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51.
本文以腺苷酸为测定物,探讨了以国产自动平衡记录仪为高效液相色谱记录并定量分析结果的方法。结果表明,从自动平衡记录上得到峰形、计算结果均与原机所带积分仪的一致。其稳定性、灵敏度和线性响应均能达到高效液相色谱检测器的要求,是一种可以代替积分仪分析结果的经济、可靠的方法。 相似文献
52.
Wang Tao Liang Zhihou Sun Shenggang Cao Xuebing Peng Hai Cao Fei Liu Hongjin Tong E-tang 《华中科技大学学报(医学英德文版)》2003,23(2):145-147
Summary To investigate the distribution of possible novel mutations from parkin gene in variant subset of patients with Parkinson’s
disease (PD) in China and explore whether parkin gene plays an important role in the pathogenesis of PD, 70 patients were
divided into early-onset group and late-onset group; 70 healthy subjects were included as controls. Genomic DNA from 70 normal
controls and from those of PD patients were extracted from peripheral blood leukocytes by using standard procedures. Mutations
of parkin gene (exon 1–12) in all the subjects were screened by PCR-single strand conformation polymorphism (SSCP), and further
sequencing was performed in the samples with abnormal SSCP results, in order to confirm the mutation and its location. A new
missense mutation Gly284Arg in a patient and 3 abnormal bands in SSCP electrophoresis from samples of another 3 patients were
found. All the DNA variants were sourced from the samples of the patients with early-onset PD. It was concluded that Parkin
point mutation also partially contributes to the development of early-onset Parkinson’s disease in Chinese.
WANG Tao, male, born in 1961, Associate Professor
This work was supported by grants from the key program of the special scientific project of Scientific & Technologic Agency
of Hubei Province (Serial No. 2001AA308B01) and the Hygienic Research Project of Hygienic Agency of Hubei province (Serial
No. WJ 01529). 相似文献
53.
检测198例各型乙肝患者血小板功能的五个项目:血小板总数、粘附试验、聚集试验、血块退缩、血小板第3因子有效性,发现各期乙肝患者血小板功能的异常有显著性差异(P<0.01).并提示乙肝患者除有血小板数量的改变外,还有质量的改变,因此,全面的血小板功能检测可作为估计乙肝患者肝损害程度的辅助指标. 相似文献
54.
Total fluorescence from arterial tissue is influenced by three factors: the absorption coefficient of tissue at a specific
excitation wavelength, the laser excitation power and the fluorescence coefficient which is related to chemical species in
tissue. These various influences were demonstrated by the following experimental results in vitro: (1) the effect of increasing
power on fluorescence intensity, (2) the total fluorescence intensity in normal aorta and plaque and (3) the effect of a chromophore
such as β-carotene on total fluorescence intensity. The fluorescence intensity of normal artery is an incremental function
of laser excitation power, and the fluorescence emission from normal artery compared to fluorescence emission from plaque
is significantly different at the same excitation power. The total fluorescence of normal artery was measured to be twice
as great as that of atheromatous plaque (relative mean ratio of 2.58±0.46 compared to unity,p<0.0002 at 488 nm; relative mean ratio of 2.57±0.51 compared to unity,p<0.0009 at 514 nm). The total fluorescence emission decreases with the increase of β-carotene content in arterial tissue (R=0.97). These emission differences, when intensified by an exogenous chromophore of β-carotene, may provide an improved guidance
signal for diagnosis of plaque from normal artery during laser angioplasty procedures. 相似文献
55.
TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz… 相似文献
56.
痛力克对癌症疼痛镇痛效果的临床观察 总被引:1,自引:0,他引:1
应用印度LUPIN公司提供的痛力克(酮酷酸氨丁三醇 )对中重度癌症疼痛30例进行镇痛效果的临床观察,有效率93%,平均显效时间9min,均数缓解时间5.1h,并用哌替啶做了同期交叉自身镇痛对比研究,结果表明:两药的镇痛效果相似(P>0.05),但痛力克的不良反应发生率明显低于哌替啶。 相似文献
57.
用乙二醇法诱导大鼠产生草酸钙结石,用45~Ca示踪测定法了解中草药复方制剂(PESM)对草酸钙结石形成的抑制作用。结果显示,实验组肾脏放射性强度明显减少(实验组3.47±3.52cpm/mg,对照组10.89±14.24cpm/mg)。干肾重量明显下降(实验组306.0±47.0mg,对照组343.1±59.4mg),说明PESM能明显抑制肾脏草酸钙结石的形成。 相似文献
58.
A study on various symptoms of chronic hepatitis treated with radix astragali and its regulative effect to levels of serum hormone 下载免费PDF全文
Ke-zhou Liu Zhong-hang Tong Ming-tai Zhang Wei-zheng Gu Zhi-rong Huang Bi-he Shen Pei-ren Cai Gui-lang Zhu Wen-nan He Lan-sheng Feng Lie-ming Ding Zhi Chen Jian-hua Jin Xiu-zhi Zhang Qian-yan Yu Shui-jin Tong Ping Ye 《中国结合医学杂志》1995,1(2):118-121
Sixty-seven cases of chronic hepatitis were treated with Radix Astragali. After treatment (2-month course), the clinical improvement rate in 38 cases of the Stagnation of the Liver-Qi and Deficiency of the Spleen type was 92.1%, and in 26 cases of the Deficiency of Liver Yin and Kidney Yin type was 88.5%, more effective than in the control group (P<0.05). The regulative effect to the levels of serum hormone was observed in the patients of the Stagnation of the Liver-Qi and Deficiency of the Spleen type treated with this medicine. The results showed that the levels of serum triiodothyronine, estradiol (female) and testosterone (male) were increased after treatment (1.40±1.38 ng/dl, 129.30±1.23 pg/ml and 496.24±1.47 ng/dl). Pre-treatment levels were 1.22±1.49 ng/dl, 104.60±1.45 pg/ml and 398. 17±1.55 ng/dl respectively (P<0.05); however, the level of serum prolactin (2.75±4.46 ng/ml) was lower after treatment than before treatment (3.20±3.82 ng/ml,P<0.05). No obvious changes were observed in the levels of serum follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, thyroxine, triiodothyronine uptake ratio and cortisol after treatment. 相似文献
59.
Influence of mild hypothermia on vascular endothelial growth factor and infarct volume in brain tissues after cerebral ischemia in rats 总被引:1,自引:0,他引:1
BACKGROUND: It has been demonstrated that mild hypothermia has obvious protective effect on both whole and local cerebral ischemia. However, the definite mechanism is still unclear for the brain protection of mild hypothermia on cerebral edema, inhibiting inflammatory reaction, stabilizing blood brain barrier, etc.
OBJECTIVE: To investigate the effect of mild hypothermia on the expression of vascular endothelial growth factor and the infarct volume after cerebral ischemia in rats, and analyze the brain protective mechanism of mild hypothermia.
DESIGN: A randomized grouping and controlled animal trial.
SETTING: Department of Neurology, People's Hospital of Yunyang Medical College.
MATERIALS: Twenty adult male SD rats of clean degree, weighing (250±30) g, were provided by the animal experimental center, School of Medicine, Wuhan University. The kits for SP immunohistochemistry were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd.
METHODS: The experiments were carried out in the laboratory of Department of Neurology, Renmen Hospital of Wuhan University from May to July 2005. ① The 20 rats were divided randomly into normal temperature group (n =10) and mild hypothermia group (n =10). Models of permanent middle cerebral artery occlusion were established with modified nylon suture embolization. The rats were assessed with the Longa standards: 0 point for without nerve dysfunction; 1 for mild neurological deficit (fore claws could no extend completely); 2 for moderate neurological deficit (circling towards the affected side); 3 for severe neurological deficit (tilting towards the affected side); 4 for coma and unconscious; 1-3 points represented that models were successfully established. The rats of the normal temperature group were fed at room temperature, and those in the mild hypothermia group were induced by hypothermia from 2 hours postoperatively, and the rectal temperature was kept at 34-35 ℃ for 72 hours. ② Measurement of infarct volume: All the rats were anesthetized by intraperitoneal injection overdose sodium pentobarbital 7 days postoperatively, and then the heads were cut down to harvest brain. The brain tissues were placed into -20 ℃ refrigerator for 20 minutes, coronal sections of 2 mm were prepared. The infarct sites were not stained, whereas normal brain tissues were stained as red. The infarct volumes were calculated by using MPLAS-500 multimedia color pathological image&&word analytical system. ③ Counting positive cells of vascular endothelial growth factor protein: The brains were harvested by cutting heads, then coronal sections of 2 mm were prepared. Routine dehydration, hyalinization, wax immersion and embedding were performed, then the detected with SP immunohistochemistry, the kits were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. The cells whose cytoplasm was yellow-brown were positive ones, a single sample as a unit, peri-ischemic site and ischemic core were selected, and the corresponding sites in controlateral hemisphere were taken as controls. Five visual fields were selected from each site to be observed under microscope, the cells were counted, and the average number of positive cells was calculated in each group. The numbers of positive cells were determined with the image analytical apparatus.
MAIN OUTCOME MEASURES: Number of the positive cells of vascular endothelial growth factor protein; Infarct volume of rat brain tissue.
RESULTS: All the 20 rats were involved in the analysis of results. ① Number of positive cells of vascular endothelial growth factor protein in brain tissue: It was obviously lower in the mild hypothermia group than in the normal temperature group [(24.02±5.05), (36.07±2.69) cells/high power visual field, P < 0.01]. ② Comparison of infarct volume of brain tissue: After MCAO, it was obviously smaller in the mild hypothermia group than in the normal temperature group [(153.25±23.14), (253.45±36.21) mm3, P < 0.01].
CONCLUSION: Mild hypothermia can inhibit the expression of vascular endothelial growth factor and decrease the volume of cerebral infarction. The inhibition of mild hypothermia on the expression of vascular endothelial growth factor may be one of the brain protective mechanisms. 相似文献
60.
To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile. 相似文献