Coculture of vascular endothelial cells and adipose-derived stem cells as a source for bone engineering

The interaction between vascular endothelial cells (VECs) and osteoblasts (OBs) is the focus of this recent research. Vascular endothelial cells secrete bone morphogenetic protein, which promotes OB differentiation and stimulates OBs and their precursor cells to secrete vascular endothelial growth factor. Vascular endothelial growth factor is important in angiogenesis and angiopoiesis. Cloning studies have shown that adipose-derived stem cells (ADSCs) have the potential to differentiate into fat, bone, cartilage, and skeletal and smooth muscle cells, among others. Adipose-derived stem cells can express multiple growth factors, including vascular endothelial growth factor and hepatocyte growth factor. Our study examined the influence of coculturing VECs and ADSCs on osteogenic differentiation. Cord blood-derived VECs and ADSCs were isolated from rats and characterized with immunofluorescence staining and morphological observation. Coculture of third-generation ADSCs and VECs was induced for 6 weeks. Cell growth was analyzed using a modified MTT assay. Alkaline phosphatase (ALP) and osteocalcin (OC) was analyzed using immunofluorescence staining. When ADSCs and VECs were cocultured, the absorbance of cells gradually increased, reaching a peak on day 12. The highest absorbance was seen in a coculture system with a ratio of ADSCs and VECs of 1:1. The secretion of ALP and OC gradually increased in these cells and was significantly higher than controls (P < 0.01). Coculturing of ADSCs and VECs at a 1:1 ratio gave the highest secretion of ALP and OC at every time point, and was significantly higher than other groups (P < 0.01). Our results indicated that ADSCs can be induced to osteogenic differentiation by VECs in vitro, suggesting a coculture system of VECs and ADSC as a novel source of cells for bone engineering.     

强脉冲光治疗面部痤疮后红斑及炎性色素沉着236例疗效观察

目的:观察强脉冲光(I PL)治疗面部痤疮后红斑及炎性色素沉着的临床疗效。方法:采用皇后光子嫩肤仪对有面部痤疮红斑及炎性色素沉着的患者进行治疗,波长560～1200nm,能量密度16～28J/cm2,治疗3～5次为1个疗程,每次治疗间隔时间为4周,1个疗程后观察疗效。结果:236例患者中褐黑色炎性色素沉着51例,红斑137例,红斑加色素沉着48例,其中111例痊愈,显效101例,有效24例,总有效率达100%。结论:I PL治疗痤疮后红斑及炎性色素沉着无创、有效,可临床推广应用。     

关节镜引导下治疗涉及关节面的股骨髁部骨折

目的探讨关节镜引导下微创治疗股骨髁部骨折的临床效果及手术适应证。方法对11例股骨髁部骨折患者采用关节镜引导下复位空心螺钉固定,与之前相同类型骨折采用切开复位内固定的14例患者组进行比较,评估围手术期出血量、手术时间、围手术期并发症及术后膝关节功能。结果全部25例患者均获得随访10～27个月,平均17个月,两组均未发生伤口感染、骨不愈合并发症;关节镜组手术时间相对较长,出血量较少,术后功能恢复明显优于切开组。结论关节镜引导下复位空心螺钉固定股骨髁部骨折具有微创、功能恢复良好等优点,并可对膝内合并损伤进行诊治,对比切开复位具有一定优势。     

含Hp特异性抗体羊奶2种检测方法的比较及初步临床效果

［摘要］ 目的比较含抗Hp特异性抗体羊奶的2种检测方法,并初步观察抗体羊奶的临床效果。 方法用国际标准菌株ATCC26695及ATCC26695、SS1混合菌液制备2种含抗Hp特异性抗体的羊奶,分别用双相免疫扩散试验及酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA) 法检测羊奶中抗Hp特异性抗体含量,并比较二者检测效果;30名受试者按照随机抽样法分为试验组和对照组,试验组每天饮用抗体羊奶,对照组饮用普通羊奶,连续饮用30 d,用14C尿素呼气试验(14C urea breath test,14C-UBT) 评估效果。 结果2种方法检测结果均证实了抗体羊奶中抗Hp特异性抗体的存在,ELISA结果显示,2种抗体羊奶中抗Hp特异性抗体含量高于对照孔,差异有统计学意义(P＜0.05),且随着稀释倍数增大,抗体含量逐渐降低;2种抗体羊奶间抗体含量差异无统计学意义(P＞0.05)。初步临床试验证明试验组经服用抗体羊奶后有效率为66.6%,与服用前比较差异有统计学意义(P＜0.05)。 结论2种检测方法均可较好地反映抗体羊奶中抗Hp特异性抗体存在,ELISA法简便、快速,能够更为精确地定量检测抗体含量,所获得的结果更为可靠。临床试验表明抗体羊奶能够有效降低患者14C-UBT数值,具有良好的临床应用前景。     

一起由两种罕见血清型副溶血性弧菌引起食物中毒事件分析

目的 分析一起由两种罕见血清型混合感染引起的副溶血性弧菌食物中毒病原学的检测结果,为食物中毒病原学检查途径提供依据。方法 对一起在龙门县人民医院就诊食物中毒事件的9例患者采集其肛拭子样本,参照《食品微生物学检验副溶血性弧菌检验》( GB 4789.7—2013) 等标准进行病原学分析鉴定。结果 本次事件出现临床症状者9例,其中≤20岁2例,20~<40岁1例,40~<60岁4例,≥60岁2例;9份肛拭子样本检出8株副溶血性弧菌,血清分型O2:K28构成比占25.00%(2/8);O8:K21构成比占75.00%(6/8);经PFGE分子分型,2株O2∶K28血清型(VP18171、VP18175)相似度为66.7%;3株O8∶K21血清型(VP18172、VP18173、VP18176)相似度为100.0%,与另外3株O8∶K21血清型(VP18177、VP18174、VP18170)相似度为88.4%~95.2%;药敏试验结果显示,除有1株菌株对氨苄西林、头孢唑林二重耐药、1株菌株对氨苄西林耐药、3株菌株对头孢唑林耐药外,其它菌株对所有测试药物均敏感,无多重耐药现象。结论 此次食物中毒是由O2:K28和O8:K21这两种罕见血清型的副溶血性弧菌混合感染引起的。     

真核起始因子4E对小鼠动脉粥样硬化发生、发展的炎症调节作用*

目的 探讨真核起始因子4E（eIF4E）在小鼠动脉粥样硬化发生、发展过程中的炎症调节作用。方法 分别用200?ng/μl脂多糖（LPS）和20?ng/μl白细胞介素-4（IL-4）诱导RAW264.7细胞12?h后提取mRNA,24?h后提取蛋白,采用实时荧光定量聚合酶链反应（qRT-PCR）和Western blotting检测eIF4E mRNA和蛋白在M1型和M2型巨噬细胞中的表达变化。复制ApoE-/-动脉粥样硬化小鼠模型,检测小鼠腹主动脉eIF4E mRNA和蛋白表达变化;组织免疫荧光检测eIF4E在主动脉根部中分别与M1型和M2型巨噬细胞中的共表达。结果 M1型巨噬细胞eIF4E mRNA和蛋白表达高于M2型巨噬细胞（P?<0.05）。在ApoE-/- 小鼠动脉粥样硬化模型中,ApoE-/-小鼠腹主动脉eIF4E mRNA和蛋白表达高于C57BL/6J小鼠（P?<0.05）;eIF4E与CD86的共定位表达高于eIF4E与CD206的共定位表达。结论 eIF4E在动脉粥样硬化的发展过程中主要在M1型巨噬细胞中起作用,进而对动脉粥样硬化产生影响。     

DC靶向适配体修饰的载铜绿假单胞菌DNA疫苗递送系统的构建及体外评价

构建了一种DC靶向适配体修饰的铜绿假单胞菌(Pseudomonas aeruginosa,PA)DNA疫苗递送系统。采用乙醇注入法制备阳离子脂质体,静电吸附法制备载pVAX1-OprF-VP22的阳离子脂质体(Lip-pOprF-VP22),探讨不同DOTAP/pDNA质量比的Lip-pOprF-VP22对pVAX1-OprF-VP22的包封效果、对DC2.4的细胞毒性及转染率,筛选最佳质量比的Lip-pOprF-VP22测定其粒径及Zeta电位;后插法制备DC靶向适配体修饰的载pVAX1-OprF-VP22的阳离子脂质体(Apt-Lip-pOprF-VP22),检测其转染DC2.4后OprF蛋白的表达量及对小鼠骨髓来源树突状细胞(bone marrow-derived dendritic cells,BMDCs)成熟的影响。结果表明,Lip-pOprF-VP22随着DOTAP/pDNA质量比增加包封率逐渐增加,当质量比为5∶1时即能很好的包封pVAX1-OprF-VP22;当Lip-pOprF-VP22作用于DC2.4 24 h或48 h后,不同质量比的Lip-pOprF-VP22对DC2.4的存活率均在80%以上;当DOTAP/pDNA质量比由2∶1增加到10∶1,转染率表现为先增加、后降低的趋势,其中DOTAP/pDNA质量比为4∶1、5∶1时转染率相对较高;当DOTAP/pDNA质量比为5∶1时,Lip-pOprF-VP22粒径为(171.67±1.27)nm,Zeta电位为(11.30±0.57)mV;Apt-Lip-pOprF-VP22转染DC2.4后可表达更多OprF蛋白且可明显促进BMDCs的成熟。     

临床药学领域专业学位研究生培养模式探索

本研究采用文献调研结合实践总结等方法,分析临床药学领域开展专业学位研究生培养的必要性。对现阶段专业课程设置、导师队伍、实践教学等方面存在的问题进行探讨;提出医科院校应发挥临床资源优势和特色,借鉴临床药师培训基地人才培养经验,优化课程体系,遴选导师队伍,重视实践教学等建议;以提高临床药学领域专业学位研究生教育质量,培养高水平临床药师,推动我国临床药学事业发展。     

肾3号方对IgA肾病大鼠的作用机制探讨

目的:观察肾3号方对IgA肾病(IgAN)大鼠的作用机制.方法:以牛血清球蛋白(bovine gamma globulin,BGG)免疫Lewis大鼠建立IgA 肾病大鼠模型.设正常对照组、IgAN组、IgAN 肾3号方治疗组.IgAN 肾3号方治疗组于第4周末～第8周末每日灌服肾3号方水提取液.蛋白质印迹法(Western blot)、免疫组化检测转化生长因子(TGF-β1)表达,免疫组化测IgA肾内沉积,进行尿红细胞、肾组织学检测.结果:与IgAN组比较,IgAN 肾3号方治疗组8周末无明显血尿(P<0.01),肾小球系膜增生程度减轻,免疫组化IgA在肾小球沉积明显减轻(P<0.01),免疫组化TGF-β1 肾小球阳性显著减轻(P<0.05),Western blot TGF-β1明显减少(P<0.05).结论:肾3号方能减轻IgAN鼠血尿,减轻肾组织病理损害,减轻肾小球IgA沉积和肾TGF-β1的表达和产生.     

The associations between diode laser (810 nm) therapy and chronic wound healing and pain relief: Light into the chronic wound patient's life

Chronic wounds have become one of the major issues in medicine today, the treatments for which include dressing changes, negative pressure wound therapy, hyperbaric oxygen, light irradiation, surgery and so forth. Nevertheless, the application of diode lasers in chronic wounds has rarely been reported. This retrospective cohort study aimed to evaluate the therapeutic effect of diode laser (810 nm) irradiation on chronic wounds. Eighty-nine patients were enrolled in the study. The control group (41 patients) received traditional dressing change therapy, while the diode laser treatment group (48 patients) were patients received additional treatment with diode laser (810 nm) irradiation for 10 min at each dressing change. Wound healing time was compared between two groups, while the pain relief index was creatively introduced to evaluate the effect of relieving wound pain, which was calculated by the difference in pain scores between the first and last dressing changes divided by the number of treatment days. The wound healing time of the diode laser treatment group was 22.71 ± 8.99 days, which was significantly shorter than that of the control group (37.44 ± 23.42 days). The pain relief index of the diode laser treatment group was 0.081 ± 0.055, which was significantly increased compared with that of the control group (0.057 ± 0.033). Our findings suggest that diode laser irradiation has the potential to promote healing in chronic wounds and relieve wound pain.