牙片中骨量与灰度的关系及灰阶的改进

本文对较小厚度范围内颌骨、金属铝在X片上的灰度水平与厚度变化关系进行了探讨,提出在数字减影牙片中以金属铝制成均匀楔形灰阶,代替传统金箔制成的台阶式灰阶.     

HBV DNA及HBxDNA亚片段在肝癌细胞内的整合及作用

为探讨ＨＢＶＤＮＡ及其亚基因片段ＨＢｘＤＮ在肝细胞肝癌（ＨＣＣ）中整合特点用机制，制备了地高辛标记的３．２ｋｂＨＢＶＤＮＡ全基因及０．５９ｋｂＨＢＶＤＮＡ／ＢａｍＨＩ，ＢｇｌⅡＢＨｘＤＮＡ亚基因探针。点杂交、原位杂交及Ｓｏｕｔｈｅｒｎ杂交检测ＨＢＶＤＮＡ的存在情况，筛选ＨＢＶＤＮＡ纯整合标本，以ＨＢｘＤＮＡ探针检测ＨＢｘＤＮＡ在ＨＣＣ染色体的整合。结果显示ＨＢＶＤＮＡ在肝细胞内以核型为主（７０％）     

慢性肝病患者血清和腹水肿瘤坏死因子—α检测的临床意义

目的：了解慢性肝病患者血清和腹水肿瘤坏死因子－α检测的临床意义。方法：采用双抗体夹心ＥＩＳＡ法，对２０例慢迁肝、２０例慢活肝和２９便肝硬化代偿期病人血清ＴＮＦ－α进行检测；对２１例肝硬化腹水无自发性腹膜炎（ＳＢＰ）者、２３例肝硬化腹水并ＳＢＰ者、２５例肝癌腹水者和２４例胃肠癌腹水者进行血清和腹水的ＴＮＦ－α检测。结果：血中ＴＮＦ－α水平在下列各组疾病呈逐渐上升（慢迁肝组〈慢活肝组和肝硬化代偿期组〉     

Normal delta-globin gene sequences in Sardinian nondeletional delta beta-thalassemia.

In order to clarify the reasons for the reduced Hb A2 levels in Sardinian delta beta-thalassemia, we characterized, both by cloning and sequence analysis and by direct sequencing of amplified DNA, the delta-globin gene from an individual of Sardinian descent who is a compound heterozygote for the beta zero-thalassemia codon 39 (C-->T) nonsense mutation and the Sardinian delta beta-thalassemia [codon 39(C-->T)/-196(C-->T)A gamma]. The analysis of the delta-globin gene from the delta beta-thalassemia chromosome revealed an entirely normal sequence. The defective function of the delta-globin gene in this determinant is thus likely related to a suppressive effect of the in cis nondeletional high persistence of fetal hemoglobin mutation of the A gamma gene, probably resulting from an increased capability of the relative promoter to interact with the locus control region.     

Correlation between serological and mucosal inflammatory responses to Helicobacter pylori.

In 82 patients who underwent gastroduodenoscopy, acute and chronic gastric mucosal inflammation was scored for severity, and systemic humoral immune responses to Helicobacter pylori antigens were assessed by enzyme-linked immunosorbent assays. On the basis of culture, gastric histology, and serologic evaluation, 33 patients were classified as H. pylori infected and 36 were classified as uninfected. Thirteen patients had negative cultures and stains but were seropositive and were analyzed separately from the other two groups. Specific serum immunoglobulin G (IgG) subclass responses to H. pylori whole-cell antigens and specific IgG responses to the 54-kDa heat shock protein homolog (Hp54K) and vacuolating cytotoxin were significantly greater in infected than in uninfected patients as were specific IgA responses to whole-cell antigens and cytotoxin (P < 0.001). Among the H. pylori-infected persons, serum IgG responses to Hp54K and to the vacuolating cytotoxin were correlated with acute mucosal inflammatory scores. In contrast, serum IgA responses to whole-cell sonicate and to vacuolating cytotoxin were inversely related to chronic inflammatory scores. By multivariant regression analysis, only specific serum IgG responses to Hp54K correlated with severity of inflammation (both acute and chronic; P < 0.001); these responses may be markers of inflammation or these antibodies could play a direct role in the pathogenesis of H. pylori-induced inflammation.     

7种蛇类药材的等电点鉴别研究

用改进的蛋白质等电点法对7种蛇类药材进行了鉴别研究。结果表明不同种类的蛇,等电点有所不同。重现性好(CV<0.5),其等电点均值的差别(P<0.01)有非常显著的意义,可以作为去皮或粉末状蛇类药材鉴别的依据之一。     

聚合酶链反应（PCR）对单纯疱疹病毒性脑炎的临床诊断价值

应用聚合酶链反应（ＰＣＲ）检测技术对１１８例颅内感染性疾病患者及３７例无神神经系统疾病患者脑脊液（ＣＳＦ）中的单纯疱疹病毒ＤＮＡ（ＨＳＶ－ＤＮＡ）进行了检测及分型。结果提示：“散发性脑炎”组的阳性率为３８．４６％（２０／５２），细菌、真菌性脑膜炎、其它病毒性脑炎组以及无神经系统疾病组均为阴性。作者认为：①本检测是目前单纯疹病毒性脑炎（ＨＳＥ）较为简便而准确的早期诊断方法之一；②ＨＳＶ分型检测，对病原诊断更具有全面性；③两型单纯疱疹病毒均可引起ＨＳＥ。     

大狼毒三萜类化学成分的研究

自大戟科(Euphorbiaceae)植物大狼毒(Euphorbia nematocypha Hand—Mazz)根的乙醇提取物的苯溶解部分,经20%AgNO₃硅胶层析,分离得到七个三萜类成分。根据光谱(IR,EIMS,¹H—NMR和¹³C—NMR)和化学方法,确定其中一个化合物为新化合物,命名为大狼毒醇(nematocyphol,Ⅳa),其它化合物为已知物:印度荆芥醇乙酸酯(nepehinol acetate Ⅰ),日尔曼醇乙酸酯(germanicol acetate Ⅱ),大戟醇(euphol,Ⅲ),蒲公英醇(taraxasterol,Ⅴa),24-亚甲基环阿尔廷醇(24-methylenecycloartanol,Ⅴa)和印度荆芥醇(nepehinol,Ⅶa)。这些化合物均为首次从大狼毒中得到。     

人自然杀伤细胞抑制性受体P58.2基因克隆与鉴定

目的：P58．2基因克隆与鉴定。方法：采用RT—PCR技术，从正常人外周血单个核细胞中扩增自然杀伤细胞抑制性受体P58．2基因全长cDNA，经酶切后构建重组克隆载体，双酶切和测序鉴定。结果：RT—PCR获得了预期的扩增产物P58．2全长cDNA，成功构建了pSPORT1-P58．2重组克隆载体，酶切、酶谱分析与预期结果相符。DNA测序结果与GenBank登记的人P58．2cDNA全长碱基序列一致。结论：pSPORT1-P58．2重组克隆载体构建成功；P58．2基因序列与文献报道一致，为下一步构建表达载体和基因转染等工作打下良好的基础。     

链球菌蛋白G应用于ELISA的实验研究

对酶标记链球菌蛋白Ｇ（ＳＰＧ）在ＥＬＩＳＡ的应用条件进行了研究并与ＳＰＡ作了系统比较。ＳＰＧ与ＩｇＧ结合受温度影响，３７℃结合活性高于室温和４℃；反应时间定为２ｈ反应溶液ｐＨ值升高，ＳＰＧ的结合力亦增强。在同等条件下与酶标记ＳＰＡ进行比较，结果表明ＳＰＧ对小鼠单克隆抗体、羊抗人、兔抗ＩＦＮ－γ和驴抗兔Ｉｇｇ以及牛、大鼠和人ＩｇＧ的结合力均明显高于ＳＰＡ。ＳＰＧ用于大鼠血清抗ＩＦＮ－γ抗体的检测，其阳性率和抗体滴度均大大高于ＳＰＡ。因此，ＳＰＧ作为一种广谱的、高亲和力的ＩｇＧ结合物，是应用于ＥＬＩＳＡ的理想的替代二抗。