针刺配合白木香根治疗颈性眩晕50例

目的 :探讨针刺、针刺配合闽东青草白木香根治疗颈性眩晕。方法 :对 10 0例以眩晕为主要症状的颈椎病进行随机分组治疗 ,5 0例针刺治疗 ,另 5 0例针刺配合白木香根治疗。结果 :针刺组有效率80 % ,针药组有效率 94 % ,经统计学处理 ,P <0 .0 5 ,有显著差异。结论 :针刺治疗颈性眩晕是重要的治疗方法 ,但针刺与服药配合优于针刺组 ,说明白木香根有促进病情康复。     

Higher gastric cycloxygenase-2 expression and precancerous change in Helicobacter pylori-infected relatives of gastric cancer patients.

PURPOSE: This study was conducted to determine whether relatives of gastric cancer patients (GCF) showed greater gastric cycloxygenase-2 (COX-2) expression or a greater incidence of precancerous lesions after Helicobacter pylori infection and whether H. pylori eradication could reduce COX-2 expression. EXPERIMENTAL DESIGN: Three hundred subjects were enrolled in this study: half were relatives of 50 H. pylori-infected gastric cancer patients, and half were relatives of 50 H. pylori-infected duodenal ulcer (DU) patients (controls). Each relative underwent endoscopy to detect H. pylori infection and related gastric histology. One hundred and twenty GCFs were found to have H. pylori infection. After H. pylori eradication, 90 of the 120 GCFs were followed up with annual endoscopy examinations over the next 2 years. Gastric COX-2 intensity in all of the specimens collected from these patients was immunochemically stained and graded from 0 to 4. RESULTS: H. pylori infection, gastric atrophy, and intestinal metaplasia (IM) were more prevalent in GCFs than in relatives of H. pylori-infected patients with DUs (P < 0.05). H. pylori-infected GCFs also showed a greater COX-2 intensity than H. pylori-infected relatives of patients with DUs (89.1% versus 62.7%, P < 0.001; relative risk: 4.9; 95% confidence interval: approximately 2.34-10.29). Among the H. pylori-infected GCFs, COX-2 intensity correlated with atrophy and IM (P < 0.001). After H. pylori eradication, gastric COX-2 expression disappeared only in those relatives without IM (P < 0.001). CONCLUSIONS: GCFs are more likely to show greater gastric COX-2 expression and a higher incidence of precancerous lesions after H. pylori infection than the relatives of H. pylori-infected patients with only DUs. H. pylori eradication can reverse gastric COX-2 expression in patients without IM but not in patients with IM.     

逆行肝切除治疗难切性肝癌244例报道

目的　逆行肝切除是治疗难切性肝癌的有效方法 ,本文报道了 2 44例临床经验。方法　常规肝切除方法切除困难的 2 44例巨大、显露困难或下腔静脉受累的肝癌采用逆行切肝法结合血管外科技术予以切除 (A组 ) ,同期临床特征类似的 31例肝癌采用常规切肝法切除 ,作为对照 (B组 )。结果　两组均无手术死亡 ,A组与B组相比 ,术中出血量较少 ( 12 90± 998ml比 2 2 86± 136 3ml)、术后胸水发生率 ( 2 6 /2 44比 10 /31)、腹水发生率 ( 72 /2 44比 19/31)、中度到重度黄疸率 ( 14 /2 44比 5 /31)、手术区积液率 ( 17/2 44比 7/31)、膈下感染率 ( 3/2 44比 1/31)、胆漏发生率 ( 2 /2 44比 1/31)、切口感染率 ( 3/2 44比 1/31)以及ALT恢复时间 ( 13.8± 5 .1天比 18.9± 8.9天 )均较低 ,差别具有统计学意义 (P <0 .0 1)。结论　对于难切性肝癌而言 ,逆行肝切除是安全有效的手术方法。     

包括腔静脉旁部的肝右尾叶及全尾叶切除

目的 探讨并总结包括腔静脉旁部肝尾叶切除的方法和经验。方法 分别采用右后途径和左侧途径行右尾叶和全尾叶切除；前者附加部分右后叶切除，后者可为单独全尾叶切除或附加左外叶或左半肝切除。结果 成功施行包括腔静脉旁部的肝尾叶切除13例，其中右尾叶切除7例，全尾叶切除6例；全组无手术死亡，术中、术后均无严重病发症发生；术后平均失血量为896.15ml，平均肝门阻断时间为25.40min，术后平均住院12.38天。结论 虽然解剖关系复杂，切除包括腔静脉旁部的肝尾叶安全可行。     

TELOMERASE ACTIVITY OF FIBROBRONCHOSCOPIC BRUSHING CELLS IN NON－SMALL CELL LUNG CANCER

Telomerase is a ribonucleoprotein enzyme that uses its own integral RNA as a template for synthesis of the telomeric DNA. The cells containing thisenzyme can escape from natural telomeric shortening and acquire infinite growth potentiality. Findings in recent studies showed that telomerase was activated in almost all malignant tumor tissues which suggests that the activation of telomerase is the critical procedure in oncogenesis. We studied telomerase activity in bronchial exfoliated cells f…     

人单核巨噬细胞泡沫化抑制剂筛选模型的建立及应用

目的: 建立人单核巨噬细胞泡沫化抑制剂筛选模型,筛选得到可抑制细胞泡沫化的抑制剂.方法: U937单核细胞经100 nmol·L-1佛波酯(PMA)诱导72 h分化为巨噬细胞后,换无血清培养液于96孔板中,每毫升含巨噬细胞1×106个,每孔再加入80 mg·L-1氧化的低密度脂蛋白(ox-LDL),37 ℃培养48 h,建立单核巨噬细胞泡沫化模型.利用微生物发酵液,或单一的化合物样品与其共孵育,油红.染色后观察细胞胞内变化,寻找对泡沫细胞形成有抑制作用的样品.利用基因工程技术表达的人清道夫受体A类II型的胞外部分,在本模型中可抑制巨噬细胞泡沫化的形成,进一步验证了模型的可行性.结果: 从2000 个微生物发酵液中筛选到6株微生物发酵液为阳性,从10个化合物中发现一个有抑制巨噬细胞泡沫化活性的新化合物.结论: 本模型可用于细胞泡沫化抑制剂的高通量筛选.     

加味酸枣仁胶囊的研制

目的:优选加味酸枣仁胶囊的制备工艺.方法:以酸枣仁皂苷A含量为指标,考察提取溶媒、加溶媒量、提取时间三因素对提取效果的影响;采用固定圆锥底法考察粉体的流动性;选择测定酸枣仁皂苷A的含量作为控制本品的质量标准.结果:酸枣仁最佳提取工艺即用70%乙醇提取2次,第1次2 h,第2次1 h,用乙醇量均为8倍药材量;粉末水分控制在3.01%流动性好,休止角平均值为28.2°;该胶囊含酸枣仁皂苷A不得低于11.00mg/粒.结论:上述实验结果可为加味酸枣仁胶囊的制备提供实验依据.     

抗体夹心酶联免疫吸附法测定重组E.coli L-门冬酰胺酶研究

用重组E.coliL-门冬酰胺酶免疫家兔，DEAE-纤维素柱层析纯化IgG，采用二步戊二醛交联法将辣根过氧化物酶标记抗体，建立抗体夹心酶联免疫吸附法，测定大鼠血浆中重组E.coliL-门冬酰胺酶浓度。本测定方法的线性范围为1～64U/L，灵敏度为0.4U/L。建立的抗体夹心酶联免疫吸附法测定大鼠血浆中重组E.coliL-门冬酰胺酶具有良好的精密度、回收率、灵敏度和特异性。