全文获取类型
收费全文 | 14389篇 |
免费 | 1350篇 |
国内免费 | 1044篇 |
专业分类
耳鼻咽喉 | 122篇 |
儿科学 | 154篇 |
妇产科学 | 162篇 |
基础医学 | 1684篇 |
口腔科学 | 187篇 |
临床医学 | 1886篇 |
内科学 | 1999篇 |
皮肤病学 | 104篇 |
神经病学 | 772篇 |
特种医学 | 460篇 |
外国民族医学 | 10篇 |
外科学 | 1487篇 |
综合类 | 2998篇 |
现状与发展 | 6篇 |
一般理论 | 1篇 |
预防医学 | 854篇 |
眼科学 | 327篇 |
药学 | 1568篇 |
15篇 | |
中国医学 | 872篇 |
肿瘤学 | 1115篇 |
出版年
2024年 | 34篇 |
2023年 | 202篇 |
2022年 | 474篇 |
2021年 | 777篇 |
2020年 | 602篇 |
2019年 | 467篇 |
2018年 | 459篇 |
2017年 | 432篇 |
2016年 | 454篇 |
2015年 | 656篇 |
2014年 | 826篇 |
2013年 | 734篇 |
2012年 | 1115篇 |
2011年 | 1207篇 |
2010年 | 848篇 |
2009年 | 664篇 |
2008年 | 800篇 |
2007年 | 799篇 |
2006年 | 798篇 |
2005年 | 765篇 |
2004年 | 487篇 |
2003年 | 450篇 |
2002年 | 396篇 |
2001年 | 383篇 |
2000年 | 316篇 |
1999年 | 333篇 |
1998年 | 190篇 |
1997年 | 202篇 |
1996年 | 160篇 |
1995年 | 134篇 |
1994年 | 104篇 |
1993年 | 59篇 |
1992年 | 80篇 |
1991年 | 74篇 |
1990年 | 72篇 |
1989年 | 61篇 |
1988年 | 40篇 |
1987年 | 37篇 |
1986年 | 42篇 |
1985年 | 20篇 |
1984年 | 11篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 2篇 |
1979年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
跳伞对空降兵脂质过氧化系统影响的初步研究 总被引:6,自引:2,他引:4
为探讨跳伞对空降兵脂质过氧化水平的影响和可能的生物学意义,测定了跳伞与否空降兵血浆中超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽硫转移酶的活性和丙二醛浓度,并与正常人群的值作了比较分析。结果显示,与正常人群相比,未跳伞空降兵血浆中超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽硫转移酶的活性和丙二醛浓度增高,但无显著性差异(P〉0.05);与未跳僮空军兵相比,跳伞后空降兵血浆三种酶活性略为增高,但无显 相似文献
83.
创面异体移植培养表皮细胞后存活的判定 总被引:1,自引:0,他引:1
应用4种方法判定烧伤创面异体移植的培养表皮细胞存活情况:1.聚合酶链反应(PCR)查Y染色体特异性DNA片段;2.PCR扩增PMCT118长度多态性基因位点;3.PCR/SSOHLA-DQa分型;4.DNA指纹。共检测16例病人的27个活检标本,均证实有供者的DNA,最长时间为移植后92天。初步实验结果表明:培养表皮异体移植后可存活较长时间。 相似文献
84.
心房颤动是临床常见的心律失常,已有研究证明其与严重不良心脑血管事件(心力衰竭、脑卒中和心肌梗死)有关,目前全球心房颤动的患病人数超过了3 300万,预计未来40年内其患病率将增加1倍以上。多年来,医学相关人员在探究心房颤动的病理生理机制及开创改进其治疗方法等方面付出了大量努力。目前心房颤动的治疗管理仍是临床医学上的一个难题,尽管心房颤动治疗的手术消融和导管消融技术已逐渐趋于成熟,但对于心房颤动最佳的治疗方式、消融能量的选择尚无统一定论。导管消融通常需要多次手术且成功率低,而手术消融术后不良事件发生率较高。近年来,鉴于心脏外科医生和电生理学家之间的密切合作,结合导管及微创手术消融诞生了一种治疗心房颤动的新型策略——混合消融模式。混合消融克服了导管消融和微创手术消融的缺点,减少了不良结局,在治疗持续性心房颤动,尤其是长期持续性心房颤动上取得了可观的成效。本文主要通过回顾心房颤动消融的研究进展,对比分析目前混合消融模式治疗心房颤动的现有研究成果,归纳总结这种新型心房颤动治疗策略的优势与挑战,以期为临床心房颤动的治疗提供更多选择。 相似文献
85.
陡脉冲电场对荷瘤BALB/c小鼠癌细胞杀伤效应的实验研究 总被引:1,自引:0,他引:1
观测陡脉冲电场对荷瘤BALB/c小鼠癌细胞的杀伤效应。处理后的癌细胞在光镜下表现为细胞核固缩、核碎裂、核溶解;在电镜下表现为异染色质增加,边集,呈团块样改变,细胞质肿胀,脂滴数量增加,质膜破裂;核固缩,碎裂,线粒体肿胀。实验揭示了陡脉冲电场能有效地杀伤癌细胞,明显抑制了荷瘤小鼠恶性肿瘤的生长、增殖,有着良好的应用前景。 相似文献
86.
In this paper, we investigate variance component models of both linkage analysis and high resolution linkage disequilibrium (LD) mapping for quantitative trait loci (QTL). The models are based on both family pedigree and population data. We consider likelihoods which utilize flanking marker information, and carry out an analysis of model building and parameter estimations. The likelihoods jointly include recombination fractions, LD coefficients, the average allele substitution effect and allele dominant effect as parameters. Hence, the model simultaneously takes care of the linkage, LD or association and the effects of the putative trait locus. The models clearly demonstrate that linkage analysis and LD mapping are complementary, not exclusive, methods for QTL mapping. By power calculations and comparisons, we show the advantages of the proposed method: (1) population data can provide information for LD mapping, and family pedigree data can provide information for both linkage analysis and LD mapping; (2) using family pedigree data and a sparse marker map, one may investigate the prior suggestive linkage between trait locus and markers to obtain low resolution of the trait loci, because linkage analysis can locate a broad candidate region; (3) with the prior knowledge of suggestive linkage from linkage analysis, both population and family pedigree data can be used simultaneously in high resolution LD mapping based on a dense marker map, since LD mapping can increase the resolution for candidate regions; (4) models of high resolution LD mappings using two flanking markers have higher power than that of models of using only one marker in the analysis; (5) excluding the dominant variance from the analysis when it does exist would lose power; (6) by performing linkage interval mappings, one may get higher power than by using only one marker in the analysis. 相似文献
87.
目的: 明确同型半胱氨酸(Hcy)对内皮细胞凋亡的影响以及叶酸的拮抗作用,阐明Bax和Bcl-2在同型半胱氨酸诱导内皮细胞凋亡及叶酸拮抗中的作用。方法: 用不同浓度的Hcy处理内皮细胞后,应用末端转移标记技术(TUNEL)以及Annexin V/PI染色加流式细胞术了解细胞凋亡状态,免疫组化方法检测Bax、Bcl-2的表达。结果: Hcy能促进细胞凋亡,叶酸具有拮抗作用。Hcy能促进细胞Bax、Bcl-2的表达,上调Bax/Bcl-2比值,叶酸能减少细胞表达Bax及Bcl-2,下调Bax/Bcl-2比值。结论: Bax、Bcl-2参与了Hcy诱导内皮细胞凋亡以及叶酸拮抗作用的过程。 相似文献
88.
以体外培养的猪主动脉内皮细胞(EC)为模型研究Ilopost对EC抗血栓功能的影响。用放免法、发色底物法测定培养液中6-酮-PGF1α(6-keto-PGF1α),血栓素B2(TXB2)含量,纤溶酶原激活物(PA),纤溶酶原激活物抑制物(PAI)活性及EC环磷酸腺苷(cAMP)含量。结果表明:Iloprost中以使EC生成6-keto-PGF1α,cAMP含量显著增多,TXB2含量显著减少,并使P 相似文献
89.
Krushkal J; Xiong M; Ferrell R; Sing CF; Turner ST; Boerwinkle E 《Human molecular genetics》1998,7(9):1379-1383
Elevated blood pressure is an important risk factor for renal-, cerebro-
and cardiovascular diseases. We used an efficient discordant sib-pair
ascertainment scheme to investigate the impact of the distal end of the
long arm of human chromosome 5 (chromosomal region 5q31.1-qter) containing
genes for the alpha1B and beta2 adrenergic receptors and the dopamine
receptor type 1A on variation of systolic blood pressure in young
Caucasians. We measured eight highly polymorphic markers spanning this
positional candidate gene-rich region in 427 individuals from 55
three-generation pedigrees containing 69 discordant sibling pairs, and
calculated multipoint identity by descent (MIBD) probabilities. The results
of genetic linkage and association tests indicate that the region between
markers D5S2093 and D5S462 is significantly linked to one or more
polymorphic genes influencing interindividual variation in systolic blood
pressure levels. Since the alpha1B adrenergic receptor and dopamine
receptor type 1A genes are located close to these markers, these data
suggest that genetic variation in one or both of these G protein-coupled
receptors, which participate in the control of vascular tone, plays an
important role in influencing interindividual variation in systolic blood
pressure levels.
相似文献
90.
Yuning Xiong Kathleen A Donovan Michael P Kline Michael K Gornet Laurie L Moon-Tasson Martha Q Lacy Angela Dispenzieri Morie A Gertz Philip R Greipp John A Lust 《Journal of interferon & cytokine research》2006,26(2):83-95
Interleukin-1beta (IL-1beta) is abnormally expressed by the plasma cells obtained from myeloma patients, and it is a potent inducer of the important myeloma growth factor, IL-6. We investigated whether levels of IL-1beta biologic activity might distinguish different groups of patients with smoldering multiple myeloma (SMM). We measured the ability of IL-6 production by bone marrow stromal cells to serve as a surrogate marker for IL-1beta biologic activity. Using this IL-1beta bioassay, we found that it is sensitive at < 1 pg/ml of recombinant IL-1beta and that IL-1beta biologic activity is detectable with either mature or pro-IL-1beta-transduced myeloma cell lines. Patients with active myeloma induced quantitatively higher levels of stromal cell IL-6 production when compared with those with monoclonal gammopathy of undetermined significance (MGUS). The bioassay distinguished two groups of SMM patients, those who were high producers, similar to patients with active MM, and those who were low producers, comparable to MGUS patients. IL-1 antagonists inhibited the paracrine IL-6 production by > or = 90% in the majority of patients with an elevated IL-6 level. Based on such studies, it may be possible to predict patients that will progress to active MM and to delay or prevent this progression with IL-1 antagonists. 相似文献