白细胞介素12对肝癌基因治疗的实验研究

目的 研究携带白细胞介素１２（ＩＬ－１２）基因逆转录病毒载体对体内肝癌生长抑制作用,探索基因治疗肝癌新途径。方法 构建携带ＩＬ－１２的逆转录病毒载体,转染逆转录病毒包装细胞系ＰＡ３１７,应用该包装细胞对实验性肝癌大鼠进行治疗,观察抗肿瘤作用,免疫功能变化。     

153例"摇头丸"滥用者社会学特征及相关因素分析

目的：分析“摇头丸”滥用者社会学特征及相关因素，为进行有效心理干预提供依据。方法：采用自行设计的《“摇头丸”滥用者心理社会学特征调查表》对北京市安康医院强制戒毒所2002年6月5日-2003年4月14日收治的153例“摇头丸”滥用者进行逐项登记，并对“摇头丸”服用方式、服用场所、对“摇头丸”认知情况以及经济状况进行比较。结果：“摇头丸”滥用者多以好奇为主，服用场所以迪厅、歌厅等娱乐场所为主、对“摇头丸”认知欠缺，经济状况尚好。结论：“摇头丸”滥用者虽然知识结构和经济状况尚好，但毒品知识欠缺，并以群体服用为主。     

自体微小颗粒骨复合碱性成纤维细胞生长因子修复节段性骨缺损的实验研究

目的 探讨自体微小颗粒骨复合碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)治疗骨缺损的效果，为其临床应用提供实验依据。方法 将36只新西兰白兔双侧桡骨中上段制成骨缺损模型，分别植入自体微小颗粒骨、bFGF和自体微小颗粒骨与bFGF的复合物，同时设空白对照组，分别手术后第2、4、8周进行大体观察，X检查及组织学检查。术后第8周进行骨密度测定，比较修复骨缺损的疗效。结果 经大体观察、组织学及X线检查发现，术后第8周自体微小颗粒骨复合bFGF能更有效地修复节段性骨缺损，对照组无骨愈合迹象。术后第8周骨密度测定证明自体微小颗粒骨复合bFGF组骨密度值明显高于对照组。结论 自体微小颗粒骨复合bFGF组成骨速度快，成骨量多，修复骨缺损的能力明显强于自体微小颗粒骨组及单纯bFGF组。     

A lipophilic free radical initiator, 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN) enhances caspase-dependent apoptosis induced by hyperthermia.

PURPOSE: A free radical initiator, 2,2'-azobis (2-amidinopropane) dehydrochloride (AAPH), was previously found to enhance apoptosis by hyperthermia. Here, but more lipophilic free radical initiator, 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN) was investigated for its effects as a possible heat sensitizer. MATERIALS AND METHODS: Human myelogenous monocytic leukaemia U937 cells were treated with hyperthermia combined with a various concentration of AMVN for investigating its ability to induce apoptosis and various parameters to identify the pathway. RESULTS: Combined treatment of hyperthermia and AMVN induced DNA fragmentation markedly, while hyperthermia or AMVN alone induced marginal DNA fragmentation. Fractions of cells showed low mitochondrial membrane potential and increased superoxide production after the combined treatment. Experiments using various caspase inhibitors and a fluorogenic monitor of caspase 3 activities indicated that caspase acts both up- and down-stream of mitochondria. CONCLUSIONS: AMVN is suggested to be a potential heat sensitizer effective at a lower concentration than AAPH. The possible mechanism is discussed.     

ATP-dependent para-aminohippurate transport by apical multidrug resistance protein MRP2

BACKGROUND: Para-aminohippurate (PAH), a widely used model substrate for organic anion transport in proximal tubule epithelia, was investigated as a substrate for the apical multidrug resistance protein MRP2 (symbol ABCC2). This ATP-dependent export pump for anionic conjugates and additional amphiphilic anions was cloned recently and localized to the apical membrane of proximal tubules in human and rat kidney. METHODS: Membrane vesicles from HEK-MRP2 cells containing recombinant human MRP2 and from control vector-transfected HEK-Co cells were incubated with various concentrations of [3H]PAH, and the net ATP-dependent transport into inside-out vesicles was determined. Comparative studies were performed with membrane vesicles containing recombinant human MRP1. RESULTS: Transport rates at 10 micromol/L PAH were 21.9 +/- 1.9 and 1.6 +/- 0.4 pmol x mg protein-1 x min-1 (means +/- SEM, N = 10) with membrane vesicles from HEK-MRP2 and HEK-Co cells, respectively. The Km value for PAH was 880 micromol/L. The high-affinity substrate leukotriene C4 and the inhibitor of MRP-mediated transport, MK571, inhibited MRP2-mediated transport of PAH (100 nmol/L) with IC50 values of 3.3 and 4.0 micromol/L, respectively. The nephrotoxic mycotoxin ochratoxin A inhibited MRP2-mediated PAH transport with an IC50 value of 58 micromol/L. Ochratoxin A was itself a substrate for MRP2. CONCLUSIONS: PAH is a good substrate for the ATP-dependent export pump MRP2. The localization and function of MRP2 indicate that this unidirectional transport protein contributes to the secretion of PAH and other amphiphilic anions into the lumen of kidney proximal tubules.     

鼻咽分泌物中β2微球蛋白测定的临床意义

To study its clinical significance, beta 2-microglobulin (beta 2-MG) in nasopharyngeal secretion was examined using radioimmunoassasy methods in 73 patients with nasopharyngeal carcinoma (NPC), 30 with chronic nasopharyngitis and 30 normal control subjects. In 31 NPC patients, the examination was repeated before and after radiotherapy. The average concentrations of beta 2-MG were 4.87 +/- 2.51 mg/L in NPC group, 1.05 +/- 0.64 mg/L in chronic nasopharygitis group, and 0.85 +/- 0.32 mg/L in the control. The beta 2-MG concentration was higher in the NPC patients than in both chronic pharyngitis patients and normal subjects (P < 0.01). The concentration was lowered down in NPC patients after radiotherapy, and remained high when the treatment failed to control the condition. It seems that beta 2-MG level in nasopharyngeal secretion is closely related to the prognosis and recurrence of nasopharyngeal carcinoma.     

对41例儿童旋毛虫病的临床观察

为了解儿童旋毛虫病的临床特征，对１９９２～１９９５年就诊的４１例儿童旋毛虫病进行分析。结果表明，患儿来自河南６个地区，主要因食猪肉馅饺子、烤羊肉串或食涮猪肉而感染。冬季为本病的高发季节。患儿平均年龄为８．８岁，男女之比为２．７１。长期发热和嗜酸性粒细胞增多为本病的主要表现，仅少数患儿有肌痛、胃肠道症状和皮疹。眼睑水肿仅见于发病早期。血清学检查对诊断本病有重要意义。预防本病的关键措施是加强肉检和改变不良的饮食习惯。     

慢性鼻窦炎的定义及发病因素浅析

长期以来对于慢性鼻窦炎的定义一直缺乏统～认识，这阻碍了人们对其病因、病理生理学的深入研究。近年来，随着鼻科学及相关基础学科的发展，人们对慢性鼻窦炎的定义展开了广泛的讨论，同时认识到慢性易窦炎是多种因素相互影响、相互作用的结果，临床上针对这些环节进行综合治疗有助于提高疗效、减少复发。1慢性耳塞炎的定义定义慢性鼻窦炎的关键在于区分急性和慢性鼻窦炎。现代观点认为区别急性与慢性疾病应根据病理学表现而非严格的时间考虑[1]，故一些学者将慢性鼻窦炎定义为粘膜存在明显的、持续的、不可逆的损伤、通过保守治疗难以解决…     

超选择动脉栓塞治疗血管搏动性耳鸣（附2例报告）

采用数字减影血管造影技术超选择性动脉栓塞治愈２例血管搏动怀耳鸣，并就发病原因、机理、治疗措施、临床特征进行讨论。