努力拓展学生党建工作的新途径

党的十六大报告指出":必须以改革的精神推进党的建设,不断为党的肌体注入新的活力。"这一极富新意的论断,是新时期加强和改进党的建设必须牢牢把握的一个基本原则。在新形势下,应大力加强大学生党建工作,把大学生党员的思想、行动统一到十六大精神上来,并化为自觉的行动,形成同心同德的巨大向心力。坚持党要管党、从严治党的方针,切实加强学生党建工作,不断壮大学生党员队伍,加强学生党员的教育管理,充分发挥学生党支部的战斗堡垒作用和学生党员的先锋模范作用,培养具有坚定共产主义信念的青年马克思主义者,为党的事业输送新鲜血液。     

两点法腰麻-硬膜外联合阻滞用于妇科腹腔镜手术的体会

目的观察两点法腰麻-硬膜外联合阻滞用于妇科腹腔镜手术的可行性。方法观察68例,年龄34岁~75岁,ASAⅠ~Ⅱ级。全部采用两点法穿刺,先于T10~11间隙行硬外置管,后于L2~3间隙用25G腰麻针行蛛网膜下腔穿刺,成功后2ml0.75%布比卡以0.1ml/s的速度推完。腰麻平面固定后若未能达T4者硬膜外腔追加2%利多卡因。记录麻醉起效时间(注药至T4针刺无痛时间),麻醉平面上界,二氧化碳分压升高幅度,局麻药总量及麻醉并发症,并评定麻醉效果。结果麻醉效果满意,麻醉起效时间(8.4±2.9)分钟,麻醉平面上界T3~T5,局麻药总量(8±3)ml,二氧化碳分压均小于47mmHg。无麻醉并发症发生。结论本法对于妇科腹腔镜手术,起效快,效果确切,合并症少,有临床应用价值。     

低弹应力对钛合金植入物表面新骨形成的影响

目的观察应力作用下低弹β钛合金植入物表面新骨形成过程并探讨其对生物稳定的影响。方法选用20只成年新西兰大白兔,于双侧胫骨近端植入2种弹性模量(110GPa和30GPa)钛合金植入物,其中左侧为低弹组,右侧为高弹组,分别于术后4、8周处死动物,取材标本进行Micro-CT检测,测定BMD,BVF,SMI等指标,采用组织学方法,测定植入物-骨接触率(C)、单位面积新生骨百分比(S)以及生物力学最大拔出力。结果钛合金植入物植入后4周,组织学显示低弹组植入物表面新骨形成量多于高弹组,但2组之间差异无统计学意义;Micro-CT分析显示,2组之间BMD、BVF、SMI差异无统计学意义;生物力学最大拔出力检测低弹组为(74.60±6.59)N,高弹组为(63.30±8.41)N,2组之间差异存在统计学意义(P<0.05)。8周时组织学观察发现,低弹组植入物表面新骨形成量明显多于高弹组,植入物-骨接触率和单位面积新生骨百分比低弹组均优于高弹组;Micro-CTBMD、BVF、SMI分析显示低弹组材料表面成骨明显优于高弹组;低弹组的拔出力明显高于高弹组(P<0.01)。结论金属植入物的低弹特性有利于应力作用下植入物-骨界面的新骨形成,从而有利于提高植入物的生物稳定性。     

A cell-based high-throughput screening assay for Farnesoid X receptor agonists

Objective To develop a high-throughput screening assay for Farnesoid X receptor （FXR） agonists based on mammalian one-hybrid system （a chimera receptor gene system） for the purpose of identifying new lead compounds for dyslipidaemia drug from the chemical library. Methods cDNA encoding the human FXR ligand binding domain （LBD） was amplified by RT-PCR from a human liver total mRNA and fused to the DNA binding domain （DBD） of yeast GAL4 of pBIND to construct a GAL4-FXR （LBD） chimera expression plasmid. Five copies of the GAL4 DNA binding site were synthesized and inserted into upstream of the SV40 promoter of pGL3-promoter vector to construct a reporter plasmid pG5-SV40 Luc. The assay was developed by transient co-transfection with pG5-SV40 Luc reporter plasmid and pBIND-FXR-LBD （189-472） chimera expression plasmid. Results After optimization, CDCA, a FXR natural agonist, could induce expression of the luciferase gene in a dose-dependent manner, and had a signal/noise ratio of 10 and Z＇ factor value of 0.65, Conclusion A stable and sensitive cell-based high-throughput screening model can be used in high-throughput screening for FXR agonists from the synthetic and natural compound library.     

多发性创伤168例的急救与护理体会

目的探讨如何提高多发性创伤的抢救和护理水平。方法回顾性分析近3年来我院急诊的168例多发性创伤病人的临床资料。结果多发性创伤以青壮年男性居多,病情复杂凶险,病死率高达31.5%,术前、术后病死率分别为47.2%、43.4%,术前主要死于创伤性休克,术后主要死于重要脏器功能衰竭。结论急诊护士要提高对多发性创伤的认识,按照VIPCO急救程序救治,加强生命体征的监测和护理,提高救治成功率。     

首发精神分裂症患者工作记忆、执行功能的对照研究

目的 探讨首发精神分裂症对工作记忆和执行功能的影响.方法 对30例首发精神分裂症患者和21例正常对照组进行数字广度(顺背、倒背)和威斯康星卡片分类测验(WCST).结果 首发精神分裂症组在数字广度(顺背、倒背)测验成绩差于正常对照组,WCST的持续错误数大于对照组而分类个数少于对照组,两组比较有显著性差异(p＜0.05),首发精神分裂症组在给予提示后WCST结果与未提示相比有显著性差异(p＜0.05).结论 首发精神分裂症患者工作记忆尤其是瞬时记忆、短时记忆和执行功能存在一定程度损害,在康复过程应注意采取综合措施促进患者的全面康复.     

Effect of salvia miltiorrhiza Bge on left ventricular hypertrophy and the expression of tumor necrosis factor-α in spontaneously hypertensive rats

Summary The effects of salvia miltiorrhiza Bge (SMB) on left ventricular hypertrophy (LVH) and the expression of tumor necrosis factor-α (TNF-α) in the left ventricle of spontaneously hypertensive rats and the action mechanism were investigated. Normal Wistar-kyoto (WKY) rats were used as negative control, and spontaneously hypertensive rats (SHR) were randomly assigned to receive placebo or SMB. SMB (1 g/kg·d) was injected intraperitoneally for 12 weeks. Systolic blood pressure (SBP) and left ventricular mass index (LVMI) were measured. HE, VG and immunohistochemical staining combined with computed morphometry were employed to evaluate the cardiomyocyte size, diameter, the collagen volume fraction (CVF), perivascular circumferential area (PVCA), and tumor necrosis factor-α (TNF-α) expression in the left ventricular tissue. The results showed, as compared with WKY rats, the SBP, LVMI, cardiomyocyte size, diameter, CVF, PCVA, and TNF-α expression were increased markedly in the 20-week-old spontaneously hypertensive rats. SMB decreased LVMI (P<0.01), size of cardiomyocytes (P<0.01), collagen volume fraction (P<0.01), perivascular circumferential area (P<0.01), and TNF-α expression (P<0.01), but had no effect on SBP (P>0.05). It was suggested that chronic administration of SMB could inhibit and reverse the development of LVH in spontaneously hypertensive rats independent of BP. TNF-α may be involved in the reversal mechanism of LVH by SMB. SUN Lianping, male, born in 1966, Associate Professor     

吻合神经的拇指尺背侧皮神经筋膜蒂岛状皮瓣修复示指末节软组织缺损

目的观察应用吻合神经的拇指尺背侧皮神经筋膜蒂岛状皮瓣修复示指末节桡侧软组织缺损的临床效果。方法对15例示指末节桡侧软组织缺损病例,采用拇指尺背侧皮神经筋膜蒂岛状皮瓣,逆行移位至拇指近节近端尺侧,屈曲示指末节贴紧拇指近节尺侧固定,吻合拇指尺背侧皮神经与示指桡侧指固有神经,皮瓣带蒂修复创面,2、3周后断蒂。结果皮瓣全部成活,随访6～24个月,皮瓣质地柔软、弹性好、耐磨;指端饱满,色泽正常,两点辨别觉6.4￣9.6mm,11例感觉恢复至S3,4例至S4,外观及功能满意。结论应用吻合神经的拇指尺背侧皮神经筋膜蒂岛状皮瓣,术后效果良好,是修复示指末节桡侧软组织缺损较理想的手术方法。     

活用多元教学方法,提高临床医学青年教师执教能力

提出了灵活运用多元教学方法,提高临床医学青年教师执教能力的观点.并详细介绍了参与构建“学习环”,实现教学相长、学习名师授课技巧,培养独具魅力的讲课风格、践行PBL教学法,以学生为中心以及利用数字化可视化教学平台,实现直观教学等各种方法的应用.     

浅谈《黄帝内经》的人体观

通过对《内经》中"天人合一"、"形神一体"的整体人体观的分析,探讨了《内经》所体现的医学人文精神。医学人文精神推崇的是由人的多重属性和多重关系而展现出来的"人的世界",而《内经》中的身体,是和自然环境、社会环境融为一体的存在,体现了以生态的自然人为本,重视病患精神状态的人文精神。