促结缔组织增生型成釉细胞瘤细胞的体外培养与鉴定

目的：观察体外培养的促结缔组织增生型成釉细胞瘤细胞的生长特点。方法：对促结缔组织增生型成釉细胞瘤细胞用酶消化法进行体外培养，免疫组化证实其细胞来源，以倒置显微镜观察细胞形态及生长情况．并用流式细胞仪分析细胞周期，MTT法绘制细胞生长曲线。结果：促结缔组织增生型成釉细胞瘤为上皮来源，细胞在体外培养过程中包含2种形态的细胞，与实性成釉细胞瘤细胞在体外培养相比，存在较多梭形细胞，细胞生长缓慢，培养的各代细胞未见异倍体。结论：促结缔组织增生型成釉细胞瘤细胞在体外培养过程中包含较多的梭形细胞．可能与其较多的细胞间质有关。     

骨性Ⅲ类错（牙合）正畸-外科联合治疗后牙齿长期稳定性研究

目的　探讨口腔正畸 正颌外科联合治疗前后不同阶段牙齿的变化和术后的稳定性。方法　选取 31例在北京大学口腔医学院进行口腔正畸 正颌外科联合治疗的骨性安氏III类错患者 ,对其不同阶段X线头影测量片进行研究。结果　牙齿在术前正畸阶段有明显的去代偿 ,下切牙平均唇倾 7 0 8°。在术中 ,上切牙随颌骨的旋转有直立 ,下切牙有后移。术后 ,上切牙有唇向复发 ,下切牙相对于基骨稳定。术后牙齿保持良好的咬合 ,而且主要变化发生在术后正畸阶段。结论　III类错患者在术前正畸阶段牙齿明显去代偿 ,术后上前牙稍有复发 ,下前牙相对保持稳定     

阿替卡因与利多卡因在活髓牙预备时的疗效和对心血管系统的影响的比较

目的:比较阿替卡因(碧兰麻)与利多卡因在活髓牙预备时的麻醉效果和对心血管系统的影响。方法:随机数表将93例患者的138颗牙编号分组,以VAS(Visual Analogue Scale)标尺法由患者对疼痛程度进行打分;口腔医生评价麻醉效果,统计麻醉完全、良好、有效和失败的比例;观测患者在麻药使用前、即刻、3分钟、5分钟的血压和心跳。结果:两组的VAS评分存在显著性差异(P<0.01),麻醉完全、良好、有效和失败的比例也存在显著性差异(P<0.01)。两种麻药对血压心率均无显著性影响。结论:阿替卡因是一种有效的、安全的麻醉剂。     

导管消融联合左心耳封堵一站式治疗心房颤动相关用药的研究进展

导管消融联合左心耳封堵一站式手术是治疗心房颤动的热点之一,除了手术操作,相关用药也是保证手术成功、降低并发症的重要环节.该文结合国内外文献,就一站式治疗过程中涉及的药物治疗方案,如抗凝或抗血小板药物、质子泵抑制剂、抗菌药物、麻醉药物、抗心律失常药物的选择、使用时机、疗程等进行综述,供临床参考与借鉴.     

双侧卵巢肿瘤生育力保存的1例报道并文献复习

目的 探讨年轻卵巢肿瘤患者保留生育力的相关诊疗方案.方法 回顾性剖析该院1例双侧卵巢肿瘤患者的临床资料及诊疗过程,并复习国内外相关文献.结果 患者术后定期随访肿瘤标志物及影像学检查,均未见明显异常,现已获得满意的妊娠结局(2/3),其并未借助任何辅助生殖技术分别于2015、2018年于该院产科顺产两活婴.结论 年轻的卵巢肿瘤患者除了行保留生育力治疗,还可采取相应措施来保护患者的卵巢功能.     

双侧卵巢肿瘤生育力保存的1例报道并文献复习

目的 探讨年轻卵巢肿瘤患者保留生育力的相关诊疗方案.方法 回顾性剖析该院1例双侧卵巢肿瘤患者的临床资料及诊疗过程,并复习国内外相关文献.结果 患者术后定期随访肿瘤标志物及影像学检查,均未见明显异常,现已获得满意的妊娠结局(2/3),其并未借助任何辅助生殖技术分别于2015、2018年于该院产科顺产两活婴.结论 年轻的卵巢肿瘤患者除了行保留生育力治疗,还可采取相应措施来保护患者的卵巢功能.     

肺肿瘤性磨玻璃结节平均CT值测量相关技术的影响因素研究

目的 分析CT扫描剂量、重建算法、层面选择及胸部不同位置对纯磨玻璃肺结节(pGGN)平均CT值测量偏差度的影响,评价影响肺肿瘤性磨玻璃结节(GGN)平均CT值测量的因素.方法 选用CT值为-650 HU的不同直径球形模拟肺pGGN,应用东芝(佳能)64排螺旋CT,设置4种剂量等级自动管电流调制技术扫描,3种三维权重自适应迭代重建剂量降低(AIDR-3D)算法重建,测量1 mm薄层图像肺结节平均CT值,计算偏差度.结果 低剂量模式扫描时,小于10 mm的球形pGGN平均CT值的偏差度较大.3种AIDR算法重建下,相同直径球形pGGN平均CT值偏差度差异较小;pGGN直径越小,球形pGGN平均CT值偏差度越大.在球形pGGN中心层面测量CT值,平均CT值的偏差度最小;偏离中心层面越远,偏差度越大.胸部3个位置的pGGN平均CT值偏差度均不同,肺实质内平均CT值偏差度最小.结论 除重建算法,CT扫描剂量、测量层面选择及结节所在胸部位置对于pGGN平均CT值测量的精准度均可造成影响.     

纤维桩和黏结剂的弹性模量对纤维桩修复应力的影响

目的:研究纤维桩和黏结剂的弹性模量对纤维桩修复应力的影响,为临床上合理选择纤维桩和黏结剂提供实验依据.方法:建立下颌第二前磨牙纤维桩修复的三维有限元模型,设定纤维桩弹性模量(Ep)在15～50 GPa之间、黏结剂弹性模量(Ea)在2～20 GPa之间变化,以牙体组织和黏结剂层的Von Mises应力峰值为观察指标,应用Ansys Workbench DesignXplorer分析软件,研究纤维桩和黏结剂的弹性模量对纤维桩修复应力的影响.结果:一定载荷下,牙体组织的Von Mises应力峰值随着Ep和Ea增大而减小,减小幅度为4.45%;黏结剂层的Von Mises应力峰值随着Ep和Ea增大而增大,增大幅度为158.68%;与Ep相比,黏结剂层Von Mises应力峰值改变对变量Ea的敏感度更高.结论:当纤维桩弹性模量在15～50 GPa之间、黏结剂弹性模量在2～20 GPa之间变化时,纤维桩和黏结剂的弹性模量改变对牙体组织应力无明显影响;黏结剂层应力随着纤维桩和黏结剂的弹性模量的增大而增大;黏结剂的弹性模量改变对于黏结剂层应力的影响更大;选择低弹性模量的纤维桩和黏结剂对保护纤维桩黏结界面是有利的.     

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study

Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women.     

Comparative Study of the Genetic Diversity,Antimicrobial Resistance,and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals

Objective This study was performed to compare the genetic diversity, virulence, and antimicrobial resistance of Aeromonas strains isolated from patients and healthy individuals. Methods A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma'anshan City, Anhui Province. Their taxonomy was investigated using concatenated gyrB-cpn60 sequences, and their resistance to 12 antibiotics was evaluated. The pathogenicity of these strains was examined through beta-hemolysis, protease activity, and virulence gene assays. Results The 57 Aeromonas strains were divided into 55 sequence types. Of these types, 21 were novel, suggesting that their genetic diversity was high. These Aeromonas isolates could be divided into 7 species, and the positive rates of beta-hemolysis and protease activity were 49.1％ and 73.7％, respectively. The detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy individuals. Among the four most common Aeromonas strains, A. dhakensis had the highest detection rate of virulence genes. The multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy individuals. Conclusions The taxonomy, virulence properties, and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.