肾盂输尿管癌细胞增殖与凋亡的相关性

目的探讨Ki-67和凋亡（Apoptosis）在肾盂输尿管癌中的表达及其与肿瘤细胞分级、病理分期及预后的关系。方法采用SABC法和TUNEL法对34例肾盂输尿管癌行Ki-67和凋亡检测。结果Ki-67和凋亡在肿瘤细胞分级间存在显著差异,Ki-67index：G2与G1比较P〈0.05,G3与G2比较P〈0.05,G3与G1比较P〈0.01;Apoptosis index：G2与G1比较P〈0.05,G3与G2比较P〈0.05,G3与G1比较P〈0.01。Ki-67和凋亡在肿瘤病理分期的T3与T1及T2与T1之间有显著差异,T2与T1比较P〈0.05,T3与T1比较P〈0.01,但T3与T2比较P〉0.05;Ki-67index以20%为界分为二组,Apoptosis index以3%为界分为二组,组间生存率有显著差异（P〈0.05）。Ki-67在肾盂输尿管癌中的表达与凋亡一致。结论肾盂输尿管癌细胞增殖与凋亡密切相关;凋亡可作为临床判断肾盂输尿管癌恶性程度、病理分期、细胞分级以及预后的一种客观量化指标。     

龙眼参提取物对耐药口腔上皮样癌细胞株KB／MIT及耐药乳腺癌细胞株MCF-7／ADR的逆转作用

目的：研究龙眼参提取物XCKB／MIT细胞及MCF-7／ADR细胞株的耐药逆转作用。方法：设计4个不同浓度的龙眼参提取物组，采用MTT方法．测定体外培养的阿霉素敏感株MCF-7及其耐药株MCF-7／ADR以及米托蒽醌敏感株KB及其耐药株KB／MIT的IC50。结果：MCF-7／ADR耐药株及KB／MIT耐药株在龙眼参提取物与阿霉素或米托蒽醌的联合用药中其IC50值分别接近于单独用约于敏感株MCF-7和KB的水平．而与单独用药于耐药株其IC50有显著性差异。结论：龙眼参提取物可以逆转MCF7／ADR和KB／MIT的耐药性。     

浆细胞病患者IL-4产生细胞数减少的意义及机制探讨

本文运用APAAP法检测了16名浆细胞患者外周血细胞在PHA刺激下产生IL-4的能力。发现浆细胞病组阳性细胞比率明显低于正常对照组(P<0.001),且阳性细胞比率与患者血清中多克隆IgM、IgA水平呈正相关(r=0.806和r=0.703)。在正常人外周血中加入CRP50mg/L,经PHA刺激后IL-4产生细胞也明显减少。作者假设,由于IL-6增高引起CRP等急性期相蛋白大量分泌,使IL-4产生受到明显抑制,从而使B细胞激活发生障碍是浆细胞病中多克隆免疫球蛋白合成抑制的主要原因。     

RESEARCH ON A REAL-TIME CONTROL SYSTEM OF LOW POWER DISSIPATION FOR CONTINUOUS CYCLIC PERITONEAL DIALYSIS(CCPD) CYCLER

FOREWORDPeritonealdialysisrepresentssoluteandfluidexchangemainlybetweenperitonealcapillarybloodanddialysissolutionintheperitonealcavity'CombinedwithhemodialySisandrenaltransplantation,peritonealdialysisisusedmainlyforthetreatmentofrenalfailure,particularlyforchronicfailure'In1976,Prof'Popovichdevelopedcontinuousambulatoryperitonealdialysis(CAPD)basingontheconceptofcontinuousequilibrationdialysis('1'Fromthenon,peritonealdialysisfounditsclinicaluse'Continuouscyclicperitonealdialysis(CCPD…     

青果微量元素的测定及其含量与功效的关系

用电感耦合等离子体发射光谱法(ICP—AES),同时测定了广州与潮州产青果中13种微量元素和宏量元素的含量,结果发现其含有多种人体必需和与乙型肝炎的发生发展有关的微量元素;初步讨论了它们与青果功效的关系。     

蝎毒素Ⅳ的中枢镇痛作用

从东亚钳蝎粗毒中分离纯化出蝎毒素Ⅳ，经套管注入侧脑室，用辐射热测痛和屈肌反射检测两种方法，观察蝎毒素Ⅳ的中枢镇痛作用，结果蝎毒索Ⅳ可以明显延长大鼠缩腿潜伏期，并可显著抑制Ｃ纤维诱发的屈肌反射。提示蝎毒素Ⅳ具有显著的中枢镇痛作用。     

医疗体系中外展社区服务的重要性

院外医护服务在香港医疗体系中起着重要作用。院外医护医疗工作者如社区护士、家庭服务志愿者和社会工作者来自于社区的助人网络。 院外医护人员的优点是: (1)延长医护服务的连续性;(2)缓解病人的心理压力;(2)监察病情;(4)推广健康教育知识。     

与眶下神经相关的初级传入纤维终末的溃变、非突触部位胞吐及突触联系

切断眶下神经的各组大鼠存活2～30天后分别杀死,于其三叉神经尾侧脊束核胶状质亚核内观察了一级传入纤维轴突终末的溃变过程.非突触部位胞吐及突触联系.结果发现:(1)眶下神经的跨节溃变、以突触小泡聚集、融合、空泡形成为主要特征,无微丝增生现象:(2)部分溃变终末内的线粒体明显肿胀变暗.呈球形改变:(3)大致密核心小泡的溃变时间远滞后于突触小泡.两者并不同步进行;(4)轴突终未在溃变过程中,其内的大致密核心小泡仍然进行非突触部位胞吐;(5)溃变纤维终末于胶状质内分别形成轴一树、轴一体、轴一轴三种类型的突触、并参与了突触复合体的形成.     

Discordant quantitative detection of putative biomarkers in nodal micrometastases of colorectal cancer: biological and clinical implications

AIMS: Nodal expression of the carcinoembryonic antigen (CEA), cytokeratin 20 (CK20), and guanylyl cyclase C (GCC) genes was measured in tandem in patients with colorectal cancer (CRC) to assess whether there would be sufficient agreement between these markers in their ability to detect micrometastasis to qualify one of them as a universal marker, and whether frozen and paraffin wax embedded tissues would yield similar results. METHODS: One hundred and seventy five frozen lymph nodes (FT) and 158 formalin fixed, paraffin wax embedded lymph nodes (PET) from 28 CRC cases were analysed using gene specific quantitative real time polymerase chain reaction, carried out on the LightCycler system with SYBR Green chemistry. RESULTS: There was significant disparity in positive detection of the three biomarkers in FT versus PET, with notable agreement achieved only for CEA (66.6%) in FT versus PET in Dukes' B disease, and between CK20 and GCC (44.6%) in FT, also in Dukes' B disease. One patient with full concordance in all three tumour markers with both tissue types suffered a relapse and died within two years of follow up. CONCLUSIONS: There was considerable discordance in the positive detection of the three tumour markers in both tissue types (FT versus PET). This brings into question whether using a single tumour marker to detect micrometastasis in one tissue type (FT or PET) is adequately representative, and challenges the concept of universal markers for molecular CRC metastatic detection. Multiple tumour markers would predict more accurately the metastatic potential of Dukes' B CRCs.     

TGF-β1 Null Mutation Leads to CD154 Upregulated Expression in Affected Tissues

The TGF-1(–/–) mouse is a murine model for systemic autoimmune disease. The aim of this study is to elucidate the immunological mechanism that leads to multifocal tissue inflammation and autoantibody production in TGF-1(–/–) mice. Heart, lung, liver, and salivary gland from TGF-1(–/–) were assessed for CD154 expression by RT-PCR and immunohistochemistry. Compared to wild-type littermates, CD154 expression was elevated in all tissues studied. Furthermore, IL-12 mRNA was expressed in the salivary gland and heart of TGF-1(–/–) mice and not in wild-type littermates. This suggests that the CD154 pathway is activated in these tissues. This shows that TGF-1 regulates CD154 expression leading to spontaneous IL-12 production and autoimmunity.