Long-term results of radio-surgical treatment of operable carcinoma of the cervix uteri

Four hundred and fifteen women, treated in our institution from 1961 to 1987 for operable carcinoma of the cervix stage I or II, had a radical hysterectomy with pelvic node dissection. Radiotherapy was associated with this surgery in most cases: 90% of the patients received endocavitary radiation, and 30% external radiotherapy, either pre- or post-operatively, in the case of bad prognostic factors. Median follow up was 7 years; overall survival was 77% at 10 years; the rate of local failure is low (36 cases/415 = 8.7%). Multifactorial analysis, using the Cox model, assessed the following independent prognostic factors, significant for the risk of local recurrence (size of the tumor, N +/- , FIGO stage) and for the risk of metastases and death (parametrium invasion, stage, tumor size). Post-operative mortality was 1.2%. Early surgical iatrogeny was easily treated with no long-term sequela, whereas severe complications, due to the association with pelvic irradiation, occurred in 24 out of 124 patients, leading to 7 "illegitimate" deaths.     

咪唑安定、异丙酚对体外循环期酸碱平衡的影响

目的 观察异丙酚和咪唑安定在体外循环麻醉中对术前和术中的酸碱度的影响.方法 40例先天性心脏病患者随机分为两组(异丙酚组、咪唑安定组),监测手术前、主动脉阻断10 min和复温至31℃时血气指标等的动态变化.结果与手术前比,主动脉阻断10 min和复温后,血液pH、PaCO2、HCO3-、BEB两组均有明显变化(P＜0.05),但两组间差异无显著性(P＞0.05).结论异丙酚和咪唑安定在体外循环麻醉中其对酸碱度无明显的影响.     

骨骼肌肌细胞增殖及分化过程中凋亡现象

目的 对骨骼肌分化中的凋亡现象进行初步研究,方法 本实验采用体外培养的C2C12肌母细胞分化模型,用流式细胞仪检测肌细胞分化过程中细胞周期的变化,提取细胞基因组DNA进行电泳分析,用原位末端标记法进行了凋亡细胞染色,电子显微镜观察凋亡小体的形成。结果 Ｃ２Ｃ１２细胞在肌分化诱导２４h和４８h,检测出凋亡现象;而对照组、肌分化诱导７２h组均未检出凋亡现象。结论 在肌肉分化、发育的过程中,某些细胞会按自身程序主动死亡,以凋亡细胞的形式排除。而且具有明显的时间性,终末分化的肌管不易出现凋亡。     

99mTc-DTPA气溶胶显像测定呼吸道粘液纤毛清除功能

为进一步研究呼吸道疾病时的粘液纤毛清除功能,采用99mTc-DTPA气溶胶吸入显像,通过电影显示定性和定量指标分析.对18 例健康受试者和患者进行了显像研究,得到气道清除率和粘液纤毛清除率的正常值,并观察了32例慢性阻塞性肺病(COPD)患者气管-支气管纤毛清除功能的改变.结果显示,健康受试者与COPD患者粘液纤毛清除率(mm/min)分别为3.89±0.92和1.32±0.59,两者比较有显著性差异(P＜0.01),COPD患者不同时间气道清除率和粘液纤毛清除率均明显低于健康受试者(P＜0.01).本研究方法简便客观,具有定性观察和定量分析的优点,对于呼吸系统其它疾病(如支气管扩张、原发性纤毛无运动)的研究及药物疗效的评价具有重要的临床实用价值.     

三个半规管阻塞动物模型的建立

目的 旨在建立豚鼠单侧三个半规管阻塞的动物模型。方法 利用２０只豚鼠行单侧三个半规管阻塞,观察手术前后眼震电图、听性脑干反应（ａｕｄｉｔｏｒｙ ｂｒａｉｎｓｔｅｍ ｒｅｓｐｏｎｓｅｓ,ＡＢＲ）、畸变产物耳声发射（ｄｉｓｔｏｒｔｉｏｎ ｐｒｏｄｕｃｔ ｏｔｏａｃｏｕｓｔｉｃ ｅｍｉｓｓｉｏｎ,ＤＰＯＡＥ）及形态学的变化,非手术耳作对照。结果 豚鼠术后１ｄ出现自发性眼震,正弦摆动刺激单侧眼震反应消失,     

甲状腺切除病例喉返神经损伤的分析

目的：研究甲状腺切除术的主要并发症－－喉返神经（ＲＬＮ）麻痹的相关因素。方法：回顾性研究１５６３例甲状腺手术患者的临床资料,重点分析ＲＬＮ损伤与术式、ＲＬＮ在术中是否被分离保护以及与甲状腺疾病的病理关系。结果：ＲＬＮ损伤率是７．８％,与组织病理恶性程度明显相关（Ｐ〈０．０１）,但术中如明确找到ＲＬＮ并加以保护,术后则无ＲＬＮ永外性损害,暂时麻痹仅１．６％。结论：术中对ＲＬＮ的保护应该强调避免医源性     

侧颅底肿瘤手术入路(附44例报告)

目的:探讨切除侧颅底肿瘤的最佳入路.方法:44例患者中,采用下颌外旋入路17例,耳后C形切口入路16例,上颌外旋入路7例,颞额入路4例.结果:25例良性肿瘤术后无复发;19例恶性肿瘤中,术后生存不足1年2例,1年以上4例,2年以上7例,3年以上6例.结论:下颌外旋入路适用于切除咽旁间隙、颞下窝、鼻咽部、口咽部、蝶骨、斜坡及上颈椎肿瘤;耳后C形切口入路适用于切除颞骨肿瘤.     

Screening and identification of 3-aryl-quinolin-2-one derivatives as antiviral agents against influenza A

Quinolin-2-one represents an important and valuable chemical motif that possesses a wide variety of biological activities; however, the anti-influenza activities of quinolin-2-one-containing compounds were rarely reported. Herein, we describe the screening and identification of 3-aryl-quinolin-2-one derivatives as a novel class of antiviral agents. The 3-aryl-quinolinone derivatives were synthesized via an efficient copper-catalyzed reaction cascade that we previously developed. Using this synthetic method, preliminary structure–activity relationships of this scaffold against the influenza A virus infection were systematically explored. The most potent compound 34 displayed IC₅₀ values of 2.14 and 4.88 μM against the replication of H3N2 (A/HK/8/68) and H1N1 (A/WSN/33) strains, respectively, without apparent cytotoxicity on MDCK cells. We further demonstrated that 27 and 34 potently inhibited the plaque formation of the IAV, rendering this scaffold attractive for pursuing novel anti-influenza agents.     

原发性高血压肝阳上亢证,肝肾阴虚证患者动态血压分析

对６２例原发性高血压肝阳上亢证、肝肾阴虚证病人正常活动状态下２４ｈ血压进行动态监测,分析两证型的血压变化规律。结果显示肝阳上亢证组血压呈昼高夜低、肝肾阴虚证组血压呈昼低夜高的规律性变化,表明２４ｈ动态血压监测值可作为原发必高血压辨证分型的客观指标之一。