的关系。 方法检索CNKI、万方、维普、Pubmed、Web of Science、Embase数据库,检索时间从建库至2020年3月,从数据库中检索有关维生素D受体（rs7975232和rs1544410）基因多态性和骨质疏松症之间相关性的文章。采用NOS文献质量评价标准对符合纳入排除标准的文献进行质量评价。采用OR值及其95%可信区间（95% CI）评估关联强度。根据是否存在异质性,采用固定效应模型或随机效应模型合并效应量。采用单因素敏感性分析法进行敏感性分析。 结果本研究最终共纳入20项符合条件的研究,包括4 468例病例和4 988名对照。在等位基因模型中,rs7975232和rs1544410的合并OR估计值分别为1.09（95% CI：0.84,1.39,P=0.524）和0.9（95% CI：0.57,1.43,P=0.659）,尚未发现两个位点与骨质疏松症的关联。在显性模型、隐性模型和共显性模型中,结果均未显示二者的相关性。 结论在中国人群中,维生素D受体（rs7975232和rs1544410）基因多态性与骨质疏松风险之间无关联。     

Bioinformatic gene analysis for potential biomarkers and therapeutic targets of diabetic nephropathy associated renal cell carcinoma

BackgroundNumerous epidemiological studies have confirmed that diabetes can promote the development of malignant tumors. However, the relationship between renal cell carcinoma (RCC) and diabetic nephropathy (DN) is still controversial. This study aimed to investigate the genes that are co-expressed in DN and RCC in order to gain a better understanding of the relationship between these diseases, and to identify potential biomarkers and targets for the treatment of DN-related RCC.MethodsWe evaluated the differentially expressed genes (DEGs) that are co-expressed in DN and RCC using a wide range of target prediction and analysis methods. Twenty-four genes were identified by intersecting the differential genes of 3 DN datasets and 2 RCC datasets. We predicted the micro-ribonucleic acids (miRNAs) of these genes that may be controlled using the miRNA Data Integration Portal (mirDIP) database, and rated them according to each data forecast based on the Comparative Toxicogenomics Database (CTD) and the StarBase database.ResultsFour genes were associated with DN and RCC patients: the predicted miRNAs hsa-miR-200b-3p and hsa-miR-429 of fibronectin 1 (FN1); the predicted miRNA hsa-miR-29c-3p of collagen type 1 alpha 2 (COL1A2); the predicted miRNA hsa-miR-29c-3p of collagen type 3 alpha 1 (COL3A1); and the predicted miRNA hsa-miR-29a-3p and hsa-miR-200c-3p of glucose-6-phosphatase catalytic subunit (G6PC). These genes may serve as potential biomarkers or specific targets in the treatment of DN-related RCC.ConclusionsA significant correlation was identified between DN and RCC. The FN1, COL1A2, COL3A1, and G6PC genes could be novel biomarkers of DN-related RCC.     

The effect of intradialytic combined exercise on hemodialysis efficiency in end-stage renal disease patients: a randomized-controlled trial

Purpose

Insufficient dialysis is a difficult problem for patients undergoing hemodialysis, and causes cardiovascular complications and increases mortality. Increasing aerobic exercise and resistance exercise have been shown to be beneficial to physical fitness of patients undergoing hemodialysis, but a few studies have focused on combined exercise (combination of aerobic and resistance exercise training) and the interaction effect of combined exercise and intervention duration on hemodialysis efficiency. This study aimed to investigate the effects of 24-week combined exercise on hemodialysis efficiency, blood pressure, exercise capacity, and quality of life in patients undergoing hemodialysis.

Methods

In total, 47 eligible subjects were randomly allocated to exercise group and control group. The intervention group performed a 24-week, three times weekly, and moderate-intensity intradialytic combined exercise. Patients in the control group received usual care. The primary outcome was hemodialysis efficiency, which recorded every 4 weeks. Secondary outcomes included blood pressure, exercise capacity, and quality of life, measured at baseline and after 24 weeks of intervention.

Results

In intervention group, sp Kt/V significantly improved by 13.2%, and systolic blood pressure and diastolic blood pressure significantly decreased by 8.5 mmHg and 6.5 mmHg, respectively. The 6-min walking distance increased significantly by 43 m (9.8%), but there was no significant change in quality of life.

Conclusion

Combined exercise and intervention duration had an interaction effect on hemodialysis efficiency. Combined exercise improved blood pressure and physical fitness for patients undergoing hemodialysis, but did not affect quality of life. The extensive benefits of combined exercise provide evidence for the exercise development for patients undergoing hemodialysis.

     

Studies on the inhibitory models of pyrazoline derivatives as EGFR kinase inhibitors by 3D-QSAR and molecular docking

The development of EGFR kinase inhibitors attracts much attention of research for treating of cancer in recent years. In this work, based on a dataset composed of 46 EGFR kinase inhibitors, the combination of three-dimensional quantitative structure–activity relationship (3D-QSAR), molecular docking was applied to reveal structural characteristics impacting the inhibitory activity of EGFR, and to provide a better understanding of the binding modes between inhibitors and EGFR kinase. 3D-QSAR models of pyrazoline derivatives were established to reveal how steric, electrostatic, hydrophobic, and H-bond acceptor interactions contribute to inhibitors’ bioactivities, which were unanimous in the docking results. Furthermore, based on the most active compound, several new molecules with high inhibitory activity were obtained.     

全胃多发类癌一例

1 临床资料 患者女,46岁。因进食后腹胀小适1年余,期间无腹痛、恶心、呕吐、反酸、暖气、呕血、黑便等症状,于当地乡镇卫生院就诊,行上消化道钡餐造影检查,诊断为慢性萎缩性胃炎,给予促胃肠动力、抑酸、保护胃黏膜等保守治疗后症状缓解。此后反复发作腹胀不适,为求进一步诊断与治疗患者于2012年8月28日来我院普通外科就诊。患者自述慢性萎缩性胃炎病史10余年、体格检查：胸部和腹部均无阳性体征。     

A comparison of biomechanical changes on femoral head following rotational acetabular osteotomy and eccentric rotational acetabular osteotomy in normal cadaveric hip

Purpose

Both rotational acetabular osteotomy (RAO) and eccentric rotational acetabular osteotomy (ERAO) are effective procedures for young patients with developmental dysplasia of the hip. However, no comparative study of biomechanical changes has been reported following these two procedures. We therefore explored the stress changes on femoral head after RAO and ERAO under different load conditions.

Materials and methods

Twelve female cadaveric hips without deformity were divided into RAO group and ERAO group. Stress value on femoral head was measured preoperatively and postoperatively after the vertical force was loaded on the cadaveric spine from 0 to 500 N. Stress change value was then calculated base on the measurements.

Results

In the RAO group, preoperative stress increased when loading on spine became larger, but postoperative stress changed its increasing trend into decreasing when the load was greater than 200 N (turning point). Same phenomenon was found in the ERAO group (turning point was 300 N). However, the difference between preoperative and postoperative stress was not statistically significant in both RAO and ERAO groups. Stress change value from each procedure showed similar trends. With the load growth, stress change increased firstly and then decreased, but the difference between RAO and ERAO was not statistically significant.

Conclusions

Both RAO and ERAO could correct the abnormal biomechanical effect of dysplastic hip; moreover, they may have similar biomechanical effects on femoral head, obtaining the same clinical outcomes. Non-biomechanical factors (surgical trauma, technical complexity, etc.) also play important roles in procedure selection.     

Association of nitric oxide synthase 3 (NOS3) 894 G>T polymorphism with prognostic outcomes of anthracycline in Chinese patients with acute myeloid leukaemia

The aim of the present study was to investigate the influence of the nitric oxide synthase 3 (NOS3) 894 G>T polymorphism on prognostic outcomes of anthracycline in Chinese patients with de novo intermediate‐risk acute myeloid leukaemia (AML) and to examine the gene expression level in relation to genetic variation. In all, 225 Chinese patients with intermediate‐risk AML (at the complete remission stage) treated with anthracycline were enrolled in the study. The 894 G>T polymorphism of the NOS3 gene was analysed by allele‐specific matrix‐assisted laser desorption ionization time‐of‐flight. Expression of NOS3 mRNA was tested in 72 patients of known genotype for NOS3 894 G>T. The clinical characteristics of these patients were obtained from medical records. Survival analysis showed that patients with AML (GG genotype) had a longer overall survival (OS; P = 0.006). After adjusting for age, gender, leucocyte count, haemoglobin level, platelet level, French, American and Britain (FAB) classification, lactate dehydrogenase levels, Eastern Cooperative Oncology Group Performance Status, nucleophosmin gene and fms‐related tyrosine kinase 3 gene, multivariate survival analysis showed that the NOS3 894 G>T polymorphism appeared to be a predicting factor for OS (P = 0.014; hazard ratio = 1.856). However, no significant associations between the NOS3 894 G>T polymorphism and relapse‐free survival and relapse in patients with AML were observed. Gene expression levels were significantly higher in patients with the GG genotype than in patients with the GT and TT genotypes (P = 0.033). The findings suggest that the NOS3 894 G>T variant may be a biomarker for the prediction of OS in Chinese patients with AML.     

眼黏膜相关淋巴瘤并骨髓侵犯一例伴文献分析

目的观察眼附属器黏膜相关淋巴瘤的临床特点。方法报告1例双眼同时起病的眼附属器黏膜相关样淋巴组织（MALT）结外边缘区B细胞淋巴瘤（OAML）并骨髓侵犯一例,结合文献对该类型淋巴瘤病因、临床表现、免疫组化、分子生物学改变、治疗进行探讨。结果OAML大部分为局限性病变,单侧多见,晚期播散型少见。结论OAML为惰性过程,相关至死率低,对于IE可给予放疗,加用免疫化学治疗可降低复发,对于IVE期患者需全身化疗,总体预后良好。