涎腺肌上皮癌的生物学行为及治疗

为了总结涎腺肌上皮癌的生物学行为及有效治疗，作者对１９例涎腺肌上皮癌患者进行临床分析，其临床特点为：发病部位以腮腺为最常见，其次为腭部；部分肿瘤生长迅速，广泛侵犯周围组织；颈部淋巴结转移率不很高，但血行性转移率高；治疗后极易复发；患者预后差。其生物学行为属高度恶性肿瘤。治疗以根治性切除为主，原则上不作选择性颈淋巴清扫术；放射治疗不敏感。对于局限性肿瘤复发灶及时手术可获得较好的效果。     

Effect of Dynamic Loading Methods on Cement Film Thickness In Vitro

Purpose Reduced cement film thicknesses can improve crown seating and decrease marginal discrepancies. Improved marginal adaptation has the potential to reduce plaque accumulation, periodontal disease, and cement dissolution. Studies have indicated that dynamic seating methods can reduce seating discrepancies associated with zinc phosphate and resin cements. However, other types of cements and other dynamic techniques have not yet been studied or compared, nor has the mechanism for improved seating been fully explained. Therefore, the purpose of this study was to investigate the effect of a variety of loading methods on the film thicknesses of current types of crown and fixed partial denture cements compressed between glass plates. Materials and Methods This study investigated the effect of three dynamic loading methods on film thickness of six representative fixed prosthodontic cements. These included zinc phosphate, resin-modified glass ionomer, encapsulated glass ionomer, adhesive composite resin, composite resin, and polycarboxylate. The method was derived from American Dental Association specifications for cement film thickness. In control groups, the cements were placed between two glass plates and statically loaded with a 15-kg weight. The test groups were initially similarly loaded, and then for 30 seconds further subjected to simulated repeated patient opening and closing, vibrations from an electromallet, or an ultrasonic device. Results Mean film thicknesses ranged from 7.4 μm for polycarboxylate/ultrasound up to 40.3 μm for composite resin/static. Two-way analysis of variance revealed that the effects of material type and cementation method and their interaction all significantly affected film thickness (P < .0001). Multiple range analysis showed that dynamic methods were generally superior to static loading and that the ultrasonic method was the best overall. Conclusions The different dynamic loading methods all significantly decreased cement film thicknesses between glass plates. The ultrasonic method was the most effective. The type of cement used also influenced film thickness. Composite resins were more affected than other materials.     

纳米根管充填材料根尖封闭能力的实验研究

目的 观察纳米材料根充后根尖微渗漏请况，评价其根尖封闭能力。方法 30颗离体单根管牙牙根，随机分为三组，每组10个，预备完成的根管用牙胶尖和一种根管糊荆、采用侧方加压法进行充填。根据染料在牙根中的渗入深度，评价根尖微渗漏情况。结果 三种材料的根尖微渗漏有显著差异（P〈0．01）；n-ZO组的根尖微渗漏最小，n-HA组次之，ZOE组最大。结论 纳米氧化锌糊剂和纳米羟磷灰石糊剂具有良好的根尖封闭性，可以作为根管封闭剞用于根管充填。     

CT辅助咽后壁瓣手术设计

目的 建立CT辅助设计咽后壁瓣的方法,以提高治疗效果。方法 应用CT测量26例腭咽闭合不全者在静止和发“i”时腭咽腔的宽度和长度,观察分析咽后壁瓣成形术所需的宽度、长度和咽后壁瓣蒂的位置和与软腭的附着位置。结果 静止时腭咽腔的宽度15.10-37.25mm,平均27.01mm,长度12.54-30.39mm,平均22.10mm;发“i”时腭咽腔的宽度为9.11-34.06mm,平均17.69mm;咽后壁瓣蒂的位置都在第一颈椎平面或以上;与软腭的附着位置大部分在软腭的中后1/3处。结论 CT作为一种定量评价腭咽闭合功能的方法,能够辅助设计咽后壁瓣手术方法。     

Er,Cr:YSGG激光照射对窝沟封闭剂粘结性的影响

目的 :探讨Er,Cr:YSGG激光照射作为窝沟封闭前釉质预处理方式的可能性。方法 :以 40颗离体前磨牙做为标本 ,随机分成 4组 ,唇面釉质分别采用下列方式处理 ,A组 :杯状刷处理 酸蚀 ,B组 :钻切割 酸蚀。C组 :Er ,Cr :YSGG激光照射 ,D组 :Er,Cr :YSGG激光照射 酸蚀。测试窝沟封闭剂与釉质面的抗剪强度 ,并用扫描电镜观察 4种方式处理后釉质表面的形态特征。结果 :Er ,Cr :YSGG激光照射与传统杯状刷或钻处理后酸蚀的釉质面抗剪强度相似无显著性差异 (P >0 .0 5 ) ,Er ,Cr:YSGG激光照射后酸蚀也不增大抗剪强度。SEM观察Er ,Cr :YSGG激光照射后的釉质面不规则 ,鱼鳞状 ,无玷污层。结论 :Er ,Cr :YSGG激光照射可代替传统杯状刷或钻处理后酸蚀 ,是一种较好的窝沟封闭前预处理方式     

变形链球菌CH43变链素Ⅰ基因片段的克隆及表达

目的:获得编码变形链球菌CH43变链素Ⅰ前体肽基因片段,并诱导其在不杀伤工程菌的前提下高效表达。方法:用PCR技术从变形链球菌CH43基因库中扩增出编码变链素Ⅰ成熟肽mutA基因片段相应大小的DNA片段,将片段与pMD18 T载体连接后测序。将测序正确的mutA按照BamHⅠ和HindⅢ酶切位点克隆入原核表达载体pProEX,将连接产物转化入E.coliDH5α,挑出阳性克隆,IPTG诱导表达重组的6×His融合蛋白。以IPTG浓度、A600值、诱导时间各梯度优化表达。结果:PCR获得的mutA序列与GenBank报道的一致(为147bp)。优化了诱导条件使重组载体在E.coliDH5α中高效表达,融合蛋白经SDS PAGE,在相对分子质量为5. 7×103处有特异的蛋白条带,在A600为1. 666时,加入IPTG至终浓度为1. 0mmol/L,诱导6h,目的蛋白表达量最高,占菌体总蛋白量的20%左右。结论:成功克隆变形链球菌CH43变链素ⅠmutA基因片段,并在E.coliDH5α中高效表达。     

Can anti‐erosion dentifrices also provide effective plaque control?

To cite this article:
Int J Dent Hygiene 9 , 2011; 223–228
DOI: 10.1111/j.1601‐5037.2010.00480.x
Bellamy PG, Prendergast M, Strand R, Yu Z, Day TN, Barker ML, Mussett AJ. Can anti‐erosion dentifrices also provide effective plaque control? Abstract: Objective: While gingivitis and caries continue to be prevalent issues, there is growing concern about dental erosion induced by dietary acids. An oral hygiene product that protects against all these conditions would be beneficial. This study investigated the potential of two anti‐erosion dentifrices to inhibit plaque. Methods: This was a randomized, three‐period, two‐treatment, double‐blind, crossover study evaluating a stannous chloride/sodium fluoride dentifrice (SnCl₂/NaF, blend‐a‐med^® Pro Expert) and a popular anti‐erosion dentifrice (NaF, Sensodyne^® ProNamel^?). During Period 3, subjects were randomized to repeat one treatment to evaluate any product carryover effects. Each treatment period was 17 days. Test dentifrices were used with a standard manual toothbrush. Digital plaque image analysis (DPIA) was employed at the end of each period to evaluate plaque levels (i) overnight (am prebrush); (ii) post‐brushing with the test product (am post‐brush); and (iii) mid‐afternoon (pm ). Analysis was conducted via an objective computer algorithm, which calculated total area of visible plaque. Results: Twenty‐seven subjects completed the study. At all time points, subjects had statistically significantly (P ≤ 0.0001) lower plaque levels after using the SnCl₂/NaF dentifrice than the NaF dentifrice. The antiplaque benefit for the SnCl₂/NaF dentifrice versus the NaF dentifrice was: am prebrush = 26.0%; am post‐brushing = 27.9%; pm = 25.7%. Conclusions: The SnCl₂/NaF dentifrice provided significantly greater daytime and overnight plaque inhibition than the NaF toothpaste. When recommending dentifrice to patients susceptible to dental erosion, clinicians can consider one that also inhibits plaque.     

口呼吸与鼻呼吸儿童牙弓、基骨形态差异的研究

目的：比较口呼吸与鼻呼吸儿童牙弓、基骨形态的差异，探讨呼吸方式与牙弓、基骨形态的关系。方法：通过口鼻气流同步测定系统的随机检测，选择鼻呼吸比例≤70％(口呼吸组)及≥95％(鼻呼吸组)的11～14岁儿童各34人，测量石膏模型，比较其牙弓、基骨形态的差异。结果：①口呼吸儿童与鼻呼吸儿童相比腭盖较高，上颌基骨较窄，形态相对窄长。②口呼吸儿童比鼻呼吸儿童的上颌中、后段牙弓较长，牙弓形态相对窄长，下颌牙弓后段形态也相对窄长。结论：口呼吸可以引起儿童牙弓、基骨形态的改变。     

Ultrastructural study of adenolymphoma

Ultrastructure of 10 adenolymphomas of parotid gland were studied. The neoplastic epithelium consists of "oncocytes" which are arranged in pseudostratified pattern. Their cytoplasm is almost totally packed with a large numbers of hypertrophic mitochondria showing a variety of peculiar forms. The amount and alteration of the mitochondria gradually increase in the order of pyramidal cell, common columnar cell and degenerated columnar cell. This might be a process of transposition representing degeneration due to metabolic disorder in the tumor. The obvious alterations of mitochondria are found in the cases with not only "hot" nodule but also "cold" nodule shown in the studies of 99mTc scintigraphy. There seems no relationship between "hot" nodule and alteration of mitochondria.