细胞内抗原免疫金银染色技术的改进

为解决细胞内抗原应用免疫金银法染色时背景过重的问题,建立了甘氨酸二次阻断的处理方法,效果较好。     

EB病毒感染与中线恶性网织细胞增多症

使用一个针对ＥＢ病毒编码的小分子ＲＮＡ（ＥＢＥＲ）的寡核苷酸探针对１９例中线恶性网织细胞增多症病变组织进行了原位杂交检测，并配合免疫表型分析。结果为：（１）本组病例中１８／１９例（９４．７％）的病变组织中的异形淋巴样细胞表达Ｔ细胞分化抗原。ＣＤ３阳性１５例，ＵＣＨＬ１阳性９例，其中二者均阳性６例，但各例中的异形淋巴样细胞均未表达Ｂ细胞及组织细胞分化抗原；（２）ＥＢＥＲ原位杂交检测的阳性数高（１５／     

心脏组织工程瓣脉动流培养系统的设计与构建

自行设计和制造平面和三维立体培养室及贮液室等构件,用医用硅胶管连接;转子泵作为动力源,贮液室通气口供给5%CO2 95%空气,恒温水浴箱保持构件37℃恒温,这样组成了种植细胞与生物瓣支架复合体的脉动培养系统,并进行生物力学和生物相容性测试,为心脏组织工程瓣的体外构建提供研究器材。结果显示,该装置密闭性能好,内环境能保持37±1℃、CO2浓度5%±1%、pH值6.8～7.5;流量在0.125～6.0L/min的范围内任意调节;同种瓣膜上的内皮细胞经2周培养后扩增约10倍;瓣膜支架的细菌和霉菌培养均为阴性,说明我们构建的脉动流培养系统能有效地模拟体内脉动流场实现种植细胞在体外的增殖、重塑,为心脏组织工程瓣的体外构建提供了一种新的实验方法。     

Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels

Previous studies have shown that gap junctional channels formed from the lens connexins Cx50 (or its chicken orthologue, Cx45.6) and Cx43 exhibit marked differences in transjunctional voltage gating and unitary conductance. In the present study, we used the negatively charged dye, Lucifer Yellow (LY), to examine and compare quantitative differences in dye transfer between pairs of HeLa cells stably transfected with Cx45.6 or Cx43. Our results show that Cx45.6 gap junctional channels are three times less permeable to LY than Cx43 channels. Replacement of the N-terminus of Cx45.6 with the corresponding domain of Cx43 increased LY permeability, reduced the transjunctional voltage ( V _j) gating sensitivity, and reduced the unitary conductance of Cx45.6–43N gap junctional channels. Further experiments, using a series of Alexa probes that had similar net charge but varied in size showed that the Cx45.6–43N had a significantly higher permeability for the two largest Alexa dyes than Cx45.6. These data suggest that the N-terminus plays a critical role in determining many of biophysical properties of Cx45.6 gap junctional channels, including molecular permeability and voltage gating.     

Effect of anti-inflammatory medication on monocyte response to titanium particles

Cytokines produced by macrophages in the periprosthetic membranes surrounding joint replacements have been implicated as causal agents in osteolysis and prosthetic loosening. The present study characterizes the response of human peripheral blood monocytes to titanium particles. Monocytes were obtained from donated blood and were cultured in the presence of different-sized titanium particles. Exposure to titanium-aluminum-vanadium particles significantly changed the release of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1 (IL-1), whereas there was no significant effect on the release of prostaglandin E(2) (PGE(2)). When monocytes were cultured with particles, the titanium alloy particles induced significantly more release of TNF-alpha and less IL-1 secretion. Ciprofloxacin inhibited production of TNF-alpha, IL-6, IL-1, and PGE(2) in human monocytes exposed to titanium particles. In contrast to ciprofloxacin, indomethacin was not a potent inhibitor of TNF-alpha production but potentiated IL-6 production in titanium-stimulated monocytes. Indomethacin had no effect on the production of IL-1 and was a potent inhibitor of PGE(2) production in titanium-stimulated monocytes. Pentoxifylline had an inhibitor effect on TNF-alpha production in titanium-stimulated monocytes. Pentoxifylline potentiated IL-6 and IL-1 production in monocytes exposed to titanium particles and had a biphasic effect on the PGE(2) production. The results of this study support our hypothesis that human monocytes release bone resorption mediators after in vitro exposure to TiAlV alloy particles. The results also demonstrate the differences of bone-resorbing mediators in response to different wear particle size. The pharmacologic agents (ciprofloxacin, pentoxifylline, and indomethacin) that can modulate the release of bone resorbing mediators such as PGE(2), TNF-alpha, IL-1, and IL-6 release from human monocytes. The results help to elucidate the differences in cellular response to wear particles but may not be directly transposed to the human situation.     

102T/C polymorphism of serotonin receptor type 2A gene is not associated with schizophrenia in either Chinese or British populations

Several pieces of evidence implicate serotonin receptors in the aetiology of schizophrenia, and recently a number of studies have reported a genetic association between the 102T/C polymorphism of serotonin receptor type 2A gene and schizophrenia. Unfortunately a number of failures to replicate these positive associations in both Caucasian and Chinese populations have also been reported. We have examined the 102T/C polymorphism by PCR amplification and restriction analysis of DNA from: 202 schizophrenics and 202 controls from Shanghai; 112 schizophrenics and 224 parents from Chengdu, Cina; and 253 schizophrenics and 244 controls from the the UK. We find no evidence of association or transmission disequilibrium between the 102T/C polymorphism and schizophrenia in any of the groups we have examined. We conclude that either the original positive reports occurred by chance or any effect must be minimal, and urge caution in interpreting small positive results derived using data from different centres.     

半导体激光体外照射血管治疗仪的研制

为克服 He-Ne激光血管内照射疗法所具有的不足,我们采用830nm、500mW半导体激光器,研制了用于体外血管照射的激光治疗仪.设计了易于固定的激光输出头,并且采用单片机控制整机,提高了仪器的稳定性和可靠性.临床应用取得了令人满意的治疗效果.     

MTEC 1分泌的趋化因子引起特定亚群胸腺细胞的定向迁移

分析胸腺髓质上皮样细胞系ＭＴＥＣ１分泌的化学趋化因子对胸腺细胞亚群的趋化作用。方法以抗体加补体杀伤结合免疫磁珠及ｐａｎｎｉｎｇ法，将小鼠胸腺细胞分离纯化，获得ＣＤ４＋ＣＤ８＋（ＤＰ），ＣＤ４－ＣＤ８－（ＤＮ），ＣＤ４＋ＣＤ８－（ＣＤ４ＳＰ）及ＣＤ４－ＣＤ８＋（ＣＤ８ＳＰ）四亚群细胞，用Ｂｏｙｄｅｎ小室分析ＭＴＥＣ１┐ＳＮ对四群胸腺细胞的趋化作用。结果ＭＴＥＣ１┐ＳＮ对ＤＰ及ＣＤ４ＳＰ胸腺细胞有趋化活性（ＣＩ＝６．６±１．０及６．１±１．８）；对ＣＤ８ＳＰ细胞有中度趋化活性（ＣＩ＝３．２±１．０）；对ＤＮ趋化活性微弱（ＣＩ＝１．３±０．６）。化学趋化因子ＭＣＰ┐１纯品对ＣＤ４ＳＰ胸腺细胞显示强趋化活性（ＣＩ＝５．６），对ＤＮ胸腺细胞则无可测出趋化活性。结论ＭＴＥＣ１分泌的化学趋化因子对ＤＰ，ＣＤ４ＳＰ及ＣＤ８ＳＰ胸腺细胞有显著趋化作用，对ＤＮ胸腺细胞几乎无趋化作用。提示此类化学趋化因子有趋使胸腺发育中后期阶段的细胞向胸腺髓质区迁移和定位的作用。     

颈椎病患者经颅多普勒检查与头颅CT和脑电图的关系

应用多普勒技术对50例确诊为椎动脉型颈椎病的患者进行了检查,结果30例异常。其中25例主要表现为不同程度的双侧颈动脉血流量对称性偏低,管径及头颅CT无异常,脑电图部分改变,主要表现为低幅慢波,经治疗后症状改善,多普勒复查正常,说明CT正常不能否定脑缺性血管病的存在,CT不能代替多普勒检查,另5例主要表现为双侧颈动脉血流量不对称,其中3例为双侧血管经狭窄,CT提示全脑萎缩,脑电图呈低幅慢波;另2例CT提示左颞后顶区,右枕外侧区梗塞,脑电图及脑电地形图表现相应部位慢波灶达6～7级。CT异常者,临床治疗效果欠佳,多普勒检查和脑电图及临床症状变化不大,故多普勒检查对监测脑动力循环有一定意义。     

圆斑蝰蛇毒中性磷脂酶A2对不同种类血管条作用的比较

本文比较了福建圆斑蝰蛇毒中性磷脂酶Ａ２（ＰＬＡ２－３）对人胃网膜动脉条、兔和大鼠主动脉条的作用，表面ＰＬＡ２－３对处于静息状态和预收缩状态的人网膜动脉能诱发松弛反应，而对大鼠和兔主动脉诱发剂量依赖性收缩反应，表明该ＰＬＡ２的血管作用具有血管种类差异性。ＰＬＡ２－３对大鼠主动脉的收缩反应是内皮细胞非依赖性的，去除内皮细胞能增强ＰＬＡ２－３所诱发的收缩反应；ＰＬＡ２－３所诱发的大鼠主动脉条收缩反应可能