海南粗榧新碱衍生物 HH07A 对 DNA 聚合酶 I 活性的影响

用体外DNA聚合酶I作用下DNA合成的方法,研究HH07A的作用机制。结果表明,HH07A对DNA聚合酶I催化下的DNA合成有明显抑制作用,且在一定浓度范围内存在浓度依赖性。将药物与DNA模板及DNA聚合酶I分别预保温后,发现DNA模板活性无明显改变,而酶的活性则受到显著抑制。提示HH07A对DNA聚合酶I催化下DNA合成的抑制是通过HH07A与DNA聚合酶I直接作用而实现的。     

可逆性胆碱酯酶抑制剂二甲氨基甲酸-[3-(烷氨基)烷氧基-4(5)-叔丁基]苯酯的合成

在催醒安的邻位和对位异构体及其同系物的苯坏上引入烷基后,可以增强抑制胆碱酯酶的活性,我们乃进一步在催醒安及其同系物的苯环不同位置上引入叔丁基,合成了一系列二甲氨基甲酸-[3-(烷氨基)烷氧基-4(5)-叔丁基]苯酯(Ⅰ_(1～13)和Ⅱ_(1～5))(表2),以探索对活性的影响。     

Efficacy of the Community Reinvestment Act in promoting lead abatement

The efficacy of the Community Reinvestment Act (CRA) in assisting homeowners or landlords in urban areas of New Jersey to finance lead abatement was evaluated in the study reported here, as was the effectiveness of the abatement. The study involved working with homeowners, banks, local health departments, and communities to facilitate financing for lead abatement, and collecting environmental-dust specimens from some of the participants' homes before and after abatement. Of the 113 interested subjects, 59 (52 percent) completed abatement of their homes. Of the 58 who applied for CRA loans, 21 received approvals and had the work completed. Thirty-nine of the homeowners found alternate means of financing abatement. The environmental data showed a significant decrease on the windowsills in the 4aated homes, but not on the floors. In conclusion, one-on-one counseling of homeowners about funding was associated with more than 50 percent success in completing abatement, The CRA loans' accounted for about one-third of the abated units. The lead abatement was effective in removing lead paint from the windows, but not in reducing dust lead levels on the floors.     

A simple and rapid approach for screening of SARS-coronavirus genotypes: an evaluation study

Background  

The Severe Acute Respiratory Syndrome (SARS) was a newly emerged infectious disease which caused a global epidemic in 2002–2003. Sequence analysis of SARS-coronavirus isolates revealed that specific genotypes predominated at different periods of the epidemic. This information can be used as a footprint for tracing the epidemiology of infections and monitor viral evolution. However, direct sequencing analysis of a large number of clinical samples is cumbersome and time consuming. We present here a simple and rapid assay for the screening of SARS-coronavirus genotypes based on the use of fluorogenic oligonucleotide probes for allelic discrimination.     

Oral misoprostol for cervical priming in non-pregnant women

Endometrial biopsy and hysteroscopy are important investigations in women presenting with abnormal vaginal bleeding. Endometrial biopsy is often performed as an outpatient procedure by endometrial aspiration. Difficulty in entering the internal cervical os may be encountered, especially in nulliparous women. The same problem may occur during hysteroscopy or dilatation and curettage. It is well known that use of a cervical priming agent is effective in reducing complications during cervical dilatation in pregnant women. However, its use in non-pregnant women is not well established. We compared oral misoprostol versus placebo for a cervical priming effect in non-pregnant women prior to hysteroscopy. The cumulative force required for cervical dilatation was significantly lower whereas the baseline cervical dilatation was significantly greater in the misoprostol group. We conclude that oral misoprostol is effective for pre-operative cervical dilatation in non- pregnant women.      

Mitochondrial DNA deletion in human oocytes and embryos

Mitochondrial DNA (mtDNA) deletions are present in both human oocytes and embryos. It has been found that these tissues contain a mtDNA mutation which is present in high amounts in patients with Kearns-Sayre syndrome (KSS) and progressive external ophthalmoplegia. In the present study, the frequency of this KSS deletion was investigated in human oocytes and embryos. Using a nested primer polymerase chain chian reaction (PCR) strategy, the frequency of the KSS deletion in 74 human oocytes and 137 embryos was found to be 32.8 and 8.0% respectively. Using a 'long PCR-short PCR' nested primer strategy, the frequency of the KSS deletion in 181 human oocytes and 104 embryos was found to be 47.0 and 20.2% respectively. There was no statistical correlation between the age of the patients at the time of oocyte retrieval and the presence of the deleted molecules. There was a statistical difference between the presence of the deleted molecules in oocytes versus embryos using either technique (P < 0.0001). The relevance of these findings to the accumulation of low levels of deleted mtDNA in both oocytes and embryos is discussed in this study.      

肺心病患者血浆内皮素-1和降钙素基因相关肽的水平

1　对象和方法1.1　对象　选择住院肺心病急性期患者38(男3,女12)例,年龄(65±2)岁.肺心病缓解期28(男17,女11)例,年龄(62±4)岁.所有患者病程6～21a,平均(18±6)a.均因受凉感冒、劳累等引起慢性支气管炎、肺心病急性发作、呼吸道症状加重.入院时均有明显气喘、咳痰、呼吸困难,严重者不能平卧,口唇紫绀、伴有不同程度呼吸衰竭及II°～III°右心衰竭.有5例并发上消化道出血,14例并电解质紊乱及心律失常.根据病史、症状、血气分析、胸部X线片及心电图等确定诊断,符合全国第3次肺心病专业会议诊断标准(1997,上海市).正常对照组31例,门诊健康…