Danshao Huaxian capsule in treatment of decompensated cirrhosis resulting from chronic hepatitis B

BACKGROUND: The prognosis of decompensated cirrhosis resulting from chronic hepatitis B is poor, and the benefits of treatment with interferon are ourweight serious sideeffects and the risk of fatal exacerbation of disease. Danshao Huaxian capsule rapidly reduces hepatitis B virus(HBV)-DNA in serum to undetectable levels. METHODS: A total of 35 patients with chronic hepatitis B and decompensated cirrhosis were treated with Danshao Huaxian 1.2g. po. tid daily. Before the treatment, HBVDNA in serum was positive in all patients. Ten patients had Child-Pugh class B and 25, class C hepatitis B. Seven patients underwent liver transplantation within 6 months of initial treatment. Of the 10 patients of class B, 5 died within 6 months, and the other 5 did not complete the treatment for some reasons; the 25 patients of class C were treated for at least 6 months (mean =19 months). RESULTS: In most of the 25 patients, liver function was improved slowly but markedly after 9 months of treatment, showing a decreased level of serum bilirubin from 67±13 to 30±4μmol/L (P<0.05, baseline vs.6 months), an increased level of serum albumin from 27±1 to 34±1 g/L(P<0.05) and a decreased level of Child-Pugh score from 10.3±0.4 to 7.5+0.5 (P<0.05). Three patients developed resistance to Danshao Huaxian because of a mutation in the YMDD motif, but liver function was not deteriorated. Inhibition of viral replication with Danshao Huaxian resulted in a significant improvement of liver function in patients with decompensated HBV cirrhosis, but the long-term results remain uncertain. CONCLUSION: Danshao Huaxian capsule is effective in inhibiting viral DNA replication in patients with decompensated cirrhosis and making clinical improvement.     

RUNX3、RASSF1A启动子高甲基化与胃癌进展转移的关系

目的:探讨胃癌RUNX3、RASSF1A基因启动子甲基化在胃癌进展转移中的作用及意义.方法:RT-PCR和MSP检测62例胃癌标本及56例正常胃黏膜组织RUNX3、RASSF1A基因mRNA表达及甲基化状况,免疫组织化学检测VEGF在RUNX3、RASSF1A甲基化与非甲基化胃癌组织及20例正常组织中的表达,并分析RUNX3、RASSF1A甲基化与VEGF表达的关系.结果:胃癌组织RUNX3与RASSF1A表达较正常组织均明显降低(0.629±0.461 vs 0.893±0.543,0.653±0.476 vs 0.858±0.581,均P<0.05),且RUNX3与RASSF1A甲基化率均高于正常组织(69.4%VS 26.8%,66.1%vs 23.2%.均P<0.01).胃癌组织中RUNX3、RASSF1A甲基化组mRNA表达量较非甲基化组明显降低(0.545±0.299 vs 0.736±0.291,0.562±0.208 vs 0.674±0.185,均P<0.05).RASSF1A甲基化与肿瘤TNM分期及浸润深度相走RUNX3甲基化与肿瘤淋巴结转移、血管侵犯及TNM分期相关(P<0.05).RUNX3甲基化组胃癌组织VEGF蛋白表达高于非甲基化组(86.0%vs57.9%),RUNX3甲基化与VEGF表达相关(P<0.05).结论:RUNX3、RASSF1A启动子高甲基化可能是导致其表达降低的原因,并与胃癌进展演变相关.RUNX3甲基化可能参与胃癌血管、淋巴管转移.     

Arsenic-Induced QT Dispersion is Associated with Atherosclerotic Diseases and Predicts Long-Term Cardiovascular Mortality in Subjects with Previous Exposure to Arsenic: A 17-Year Follow-Up Study

Chronic arsenic poisoning is a major worldwide public health problem. Recently, we had reported chronic arsenic poisoning was dose-dependently associated with ventricular abnormalities quantified by electrocardiographic QT prolongation linking to atherosclerotic diseases. An association of chronic arsenic poisoning with ventricular repolarization inhomogeneity quantified by QT dispersion (QTD) is of particular interest from a theoretical and practical perspective. We aimed to further elucidate (1) the association of chronic arsenic exposure with ventricular abnormalities quantified by QTD, (2) the association of QTD with atherosclerotic diseases and (3) the predictability of QTD for long-term mortality in subjects with chronic arsenic poisoning. We followed up 280 men and 355 women living in arseniasis-endemic area in southwestern coast in Taiwan for 17 years. QTD in electrocardiogram and carotid intima–media thickness by ultrasonography were measured. Coronary artery disease was diagnosed by an abnormal electrocardiogram and a definite history. Cumulative arsenic exposure was significantly associated with QTD showing a dose–response relationship (P < 0.001). Significant associations of the QTD with coronary artery disease and carotid atherosclerosis existed after adjustment for potential confounders in the multiple linear regression analysis (all P values < 0.05). In the multivariate Cox regression analyses, the hazard ratios (95% confidence interval, P value) of cumulative cardiovascular and all-cause mortality were 3.9 (2.1–6.2, P = 0.002) and 1.4 (0.9–2.3, P = 0.10), respectively, for QTD ≥ 65 ms compared with QTD < 65. QTD may be indicated as an early biomarker for atherosclerotic diseases and a significant and strong predictor of cardiovascular mortality in population with chronic arsenic exposure.     

护理干预对院外高血压患者的影响

目的探讨护理干预对院外高血压病患者的影响。方法 2008年1月至2009年12月,在滨州市结核病防治院体检、门诊以及住院人群中选择符合2009年基层版《中国高血压防治指南》制定的原发性高血压标准,SBP≥145mmHg或(和)DBP≥95mmHg患者530例作为研究对象。通过电话、走访以及电脑视频、电子邮件等方式进行护理干预,干预以1年为期进行分析,其内容是:宣教防治高血压病的知识,纠正不健康的生活方式、不规范的用药、情绪不稳定等。结果除情绪不稳定(P>0.05)外,其余经干预结果满意(P<0.01),血压下降有效率达87.55%。结论护理干预对院外高血压患者效果良好。     

基于等温滴定量热技术表征的中药注射剂临床联合用药相容性评价

为建立一种快速评价中药注射剂临床联合用药相容性的方法,采用等温滴定量热法(isothermal titration calorimetry,ITC),考察模式药益气复脉冻干粉针(YQFM)与常用联合用药维生素C注射液(Vc)及5%葡萄糖注射液(5%GS)的相容性,以热力学参数吉布斯自由能(ΔG)、焓变(ΔH)、熵变(ΔS)判断溶合反应类型,以反应活性谱判断反应热量变化,辅以化学特征色谱法进行佐证。结果显示,YQFM与Vc溶合过程中│ΔH│>T│ΔS│,为焓驱动反应,且反应活性谱显示两者溶合放出大量热,即溶合过程中化学反应起主导作用,活性成分发生质变;与5%GS溶合过程│ΔH│相似文献   

中药学硕士专业学位人才培养模式的构建

分析中药学专业学位硕士研究生培养模式的设置意义与存在问题,在借鉴国际先进药学教育培养方式的基础上,提出初步构建我国中药学专业学位研究生培养模式的主要思路与举措。     

Interleukin-2 rescues helpless effector CD8+ T cells by diminishing the susceptibility to TRAIL mediated death

CD8(+) T cells primed in the absence of CD4(+) T cell help are programmed to produce TRAIL, which results in Death receptor (DR5) mediated apoptosis upon restimulation. Here, we studied whether these 'helpless' effector CD8(+) T cells are consigned to an apoptotic fate or whether their helpless program can be altered by inflammatory or growth cytokines. We found that helpless CD8(+) T cells regained their full proliferative and functional capacity only when IL-2 was added to cell cultures, while IL-7 and IL-15, two common gamma chain cytokines associated with CD8(+) T cell homeostasis and memory, could only partly restore secondary expansion in helpless CD8(+) T cells. Recovery of functional CD8(+) T cell immunity by IL-2 was concomitant with induction of IL2Rα (CD25) expression, downregulation of TRAIL, and the upregulation of anti-apoptotic molecules Bcl-2 and FLIP. The addition of IL-2 to helpless CD8(+) T cells also interfered with DR5-mediated apoptosis induction, indicating that IL-2 affects several components of the TRAIL-DR5 pathway. Collectively, these data demonstrate that the helpless phenotype is not fixed, and that IL-2R signaling at the time of reactivation can play an important role in restoring CD8(+) T cell function.     

HSV-TK基因和IL-12基因治疗裸鼠鼻咽癌的实验研究

目的：研究腺病毒介导的KDR启动子-单纯疱疹病毒胸苷激酶（HSV—tk）系统（AdKDR—tk）联合白细胞介素12基因对裸鼠鼻咽癌的治疗作用．方法：建立鼻咽癌裸鼠模型,将36只成瘤鼠随机分成IL-12基因组、AdKDR—tk／GCV组、IL-12基因＋AdKDR—TK／GCV组和生理盐水／GCV组（对照组）,每组9只．采用瘤内注射分别给予相对应的重组腺病毒液及生理盐水,24h后重复注射1次．次日起连续10d每天ip GCV1次,100mg／kg．治疗结束后处死裸鼠,检测肿瘤质量、组织形态学变化及肿瘤微血管密度．结果：IL-12基因组裸鼠鼻咽癌瘤体质量（1．82±0．78）g及肿瘤微血管密度（15．54±3．46）个／mm^3和AdKDR—tk／GCV组[分别为（1．61±0．61）g,（10．69±4．12）＋／mm^3]与IL-12基因＋AdKDR—TK／GCV组[分别为（1．04±0．52）g,（6．53±1．61）个／mm^3]相比差异有显著性（P〈0．05）;IL-12基因组,AdKDR—tk／GCV组及IL-12基因＋AdKDR—TK／GCV组与生理盐水GCV组[分别为（2．52±1．18）g,（22．78±5．12）个／mm^3]比较也存在显著差异（P〈0．05）．结论：HSV-TK基因和IL-12基因治疗可抑制裸鼠鼻咽癌皮下移植瘤的生长,提高机体的抗肿瘤免疫应答,两者联合运用可产生协同效应,为鼻咽癌的治疗提供新的方法．