Organ-specific acellular matrix for reconstruction of the urinary tract

In urology, replacement of organs or organ segments has proved problematic. Current techniques do not replicate complete organ function, and they cause well-known complications. With the acellular organ-specific matrix we have found a way to regenerate tissue components seen in the normal lower urinary tract. The time required for regeneration depends on the matrix size and function. The matrix is covered by urothelium migrating from the host, after which neovascularization occurs, followed by formation of smooth-muscle cells and nerves. In our studies, normal muscle lining and nerves providing functional tissue were demonstrable and no sign of antigenicity was evident, even after heterologous grafting. The regenerated rat bladder was evaluated by organ bath as well as by in vivo functional tests and demonstrated properties and functions similar to those of host tissue. Besides our obtaining encouraging results in the rat bladder, we also studied the organ-specific acellular matrix in other species (dog and rabbit) and other organ segments (ureter and urethra).     

Relative bioavailability of ketoprofen 20% in a poloxamer-lecithin organogel.

PURPOSE: The bioavailability of a single, topically applied, 200-mg dose of ketoprofen (delivered in a ketoprofen 20% gel) relative to a single 50-mg oral dose in healthy volunteers was studied. METHODS: This was an open-label crossover study. The subjects were randomized to receive an oral 50-mg ketoprofen capsule or a single topical dose of ketoprofen 20% in a poloxamer-lecithin organogel (PLO). Treatment was followed by a one-week washout period. Blood samples were collected at intervals up to 10 hours after administration, and plasma ketoprofen concentrations were determined by high-performance liquid chromatography with ultraviolet or mass spectrometry detection. Noncompartmental pharmacokinetic values were obtained after each dose, and relative bioavailability was calculated. RESULTS: Eight healthy volunteers enrolled in and completed the study. Topical absorption of ketoprofen was highly variable among the subjects over the 10-hour sampling period. The median oral maximum plasma concentration (Cmax) exceeded the topical Cmax by nearly 200-fold (4.15 versus 0.021 microg/mL) (p = 0.001). The median relative bioavailability of topical ketoprofen was 0.48%, with individual subjects' values ranging from 0.18% to 2.1%. CONCLUSION: The relative bioavailability of ketoprofen was low and highly variable when the drug was administered as a single dose in a PLO-based ketoprofen 20% gel.     

Trichodermamides A and B,cytotoxic modified dipeptides from the marine-derived fungus Trichoderma virens

Trichodermamides A (1) and B (2), two modified dipeptides, have been isolated from cultures of the marine-derived fungus Trichoderma virens. The trichodermamides possess a rare cyclic O-alkyl-oxime functionality incorporated into a six-membered ring. The structure of trichodermamide B was established by X-ray diffraction analysis, while the structure assignment of trichodermamide A, and determination of the absolute stereochemistry, was accomplished by spectral and chemical methods. Trichodermamide B displayed significant in vitro cytotoxicity against HCT-116 human colon carcinoma with an IC(50) of 0.32 microg/mL.     

Über die zur minimalen Erregung des menschlichen Geruchs- bzw. Geschmackssinnes notwendigen Molekülmengen

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Schnellmethode zur Beurteilung Der UrÄmie Als Ersatz Der Reststickstoff-Bestimmung

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Objektive und Subjektive Zeiteinheit

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THE OCCURRENCE OF ANTIGOITROUS SUBSTANCES IN PLANTS

The development of simple goiter in rabbits, which results when they are fed on a diet consisting chiefly of cabbage (or other Brassica) can be prevented by the feeding of certain fresh plants. Lawn grass, fresh alfalfa, skunk cabbage and steamed cabbage press juice have been found effective. Washing Brassicae with water improves their goiter-producing action, probably by the removal of goiter-preventing substances. The antigoitrous effect of plant juices was found to vary with the amount of reducing substance other than glutathione present. Skunk cabbage press juice, obtained from growing plants during the spring months, contained a large amount of iodine-absorbing material, and was very effective in involuting hyperplastic glands while juice obtained from mature plants in July when the iodine-absorbing material had nearly all disappeared, was quite ineffective. Certain extracts from cabbage administered parenterally, containing the reducing material, also had a marked involuting action on the thyroid. These extracts were practically iodine-free. The substance or substances believed to be responsible are easily soluble in water, ethyl alcohol, aqueous ethyl alcohol and are precipitable by lead acetate at a pH of 0.68–0.70. They are partially destroyed by exposure to air and by steam at 100°. Hexuronic acid isolated by Szent-Györgyi from cabbage, orange juice and suprarenal glands could be the antigoitrous agent. It cannot be glutathione.     

Der Gegenwärtige Stand der Lehre vom Chemismus der Zellen und Gewebe in Beziehung zur Pathologie der Allgemeinen Lipoidosen

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Einzelreferate und Buchbesprechungen

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Einzelreferate und Buchbesprechungen

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