大承气汤对全身性炎症反应干预作用的实验研究

[目的]探讨大承气汤对全身炎症反应综合征(SIRS)的干预作用的机理。[方法]选用酵母多糖A诱导的小鼠SIRS模型，给予大承气汤后，分别在6h，12h和24h等时点用鲎试剂微量法测定小鼠血清内毒素含量，用放射免疫法测定血清肿瘤坏死因子—α(TNF—α)和白细胞介素—6(IL—6)含量。[结果]SIRS状态下模型小鼠血清内毒素、TNF—α、IL—6均升高；与同时点模型组比较，大承气汤6h、12h时点可以明显抑制血清内毒素的升高(P＜0．05或P＜0．01)；大承气汤6h组TNF—α水平降低(与造模后6h组比，P＜0.05)；血清IL—6水平方面，大承气汤各组比正常组升高的幅度均较模型各组低，在24h时点大承气汤组比模型组显著降低(P＜0．05)。[结论]大承气汤在SIRS过程中可以有效地抑制内毒素的转移和TNF—α、IL—6等炎症反应性细胞因子的产生。     

抗Stat3多克隆抗体的制备和其在乳腺癌诊断中的应用

目的:制备兔抗信号转导与转录激活因子3(Stat3)抗体,分析其在乳腺癌诊断中的价值.方法:应用人工重组小鼠Stat3蛋白,免疫雄性家兔制备抗Stat3抗体;用免疫印迹法分析检测抗Stat3抗体的纯度和特异性;应用免疫组化染色法检测65例乳腺癌和30例正常乳腺组织中Stat3蛋白的表达.结果:用Stat3蛋白免疫家兔被免疫6 wk后,其血清中抗Stat3抗体效价＞1∶ 256,000(A450 nm=0.40);SDS-PAGE和免疫印迹分析均在分子质量(Mr)92 000处有明显的带;65例乳腺癌组织的细胞浆和细胞核中,Stat3蛋白表达量在不同的组织学分级之间有统计学差异(P＜0.05).结论:高效价和特异性强的抗Stat3抗体被成功制备. 该抗体可用于乳腺癌组织细胞Stat3蛋白表达的检测.     

大鼠胚胎小脑的发育学研究

用Ｎｉｓｌ染色法对大鼠胚胎１４、１６、１８及２０ｄ小脑进行形态学观察。结果表明：胚胎１６ｄ以前小脑由室管膜层、外套层及边缘层构成。胚胎１８ｄ开始出现典型的皮层结构。外颗粒层由较密集的深染细胞组成，数目随着胎龄的增加而增多。蒲氏层由较大淡染细胞组成，数目随着胎龄的增加而逐渐减少。颗粒层内细胞成份较复杂，细胞密度随胎龄增加呈下降趋势。     

橡胶防老剂D致突作用研究

防老剂 D 是以 N—苯基—β—萘胺为主要成份的一种橡胶工业污染物。亦是一种可疑的致突、致癌物或协同致突、致癌物。本文的研究结果发现防老剂 D 能显著地增加小鼠骨髓多染红细胞和大鼠胎鼠肝多染红细胞的微核发生率,说明防老剂 D 是一种致突物,具有胚胎毒性。     

LINC00240 knockdown inhibits nasopharyngeal carcinoma progress by targeting miR‐26a‐5p

ObjectiveThis study intended to explore the regulatory functions of LINC00240 on nasopharyngeal carcinoma (NPC).MethodsMiR‐26a‐5p inhibitor, mimic, and siLINC00240 were transfected into NPC cells. QRT‐PCR was employed to assess miR‐26a‐5p and LINC00240 expressions. The targeting relationship of LINC00240 and miR‐26a‐5p was analyzed through dual luciferase reporter and RNA immunoprecipitation assay. Cell counting kit‐8 assay, colony formation assay, flow cytometry assay, wound healing assay, Transwell assay and in vitro angiogenesis assay were adopted for the evaluation of the effects of LINC00240 or miR‐26a‐5p and LINC00240 on NPC cells regarding cell proliferation, apoptosis and cycle, migration, invasion, and angiogenesis. EZH2, cell cycle, and epithelial‐mesenchymal transition (EMT)‐related protein expression was tested through Western blot.ResultsLINC00240 had a high expression in NPC tissues and cell lines. Silenced LINC00240 significantly suppressed the 5‐8F and HK1 cell proliferation, invasion, migration, and angiogenesis, but raised cell apoptosis, and cells were blocked in G0/G1 phase. MiR‐26a‐5p was a target of LINC00240. MiR‐26a‐5p upregulation suppressed the NPC cell proliferation, migration, invasion, angiogenesis, N‐cadherin and EZH2 expression, while it elevated apoptosis and p21, p27 and E‐cadherin expressions, whereas miR‐26a‐5p downregulation performed conversely. LINC00240 knockdown partially offset the effects of miR‐26a‐5p downregulation on cell proliferation, migration, invasion, angiogenesis, apoptosis, and EZH2.ConclusionLINC00240 knockdown restrained cell proliferation, invasion, migration, and angiogenesis, while it advanced apoptosis via miR‐26a‐5p in NPC by EZH2 inhibition.     

男性生殖亚健康中医"以肾为本"

根据"男性生殖健康"概念以及目前对于亚健康诊断的诸多标准,首次提出"男性生殖亚健康"概念,从病因、症状(男性生殖细胞数量下降、性功能减退、男性更年期综合征、前列腺亚健康态)等方面作出分析,并从中医理论"肾藏精、主生殖"入手.阐述男性生殖亚健康的发生机制重在肾的阴阳失衡、精气不足,临床治疗应遵循"治未病"的思想,以防为主,防治结合,"以肾为本"干预男性生殖亚健康.     

Predictive model of postoperative pneumonia after neoadjuvant immunochemotherapy for esophageal cancer

BackgroundPostoperative pneumonia (PP) is the most common pulmonary complication of esophagectomy. It is of great importance to identify any high-risk factors and prevent pulmonary complications to improve the prognosis of patients with esophageal cancer undergoing esophagectomy. Thus, we established a predictive model of PP in patients with neoadjuvant immunochemotherapy for resectable esophageal squamous cell carcinoma (ESCC), and provide suggestions for the best strategy for the perioperative period of the patients.MethodWe retrospectively analyzed 78 patients who underwent esophagectomy for squamous cell carcinoma after neoadjuvant immunochemotherapy between September 2019 and August 2021.We used the “glmnet” language package in R to perform least absolute shrinkage and selection operator (LASSO) regression to screen the best predictors of PP, and nomograms predicting PP were constructed utilizing screened factors. The performance of nomograms was internally validated by calibration curves, concordance index (C-index), and the Brier score for overall performance.ResultsTwenty-six patients (33.3%) had postoperative pneumonia. After LASSO regression, the factors that were independently associated with PP were diffusing capacity of the lungs for carbon monoxide (DLCO) (P=0.0002), white blood cell (WBC) difference before vs. after neoadjuvant immunochemotherapy (P=0.0133). We constructed a prediction model, plotted the nomogram, and verified its accuracy. Its Brier score was 0.147, its calibration slope was 0.98, and its C-index was 0.85 (95% CI: 0.75–0.95). Internal validation demonstrated a good discrimination power that the actual probability corresponds closely with the predicted probability.ConclusionsOur prediction model can predict the possibility of PP in patients with neoadjuvant immunochemotherapy for resectable esophageal squamous cell carcinoma and may facilitate physicians’ efforts to reduce the incidence of postoperative pneumonia.