T7 RNA聚合酶催化的荧光扩增技术检测人CEA

目的 建立检测人CEA的T7 RNA聚合酶催化的荧光扩增技术.方法 以亲和素作为连接分子,连接生物素化的检测抗体和生物素化的DNA,加入T7RNA聚合酶进行转录扩增反应,对生成的RNA产物进行荧光检测,并同时进行夹心ELISA方法检测人CEA.结果 成功的建立了检测人CEA的T7 RNA聚合酶催化的荧光扩增技术,其检测CEA的灵敏度达2×10 -3 ng/ml,比夹心EL ISA 方法灵敏度高125倍.结论 T7 RNA聚合酶催化的荧光扩增技术较夹心ELISA方法具有更高的敏感性,有可能作为一种新的检测方法用于临床的早期诊断.     

Anatomic and spiral computed tomographic study of the genial tubercles for genioglossus advancement.

OBJECTIVE: To measure and compare Chinese mandibular genial tubercles measured anatomically and with computed tomography (CT). STUDY DESIGN AND SETTING: Spiral CT scans were taken of 40 adult human skulls; the superior genial spines were measured using anatomic and CT methods. RESULTS: The height and width of the superior genial spines, mandible thickness, and distance from the menton to the inferior and superior margins of the superior genial spines were 5.82 +/- 0.71, 6.98 +/- 1.35, 11.95 +/- 1.59, 11.08 +/- 2.05, and 16.91 +/- 2.30 mm from anatomic measurements and 6.17 +/- 0.71, 7.01 +/- 1.13, 12.19 +/- 1.64, 10.41 +/- 1.55, and 15.73 +/- 2.12 mm using spiral CT, respectively. The anatomic and CT measurements were correlated. CONCLUSION: Spiral CT of the genial tubercles can help locate the osteotomy in genioglossus advancement. SIGNIFICANCE: This study acquired reference data on Chinese genial tubercles demonstrating that CT measurements of the genial tubercles reflect their anatomy, which should allow accurately locate the osteotomy.     

辅助发音器治疗腭咽闭合不全前后舌位置及运动特点比较研究

目的:讨论S-A治疗前后舌位置及运动形态对腭裂患者的影响。方法:采用标准头颅侧位X线片对腭裂术后VPI患者,S-A治疗前后、语音治疗一年后舌静止及运动形态进行了研究。结果:静止位S-A治疗后舌背明显降低,语音治疗后舌位逐渐抬高,发/a:/音时S-A治疗后及语音治疗一年后,不良代偿习惯逐渐消除,语音清晰度明显升高。结论:VPI的矫正及语音治疗对根除舌不良代偿习惯,获得清晰准确的发音具有重要意义。     

重度失水所致应激性高血糖症

本文报道2例肠梗阻和1例有机磷农药中毒患者出现暂时性高血糖症。该3例患者均无糖尿病史,因有重度失水,经大量补液纠正失水后血糖迅速恢复正常。口服葡萄糖耐量试验正常。作者认为非糖尿病患者可因重度失水而致应激性高血糖症。     

醋酸棉酚对大鼠肾皮质形态结构的影响

醋酸棉酚每天30mg/kg(6次/周)给药6～8周后,大鼠肾曲小管线粒体肿胀,嵴模糊,伴有早期溶酶体增多、增大,形态多样,其后转而减少、变小。肾近曲小管微绒毛水肿,部分肾曲小管基底膜水肿。未见肾小球毛细血管内皮细胞、基膜和足细胞的超微结构明显变化。     

某些降压药对血脂的影响

报道52例高血压病人服降压药双氢克尿噻、心得安、复方降压片和硝苯啶1～2月后血脂的变化,结果显示,双克(25～50mg/日)对血脂的主要影响是升高TG,加用心得安(30mg/日)后,TG升高幅度更大;TC有上升趋势,HDL-C变化较小。复降片(3片/日)对血脂的影响较大。不仅TG明显升高、TC也有明显升高,而HDL-C则有显著下降、HDL-C/TC比值下降也十分显著。硝苯啶(30mg/日)对血脂也产生不利影响,血TG浓度上升,TC浓度增加,而HDL-C变化不大。这种变化已被看作为对长期接受这些药物治疗的病人外加了一个致动脉粥样硬化的危险因素。     

沈阳市某农村社区居民健康状况分析

目的：了解沈阳市农村社区居民健康状况及主要健康问题,为农村健康促进工作提供基础资料。方法：采用随机抽样的方法,选择东陵区某中等经济水平农村社区18岁以上成年人进行一般状况调查及健康体检。结果：本社区居民超重和肥胖、高血粘、血压异常、脂肪肝、咽炎、尿路感染、女性阴道炎检出率较高,分别为48.0%,41.5%,33.8%,27.1%,20.7%,7.0%,47.5%。结论：该社区需加强健康教育和健康促进为主导的综合干预措施,进一步促进农村社区人群健康状况。     

乳腺导管扩张症的诊断与鉴别(附6例临床病理报道)

本文对6例乳腺导管扩张症进行了临床病理报道,6例术前皆被误诊乳腺其他疾病,根据此种情况作了如何正确诊断的阐述.提出了常见误诊病例如何鉴别。     

Postoperative deep vein thrombosis in the Taiwanese Chinese population

Reports of the incidence of postoperative deep vein thrombosis in the Chinese population of Taiwan are few in the literature. Over a 3 year period, the fibrinogen degradation products test, the pulse volume recorder, and venography were used to study 220 patients undergoing major operations at the Veterans General Hospital in Taipei, Taiwan. Deep vein thrombosis was found in 17 patients (7.7 percent). A comparison of the three diagnostic methods showed that the sensitivities of the fibrinogen degradation products test and the pulse volume recorder were 56.3 percent and 93.7 percent, respectively; the specificities, 97.4 percent and 95.7 percent, respectively; and the accuracies, 85.5 percent and 95.5 percent, respectively. These results support the combined use of the fibrinogen degradation products test and the pulse volume recorder for screening and diagnosing deep vein thrombosis.