消栓灵对下肢深静脉血栓形成患者血浆6-酮-前列腺素F1α及血栓素B2含量的影响

目的观察消栓灵对下肢深静脉血栓形成(DVT)患者血浆6-酮-前列腺素F1α(6-keto-PGF1α)及血栓素B2(TXB2)含量的影响。方法将66例DVT患者随机分为2组,治疗组38例予消栓灵,对照组28例予低分子肝素钙,2组均10 d为1个疗程,2个疗程后观察2组治疗前后6-keto-PGF1α及TXB2变化情况,统计临床疗效。结果治疗组总有效率94.74%,对照组总有效率75.00%,2组总有效率比较差异有统计学意义(P<0.05),治疗组临床疗效优于对照组。2组治疗后6-keto-PGF1α明显升高(P<0.01,P<0.05),TXB2明显下降(P<0.01,P<0.05),TXB2/6-keto-PGFlα值下降(P<0.01,P<0.05),且2组组间比较差异有统计学意义(P<0.05)。结论消栓灵可通过调节TXB2与6-keto-PGFlα之间的平衡疗治疗DVT,疗效确切。     

人骨髓间充质干细胞联合单倍体造血干细胞移植治疗急性放射病小鼠的实验研究

目的 观察人骨髓间充质干细胞（BMSC）联合单倍体相合造血干细胞（HSC）移植治疗辐射重度造血损伤小鼠的效果。方法 60只C57BL/6（H-2b）与BALB/c（H-2d）杂交第1代[CB6F1（H-2b×d）]小鼠用⁶⁰Co γ射线8.0 Gy全身照射后,按照完全随机设计分为照射对照、HSC单移植和BMSC+HSC共移植3组,每组20只。检测移植后各组小鼠存活率、外周血象、骨髓病理、骨髓细胞集落形成以及移植物抗宿主病（GVHD）评分等指标。结果 照射对照组小鼠辐照后第10天全部死于造血衰竭,平均活存时间为（7.13±0.31） d。与单移植组相比,共移植组小鼠的移植物抗宿主病（GVHD）表现和病理改变均明显减轻,GVHD评分明显降低（t=3.677、4.330、5.303、3.578,P<0.05）;共移植组的生存率和生存时间明显高于单移植组（t=3.317、5.183,P<0.05）。单移植组小鼠移植后第28天WBC（6.51±1.38）×10⁹/L、PLT（749.56±190.72）×10⁹/L、RBC（8.15±0.74）×10¹²/L、Hb（115±10.44） g/L,回升程度低于正常水平。共移植组小鼠移植后第28天WBC（12.50±2.07）×10⁹/L、PLT（968.25±216.62）×10⁹/L、RBC（9.22±1.04）×10¹²/L、Hb（137.57±14.89） g/L,回升程度接近正常水平。单移植组小鼠外周血象的回升速度和恢复水平明显低于共移植组小鼠（t=6.665、3.164、3.011、2.520,P<0.05）。移植后第7天共移植组小鼠骨髓抑制程度较单移植组轻,第28天共移植组小鼠骨髓新生造血灶多于单移植组。共移植组小鼠骨髓CFU-E、BFU-E、CFU-GM、CFU-GEMM集落计数在辐照后第7和28天均明显多于单移植组（t=3.625、2.966、3.020、3.536,P<0.05;t=4.369、4.849、5.044、4.243,P<0.05）。结论 BMSC联合单倍体造血干细胞移植能促进急性骨髓型放射病造血重建及植入,降低GVHD的发生,提高移植成功率。     

调压活络方治疗H型高血压的疗效及机制研究

目的:观察调压活络方治疗H型高血压的疗效,初步探讨其机制。方法:将2015年3月至2016年2月就治的H型高血压患者94例采用随机数字表法分为2组,每组47例,对照组给予依叶片口服,1片/d;治疗组给予依叶片+调压活络方治疗,1剂/d,持续治疗2个疗程。观察2组治疗效果,检测血管活性物质、血液流变学、氧化应激指标及肾素-血管紧张素Ⅱ-醛固酮(Renin AngiotensinⅡAldosterone System,RAAS)系统变化。结果:1)治疗组治疗后除了气短、乏力、善忘积分外,其余症候积分及总分下降幅度均大于对照组,差异有统计学意义(P0.05);2)治疗后治疗组日间收缩压、舒张压及晚间收缩压、舒张压下降幅度大于对照组,差异有统计学意义(P0.05);2组治疗后高同型半胱氨酸(Homocysteine,Hcy)浓度比较差异无统计学意义(P0.05);3)治疗组治疗后RA、AngⅡ低于对照,差异有统计学意义(P0.05);4)2组治疗后丙二醛(Malondialdehyde,MDA)、髓过氧化物酶(myeloperoxidase,MPO)、氧化物歧化酶(Superoxide Dismutase,SOD)与治疗前比较差异无统计学意义(P0.05);5)治疗组治疗后前列环素(Prostacyclin,PGI2)低于对照组,而血栓素(Thromboxane A2,TXA2)高于对照组,差异有统计学意义(P0.05);6)治疗组治疗后血小板活化因子、血小板凝聚率、全血黏度高切均低于对照组,差异有统计学意义(P0.05)。结论:调压活络方可有效控制H型高血压患者血压,改善症状,值得临床推广适用;其机制可能与调节RAAS系统、保护内皮细胞及减少血液黏稠度有关。     

Gallbladder gangrene after percutaneous vertebroplasty,an uncommon presentation of vascular complication: a case report and analysis of the causes

We present a case of an 81-year-old man with gallbladder gangrene after percutaneous vertebroplasty (PV) that was successfully treated via laparoscopic cholecystectomy (LC). The patient underwent multilevel, thoracic PV for painful osteoporotic compression fractures. PV performed at the T6 level was complicated by severe abdominal pain owing to direct embolization of the right T6 segmental artery with penetration of bone cement into the radicular artery beneath the pedicle. Cement leakage, especially arterial embolization of cement into the general circulation, is a known potential complication following PV. Serious complications related to PV augmentation procedures, such as vertebroplasty and kyphoplasty, are rare and most often result from local cement leakage or venous embolization. Combined with this case report, we reviewed the literature regarding the unusual occurrence of direct arterial cement embolization during PV and analyzed the causes to alert clinicians to this potentially rare vascular complication.     

Inhibited interleukin 35 expression and interleukin 35–induced regulatory T cells promote type II innate lymphoid cell response in allergic rhinitis

BackgroundInterleukin (IL)-35 and IL-35–producing regulatory T cells (iTr35) have been reported to inhibit T_H2 response in allergic rhinitis (AR). However, its effects on type II innate lymphoid cells (ILC2) are not well characterized.ObjectiveTo investigate the effect of IL-35 on ILC2 in AR.MethodsA total of 25 patients with AR and 20 controls were recruited. The expression and regulation of IL-35 receptor in ILC2 were analyzed by real-time polymerase chain reaction. The effect of IL-35 on ILC2 differentiation and cytokine production was analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. In addition, iTr35 were cocultured with ILC2 to explore the effect of iTr35 on ILC2. The AR mice models were also established to confirm the role of IL-35 in vivo.ResultsThe patients with AR had decreased IL-35 expression and iTr35 proportion and increased ILC2 and type II cytokines compared with the controls. Notably, IL-35 inhibited ILC2 differentiation and type II cytokine production by regulating IL-12Rβ2 and gp130. IL-35 promoted the inducible costimulatory molecule expression by iTr35 and the inducible costimulatory molecule ligand expression by ILC2. IL-35–treated mice with AR presented decreased frequency and function of nasal ILC2.ConclusionIL-35 inhibited ILC2 responses directly or through mutual contact between iTr35 and ILC2 in AR, suggesting that IL-35 may be used as a potential treatment target in AR.     

Rapid characterization of the chemical constituents of Sanhua decoction by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke. In our study, a rapid, swift and straightforward analytical method with the help of UHPLC-FT-ICR-MS/MS was successfully developed for the first time to separate and identify the chemical constituents of Sanhua decoction. Chromatography was performed on a Universal XB C₁₈ column (150 mm × 2.1 mm, 1.8 μm) using a mobile phase containing 0.1% formic acid–water (A) and acetonitrile (B). A total of 137 compounds in Sanhua decoction were identified or tentatively characterized. The findings revealed the fact that Sanhua decoction mainly contains flavonoids (in Aurantii fructus immaturus and Rheum palmatum L.), anthraquinones (in Rheum palmatum L.), coumarins (in Notopterygii Rhizoma Et Radix), phenylpropanoid glycosides, alkaloids and lignans (in Magnoliae Officmalis Cortex), which made up the key ingredients existing in Sanhua decoction. This study is hoped to be meaningful for the characterization of components in other traditional Chinese medicines, and lay the foundation for research on the pharmacology of Sanhua decoction.

Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke.     

Synthesis and photophysical studies of self-assembled multicomponent supramolecular coordination prisms bearing porphyrin faces

Multicomponent self-assembly, wherein two unique donor precursors are combined with a single metal acceptor instead of the more common two-component assembly, can be achieved by selecting Lewis-basic sites and metal nodes that select for heteroligated coordination spheres. Platinum(II) ions show a thermodynamic preference for mixed pyridyl/carboxylate coordination environments and are thus suitable for such designs. The use of three or more unique building blocks increases the structural complexity of supramolecules. Herein, we describe the synthesis and characterization of rectangular prismatic supramolecular coordination complexes (SCCs) with two faces occupied by porphyrin molecules, motivated by the search for new multichromophore complexes with promising light-harvesting properties. These prisms are obtained from the self-assembly of a 90° Pt(II) acceptor with a meso-substituted tetrapyridylporphyrin (TPyP) and dicarboxylate ligands. The generality of this self-assembly reaction is demonstrated using five dicarboxylate ligands, two based on a rigid central phenyl ring and three alkyl-spaced variants, to form a total of five free-base and five Zn-metallated porphyrin prisms. All 10 SCCs are characterized by ³¹P and ¹H multinuclear NMR spectroscopy and electrospray ionization mass spectrometry, confirming the structure of each self-assembly and the stoichiometry of formation. The photophysical properties of the resulting SCCs were investigated revealing that the absorption and emission properties of the free-base and metallated porphyrin prisms preserve the spectral features associated with free TPyP.The use of sunlight is ubiquitous as the input for carbon-neutral, renewable energy schemes (1). Every strategy that relies on solar energy conversion, ranging from direct conversion to electricity in photovoltaics (2) to the generation of fuels via electrocatalysis (3), photoanode (4, 5), or photocathode devices (6), or photocatalysis (7) requires that photons be absorbed by a molecule or material as the first step in providing the driving force for subsequent transformation. Natural systems have evolved light-harvesting complexes, comprising multiprotein ensembles embedded with pigment molecules to enhance photon absorption for photosynthesis (8). These pigment-rich sites are arranged such that excitation of a distal chromophore will ultimately result in energy transfer to a reaction center via a series of migration and transfer processes (9). Strategies to replicate natural light-harvesting complexes necessarily demand the organization of multiple chromophores (10), a requirement that makes self-assembly and supramolecular chemistry particularly well suited for such efforts (11–13). As such, a variety of approaches have been applied toward the development of new materials that exhibit broadband absorption and efficient energy transfer (14–16). The subsequent studies of such materials span investigations of the fundamental science behind energy migration and transfer, to practical applications in devices.Many of these designs incorporate porphyrin-based molecules (17–22), a pigment that is related to the ubiquitous chlorophyll found in natural light-harvesting systems (23). Porphyrins possess characteristic structural and photophysical properties that make them well suited for adaptation into artificial designs, not only for solar energy conversion but also for applications in photodynamic therapy (24–29), enzyme mimics (30–32), catalysis (33–37), and molecular electronic devices (38, 39). In the context of solar energy, many multiporphyrin light-harvesting designs have been developed ranging from organic polymers, metal-organic frameworks, and supramolecular ensembles. Porphyrins are remarkable precursors for incorporation into supramolecular designs due to their established syntheses that facilitate well-defined functionalization, the commercial availability of simple variants, and their ability to accommodate a wide range of metal ions, thus unlocking suites of complexes for a given design (40, 41). The absorption spectra of porphyrin molecules are dominated by so-called Soret bands in the 380- to 500-nm range, accompanied by a set of weaker, but still considerably intense Q bands in the 500- to 700-nm range. Because these bands result in the absorption of visible photons, the attractiveness of porphyrins in solar conversion schemes becomes apparent. Furthermore, the emission wavelengths of a given porphyrin may align with its Q-band absorption, providing the spectral overlap that is required for efficient energy migration.Although extended structures such as polymers and metal-organic frameworks can organize similar numbers of pigments as are found in natural systems (42–47), their study can be complemented by relatively smaller supramolecules that facilitate characterization and study, preserving attractive properties such as solubility and facile tenability (48, 49). As such, coordination-driven self-assembly has been used to explore porphyrin-based supramolecular coordination complexes in traditional one- or two-component schemes (50-53).The recent development of new strategies for multicomponent self-assembly has provided a method to increase the structural complexity of chromophoric supramolecular coordination complexes (SCCs) wherein three or more building blocks can assemble into a single thermodynamically favored product (54, 55). One method to achieve multicomponent assembly is to identify metal nodes and ligands that prefer heteroligated coordination spheres, thus avoiding statistical mixtures of products. For example, when Pt(II) ions are combined with pyridyl and carboxylate-based ligands, mixed Pt-N,O coordination environments are formed exclusively (56). This method has been used recently to construct a variety of 2D and 3D SCCs and also provides the basis for supramolecule-to-supramolecule transformation, wherein a Pt-pyridyl SCC may be mixed in the proper stoichiometry with a Pt-carboxylate SCC to quantitatively furnish a third multicomponent SCC (56). Furthermore, traditional two-component assembly typically requires the use of rigid building blocks so as to maintain the angularity and directionality that determines the structural outcome of a reaction. In contrast, multicomponent approaches also permit the use of structurally ambiguous ligands, provided the remaining building blocks enforce a proper degree of directionality. As such, alkyl-based building blocks may be used in such self-assembly schemes, despite the fact that rotation about the carbon–carbon bonds means that a single orientation of the coordination vectors is not maintained (57).We report here a unification of many recent themes of coordination-driven self-assembly: the formation of multichromophore complexes as motivated by the need for new light-harvesting materials, multicomponent self assembly using Pt(II) heteroligation, and alkyl-based dicarboxylates in the formation of 3D prisms that demonstrate a hitherto-underexplored class of building blocks for coordination-driven self-assembly. The synthesis and characterization of a suite of 10 prisms is described, thus demonstrating the structural modularity and tunability that is possible through self-assembly approaches. Five prisms are formed containing free-based tetrapyridyl porphyrin, with the remaining five being their Zn-containing analogs. The absorption and emission properties of the SCCs are evaluated and compared with free tetrapyridylporphyrin (TPyP) revealing that, upon incorporation into prisms, the photophysical properties remain largely unchanged. In addition, the solubility of the prisms far exceeds that of the parent TPyP, indicating that coordination-driven self-assembly may be a powerful method to optimize the physiochemical characteristics of new light-harvesting materials.     

Photoelectrical properties of graphene/doped GeSn vertical heterostructures

GeSn is a group IV alloy material with a narrow bandgap, making it favorable for applications in sensing and imaging. However, strong surface carrier recombination is a limiting factor. To overcome this, we investigate the broadband photoelectrical properties of graphene integrated with doped GeSn, from the visible to the near infrared. It is found that photo-generated carriers can be separated and transported with a higher efficiency by the introduction of the graphene layer. Considering two contrasting arrangements of graphene on p-type and n-type GeSn films, photocurrents were suppressed in graphene/p-type GeSn heterostructures but enhanced in graphene/n-type GeSn heterostructures when compared with control samples without graphene. Moreover, the enhancement (suppression) factor increases with excitation wavelength but decreases with laser power. An enhancement factor of 4 is achieved for an excitation wavelength of 1064 nm. Compared with previous studies, it is found that our graphene/n-type GeSn based photodetectors provide a much wider photodetection range, from 532 nm to 1832 nm, and maintain comparable responsivity. Our experimental findings highlight the importance of the induced bending profile on the charge separation and provides a way to design high performance broadband photodetectors.

The photoelectrical properties of graphene integrated with doped GeSn have been investigated and a high performance broadband photodetection can be achieved by integration of graphene with n-type GeSn.