Analyses of mutation and loss of heterozygosity of coding sequences of the entire transforming growth factor beta type II receptor gene in sporadic human gastric cancer

Mutations in the transforming growth factor beta type II receptor (TGFbetaRII) gene have been detected in several human cancer types exhibiting microsatellite instability. Using intron primers previously reported for examination of the entire coding region of the TGFbetaRII gene, 29 sporadic gastric cancers were screened with non-radioactive single strand conformation polymorphism and subsequent DNA sequencing analysis. Mutations of the TGFbetaRII gene were detected in three out of 29 tumors (10%). Two cases showed deletions in a polyadenine tract in both alleles and was positively associated with replication error. One case had an insertion of GA dinucleotide sequence in one allele. Mutations of the TGFbetaRII gene were restricted to exon 3 and other coding regions were not affected. Loss of heterozygosity was detected by analyzing a polymorphic site in intron 2. Three out of nine (33%) informative cases, which were all of intestinal type and advanced cases, showed loss of heterozygosity but neither TGFbetaRII mutation nor replication error was found in these cases. Immunoreactivity of TGFbetaRII in tumor tissues was reduced to a different extent in the gastric cancer with genetically abnormal transforming growth factor. Although the numbers studied are small, homozygous (A)10 deletion or loss of heterozygosity of TGFbetaRII is involved in tumorigenesis and progression of at least some part of sporadic gastric cancer.      

RER phenotype and its associated mutations in familial gastric cancer

To clarify the genetic background of gastric cancer, we collected 28 familial gastric cancers (FGCs) with reference to the Amsterdam criteria in hereditary non-polyposis colorectal cancer (HNPCC) and investigated the frequency of replication error (RER) at six microsatellite loci and frameshift mutations in its related genes in these tumors. RER was detected in seven (25%) of the 28 gastric cancers. Five (18%) cases showed RER at more than two loci. The apparent increased incidence of RER in FGC was not detected compared with that reported in sporadic gastric cancers previously. Among four cases with RER at more than three loci, frameshift mutations in the (A)8 track of the hMSH3 gene were detected in all the four cases and mutations in the (A)10 track of the transforming growth factor-beta type II receptor (TGF-beta RII) gene were detected in the three of them. Histologically, three of the four cases were of the intestinal type, and the other one was the diffuse type. No mutation was detected in the (C)8 and (GT)3 tracks of the hMSH6 and TGF-beta RII genes respectively. These results indicate that the acquisition of the RER phenotype equally influences the gastric carcinogenesis of both sporadic and familial cases, and that the majority of FGC is pathogenetically distinct from HNPCC.      

骨转移瘤的放射治疗

目的探讨关于骨转移瘤常规分割放疗组与低分割放疗组的止痛效果。方法４８例病人，２８例采用常规分割，ＤＴ２００ＣＧＹ／次，每周５次，ＤＴ３０００～５０００ＣＧＹ；２０例采用低分割照射，ＤＴ４００～５００ＣＧＹ／次，每周２～３次，ＤＴ２５００～３０００ＣＧＹ。结果放疗起到了明显的止痛作用，常规分割放疗组与低分割放疗组止痛效果大致相同，无统计学意义（Ｐ＞０．０５）。结论放疗原则应根据病情及预计生存期长短来决定。对一般情况好，预计生存期长的病人应采取积极的治疗，给予大剂量长疗程的常规分割，而对于那些病情相对较重，行动又不方便，无望长期生存的病人，应采取低分割照射，且见效快，同样起到止痛作用     

可变数串联重复序列检测异基因外周血造血干细胞移植后植？…

目的 从血型、染色体核型、ＤＮＡ可变数串联重复序列（ＶＮＴＲ）几个方面进行对比研究以更好地评估异基因移植后的植入状态及其与疾病复发的关系。方法 采用聚合酶链反应（ＰＣＲ）方法扩增Ｄ１７Ｓ３０、Ｄ１Ｓ８０、ＡｐｏＢ３个不同位点的多态性,对１５例白血病患者异基因外周血造血干细胞移植（ａｌｌｏ－ＰＢＳＣＴ）后的嵌合状态进行检测,对其中７例异性间移植和３例血型不合移植又分别进行了染色体核型分析和全套血型分     

The effect of Rhodiola capsules on oxygen consumption of myocardium and coronary artery blood flow in dogs]

It has been proved that Rhodiola Capsules can obviously decrease the oxygen consumption of myocardium and oxygen consumption index in anesthetic dogs, decrease the coronary artery resistance, but have no marked effect on the coronary artery blood flow. The Capsules also have the function of decreasing blood presure and slowing heart rate.     

Chemical constituents of Conyza blinii Lévl.]

Five compounds were isolated from the aerial part of Conyza blinii. On the basis of physicochemical properties and spectroscopic analysis, they were identified as alpha-spinasterol, 5, 8-dihydroxy-7, 3', 4'-trimethoxyflavone, caffeic acid, quercetin and syringic acid. All these compounds were isolated from the plant of C. blinii for the first time.     

钢基Cu-5%Al激光熔覆层颗粒组织分布的定量分析

熔覆层中的颗粒相分布不仅影响材料性能，同时也是研究熔覆过程各种反应机制的主要依据。本文利用图像分析技术，定量分析了Ｑ２３５钢表面Ｃｕ５％Ａｌ激光熔覆层的富铁相颗粒分布情况。发现熔覆过程中在熔池中存在熔体对流环，熔池中的对流带动颗粒运动，是决定熔覆层颗粒分布的主要因素。     

柴蔻冲剂对慢性酒精性肝损伤大鼠抗脂质过氧化作用的实验研究

观察中药复方柴蔻冲剂（ＣＫＩ）对慢性酒精性肝损伤大鼠的防护作用。ＣＫＩ可显著提高红细胞和肝组织中超氧化物歧化酶（ＳＯＤ）活性，血与肝组织中过氧化氢酶（ＣＡＴ）、谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）的活性。显著降低血浆与肝组织中脂质过氧化终产物丙二醛（ＭＤＡ）含量，抑制氧自由基的产生，提高组织抗氧化能力。并探讨了柴蔻冲剂的量效关系。     

中西医结合治疗慢性周围动脉闭塞性疾病

慢性周围动脉闭塞性疾病（PAO）治疗较为棘手。闭塞的血管不易再通，手术适应症范围小，且术后可能再度发生闭塞[1]。因此寻求适合的保守疗法非常重要。我院自1995年3月至1997年3月治疗PAO患者35例，其中23例用中药溶栓灵配合西药前列素E（PGE1）、天普乐欣（UK）治疗，与单纯用西药治疗的12例比较，疗效尚满意，现报道如下。1临床资料根据中国中西医结合研究会周围血管病专业委员会制定的诊断和分期标准[2]，收治35例PAO患者，包括血栓闭塞性脉管炎（TAO）19例，动脉硬化性闭塞（ASO）16例。随机分为两组：①溶栓灵结合PGEI、UK…     

大蒜液室内空气消毒效果的观察

目的：观察新鲜大蒜液对室内空气喷雾的消毒效果。方法：采用自然沉降法，用4种剂量的大蒜液对室内空气进行喷雾消毒，并在不同时间段．取空气样本作细(真)菌总数比较。结果：4种剂量的大蒜液对室内空气喷雾消毒均有显著的杀菌效用。结论：用新鲜大蒜液喷雾消毒是室内空气消毒的有效方法。