Aortenisthmusstenose mit offenem Ductus Botalli als Ursache einer schweren Herzinsuffizienz bei einer 36-j?hrigen Patientin –Fallbericht über eine erfolgreiche operative Behandlung Fallbericht über eine erfolgreiche operative Behandlung

Wir beschreiben den Krankheitsverlauf einer 36-j?hrigen Frau mit unbehandelter Aortenisthmusstenose und offenem Ductus Botalli, die aufgrund einer ausgepr?gten linksventrikul?ren Dysfunktion eine therapierefrakt?re Herzinsuffizienz entwickelte. Nach operativer Korrektur der Aortenisthmusstenose und Ductusresektion normalisierte sich die linksventrikul?re Pumpfunktion, sodass davon ausgegangen werden kann, dass auch schwere linksventrikul?re Dysfunktionen auf dem Boden langj?hriger Druck- und Volumenbelastung durch angeborene Herzfehler nach operativer Korrektur reversibel sein k?nnen.     

Maternal outcomes according to mode of delivery in women with severe preeclampsia: a cohort study

Objective: To determine the association between mode of delivery and maternal complications in patients with severe preeclampsia.

Methods: A prospective cohort study was conducted with 500 pregnant women with severe preeclampsia. The mode of delivery, vaginal or caesarean section, was considered the exposure, while the postpartum maternal complications and severe maternal morbidity were the outcomes. Logistic regression analysis was performed to determine the adjusted risk and 95% confidence intervals (95% CI) of maternal morbidity.

Results: Labour was spontaneous in 22.0% and induced in 28.2%, while 49.8% had an elective caesarean section. Ninety-five (67.4%) of the patients in whom labour was induced delivered vaginally. Total Caesarean rate was 68.2%. The risk of severe maternal morbidity was significantly greater in patients submitted to Caesarean section (54.0% versus 32.7%) irrespective of the presence of labour. Factors that remained associated with severe maternal morbidity following multivariate analysis were a diagnosis of HELLP syndrome after delivery (OR?=?3.73; 95% CI: 1.55–9.88) and having a caesarean (OR?=?1.91; 95% CI: 1.52–4.57).

Conclusions: Caesareans are often performed in patients with severe preeclampsia and are associated with significant postpartum maternal morbidity. Induction of labour should be considered a feasible option in these patients.     

Rubus idaeus extract suppresses migration and invasion of human oral cancer by inhibiting MMP‐2 through modulation of the Erk1/2 signaling pathway

Raspberries (Rubus idaeus L.) have been extensively studies worldwide because of their beneficial effects on health. Recently reports indicate that crude extracts of Rubus idaeus (RIE) have antioxidant and anticancer ability. The aim of this study was to evaluate the mechanism of its antimetastatic ability in oral cancer cells. In this study, SCC‐9 and SAS oral cancer cells were subjected to a treatment with RIE and then analyzed the effect of RIE on migration and invasion. The addition of RIE inhibited the migration and invasion ability of oral cancer cells. Real time PCR, western blot and zymography analysis demonstrated that mRNA, protein expression and enzyme activity of matrix metalloproteinases‐2 (MMP‐2) were down‐regulated by RIE. Moreover, the phosphorylation of Focal adhesion kinase (FAK), src, and extracellular signal‐regulated kinase (ERK) were inhibited after RIE treatment. In conclusion, these results demonstrated that RIE exerted an inhibitory effect of migration and invasion in oral cancer cells and alter metastasis by suppression of MMP‐2 expression through FAK/Scr/ERK signaling pathway. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1037–1046, 2017.     

The role S‐nitrosylation in manganese‐induced autophagy dysregulation in SH‐SY5Y cells

Overexposure to manganese (Mn) has been known to induce nitrosative stress. The dysregulation of autophagy has implicated in nitric oxide (NO) bioactivity alterations. However, the mechanism of Mn‐induced autophagic dysregulation is unclear. The protein of Bcl‐2 was considered as a key role that could participate to the autophagy signaling regulation. To further explore whether S‐nitrosylation of Bcl‐2 involved in Mn‐induced autophagy dysregulation, we treated human neuroblastoma (SH‐SY5Y) cells with Mn and pretreated cells with 1400 W, a selective iNOS inhibitor. After cells were treated with 400 μM Mn for 24 h, there were significant increases in production of NO, inducible NO synthase (iNOS) activity, the mRNA and protein expressions of iNOS. Interestingly, autophagy was activated after cells were treated with Mn for 0–12 h; while the degradation process of autophagy‐lysosome pathway was blocked after cells were treated with Mn for 24 h. Moreover, S‐nitrosylated JNK and Bcl‐2 also increased and phospho‐JNK and phospho‐Bcl‐2 reduced in Mn‐treated cells. Then, the affinity between Bcl‐2 and Beclin‐1 increased significantly in Mn‐treated cells. We used the 1400 W to neutralize Mn‐induced nitrosative stress. The results showed that S‐nitrosylated JNK and Bcl‐2 reduced while their phosphorylation were recovered to some extent. The findings revealed that NO‐mediated S‐nitrosylation of Bcl‐2 directly affected the interaction between Beclin‐1 and Bcl‐2 leading to autophagy inhibition.     

Corneal antinociceptive effect of (-)-α-bisabolol

Context: (-)-α-Bisabolol (BISA) is a sesquiterpene alcohol widely used as scent in cosmetic preparations, perfumes, shampoos, toilet soaps and other toiletries with potential for use in the pharmaceutical area.

Objective: To evaluate the corneal antinociceptive efficacy of BISA and to analyze the best solubilizing agent.

Materials and methods: Acute corneal nociception was induced by the local application of hypertonic saline (5 M NaCl; 20?μL) to the corneal surface of Swiss mice (n?=?8/group) 60?min after topical treatment with solutions or ointment containing BISA (50–200?mg/mL). The number of eye wipes performed with the ipsilateral forepaw was counted for a period of 30?s. Control groups (vehicles) were included.

Results: BISA (50, 100 or 200?mg/mL) solubilized with Tween 80 did not reduce the number of eye wipes. Animals treated with the ointment (BISA 50, 100 or 200?mg/mL; p?p?Discussion and conclusion: BISA possess corneal antinociceptive activity. Although the ointment presented antinociceptive effect, it is concluded that BISA when associated with propylene glycol has better potential for corneal nociceptive pain since it is more comfortable to use, leading to greater acceptance by patients.     

Depression and Its Correlates Among Brazilian Immigrants in Massachusetts,USA

The goal of this paper is to assess the frequency of depression symptoms among Brazilian immigrants living in Massachusetts, the second largest Brazilian immigrant population in the United States, and to identify correlates of depression. A convenience sample of Brazilian immigrants aged 18 or older residing in Massachusetts was used. Data were collected from December 2013 to March 2014, in the Consulate General of Brazil in Boston and in three religious events, using a structure questionnaire and the Center for Epidemiological Studies Depression Scale (CES-D). Depression symptoms were observed in 35.3% of the respondents, with equal distribution by sex. Correlates of depression were low income, being single, poor English proficiency, and poor self-perception of health. These results suggest a need for community outreach, sensitization, and counseling, in Portuguese and adapted to the culture of Brazilian immigrants.     

高血压病及糖尿病患者肾血流的彩色多普勒速度能量图评价及其影响因素分析

目的：本研究旨在观察高血压病（ＥＨ）及糖尿病（ＤＭ）患者肾动脉血流的彩色多普勒速度能量图（ＣＣＤ）变化并探讨其影响因素。方法：以正常对照组及ＥＨ组,ＤＭ组为研究对象,每组各６０例,均为男３０例,女３０例,对照组年龄３７～７５岁,ＥＨ组４０～７７岁,ＭＤ组４１～７３组,采用ＡＣＵＳＯＮ公司生产的ＡＳＰＥＮ彩色多普勒超声显像仪,探头频率４～２ＭＨＺ及３．５ＭＨＺ,应用ＣＣＤ测量肾动脉主干最大血流速度（