PRK治疗RK后残余近视散光二年疗效分析

目的： 评价准分子激光屈光性角膜切削术治疗放射状角膜切开术后残余近视散光的预测性、稳定性和安全性。方法： 对５２ 眼 Ｒ Ｋ 术后残余近视散光患者行再次 Ｐ Ｒ Ｋ 治疗, 并连续随访两年以上。术前残余球镜屈光度－ ３７５±１３２ Ｄ,残余柱镜屈光度－ ２３６±１２５ Ｄ, 最佳矫正视力均≥０８。结果： 术后 ６ 个月球镜屈光度－ ０３２±０２４ Ｄ, 柱镜屈光度－ ０７８±０５２ Ｄ, 裸眼视力≥１０ 者４６ 眼 （８８４６％ ）, 术后 ２４ 个月球镜屈光度－ ０３７±０４２ Ｄ, 柱镜屈光度－ ０５６±０３７ Ｄ, 裸眼视力≥１０ 者 ４２ 眼 （８０７６％ ）。最佳矫正视力下降 １ 行以上者 ３ 眼 （５７６％ ）, 实际球镜矫正度与预期矫正度相差１０ Ｄ 以内 ５０ 眼 （９０１５％ ）, 柱镜相差 １０ Ｄ以内５１ 眼 （９８０７％ ）。结论： Ｐ Ｒ Ｋ 治疗 Ｒ Ｋ 术后残余近视散光的两年随访结果表明, 该方法预测性强、稳定性好和安全性高, 特别在矫正残余散光方面, 其结果令人满意。     

Molecular characterization of a new excitatory insect neurotoxin with an analgesic effect on mice from the scorpion Buthus martensi Karsch.

Besides the neurotoxins active on mammals, a new excitatory insect selective toxin with a mice analgesic activity was found and purified from the venom of the scorpion Buthus martensi Karsch (BmK) (Ji, Y.H., Mansuelle, P., Terakawa, S., Kopeyan, C., Yanaihara, N., Hsu, K., Rochat, H., 1996. Toxicon 34, 987; Luo, M.J., Xiong, Y.M., Wang, M., Wang, D.C., Chi, C.W., 1997. Toxicon 35, 723.). This peptide (designated as BmK IT-AP) is composed of 72 amino acid residues. Its primary structure was determined by automated Edman degradation of the N-terminal part of the reduced and S-carboxamidemethylated protein and its lysylendopeptidase degraded fragments. Based on the determined sequence, the gene specific primers were designed and synthesized for 3' and 5' RACE (rapid amplification of cDNA ends). Their partial cDNA fragments obtained by 3' and 5' RACEwere cloned and sequenced and the full length cDNA sequence of BmK IT-AP was then completed by overlapping their two partial cDNA sequences. It encodes a precursor of 90 amino acid residues: a signal peptide of 18 residues and a mature peptide of 72 residues which are consistent with the determined protein sequence of BmK IT-AP. The genomic DNA of the peptide was also amplified by PCR from the scorpion genomic DNA and sequenced, which is a first report on the genomic structure of a scorpion toxin specific for insects. Its sequence revealed an intron of 590 bp inserted in the end part of the signal peptide. The peptide caused a fast excitatory contraction paralysis on house fly larvae. Furthermore, the peptide also showed an obvious analgesic effect on mice, as assayed by using a twisting test model. This effect of BmK IT-AP well characterized at molecular level is first reported among the known scorpion insect neurotoxins.     

银杏提取物对培养的脑微血管内皮细胞与单核细胞和中性粒细胞 …

AIM: To study the action of Ginkgo biloba extract (GbE) on tumor necrosis factor (TNF-alpha)-induced adhesion of monocytes (Mon) and neutrophils (Neu) to cultured cerebral microvascular endothelial cells. METHODS: TNF-alpha-induced endothelial adhesivity toward Mon and Neu was studied using bovine cerebral microvascular endothelial cells (BCMEC) in vitro. The number of Mon and Neu adhering to the BCMEC monolayers was determined by flow cytometry. RESULTS: Pretreatment of BCMEC with TNF-alpha increased Mon and Neu adhesion to BCMEC from 12.5% +/- 0.2% to 31.3% +/- 0.5% and from 13.8% +/- 0.4% to 32.1% +/- 0.5%, respectively. GbE (1-100 mg.L-1) inhibited the effect of TNF-alpha in a concentration-dependent manner. E-selectin mAb (1 mg.L-1) blocked Mon and Neu adhesion to BCMEC induced by TNF-alpha. CONCLUSION: The inhibition of GbE on Mon and Neu adhesion to BCMEC was mediated through the suppression of E-selection expression.     

左旋四氢巴马汀对单个豚鼠心室肌细胞钙电流的阻滞作用

AIM: To study the effect of l-tetrahydropalmatine (l-THP) on L-type calcium channel. METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2+ current in single cardiac myocyte. RESULTS: 1) l-THP 1, 10, and 100 micromol.L-1 reduced ICa-max from (999 +/- 93) pA to (700 +/- 111) pA, (582 +/- 66) pA, and (420 +/- 112) pA (n = 6, P < 0.01), respectively. 2) l-THP reduced the voltage at half-maximal inactivation (V1/2) of L-Ca2+ channel to more negative potentials by 9 mV (n = 5, P < 0.05). 3) l-THP caused both tonic and use-dependent reduction of Ca2+ current. Tonic block of l-THP on Ca2+ current was 46% +/- 8% (n = 6, P < 0.01). The degree of use dependent blocking was 13.5% +/- 2.4% (n = 6, P < 0.05) at 1 Hz, the degree increased to 44% +/- 5% (n = 6, P < 0.01) at 3 Hz. 4) l-THP delayed half-recovery time of Ca2+ channel recovery from inactivity from (94 +/- 39) ms to (170 +/- 42) ms(n = 6, P < 0.01). CONCLUSION: l-THP has a moderate inhibitory effect on L-Ca2+ current.     

对外科手术中意外低体温影响因素的观察研究

对３０例神经外科择期手术体温正常患者术中影响体温变化的因素进行观察。在国麻醉期持续监测体温、室温及相对湿度,记录液体输注量及尿量。结果显示,当室温≤２４℃、手术时间＞ ５小时、输注室温下人液量≥（６５１±１２１）ｍｌ／ｈ时,可导致意外低体温。所以,麻醉中体温监测是必不可少的。     

若干肿瘤标志物对原发性肝癌诊断的临床评价

目的综合评价血清甲胎蛋白（ＡＦＰ）、α－Ｌ－岩藻糖苷酶（ＡＦｕ）、γ－谷氨酰转肽酶（ＧＧＴ）、唾液酸（ＳＡ）和癌胚抗原（ＣＥＡ）对原发性肝癌（ＰＨＣ）的诊断价值以及联合检测的临床意义。方法对６７例ＰＨＣ、８例肝转移癌、８９例良性肝病及１３１例非肝病者,联合检测ＡＦＰ、ＡＦｕ、ＧＧＴ、ＳＡ和ＣＥＡ。结果５项指标对ＰＨＣ诊断的敏感性分别是６５．４％、７０．８％、３４．８％、６０．２％和３６．４％;特异性分别为９２．６％、９８．１％、８７．１％、９４．５％和７７．８％;ＡＦＰ、ＡＦｕ、ＧＧＴ和ＳＡ联合检测对ＰＨＣ诊断的敏感性是８１．４％,特异性为９１．６％。结论５种肿瘤标记物中,ＡＦｕ和ＡＦＰ对ＰＨＣ诊断意义较大;ＡＦｕ、ＡＦＰ、ＧＧＴ和ＳＡ联合检测可提高对ＰＨＣ诊断的敏感性和特异性。     

急性心肌梗死患者Q-T离散度的临床分析

目的 探讨急性心肌梗死患者与健康人QT离散度的关系。方法 观察61例急性心肌梗死患者QT离散度的变化并与58例健康人QT离散度比较，急性心肌梗死组15例发生室颤患者QT离散度的变化并与未发生室颤患者QT离散度比较。结果 急性心梗塞组QTdQTcd明显大于正常对照组，急性心肌梗死组发生室颤患者QTd及QTcd明显大于无室颤度者。结论 推测QTd作为无创性预测心脏事件方法有一定的临床意义。     

口服鸡Ⅱ型胶原治疗大鼠的佐剂型关节炎

目的 研究口服鸡Ⅱ型胶原对大鼠佐剂型关节炎的治疗作用。方法 建立大鼠佐剂型关节炎动物模型，通过胃管给药方式分别给予高、中、低剂量的鸡Ⅱ型胶原，对大鼠四肢关节进行评分，观察大鼠关节病变的发生和严重程度，采用免疫荧光技术，用流式细胞仪检测大鼠外周血T细胞亚群的变化，并且用大鼠rINFn检测试剂盒(固相夹心ELISA法)检测大鼠外周血TNFα水平的变化。结果 建立了大鼠佐剂型关节炎实验动物模型，口服高、中、低剂量的鸡Ⅱ型胶原均可减轻关节病变的发生，其中高剂量治疗组的作用最显著，而且大鼠外周血CD3 T细胞、CD4 T细胞和CD8 T细胞水平与关节炎模型组比较均有显著性差异，外周血TNFα水平也较模型组显著降低。结论 口服鸡Ⅱ型胶原可有效减轻实验性佐剂型关节炎大鼠的关节病变，其机理与口服耐受有关。     

醒脑酊质量控制方法的研究

目的 :建立醒脑酊的质量控制方法。方法 :采用薄层色谱法对醒脑酊中的薄荷脑、丁香、冰片进行定性鉴别 ;并采用气相色谱法对该药中的薄荷脑进行含量测定。气相色谱条件 :以萘为内标物 ,填充玻璃柱 (2.1mm× 3.2m ,Shimadzu 7 G ,) ,载气N₂ ,流速 50mL·min^-1 ,柱温 145℃ ;进样口及检测器温度 180℃ ,不分流方式进样 ,进样量3μL。 结果 :薄层色谱图斑点清晰 ,阴性对照无干扰 ;气相色谱法精密度、重现性良好 ,平均加样回收率为 98.4% ,RSD=0.9%。结论 :本方法快速简便 ,结果准确 ,可用于控制醒脑酊的质量。     

地肤子及其混淆品的扫描电镜与紫外法鉴别研究

分别采用扫描电镜和紫外光谱法对地肤子、藜、小藜和扫帚菜四种药材进行了比较鉴别。结果表明：扫描电镜下四种药材的胞果、毛茸、气孔和种子形态特征有较为明显的区别；紫外光谱扫描结果表明地肤子和扫帚菜无明显区别，但与藜和小藜之间有显著区别。