复方中药对肝纤维化治疗前后的血清学评价

目的通过对肝纤维化复方中药治疗的前后对比,结合肝病理组织学改变,进一步了解血清纤维化指标的诊断价值。方法对临床确诊为慢性乙肝及早期肝硬化的３４例患者,服用复方中药制剂半年,治疗前后各肝穿一次及采血４次,血清检测透明质酸（ＨＡ）,Ⅲ型前胶原（ＰＣⅢ）Ⅳ型胶原（ＣＩＶ）及层粘素（ＬＮ）。ＨＡ、ＰＣⅢ及ＬＮ用放免法,ＣＩＶ用酶免法。结果治疗前后对比,血清纤维化指标中,ＨＡ、ＰＣⅢ及ＩＮ变化非常显著（Ｐ＜０００１）;ＣＩＶ变化不明显（Ｐ＞００５）;血清纤维化四项指标与病理炎症及纤维化程度关系密切。与炎症计分的相关系数为：ＰＣⅢ：（０５２８５）、ＨＡ（０３８７７）、ＣＩＶ（０３４６４）和ＬＮ（０３３４１）;与纤维化计分的相关系数为：ＨＡ（０６２０３）、ＬＮ（０４２８９）、ＰＣⅢ（０４０９８）和ＣＩＶ（０３９７７）。结论血清纤维化指标为较好反映肝纤维化的指标,并随治疗有效而下降,其中ＨＡ对肝纤维化及早期肝硬化的诊断价值较大,ＰＣⅢ更倾向于反映活动性肝纤维化。     

健康儿童膝关节屈伸工作时的生物力学规律

背景:在等速肌力测试的人群中,关于儿童的等速肌力测试研究报道较少。目的:通过对儿童膝关节屈伸肌力情况的测试,探讨儿童膝关节屈伸工作时的生物力学规律。设计、时间及地点:对比观察,于2006-10-12在河北师范大学体育学院实验室进行测试。对象:志愿受试的健康儿童选自石家庄市部分小学10岁左右的学生66名,男31名,女35名。方法:运用澳大利亚Kylingk公司生产的"Kinitech"等速肌力测试系统进行等速肌力测试,采用60,120,240(°)/s3种速度测试儿童膝关节屈伸肌力。主要观察指标:男女儿童屈伸肌峰力矩值比较和最大功率比较。结果:①3种不同测试速度中,男女儿童膝关节屈伸肌峰力矩值呈现随测试速度的增加而下降,最大功率随测试速度的增加而增加的趋势。儿童左侧峰力矩值大于右侧。③男女儿童各自屈伸肌峰力矩值均值比值F/E>0.7,并且随测试速度的增加而增大。④在60,120(°)/s的测试中,膝关节屈肌和伸肌峰力矩值性别差异无显著性意义(P>0.05),240(°)/s测试中左右侧屈肌峰力矩值性别差异具有显著性意义(P<0.01,P<0.05),男生左侧伸肌峰力矩值大于女生(P<0.05)。结论:儿童在不同的测试速度中,峰力矩及最大功率表现出的变化趋势与成人一致。提示儿童早期参加体育活动时应注意发展屈肌力量,使屈伸肌力量平衡发展。     

急性坏死性胰腺炎胰腺组织中褪黑激素受体表达及褪黑激素干预作用

目的 探讨急性坏死性胰腺炎(ANP)胰腺组织中褪黑激素受体MT1表达及褪黑激素(MT)干预作用.方法 54只SD大鼠随机分为假手术组(SO组)、急性坏死性胰腺炎组(ANP组)、MT干预组(MT组),每组18只.应用胰胆管逆行注射牛磺胆酸钠的方法诱导ANP模型,MT组于诱导模型前30 min腹腔注射MT.术后4 h、8 h和12 h分批处死大鼠(每个时间点6只),以免疫组化和实时定量PCR分别检测胰腺组织中MT1蛋白及MT1 mRNA的表达;检测血清淀粉酶、胰腺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)及TNFα的含量,并行胰腺病理检查.结果 (1)ANP组胰腺病理损伤呈进行性加重,MT组胰腺病理损伤较ANP组明显减轻(P＜0.05).(2)淀粉酶、MDA、TNFα水平在ANP组较S0组明显增高(P＜0.05或P＜0.01),而MT组较相应时间点ANP组显著降低[12 h,(2348.00±278.90)U/L比(3194.83±538.10)U/L,(2.255±0.472)μmol/L比(2.960±0.722)μmol/L,(102.929±29.399)ng/L比(378.544±183.454)ng/L,P＜0.05].SOD水平在ANP组各时点较SO组显著降低(P＜0.01),而MT组较ANP组显著升高[12 h,(11.448±1.594)U/L比(8.427±1.950)U/L,P＜0.05].(3)与SO组相比,MT1蛋白及MT1 mRNA表达在ANP组胰腺组织中明显下降(P＜0.05),且随ANP病变加重表达减弱,而MT组表达较ANP组明显增多.结论 MT干预可提高SOD活力,降低MDA、TNFα水平,故能显著减轻ANP时胰腺病理损伤,MT1在ANP发病机制中可能具有重要作用,MT对ANP中的干预作用可能与上调胰腺组织中MT1的表达有关.     

Thermo-Viscoelastic Response of 3D Braided Composites Based on a Novel FsMsFE Method

A homogenization-based five-step multi-scale finite element (FsMsFE) simulation framework is developed to describe the time-temperature-dependent viscoelastic behavior of 3D braided four-directional composites. The current analysis was performed via three-scale finite element models, the fiber/matrix (microscopic) representative unit cell (RUC) model, the yarn/matrix (mesoscopic) representative unit cell model, and the macroscopic solid model with homogeneous property. Coupling the time-temperature equivalence principle, multi-phase finite element approach, Laplace transformation and Prony series fitting technology, the character of the stress relaxation behaviors at three scales subject to variation in temperature is investigated, and the equivalent time-dependent thermal expansion coefficients (TTEC), the equivalent time-dependent thermal relaxation modulus (TTRM) under micro-scale and meso-scale were predicted. Furthermore, the impacts of temperature, structural parameters and relaxation time on the time-dependent thermo-viscoelastic properties of 3D braided four-directional composites were studied.     

Partial trisomy 16q and partial monosomy 7p of a fetus derivated from paternal balanced translocation: A case report

Introduction:Subchromosomal deletions and duplications could currently be detected by noninvasive preliminary screening (NIPS). However, NIPS is a screening test that requires further diagnosis. Here we report a fetus with an autosomal abnormality revealed by NIPS and conventional karyotype combined with copy number variations sequencing (CNV-seq) confirmed the fetus with an unbalanced translocation.Patient concern:This was the fourth pregnancy of a 30-year-old woman who underwent 2 spontaneous abortions and gave birth to a child with a normal phenotype. The woman and her husband were healthy and nonconsanguineous. NIPS indicated a repeat of about 19-Mb fragment at the region of 16q22.1-q22.4 at 17-week gestation.Diagnoses:The combination of traditional karyotype and CNV-seq could better locate the abnormal chromosomal region and further identify the source of fetal chromosomal abnormalities. Simultaneously, we evaluated the fetal morphology by ultrasound examination. The karyotype of the fetus was 46,XX,der(7)t(7;16)(p22;q23) and CNV-seq results showed an approximately 20.96-Mb duplication in 16q22.1-q24.3 (69200001-90160000) and an approximately 3.86-Mb deletion in 7p22.3-p22.2 (40001-3900000). Prenatal ultrasound revealed the fetal micrognathia. The paternal karyotype was 46,XY, t (7;16) (p22;q23), while the maternal was normal. The fetus inherited an abnormal chromosome 7 from its father.Interventions:No treatment for the fetus.Outcomes:Pregnancy was terminated.Conclusions:To our knowledge, the occurrence of de novo partial trisomy 16q (16q22.1-qter) and partial monosomy 7p (7p22.2-pter) has not previously been reported up to now. Here, we present the perinatal findings of such a case and a review of the literatures. CNV-seq combined with karyotype is a useful tool for chromosomal abnormalities indicated by NIPS.     

自体外周血干细胞移植治疗乙型肝炎肝硬化患者疗效及安全性分析

目的 临床可用于治疗失代偿期乙型肝炎肝硬化患者的有效措施有限,且效果多不理想。干细胞移植是一个理想的选择。本研究的目的是评估自体外周血干细胞移植（APBSCT）治疗乙型肝炎肝硬化患者的疗效和安全性。方法 在100例乙型肝炎肝硬化患者中,对照组50例接受常规治疗,在另50例,先接受重组人粒细胞集落刺激因子（rhG-CSF）注射动员,然后分离和采集外周血干细胞,并通过介入方法注入肝固有动脉。观察6 m。结果 在干细胞移植后1 m,移植组和对照组患者血清TBIL水平分别为（34.5±17.2） μmol/L和（59.3±12.8） μmol/L （P<0.05）,ALB分别为（35.3±3.2） g/L和（30.2±1.5） g/L（P<0.05）,差异显著;术后6 m,血清ALB水平分别为（30.6±4.4） g/L和（27.6±1.9） g/L（P<0.05）, Child-Pugh评分分别为（8.4±2.1）分和（9.7±3.3）分（P<0.05）,差异显著。结论 应用APBSCT治疗乙型肝炎肝硬化患者安全,近期有效,但对预后的影响还需要观察。     

Limited number of immunoglobulin VH regions expressed in the mutant rabbit “Alicia”

A unique feature of rabbit Ig is the presence of V_H region allotypic specificities. In normal rabbits, more than 80% of circulating immunoglobulin molecules bear theV_Ha allotypic specificities, a1, a2 or a3; the remaining 10% to 20% of immunoglobulin molecules lack V_Ha allotypic specificities and are designated V_Ha^?. A mutant rabbit designated Alicia, in contrast, has predominantly serumimmunoglobulin molecules that lack the V_Ha allotypic specificities (Kelus and Weiss, Proc. Natl. Acad. Sci. USA 1986. 83: 4883). To study the nature and molecular complexity of V_Ha^? molecules, we cloned and determined the nucleotide sequence of seven cDNA prepared from splenic RNA of an Alicia rabbit. Six ofthe clones appeared to encode V_Ha^? molecules; the framework regions encoded by these clones were remarkably similar to each other, each having an unusual insertion of four amino acids at position 10. This insertion of four amino acids has beenseen in only 2 of 54 sequenced rabbit V_H genes. The similarity of the sequences of the six V_Ha^? clones to each other and their dissimilarity to most other V_H genes leads us to suggest that the V_Ha^? molecules in Alicia rabbits are derived predominantly from one or a small number of very similar V_H genes. Such preferential utilization of a small number of V_H genes may explain the allelic inheritance of V_H allotypes.     

肝力注射液逆转人肝癌细胞获得性多药耐药的体外研究

目的研究肝力注射液体外逆转人肝癌细胞5-Fu获得性多药耐药的作用。方法MTT法检测肝力注射液对人肝癌BEL-7402/5-Fu耐药细胞株的增殖抑制率及对5-Fu获得性多药耐药的逆转指数,倒置显微镜及Wright-Giemsa染色观察细胞形态学改变,流式细胞术检测细胞内阿霉素荧光强度。结果人肝癌BEL-7402/5-Fu细胞株对临床常用化疗药物显示出不同程度的交叉抗药性,肝力注射液(以苦参碱计)在0.230、0.115、0.058mg/mL3个质量浓度对人肝癌BEL-7402耐药细胞株72h的增殖抑制率分别为(9.25±2.38)%、(8.46±1.90)%、(4.23±2.05)%,对5-Fu的逆转指数分别为5.50、3.83、2.25,肝力注射液在0.230mg/mL质量浓度预处理细胞2h能提高细胞内阿霉素的量约38.61%。结论肝力注射液在体外具有逆转人肝癌细胞5-Fu获得性多药耐药的作用。     

五虎汤联合穴位贴敷对支气管哮喘患者气道重塑及MMP-9因子的影响

目的:探讨五虎汤联合穴位贴敷对支气管哮喘患者的疗效及对气道重塑、MMP-9因子的影响。方法:选取2017年1—12月新疆医科大学附属中医医院收治的支气管哮喘患者80例作为研究对象。按照随机数字表法随机分为对照组和观察组,每组40例。对照组采用布地奈德福莫特罗粉吸入剂联合硫酸沙丁胺醇治疗,观察组在对照组基础上给予中药五虎汤联合穴位贴敷治疗。比较2组患者治疗前、治疗后肺功能(FEV1%、PEFR、MMEF)、支气管黏膜基底膜厚度(TRBM)、支气管上皮黏膜肌纤维母细胞计数、MMP-9及TGF-β1水平变化情况及临床疗效。结果:2组治疗前FEV1%、PEFR、MMEF、TRBM、支气管上皮黏膜肌纤维母细胞计数、MMP-9及TGF-β_1水平比较,差异无统计学意义(P 0. 05);治疗后2组FEV1%、PEFR及MMEF水平均显著升高,支气管上皮黏膜肌纤维母细胞计数、TRBM、MMP-9及TGF-β_1水平显著降低,差异有统计学意义(P 0. 05);观察组FEV1%、PEFR及MMEF水平均显著高于对照组,支气管上皮黏膜肌纤维母细胞计数、TRBM、MMP-9及TGF-β_1水平显著低于对照组,差异有统计学意义(P 0. 05)。观察组总有效率为95. 00%(38/40),对照组总有效率为90. 00%(36/40),2组比较差异无统计学意义(P 0. 05)。结论:五虎汤联合穴位贴敷可显著改善支气管哮喘患者肺功能指标,降低患者支气管上皮黏膜肌纤维母细胞计数、TRBM、MMP-9及TGF-β_1水平,疗效显著,且用药安全无不良反应,在临床支气管哮喘的治疗中具有重要的应用价值。     

响应面优化酶法提取石榴皮多糖及其抗氧化活性研究

目的 采用响应面法优化石榴皮多糖的酶法提取工艺,并对石榴皮多糖体外抗氧化活性进行研究,为药物制剂和功能性食品寻找新的生物成分。方法 采用RSM Box-Behnken设计法,考察酶解时间、液料比、加酶量对石榴皮多糖提取率的影响。用酶标仪测定DPPH自由基清除作用、羟自由基清除作用、超氧阴离子自由基清除活性和还原力测定。结果 最佳提取条件为：酶解温度为55℃,pH为5,酶解时间为88 min,液料比为22:1mL/g,加酶量为0.93%。在最佳提取条件下,石榴皮多糖得率为（22.31±0.07）%,与Box-Behnken设计模型的预测值22.35%很好地吻合。石榴皮多糖对DPPH（1,1-二苯基-2-吡啶基肼）自由基清除、羟自由基清除、超氧阴离子自由基清除和还原能力有明显的抗氧化作用。结论 建议采用优化的酶解辅助方法提取石榴皮多糖,该法制得的石榴皮多糖可作为一种良好的抗氧化剂。