全文获取类型
收费全文 | 4447篇 |
免费 | 253篇 |
国内免费 | 43篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 85篇 |
妇产科学 | 57篇 |
基础医学 | 623篇 |
口腔科学 | 99篇 |
临床医学 | 255篇 |
内科学 | 1168篇 |
皮肤病学 | 397篇 |
神经病学 | 207篇 |
特种医学 | 145篇 |
外科学 | 934篇 |
综合类 | 16篇 |
预防医学 | 101篇 |
眼科学 | 36篇 |
药学 | 184篇 |
中国医学 | 11篇 |
肿瘤学 | 414篇 |
出版年
2023年 | 25篇 |
2022年 | 73篇 |
2021年 | 133篇 |
2020年 | 73篇 |
2019年 | 89篇 |
2018年 | 135篇 |
2017年 | 95篇 |
2016年 | 94篇 |
2015年 | 127篇 |
2014年 | 158篇 |
2013年 | 177篇 |
2012年 | 308篇 |
2011年 | 315篇 |
2010年 | 177篇 |
2009年 | 157篇 |
2008年 | 258篇 |
2007年 | 271篇 |
2006年 | 274篇 |
2005年 | 262篇 |
2004年 | 258篇 |
2003年 | 291篇 |
2002年 | 278篇 |
2001年 | 44篇 |
2000年 | 48篇 |
1999年 | 54篇 |
1998年 | 68篇 |
1997年 | 56篇 |
1996年 | 49篇 |
1995年 | 39篇 |
1994年 | 42篇 |
1993年 | 32篇 |
1992年 | 29篇 |
1991年 | 35篇 |
1990年 | 12篇 |
1989年 | 17篇 |
1988年 | 26篇 |
1987年 | 14篇 |
1986年 | 16篇 |
1985年 | 19篇 |
1984年 | 12篇 |
1983年 | 11篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 8篇 |
1979年 | 10篇 |
1978年 | 8篇 |
1976年 | 5篇 |
1974年 | 10篇 |
1972年 | 6篇 |
1971年 | 5篇 |
排序方式: 共有4743条查询结果,搜索用时 15 毫秒
71.
Hiroya Takeuchi Ken Futamata Arafumi Maeshima Shigemichi Hirose Atsushi Shimada Yoshiki Kawaguchi Jo Tokuyama Koji Osumi Shinya Kishi Takashi Hojo Shiei Kim Norihito Wada Takashi Oishi Yoh Isobe Shunji Ikeuchi Kiyoshi Kubochi Sumio Matsumoto 《Journal of gastrointestinal cancer》2005,36(2):99-104
We report a very rare case of primary gastric small cell carcinoma (GSCC) that was accompanied with gastric tubular adenocarcinoma. A male in his 60s had an elevated tumor with a central ulceration in the middle stomach. The patient underwent a distal gastrectomy with lymph node dissection. The pathological examination showed two separated lesions of the stomach, which contained the components of primary GSCC and primary gastric tubular adenocarcinoma. Immunohistochemical (IHC) examination demonstrated that the tumor cells in the small cell carcinoma stained positive for synaptophysin, chromogranin A, and neural cell adhesion molecule (NCAM). GSCC cells and adenocarcinoma cells independently metastasized to each regional lymph node. Further studies on the biological behavior of individual tumors may allow the development of new treatment strategies for GSCC. 相似文献
72.
Recently, highly sensitive prostate specific antigen (PSA) kits have been developed and reported to be useful for the early identification of a chemical relapse. However, if the measurement time was short and the cost low, such an assay kit should be sufficient for cancer screening when dealing with a large number of samples. The One Step PSA test uses an immunochromatographic method to qualitatively, not quantitatively, judge a positive or negative result. We confirmed the sensitivity of the kit using purified PSA. Serum specimens from 147 men with or without prostate diseases were tested using the kit. PSA concentration of each serum specimen was independently measured by a quantitative ACS-PSA2 EIA kit (Chiron, cut-off: 2.1 ng/ml). The sensitivity of this kit was determined to be 4 ng/ml. All 33 samples with a value of greater than 4 ng/ml were clearly positive. Of the 94 samples with values less than 4 ng/ml, nine were judged as positive. The remaining 85 cases were judged as completely negative. These results indicate that the sensitivity of the One Step PSA test is 100% and the specificity is 90.4%. Tests using this kit can be easily performed at outpatient clinics or elsewhere. This kit is useful for initial cancer screening, because results can be obtained within 15 min and at a cost lower than that of ordinary PSA kits. 相似文献
73.
Terazaki Y Yoshiyama K Matsueda S Watanabe N Kawahara A Naito Y Suekane S Komatsu N Ioji T Yamada A Mine T Terasaki M Itoh K Takamori S Sasada T 《Cancer science》2012,103(4):638-644
Since the prognosis of small cell lung cancer (SCLC) remains poor, development of new therapeutic approaches, including immunotherapies, would be desirable. In the current study, to evaluate immunological responses in refractory SCLC patients, we conducted a small scale phase II clinical trial of personalized peptide vaccination (PPV), in which vaccine antigens are selected based on pre-existing host immunity. Ten refractory SCLC patients, who had failed to respond to chemo- and/or chemoradiotherapies (median number of regimens, 2.5; median duration, 20.5 months), were enrolled. A maximum of four human leukocyte antigen (HLA)-matched peptides showing higher antigen-specific humoral responses were subcutaneously administered (weekly for six consecutive weeks and then bi-weekly thereafter). PPV was terminated before the 3rd administration in four patients because of rapid disease progression, whereas the remaining six patients completed at least one cycle (six times) of vaccinations. Peptide-specific immunological boosting was observed in all of the six patients at the end of the first cycle of vaccinations, with their survival time of 25, 24.5 (alive), 10 (alive), 9.5, 6.5, and 6 months. Number of previous chemotherapy regimens and frequency of CD3(+) CD26(+) cells in peripheral blood were potentially prognostic in the vaccinated patients (hazard ratio [HR] = 2.540, 95% confidence interval [CI] = 1.188-5.431, P = 0.016; HR = 0.941, 95% CI = 0.878-1.008, P = 0.084; respectively). Based on the feasible immune responses in refractory SCLC patients who received at least one cycle (six times) of vaccinations, PPV could be recommended for a next stage of larger-scale, prospective clinical trials. 相似文献
74.
Yasuto Sasaki Yoshiki Shiba Chikara Hirono Yoshinobu Kanno Tomio Takeuchi Kazuo Umezawa 《Cancer science》1989,80(9):855-860
We have examined the suppressive effect of herbimycin A on the reduction of gap-junctional Intercellular communication that is induced by a tumor-promoting phorbol ester in 3T3-L1 cells. Most cells in growth arrest participated in dye-coupling, as evaluated by the transfer between cells of a fluorescent dye (Lucifer Yellow CH). Treatment of cells with 0.25 μg/ml herbimycin A slightly enhanced the dye-coupling. This enhancement required treatment for periods as long as 24 h. Addition of 100 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a rapid reduction of dye-coupling. However, addition of TPA did not suppress dye-coupling in cells pretreated for more than 24 h with herbimycin A. Pretreatment of cells for less than 6 h with herbimycin A did not suppress the TPA-induced reduction of dye-coupling. These results suggest that herbimycin A suppresses the reduction of gap-junctional intercellular communication that is induced by TPA through enhancement of the ability of the cells to participate in gap-junctional intercellular communication 相似文献
75.
Masaki Nakamura Shuji Kameyama Yoshiki Ambe Taro Teshima Taro Izumi Ibuki Tsuru Yasushi Inoue Tadashi Yoshimatsu Hiroki Inatsu Ryo Amakawa Masashi Kusakabe Teppei Morikawa Yoshiyuki Shiga 《Translational andrology and urology》2022,11(9):1226
BackgroundThere is limited information on perioperative renal function during off-clamp, non-renorrhaphy open partial nephrectomy. Therefore, this retrospective study aimed to identify predictive factors of perioperative decline in renal function after off-clamp, non-renorrhaphy open partial nephrectomy.MethodsClinical records of 138 patients with renal tumors who underwent off-clamp, non-renorrhaphy open partial nephrectomy at our institution were reviewed. Off-clamp, non-renorrhaphy partial nephrectomy was performed using a soft coagulation system. Perioperative estimated glomerular filtration rate (eGFR) preservation was calculated, and predictors were identified using multivariate regression analysis at 5 days, 1 month, and 3 months after surgery.ResultsThe median operation time was 122 minutes, and the median volume of estimated blood loss was 155 mL. The mean eGFR preservation at 5 days, 1 month, and 3 months after surgery was 95.3%, 91.0%, and 90.7%, respectively. Estimated blood loss was an independent predictor of perioperative decline in eGFR 5 days after surgery [odds ratio (OR): 0.97; 95% confidence interval (CI): 0.96, 0.98; P<0.001]. Preoperative eGFR and estimated blood loss were independent predictors of perioperative decline in eGFR 1 month after surgery (OR: 0.86; 95% CI: 0.77, 0.95; P=0.007 and OR: 0.98; 95% CI: 0.97, 0.99; P<0.001, respectively). Age, preoperative eGFR, and estimated blood loss were independent predictors of perioperative decline in eGFR 3 months after surgery (OR: 0.64; 95% CI: 0.54, 0.81; P<0.001, OR: 0.72; 95% CI: 0.61, 0.85; P<0.001; and OR: 0.98; 95% CI: 0.97, 0.99; P=0.004, respectively).ConclusionsEstimated blood loss during surgery was a predictor of perioperative decline in eGFR within 3 months after off-clamp, non-renorrhaphy open partial nephrectomy. Age was a predictor of perioperative decline in eGFR 3 months after surgery. 相似文献
76.
Taku Miyanaga Kohei Tokuyama Chiharu Mizoguchi Tsutomu Daa Yoshihiro Kusaba Yoshiki Asayama 《Radiology Case Reports》2022,17(11):4209
Neuroendocrine carcinoma of the uterine endometrium is extremely rare and found in <1% of all primary endometrial carcinomas. We report a case of neuroendocrine carcinoma of the endometrium detected in a 65-year-old woman and focus our attention on the main imaging features. The low apparent diffusion coefficient value and high maximum standardized uptake value for neuroendocrine cancer serve to distinguish this cancer from endometrial cancer. 相似文献
77.
Hiroyuki Hisada Yoshiki Sakaguchi Kaori Oshio Satoru Mizutani Hideki Nakagawa Junichi Sato Dai Kubota Miho Obata Rina Cho Sayaka Nagao Yuko Miura Hiroya Mizutani Daisuke Ohki Seiichi Yakabi Yu Takahashi Naomi Kakushima Yosuke Tsuji Nobutake Yamamichi Mitsuhiro Fujishiro 《Current oncology (Toronto, Ont.)》2022,29(7):4678
Although the mortality rates of gastric cancer (GC) are gradually declining, gastric cancer is still the fourth leading cause of cancer-related death worldwide. This may be due to the high rate of patients who are diagnosed with GC at advanced stages. However, in countries such as Japan with endoscopic screening systems, more than half of GCs are discovered at an early stage, enabling endoscopic resection (ER). Especially after the introduction of endoscopic submucosal dissection (ESD) in Japan around 2000, a high en bloc resection rate allowing pathological assessment of margin and depth has become possible. While ER is a diagnostic method of treatment and may not always be curative, it is widely accepted as standard treatment because it is less invasive than surgery and can provide an accurate diagnosis for deciding whether additional surgery is necessary. The curability of ER is currently assessed by the completeness of primary tumor removal and the possibility of lymph node metastasis. This review introduces methods, indications, and curability criteria for ER of EGC. Despite recent advances, several problems remain unsolved. This review will also outline the latest evidence concerning future issues. 相似文献
78.
79.