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61.
Wei Tseng Chen Tzung Lieh Yeh Venla Lehti Shu Hui Cheng Ching Lin Chu Kao Chin Chen 《Behavioral medicine (Washington, D.C.)》2013,39(2):49-53
Although the Dexamethasone Suppression Test (DST) plays an important role in psychosomatic research, confounding factors limit the sensitivity and specificity of the DST. The aim of this study was to investigate the relationship between the intensity of daily life stressors and DST results in healthy participants after controlling the confounding factors. The subjects of this study consisted of 75 healthy volunteers. The intensity of daily life events was assessed using the Taiwanese version of the Recent Life Change Questionnaire (RLCQ). Neuroticism was assessed using the Maudsley Personality Inventory (MPI). The Dexamethasone Suppression Test (DST) was also performed. The regression model showed that daily life events (RLCQ score) were correlated significantly with cortisol level on day 1 and D% only in women. This finding implies that daily life events should be considered as an independent variable in women in further studies when the DST is applied. 相似文献
62.
Xiao?Teng?Ching Yee?Ling?LauEmail author Mun?Yik?Fong Veeranoot?Nissapatorn 《Parasitology research》2013,112(3):1229-1236
Toxoplasma gondii infects all warm-blooded animals including humans, causing serious public health problems and great economic loss in the food industry. Commonly used serological tests involve preparation of whole Toxoplasma lysate antigens from tachyzoites which are costly and hazardous. An alternative method for better antigen production involving the prokaryotic expression system was therefore used in this study. Recombinant dense granular protein, GRA2, was successfully cloned, expressed, and purified in Escherichia coli, BL21 (DE3) pLysS. The potential of this purified antigen for diagnosis of human infections was evaluated through western blot analysis against 100 human serum samples. Results showed that the rGRA2 protein has 100 and 61.5 % sensitivity towards acute and chronic infection, respectively, in T. gondii-infected humans, indicating that this protein is useful in differentiating present and past infections. Therefore, it is suitable to be used as a sensitive and specific molecular marker for the serodiagnosis of Toxoplasma infection in both humans and animals. 相似文献
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Laryngeal electromyography and prognosis of unilateral vocal fold paralysis—A long‐term prospective study 下载免费PDF全文
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Martin C.S. Wong Rungsun Rerknimitr Khean Lee Goh Takahisa Matsuda Hyun-Soo Kim Deng-Chyang Wu Kai Chun Wu Khay Guan Yeoh Vui Heng Chong Furqaan Ahmed Jose D. Sollano Jayaram Menon Han-Mo Chiu Jingnan Li Jessica Y.L. Ching Joseph J.Y. Sung 《Clinical gastroenterology and hepatology》2021,19(1):119-127.e1
67.
Wuttikon Rodkvamtook Zhiwen Zhang Chien-Chung Chao Erin Huber Dharadhida Bodhidatta Jariyanart Gaywee John Grieco Narongrid Sirisopana Manerat Kityapan Michael Lewis Wei-Mei Ching 《The American journal of tropical medicine and hygiene》2015,92(5):967-971
We developed a rapid dot–enzyme-linked immunosorbent assay (dot-ELISA) using the combination of recombinant 56-kDa protein antigens that exhibited broad reactivity with serum antibodies against the four most prevalent strains (Karp, Kato, Gilliam, and TA763) of Orientia tsutsugamushi. The assay is rapid (30 minutes), and can be done at room temperature, and results can be read by the naked eye. Only a simple shaker is required to wash the membrane. Sera from 338 patients suspected of being ill with scrub typhus from rural hospitals around Thailand were tested using this dot-ELISA. Seventy-five (22.2%) patients were found to be positive. The sensitivity and specificity of dot-ELISA were determined using the indirect immunofluorescent assay (IFA) test as the gold standard, with the cutoff titer of immunoglobulin peroxidase conjugate M (IgM)/G (IgG) greater than 1:400/1:400. The dot-ELISA had a sensitivity of 98.5%, a specificity of 96.3%, a positive predictive value of 86.7%, and a negative predictive value of 99.6% for the acute-phase specimens. The results indicate that dot-ELISA rapid test using recombinant 56-kDa protein antigen was comparable with the IFA test and may be very useful for the diagnosis of scrub typhus in rural hospitals, where IFA is not available. 相似文献
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Jennifer Jeanne B. Vicera Yenn‐Jiang Lin Po‐Tseng Lee Shih‐Lin Chang Li‐Wei Lo Yu‐Feng Hu Fa‐Po Chung Chin‐Yu Lin Ting‐Yung Chang Ta‐Chuan Tuan Tze‐Fan Chao Jo‐Nan Liao Cheng‐I Wu Chih‐Min Liu Chung‐Hsing Lin Chieh‐Mao Chuang Chun‐Chao Chen Chye Gen Chin Shin‐Huei Liu Wen‐Han Cheng Le Phat Tai Sung‐Hao Huang Ching‐Yao Chou Isaiah Lugtu Ching‐Han Liu Shih‐Ann Chen 《Journal of cardiovascular electrophysiology》2020,31(6):1436-1447