γ—辐射对T淋巴细胞环核苷酸昼夜节律的毒效应

测定了小鼠外周血Ｔ淋巴细胞中ｃＡＭＰ和ｃＧＭＰ含量及其比值的昼夜节律各峰值时相分别位于１２：２０时、０１：１２时和１３：０４时,经５．０Ｇｙγ－射线照射后,ｃＡＭＰ含量升高,ｃＧＭＰ一降低,ｃＡＭＰ／ｃＧＭＰ比值增大。而０．５Ｇｙ照射引起比值下降。这些变化在０４：００时较为明显,而在１２：００时则观察不到,表现出明显的昼夜时相差异。另一方面,照射后ｃＡＭＰ／ｃＧＭＰ比值节律的峰值位相发生延迟或提前     

直肠电刺激对上运动神经元损伤后痉挛状态的疗效研究

研究直肠电刺激（RPES）对上运动神经元损伤后痉挛状态的疗效。对10例脊髓损伤（SCI）后痉挛患者,10例偏瘫后痉挛患者进行RPES治疗,在治疗前及治疗后1h,对SCI患者双下肢、偏瘫患者患侧下肢进行康复评分和步态、步行能力观察。结果：经RPES治疗后,SCI患者下肢Ashworth评分、Clonus评分显著降低,患者出现步态改善和步行能力提高,偏瘫患者患侧下肢康复评分无显著变化。采用RPES治疗,对降低SCI后痉挛状态有较好的疗效,对偏瘫后痉挛状态无明显治疗作用。     

化疗药诱导卵巢癌细胞凋亡的初步探讨

目的 观察人体内化疗药诱导卵巢癌细胞凋亡及其规律性。方法 采用末端脱氧核苷酰的转移酶方法,对９例卵巢癌腹水患者行腹腔化疗,观察不同时间腹水中卵巢癌细胞凋亡的动态变化,并应用免疫组化法动态检测腹水中肿瘤细胞增殖及Ｂｃｌ－２,Ｂａｘ基因表达。结果 发现化疗后,肿瘤细胞凋亡多在化疗后４８小时达高峰,与化疗前有显著差异（Ｐ〈０．０５）,至１２０小时,凋亡逐渐下降,部分病例降至治疗前水平,化疗后腹腔内肿瘤细     

腹腔镜子宫切除34例临床分析

目的:探讨腹腔镜下子宫切除的手术方法。方法:选择子宫肌瘤31例及子宫腺肌症3例为手术对象。8例作子宫全切除,26例为子宫次全切除。以电刀凝切方法分离子宫旁韧带,子宫的游离可达Ⅲ～Ⅳ级。结果:31例顺利完成手术,手术成功率91.2％。未发生邻近脏器损伤。结论:腹腔镜子宫切除手术创伤小,但难度较常规开放手术大,预防术中出血与邻近脏器损伤是手术成功的关键。     

新型常规潜艇长航60昼夜对艇员体能耐力及心理工效的影响

目的;探讨新型常规潜艇长航６０昼夜对艇员视力听力,心肺功能、体能耐力和心理工效等的影响。方法：通过临床体检,特殊检查观察长航艇员生理和心理方面的变化。结果：长航艇员视力和听力下降,心率加快,心脏搏出量减少,用力肺活量及最大通气量减少,体能耐力和工作能力下降。结论：潜艇长航对艇员视力听力,心肺功能,体能耐力,工作能力和心理工效均有一定程度的影响。     

肺癌患者血清与支气管肺泡灌洗液四项放免肿瘤指标同步检测的结果分析

对３８例肺癌患者的血清和支气管肺泡灌洗液（ＢＡＬＦ）同步进行ＣＥＡ、ＣＡ－５０、ＡＦＰ、ＳＦ的检测。结果显示：肺癌ＢＡＬＦ的ＣＥＡ浓度测值７３％明显高于血清中ＣＥＡ浓度;ＣＡ－５０浓度测值６８％高于血清;ＳＦ９２％明显低于血清中测值;而ＡＦＰ在肺癌患者无论血清及灌洗液中,阳性率均低于７％,但仍是ＢＡＬＦ的浓度测值敏感于血清。提示支气管肺泡灌洗液的ＣＥＡ、ＣＡ－５０与血清的同步检测是目前肺癌早期诊断的主要优选方法之一。     

The eye in epidermolysis bullosa.

AIMS: To describe the ophthalmic findings in a large cohort of epidermolysis bullosa (EB) patients managed in one large specialist centre. METHODS: A case note review of consecutive patients seen at Great Ormond Street Children's Hospital. Data on the dermatological disease, ophthalmic history, and examination were collected and coded onto a data sheet. RESULTS: 181 patients: 50 (28%) simplex EB; 15 (8%) junctional EB; 28 (15%) autosomal dominant dystrophic EB; 72 (40%) autosomal recessive dystrophic EB; nine patients (5%) with dystrophic EB whose inheritance could not be ascertained; and seven cases (4%) of EB that could not be classified. Ocular problems were found in 12% (n = 6) of simplex patients and 40% (n = 6) of those with junctional disease. One patient (of 28) in the autosomal dominant dystrophic group had ocular involvement and 51% (37/72) of patients in the autosomal recessive dystrophic group had ophthalmic complications: corneal (25/72), lid ectropions (3/72), lid blisters (5/72), and symblepharon (3/72). CONCLUSION: Ophthalmic complications are common in EB overall but the incidence varies widely with subtype. Ophthalmic complications are the most severe in the dystrophic recessive and junctional subtypes where there is a need for extra vigilance. The major treatment modality was use of ocular lubricants.     

Bicyclic tripeptide mimetics with reverse turn inducing properties

Analogues of the hypertensive octapeptide angiotensin II, comprising novel constrained 5,8-bicyclic and 5,9-bicyclic tripeptide units adopting nonclassical beta-turn geometries, as deduced from theoretical conformational analysis, have been synthesized. Spontanous bicyclization upon acid-catalyzed deprotection of a model peptide, encompassing a protected omega-formyl alpha-amino acid in position 5 and cysteine residues in positions 3 and 7, revealed a strong preference for bicyclization toward the C-terminus. The bicyclic thiazolidine related angiotensin II analogues synthesized exhibited no affinity for the angiotensin II AT1 receptor.     

Angiotensin II analogues encompassing 5,9- and 5,10-fused thiazabicycloalkane tripeptide mimetics

A simple experimental procedure on solid phase for the construction of new tripeptidic 5,9- and 5,10-fused thiazabicycloalkane scaffolds that adopt beta-turns has been developed. This N-terminal-directed bicyclization, relying on masked aldehyde precursors derived from glutamic acid as key building blocks, provides a complement to the related bicyclization previously reported, where an aspartic acid-derived precursor was employed to induce cyclization toward the C-terminal end of the peptide. Thus, the regioselectivity of the bicyclization can be altered simply by varying the chain length of the incorporated aldehyde precursor. Four analogues of the hypertensive octapeptide angiotensin II, comprising the new scaffolds in the 3-5- and 5-7-positions, were synthesized. One of these conformationally constrained angiotensin II analogues exhibited AT(1) receptor affinity (K(i) = 750 nM). Results from theoretical conformational analysis of model compounds of the bicyclic tripeptide mimetics are presented, and they demonstrate that subtle differences in geometry have a strong impact on the affinity to the AT(1) receptor.