自主神经竞争性机制在肺叶切除术后房颤发生中的作用

目的：探讨自主神经在肺叶切除术后房颤发生中的作用。方法对281例60岁以上接受肺叶或全肺切除术的患者进行术前24 h、术后连续96 h 的动态心电图监测,对出现房颤患者术前2 h、房颤发生前60 min、30 min、5 min 的动态心电图的心率变异性指标进行分析,选取5 min 的阶段,研究心率变异性在房颤发生前4个时间点的变化趋势,分析交感神经张力与迷走神经张力在房颤发生前的变化。结果281例肺切除患者术后有48例出现房颤,发生率17．1％;房颤发生前60 min、30 min、5 min 反映迷走神经张力的指标 SDNN、rMSSD、pNN50、HF 均有明显升高,而反映交感／副交感神经平衡的指标 LF ／HF 则有逐渐降低趋势;在房颤发生前5 min 的 SDNN、rMSSD、pNN50、HF 明显升高,而 LF ／HF 比值明显降低,表明迷走神经张力明显升高,RR 间期在房颤发生前逐渐缩短。结论肺叶切除术后的房颤发生前心率变异性存在明显升高的表现,肺叶切除术后交感神经张力明显升高的背景下迷走神经张力竞争性升高可能是导致房颤发生的重要机制。     

let-7a调控自噬对缺氧状态下原发性肝癌HCCLM3细胞增殖的影响*

目的 探讨let-7a调控自噬对缺氧状态下原发性肝癌HCCLM3细胞增殖的影响。方法 将HCCLM3细胞分为常氧组、缺氧组和缺氧let-7a组,分别给予不同的处理。各组设6个平行孔,培养72 h。采用吖啶橙染液法测定HCCLM3细胞自噬率,采用MTT法检测HCCLM3细胞活力,使用流式细胞仪分析HCCLM3细胞周期G1,采用酶联免疫吸附法测定培养上清let-7a、细胞周期D1蛋白（cyc D1）和血管内皮生长因子（VEGF）水平。结果 缺氧let-7a组细胞存活率为（49.7±9.5）%,显著低于常氧组或缺氧组的（100.0±0.0）%或（119.1±13.1）%（P<0.05）,缺氧组细胞存活率显著高于常氧组（P<0.05）;缺氧let-7a组细胞周期G1期和细胞自噬率分别为（89.6±16.1）%和（49.3±7.4）%,显著高于常氧组或缺氧组的（56.3±11.0）%和（13.2±8.1）%或（69.7±14.1）%和（23.5±5.5）%（P<0.05）,缺氧组细胞周期G1期和自噬率显著高于常氧组（P<0.05）;缺氧let-7a组细胞let-7a蛋白水平为（96.1±9.3） μmol/L,显著高于常氧组或缺氧组的（33.4±7.1）μmol/L或（65.3±5.2）μmol/L（P<0.05）,cyc D1和VEGF水平分别为（72.3±8.8）μmol/L和（56.3±6.3）μmol/L,显著低于常氧组或缺氧组的（124.1±7.2）μmol/L和（114.1±5.2） μmol/L或（98.2±6.7） μmol/L和（85.2±8.1）μmol/L（P<0.05）,缺氧组let-7a蛋白显著低于常氧组（P<0.05）,cyc D1和VEGF显著高于常氧组（P<0.05）。结论 上调let-7a在肝癌HCCLM3细胞的表达能诱发细胞自噬,使得细胞在G1期往S期的分化过程发生障碍,抑制细胞在缺氧环境中的增殖,其机制可能与细胞CYC D1和VEGF表达下调有关。     

HBV颗粒的纯化及生物学活性鉴定

目的优化纯化HBV患者血清中HBV颗粒的技术方法。方法在蔗糖介质中,分别以100 000 g和70 000 g进行不连续蔗糖梯度(60%,45%,35%,25%,15%)离心5 h,离心后,分段吸取6份收集液,PCR荧光定量检测各组分HBV滴度;收集前5份收集液进行超滤,去蔗糖,PCR荧光定量检测HBV超滤液;电镜观察70 000g纯化后的HBV超微形态;70000 g纯化后的HBV颗粒感染树鼩肝细胞,检验70 000 g纯化后的HBV感染活性。结果100 000 g离心造成大量HBV在离心过程中损失,收获率只有21%,70 000g离心不仅降低了离心中的病毒损失,而且可有效对HBV和血清进行分离,收获率达到78%以上,较100 000g还提高3.7倍多。电镜可观察到纯化后病毒液中有大量的病毒颗粒;纯化后的HBV颗粒感染活性良好,可有效感染原代树鼩肝细胞。结论70 000 g不连续蔗糖梯度离心可有效分离纯化HBV病毒,较100 000 g收获率明显提高,纯化后的HBV不但具有典型的HBV特征,还具有良好的生物学感染活性。     

Effectiveness of Chinese herbal medicine in treating liver fibrosis: a systematic review and meta-analysis of randomized controlled trials

Background

The studies on the effectiveness of Chinese herbal medicines (CHM) in treating liver fibrosis (LF) were not consistent. This study aims to systematically review the effectiveness of CHM on treating LF patients.

Methods

Databases including MEDLINE, AMED, EMBASE, The Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, TCMOnline, Chinese Biomedical Literature Database, and Chinese Medical Current Contents were searched up to March 2011. Randomized controlled trials (RCTs) involving LF patients receiving CHM, Western medicine, combined CHM and Western medicine compared with placebo, Western medicine or no intervention were included. LF markers including serum hyaluronic acid (HA), laminin (LN), procollagen type III (PC-III), type IV collagen (IV-C), matrix metalloproteinase (MMP), and tissue inhibitors of metalloproteinase (TIMP) were measured as primary outcomes. Liver biochemistry, including alanine aminotransferase (ALT) and aspartarte aminotransferase (AST), and improvement of related clinical symptoms were measured as secondary outcomes. Risk of bias of allocation sequence, allocation concealment, blinding, incomplete outcome data, selective outcome reporting, and other biases were assessed.

Results

Twenty-three RCTs with 2123 participants were analyzed in subgroups of types of comparison and study quality. Fifteen studies were graded as good quality. CHM alone and combined with Western medicine showed significant improvements in HA, LN, PC-III and IV-C compared with Western medicine alone. However, there were no significant differences observed between CHM and placebo treatments.

Conclusion

The current inconclusive results in determining the effectiveness of CHM treatment on LF, due to the poor methodological quality and high heterogeneity of the studies, suggests that large RCTs using standardized Chinese medicine syndrome diagnosis and CHM formulae with longer follow-up are required for further evaluation.     

Abnormal Development at Early Postimplantation Stage in Mouse Embryos After Preimplantation Genetic Diagnosis

Preimplantation genetic diagnosis (PGD) is an established procedure for the genetic analysis of embryos. To assess the effect of the procedure on early embryonic development, we generated a murine experimental system, including mice implanted with biopsied in vitro cultured embryos, control mice implanted with in vitro cultured embryos without biopsy, and mice with naturally conceived embryos. Embryos at the 7.5‐dpc stage were isolated from all three groups and the embryo implantation rate, the survival rate of implanted embryos, and the developmental stage of surviving embryos were carefully assessed and compared among all three groups. We found the implantation rate was similar between biopsied and control group embryos (67.92% vs. 66.67%). However, the survival rate of implanted embryos in the biopsied group (49.31%) was significantly lower than that of the control (60.91%) and normal groups (96.24%) at 7.5 dpc. In addition, the survival rate of control group embryos was significant lower than that of normal group embryos. Classification of the precise developmental stages of randomly selected live implanted embryos at 7.5 dpc revealed no differences among the three groups. Our results indicate that blastomere biopsy does not adversely affect embryo implantation. The PGD procedure, in particular blastomere biopsy, increases the rate of embryo death at 4.5–7.5 dpc, but does not affect the development of surviving 7.5 dpc embryos. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.     

伊文思蓝检测急性高眼压后大鼠血-视网膜屏障的变化

目的:检测急性高眼压后大鼠血-视网膜屏障(BRB)的改变.方法:大鼠随机分为正常对照组、实验对照组和实验组（急性高眼压模型）.动物分别存活3h、12 h、1d、3d、7d,处死前注射伊文思蓝(EB),共聚焦显微镜检测视网膜铺片和切片中EB的分布;用分光光度计定量视网膜EB的含量.结果:在急性高眼压后视网膜中可见,EB红...     

芦丁对丙烯酰胺致大鼠坐骨神经髓鞘损伤的保护作用

目的 通过观察芦丁(Rut)对丙烯酰胺(ACR)染毒大鼠坐骨神经髓鞘结构、髓鞘碱性蛋白(MBP)及髓鞘相关糖蛋白(MAG)表达的变化,探讨Rut对ACR致大鼠坐骨神经髓鞘损伤的保护作用.方法 成年SD雄性大鼠36只,随机分为4组:对照组(control,给予ddH2O)、20 mg/kg ACR染毒组(ACR)、100...     

手部肌腱损伤与修复:737例流行病学因素分析

背景:目前国内外针对手部肌腱损伤所开展的流行病学调查鲜有报道.在新疆范围内,屈伸指肌腱损伤的患者呈逐年上升趋势,但对其受伤、治疗乃至预后较完整的流行病学调查极为罕见.目的:分析手部肌腱损伤患者的流行趋势及预后分布特征,了解手部肌腱损伤患者的预后功能恢复,有针对性地开展人群干预,提高干预效果,探讨屈伸指肌腱损伤因素与预后...     

Association of IMMP2L deletions with autism spectrum disorder: A trio family study and meta‐analysis

IMMP2L, the gene encoding the inner mitochondrial membrane peptidase subunit 2‐like protein, has been reported as a candidate gene for Tourette syndrome, autism spectrum disorder (ASD) and additional neurodevelopmental disorders. Here we genotyped 100 trio families with an index proband with autism spectrum disorder in Han Chinese population and found three cases with rare exonic IMMP2L deletions. We have conducted a comprehensive meta‐analysis to quantify the association of IMMP2L deletions with ASD using 5,568 cases and 10,279 controls. While the IMMP2L deletions carried non‐recurrent breakpoints, in contrast to previous reports, our meta‐analysis found no evidence of association (P > 0.05) between IMMP2L deletions and ASD. We also observed common exonic deletions impacting IMMP2L in a separate control (5,971 samples) cohort where subjects were screened for psychiatric conditions. This is the first systematic review and meta‐analysis regarding the effect of IMMP2L deletions on ASD, but further investigations in different populations, especially Chinese population may be still needed to confirm our results.     

Inhibitory effects of erythromycin on wear debris-induced VEGF/Flt-1 gene production and osteolysis

Objectives  A highly vascularized and inflammatory periprosthetic tissue augments the progress of aseptic loosening, a major clinical problem after total joint replacement. The purpose of this study is to investigate the effect of erythromycin (EM) on ultra high molecular weight polyethylene (UHMWPE) particle-induced VEGF/VEGF receptor 1 (Flt-1) gene production and inflammatory osteolysis in a mouse model. Methods  UHMWPE particles were introduced into established air pouches on BALB/c mice, followed by implantation of calvaria bone from syngeneic littermates. EM treatment started 2 weeks after bone implantation (5 mg/kg day, i.p. injection). Mice without drug treatment as well as mice injected with saline alone were included. Pouch tissues were harvested 2 weeks after bone implantation. Expression of VEGF, Flt-1, RANKL, IL-1, TNF and CD68 was measured by immunostain and RT-PCR, and implanted bone resorption was analyzed by micro-CT (μCT). Results  Exposure to UHMWPE induced pouch tissue inflammation, increase of VEGF/Flt-1 proteins, and increased bone resorption. EM treatment significantly improved UHMWPE particle-induced tissue inflammation, reduced VEGF/Flt-1 protein expression, and diminished the number of TRAP⁺ cells, as well as the implanted bone resorption. Conclusion  This study demonstrated that EM inhibited VEGF and Flt-1 gene expression. The molecular mechanism of EM action on VEGF/Flt-1 signaling-mediated osteoclastogenesis warrants further investigation.