血清HE4、CA125、VEGF联合检测对卵巢癌诊断价值分析

目的 探讨血清人附睾蛋白4（HE4）、癌抗原-125（CA125）、血管表皮生长因子（VEGF）联合检测对卵巢癌诊断价值,为临床诊断提供一定的参考价值。方法 本研究病例来源于2013年5月~2015年5月期间笔者医院收治的107例卵巢疾病患者,按照术后病理结果将其分为卵巢癌组43例与良性病变组64例。选择同期于笔者医院体检中心体检的健康女性50例作为健康对照组。检测各组血清HE4、CA125、VEGF水平变化及联合检测敏感度和特异性。结果 卵巢癌组和卵巢良性病变组血清HE4水平明显高于健康对照组（P<0.05）;卵巢癌组血清HE4水平明显高于卵巢良性病变组（P<0.05）;卵巢癌组和卵巢良性病变组血清CA125水平明显高于健康对照组（P<0.05）;卵巢癌组血清CA125水平明显高于卵巢良性病变组（P<0.05）;卵巢癌组和卵巢良性病变组血清VEGF水平明显高于健康对照组（P<0.05）;卵巢癌组血清VEGF水平明显高于卵巢良性病变组（P<0.05）;血清HE4+CA125+VEGF联合检测敏感度和特异性明显高于单检测血清HE4、CA125、VEGF（P<0.05）。结论 血清HE4、CA125、VEGF在卵巢癌患者中呈高表达,3种肿瘤标志物联合检测具有着较高的敏感度和特异性,可作为诊断卵巢癌的重要手段,具有重要研究价值。     

A simple automated stimulator of mechanically induced arrhythmias in the isolated rat heart

Transient stretching of the ventricle can trigger arrhythmias and evoke ventricular fibrillation, especially when the stimulation occurs in the vulnerable period. To explore the sensitivity of small hearts we used a commercial pressure servo to study the kinetic relationship of left ventricular pressure to excitability and arrhythmias in the rat heart. Stimulation protocols were readily composed on the computer and programmed to vary the stimulus amplitude and timing relative to pacing. The pressure-induced premature ventricular excitations were similar to those observed in larger hearts, but the convenience of using small hearts allows the use of inexpensive transgenic animals to explore the molecular basis of transduction.     

支气管哮喘患者外周白细胞糖皮质激素的受体变化

用放射配基结合分析法测定了外源性及内源性哮喘患者外周白细胞糖皮质激素受体（ＧＣＲ）水平，同时测定其血浆皮质醇水平。结果发现哮喘患者ＧＣＲ水平较正常对照组明显降低（Ｐ＜０．０１），其降低程度与疾病初发年龄、病程密切相关，而哮喘患者血浆皮质醇水平与正常对照组无显著差异性。提示哮喘患者肾上腺皮质功能尚未受到明显损害，但其ＧＣＲ功能存在异常。关于哮喘患者ＧＣＲ功能异常原因，我们推测与哮喘患者遗传倾向有关，同时与环境因素反复刺激、疾病反复发作有关。     

GC-MS法分析蛇床子的脂溶性成分

〗摘 要 目的： 研究蛇床子的脂溶性成分。方法: 用索氏提取法提取蛇床子的脂溶性成分,甲酯化处理后,采用气相色谱 质谱联用技术首次对其脂溶性成分进行分析和鉴定。结果: 从蛇床子中鉴定了39个化合物,占蛇床子脂溶性成分的85.33%,蛇床子主要的脂溶性成分为植物醇(15.98%)、棕榈酸(12.73%)、9,12,15 十八碳三烯酸(11.02%)、油酸(6.33%)和9,12 十八碳二烯酸(4.77%)。结论:植物醇、棕榈酸、9,12,15 十八碳三烯酸和油酸是蛇床子的活性成分。     

Adsorption activity of coconut (Cocos nucifera L.) cake dietary fibers: effect of acidic treatment,cellulase hydrolysis,particle size and pH

The effects of acidic treatment, cellulase hydrolysis, particle size distribution and pH on the adsorption capacity of defatted coconut cake dietary fibers (DCCDF) were studied. The results demonstrated that cellulase hydrolysis could significantly improve the soluble dietary fiber content, water holding ability and adsorption ability of DCCDF on cholesterol, bile and nitrite ions. Acidic treatment enhanced the oil holding capacity and adsorption ability in cholesterol and nitrite ions. Moreover, the adsorption ability of DFs in cholesterol, nitrite and bile all increased with reduced particle size (250 to 167 μm), and DCCDF demonstrated a higher adsorption capacity at pH 2.0 than at pH 7.0. The change in adsorption capacity of DCCDF might be suitable for application in the food industry as a low-calorie and cholesterol lowering functional ingredient.

This paper provides valuable information on the effects of cellulase hydrolysis, acidic treatment, particle size distribution and pH on the adsorption activity of coconut cake dietary fibers.     

ASPP2表达对食管癌术后疗效评价意义的研究

目的:探讨ASPP2在食管癌术后患者中的表达水平及意义,证实ASPP2对食管癌术后疗效评价的可行性。探讨ASPP2在食管癌组织转变过程中的分子作用机制,ASPP2表达与食管癌患者术后疗效的关系。方法:采用免疫组化方法检测45例食管癌、20例食管良性瘤及20例正常食管组织中ASPP2、P53的表达水平。进一步分析ASPP2、P53的表达与食道癌临床病理特征的关系。采用Spearman等级相关的方法,对ASPP2、P53的表达水平进行相关性分析。结果:三组标本组织中ASPP2、P53的表达水平比较P<0.05,具有显著性差异。结论:食管癌组织中ASPP2表达显著下降,P53水平明显增高,ASPP2、P53的表达与食管癌的直径侵袭和淋巴结转移有关,ASPP2、P53的异常表达是食管癌发生和发展的重要因素,ASPP2是判断食管癌术后疗效的较好指标。     

Zein regulating apatite mineralization,degradability, in vitro cells responses and in vivo osteogenesis of 3D-printed scaffold of n-MS/ZN/PCL ternary composite

Bioactive and degradable scaffolds of nano magnesium silicate (n-MS)/zein (ZN)/poly(caprolactone) (PCL) ternary composites were prepared by 3D-printing method. The results showed that the 3D-printed scaffolds possessed controllable pore structure, and pore morphology, pore size, porosity and pore interconnectivity of the scaffolds can be efficiently adjusted. In addition, the apatite-mineralization ability of the scaffolds in simulated body fluids was obviously improved with the increase of ZN content, in which the scaffold with 20 w% ZN (C20) possessed excellent apatite-mineralization ability. Moreover, the degradability of the scaffolds was significantly enhanced with the increase of ZN content in the scaffolds. The degradation of ZN produced acidic products that could neutralize the alkaline products from the degradation of n-MS, which avoid the increase of pH value in degradable solution. Furthermore, the MC3T3-E1 cells responses (e.g. proliferation and differentiation, etc.) to the scaffolds were significantly promoted with the increase of ZN content. The in vivo osteogenesis of the scaffolds implanted the femur defects of rabbits was investigated by micro-CT and histological analysis. The results demonstrated that the new bone formation was significantly enhanced with the increase of ZN content, in which the C20 scaffold induced the highest new bone tissues, indicating excellent osteogenesis. The results suggested that the ZN in the ternary composite scaffolds played key roles in assisting bone regeneration in vivo.

Zein regulating apatite mineralization, degradability, cells responses and osteogenesis of 3D-printed scaffold of n-MS/ZN/PCL ternary composite.