Glandular cystic cystitis

Cystic-glandular cystitis is considered as part of the urothelial pre-neoplastic proliferative abnormalities. This group includes atypical hyperplasia. Von Brunn's nidus, and cystitis cystica. They are a consequence of the changes experienced at the urothelium level in response to inflammation, irritation or carcinogens. Diagnosis is mainly based in the pathoanatomical study of the biopsy obtained following endoscopic resection. The signs and symptoms it presents are varied and show a clear relationship to distribution and extension of cysts. Treatment is based in the removal of irritative factors. Cystectomy with urinary by-pass may be necessary if required by clinical evolution.     

载体介导的RNA干扰技术抑制肝癌耐药细胞MDR1表达的研究

目的 :构建含多药耐药基因MDR1的短发夹状RNA(shorthairpinRNA ,shRNA)表达质粒 ,观察对肝癌耐药细胞Bel 740 2 /R的MDR1mRNA的抑制作用。方法 :利用分子克隆技术 ,将含MDR1的双链DNA ,与经双酶切后的载体PGE 1连接 ,构建pshRNA MDR1重组质粒 ,在脂质体的介导下转染肝癌耐药细胞株Bel 740 2 /R ;RT PCR分析MDR1mRNA的表达 ,MTT法检测细胞对药物的敏感性 ,流式细胞仪检测细胞内罗丹明 12 3 (Rh12 3 )的潴留和P gp的表达。结果 :PCR和DNA测序证实了表达质粒构建成功 ,并能明显地抑制MDR1mRNA的表达 ;对盐酸表柔比星和顺铂的半数抑制浓度IC50明显降低 ,P <0 0 5 ;P gp的表达阳性率降低了 5 7 3 % ;细胞内Rh12 3的浓度显著增高 ,P <0 0 5。结论 :构建的pshRNA MDR1表达质粒能有效地降低肝癌耐药细胞MDR1mRNA和P gp的表达。     

人类α型叶酸受体基因的克隆及其真核表达载体的构建

目的 探讨如何以人类α型叶酸受体(FOLR-1)基因为基础构建核酸疫苗。方法 应用RT-PCR技术，从人类卵巢癌细胞系-SKOV3细胞中扩增FOLR-1基因，插入克隆载体pGEM-T Easy，经DNA自动测序仪测序证实后，以亚克隆法构建于真核表达载体pcDNA3．1( )，并使用限制性内切酶酶切鉴定。结果 从卵巢癌细胞系SKOV3中成功扩增出FOLR-1基因，并克隆人pcDNA3．1( )载体。结论 成功构建FOLR-1的重组克隆及真核表达载体，为今后利用FOLR-1进行卵巢上皮性肿瘤的免疫及导向治疗研究打下了基础。     

Pulmonary tuberculosis in five young infants with nursery exposure: clinical, radiographic and CT findings

Clinical, radiographic (n = 5) and CT findings (n = 4) of five Korean infants ranging in age from 2 to 3 months with confirmed tuberculosis were retrospectively analysed. All of the patients were symptomatic, anergic to tuberculin, and had a positive culture of Myobacterium tuberculosis in gastric aspirates. The probable source of infection was the hospital in which they were born. CT scans demonstrated hilar and mediastinal lymph node enlargement with central low attenuation and peripheral enhancement in all cases. CT may be useful in diagnosis by demonstrating characteristic adenopathy and disseminated disease in young infants. Received: 2 September 1997 Accepted: 23 April 1998     

腺病毒介导的大肠杆菌CD自杀基因体内外对肿瘤杀伤作用的研究

编委会 Editorial Board名誉主编 Honorary Editor-in-chief吴孟超 Wu Meng chao(Second Military Medical University,Shanghai 200433学术顾问 Academic Advisers巴德年 Ba Denian(Chinese Academy of Medical Sciences,Beijing 100730)刘新垣 Liu Xinyuan(Shanghai Institute of Biochemistry,Chinese Academy of Sciences,Shanghai 200031)吴旻 WuMin(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)汤钊猷 Tang Zhaoyou(Shanghai Medical University,Shanghai 200032)主编 Editor-in-chief张友会 Zhang Youhui(Cancer Institute,Chinese Academy of Medical Sciences,Beijing,100021)副主编 Associate Editor-in-chief崔正言 Cul Zhenyan(Department of Immunology,Shandong Academy of Medical Sciences,Jinan 250001)钱振超 Qian Zhenchao(Department of Patho-physiology,Dalian Medical University,Dalian 116027)何球藻 He Qiuzao(Department of Immunology,Shanghai Medical University,Shanghai 200032)董志伟 Dong Zhiwei(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)常务副主编 Managing Editor-in-chief     

T细胞及相关免疫分子在淋巴细胞趋化因子增强肿瘤自杀基因疗法中的作用

本室以往的研究表明,用腺病毒作为载体将大肠杆菌胞嘧啶脱氨酶(CD)基因与小鼠淋巴细胞趋化因子(Itn)基因联合体内转染,具有显著的抗肿瘤效应.本文对其免疫机理进行了进一步研究,发现CT26结肠腺癌细胞体外经过CD/Ltn基因转染并给予前体药物5-FC后,CT26结肠腺癌细胞表达CD80和CD54分子明显增加,提示经CD自杀基因和Ltn基因联转移后,肿瘤细胞免疫原性增加.荷瘤小鼠体内经联合治疗后,小鼠脾细胞分泌IL-2和IFN-γ明显增加.体内用抗CD4、CD8抗体阻断实验研究结果的表明,联合应用自杀基因和Ltn基因治疗主要通过诱导CD8~ T细胞发挥抗肿瘤作用.     

经胸联合后正中径路切除后纵隔哑铃状神经源性肿瘤

目的探讨后纵隔哑铃状神经源性肿瘤的切除方法.方法1994年6月～2003年5月经胸联合后正中径路切除后纵隔哑铃状神经源性肿瘤4例,其中神经纤维瘤3例,神经鞘瘤1例.全部病人均选择经胸联合后正中径路切除肿瘤.结果4例肿瘤均全部切除,症状均有不同程度改善,术后病人均能正常生活,未见肿瘤复发.结论经胸联合后正中径路一期切除后纵隔哑铃状神经源性肿瘤是安全可靠的手术方式.     

复合诱变筛选avermectins高产菌株

由Streptomyces avermitilis初始菌株经过自然分离可以得到三种不同类型的菌株，即灰色粉末型、白色和光秃型。其中灰色粉末型产素水平最高，摇瓶发酵效价达到6835．1μg／ml，B1组分达到56．3％。经过紫外线和亚硝基胍对初始菌株的孢子的诱变及对其原生质体的诱变再生。并且经过异亮氨酸和链霉素的定向筛选，得到的高产菌株摇瓶发酵效价达到8542．9μg／ml，B。组分达到64．9％；同时通过对高产菌株进行传代培养检验其传代稳定性，结果表明，传代3代后，菌株开始退化，并且其发酵效价有比较显著的下降。     

利用均匀实验优化利福霉素SV发酵培养基氮源配比

应用均匀设计的方法对利福霉素SV发酵培养基中的氮源配比进行优化。研究了氮源中各成分对发酵效价的影响。确定各成分的最佳配比并用实验验证，使摇瓶发酵效价提高了10％以上。并对利福霉素SV产生菌在新、老培养基中的代谢特性进行了比较。     

卡巴胆碱对烧伤延迟复苏大鼠免疫功能的影响

Objective To explore the changing trend of Ia on monocyte, lymphocyte apoptosis rate, TNF-α and IL-6 in abdominal aorta of burned rats with delayed resuscitation and the influence of application of carbachol on them. Methods Adult male Wistar rats were randomly divided into normal control group(n =8), scald group(n =48) and scald with carbachol treatment group(n =48). In latter two groups, rats were inflicted with 30% total body surface area (TBSA) full-thickness scald and delayed fluid resuscitation. All scald rats were sacrificed at the 6th hours or 1st, 2nd, 3rd, 7th, 14th day after scald, with 8 rats at each time point. Expression of Ia antigen on monocyte and lymphocyte apoptosis rate were determined by direct immunofluorescence on a flow cytometer, and TNF-α and IL-6 was measured by ELISA. Results Expression of la on monocyte was obviously lower than that of controls. The lowest levels were recorded on the 6th hours and 1st day after scald. Subsequently, Ia was elevated gradually, but still lower than that of normal rats(P <0. 01). After administration of carbachol, Ia expression was obviously promoted, compared with the simple scald group (P <0. 01). Lymphocyte apoptosis rate, TNF-α and IL-6 was higher than that of controls(P <0. 01). After administration of cavachol, , lymphocyte apoptosis rate and TNF-α and IL-6 was obviously down-regulated on the 6th hours, 1st day, 2nd day and 3rd day after scald injury, compared with the simple scald group (P < 0. 01 or 0. 05). Conclusion After severe burn with delayed fluid resuscitation, there is a low la expression, high lymphocyte apoptosis rate and increased releasing of proinflammatory cytokine. Immune function was suppressed. Carbacho] could improve the immune function of scald rats with delayed fluid resuscitation.