全文获取类型
收费全文 | 17328篇 |
免费 | 1563篇 |
国内免费 | 39篇 |
专业分类
耳鼻咽喉 | 104篇 |
儿科学 | 544篇 |
妇产科学 | 465篇 |
基础医学 | 2490篇 |
口腔科学 | 316篇 |
临床医学 | 2222篇 |
内科学 | 3430篇 |
皮肤病学 | 357篇 |
神经病学 | 1655篇 |
特种医学 | 534篇 |
外科学 | 1777篇 |
综合类 | 211篇 |
一般理论 | 26篇 |
预防医学 | 2084篇 |
眼科学 | 345篇 |
药学 | 1196篇 |
中国医学 | 34篇 |
肿瘤学 | 1140篇 |
出版年
2023年 | 146篇 |
2022年 | 228篇 |
2021年 | 550篇 |
2020年 | 323篇 |
2019年 | 462篇 |
2018年 | 489篇 |
2017年 | 368篇 |
2016年 | 426篇 |
2015年 | 420篇 |
2014年 | 545篇 |
2013年 | 795篇 |
2012年 | 1169篇 |
2011年 | 1150篇 |
2010年 | 670篇 |
2009年 | 531篇 |
2008年 | 968篇 |
2007年 | 987篇 |
2006年 | 917篇 |
2005年 | 858篇 |
2004年 | 757篇 |
2003年 | 702篇 |
2002年 | 625篇 |
2001年 | 280篇 |
2000年 | 267篇 |
1999年 | 248篇 |
1998年 | 166篇 |
1997年 | 143篇 |
1996年 | 138篇 |
1995年 | 114篇 |
1994年 | 115篇 |
1993年 | 92篇 |
1992年 | 213篇 |
1991年 | 204篇 |
1990年 | 199篇 |
1989年 | 193篇 |
1988年 | 167篇 |
1987年 | 172篇 |
1986年 | 168篇 |
1985年 | 169篇 |
1984年 | 97篇 |
1983年 | 135篇 |
1982年 | 88篇 |
1981年 | 86篇 |
1980年 | 106篇 |
1979年 | 95篇 |
1977年 | 81篇 |
1976年 | 82篇 |
1975年 | 83篇 |
1974年 | 81篇 |
1973年 | 94篇 |
排序方式: 共有10000条查询结果,搜索用时 625 毫秒
141.
142.
Andrulis IL Anton-Culver H Beck J Bove B Boyd J Buys S Godwin AK Hopper JL Li F Neuhausen SL Ozcelik H Peel D Santella RM Southey MC van Orsouw NJ Venter DJ Vijg J Whittemore AS;Cooperative Family Registry for Breast Cancer studies 《Human mutation》2002,20(1):65-73
A number of methods are used for mutational analysis of BRCA1, a large multi-exon gene. A comparison was made of five methods to detect mutations generating premature stop codons that are predicted to result in synthesis of a truncated protein in BRCA1. These included four DNA-based methods: two-dimensional gene scanning (TDGS), denaturing high performance liquid chromatography (DHPLC), enzymatic mutation detection (EMD), and single strand conformation polymorphism analysis (SSCP) and an RNA/DNA-based protein truncation test (PTT) with and without complementary 5' sequencing. DNA and RNA samples isolated from 21 coded lymphoblastoid cell line samples were tested. These specimens had previously been analyzed by direct automated DNA sequencing, considered to be the optimum method for mutation detection. The set of 21 cell lines included 14 samples with 13 unique frameshift or nonsense mutations, three samples with two unique splice site mutations, and four samples without deleterious mutations. The present study focused on the detection of protein-truncating mutations, those that have been reported most often to be disease-causing alterations that segregate with cancer in families. PTT with complementary 5' sequencing correctly identified all 15 deleterious mutations. Not surprisingly, the DNA-based techniques did not detect a deletion of exon 22. EMD and DHPLC identified all of the mutations with the exception of the exon 22 deletion. Two mutations were initially missed by TDGS, but could be detected after slight changes in the test design, and five truncating mutations were missed by SSCP. It will continue to be important to use complementary methods for mutational analysis. 相似文献
143.
144.
145.
146.
Wells GD Plyley M Thomas S Goodman L Duffin J 《European journal of applied physiology》2005,94(5-6):527-540
The efficiency of the respiratory system presents significant limitations on the bodys ability to perform exercise due to the effects of the increased work of breathing, respiratory muscle fatigue, and dyspnoea. Respiratory muscle training is an intervention that may be able to address these limitations, but the impact of respiratory muscle training on exercise performance remains controversial. Therefore, in this study we evaluated the effects of a 12-week (10 sessions week–1) concurrent inspiratory and expiratory muscle training (CRMT) program in 34 adolescent competitive swimmers. The CRMT program consisted of 6 weeks during which the experimental group (E, n=17) performed CRMT and the sham group (S, n=17) performed sham CRMT, followed by 6 weeks when the E and S groups performed CRMT of differing intensities. CRMT training resulted in a significant improvement in forced inspiratory volume in 1 s (FIV1.0) (P=0.050) and forced expiratory volume in 1 s (FEV1.0) (P=0.045) in the E group, which exceeded the S groups results. Significant improvements in pulmonary function, breathing power, and chemoreflex ventilation threshold were observed in both groups, and there was a trend toward an improvement in swimming critical speed after 12 weeks of training (P=0.08). We concluded that although swim training results in attenuation of the ventilatory response to hypercapnia and in improvements in pulmonary function and sustainable breathing power, supplemental respiratory muscle training has no additional effect except on dynamic pulmonary function variables. 相似文献
147.
Rabon-Stith KM Hagberg JM Phares DA Kostek MC Delmonico MJ Roth SM Ferrell RE Conway JM Ryan AS Hurley BF 《Experimental physiology》2005,90(4):653-661
To determine the influence of the vitamin D receptor (VDR) gene FokI and BsmI genotype on bone mineral density response to two exercise training modalities, 206 healthy men and women (50-81 years old) were studied before and after approximately 5-6 months of either aerobic exercise training (AT) or strength training (ST). A totla of 123 subjects completed AT (51 men, 72 women) and 83 subjects completed ST (40 men, 43 women). DNA was extracted from blood samples of all subjects and genotyping was performed at the VDR FokI and BsmI locus to determine its association to training response. Total body, greater trochanter and femoral neck bone mineral density (BMD) were measured before and after both training programmes using dual-energy X-ray absorptiometry. VDR BsmI genotype was not significantly related to BMD at baseline or after ST or AT. However, VDR FokI genotype was significantly related to ST- but not AT-induced changes in femoral neck BMD (P < 0.05). The heterozygotes (Ff) in the ST group approached a significantly greater increase in femoral neck BMD (P = 0.058) compared to f homozygotes. There were no significant genotype relationships in the AT group. These data indicate that VDR FokI genotype may influence femoral neck BMD response to ST, but not AT. 相似文献
148.
Al-Maghrabi J Kamel-Reid S Jewett M Gospodarowicz M Wells W Banerjee D 《Archives of pathology & laboratory medicine》2001,125(3):332-336
CONTEXT: Primary lymphoma of the urinary bladder is rare. Only 84 cases have been reported in the English literature to date, and none of these cases has had molecular confirmation of clonal immunoglobulin gene rearrangement. OBJECTIVES: To review all cases with primary urinary bladder lymphoma in our records, to classify them using the REAL classification, to confirm their immunophenotype and genotype, and to determine their outcome. DESIGN: We identified 4 cases of primary urinary bladder lymphoma in our medical records from a 30-year period. Immunohistochemical detection of immunoglobulin light chains and molecular analysis of immunoglobulin heavy-chain genes using the polymerase chain reaction were performed on paraffin-embedded material. RESULTS: All patients were older than 60 years. The male-female ratio was 1:3. All patients had a history of chronic cystitis. Histologic features of mucosa-associated lymphoid tissue lymphoma with centrocyte-like cells, plasmacytoid B cells, or both were observed in all cases. Monoclonality of B cells was demonstrated by immunohistochemistry, polymerase chain reaction, or both methods in every case. All patients presented with stage IAE disease, were treated with radiotherapy alone, and have been in continuous complete remission for 2 to 13 years. CONCLUSIONS: Primary bladder lymphomas are usually of low-grade mucosa-associated lymphoid tissue type. They are more common in females and are associated with a history of chronic cystitis. Lymphoepithelial lesions are seen only in association with areas of cystitis glandularis. B-cell clonality is readily demonstrable by immunohistochemistry and/or polymerase chain reaction analysis. Local radiotherapy appears to confer long-term control. 相似文献
149.
A comparison of three months of anticoagulation with extended anticoagulation for a first episode of idiopathic venous thromboembolism 总被引:30,自引:0,他引:30
Kearon C Gent M Hirsh J Weitz J Kovacs MJ Anderson DR Turpie AG Green D Ginsberg JS Wells P MacKinnon B Julian JA 《The New England journal of medicine》1999,340(12):901-907
BACKGROUND: Patients who have a first episode of venous thromboembolism in the absence of known risk factors for thrombosis (idiopathic thrombosis) are often treated with anticoagulant therapy for three months. Such patients may benefit from longer treatment, however, because they appear to have an increased risk of recurrence after anticoagulant therapy is stopped. METHODS: In this double-blind study, we randomly assigned patients who had completed 3 months of anticoagulant therapy for a first episode of idiopathic venous thromboembolism to continue receiving warfarin, with the dose adjusted to achieve an international normalized ratio of 2.0 to 3.0, or to receive placebo for a further 24 months. Our goal was to determine the effects of extended anticoagulant therapy on rates of recurrent symptomatic venous thromboembolism and bleeding. RESULTS: A prespecified interim analysis of efficacy led to the early termination of the trial after 162 patients had been enrolled and followed for an average of 10 months. Of 83 patients assigned to continue to receive placebo, 17 had a recurrent episode of venous thromboembolism (27.4 percent per patient-year), as compared with 1 of 79 patients assigned to receive warfarin (1.3 percent per patient-year, P<0.001). Warfarin resulted in a 95 percent reduction in the risk of recurrent venous thromboembolism (95 percent confidence interval, 63 to 99 percent). Three patients assigned to the warfarin group had nonfatal major bleeding (two had gastrointestinal bleeding and one genitourinary bleeding), as compared with none of those assigned to the placebo group (3.8 vs. 0 percent per patient-year, P=0.09). CONCLUSIONS: Patients with a first episode of idiopathic venous thromboembolism should be treated with anticoagulant agents for longer than three months. 相似文献
150.
Seventy-six patients were interviewed within a week of admission following a parasuicide episode. Axis II diagnosis on DSM-III was made for schizotypal, borderline, histrionic, and antisocial personality disorder. In addition patients completed a self-rating questionnaire, the Schizotypy Questionnaire of Claridge & Broks (1984), which assesses schizotypal and borderline personality traits. The objective and subjective indices of schizotypal and borderline symptoms correlated significantly but allocation of patients to a diagnosis missed several patients who nevertheless rated themselves as having a high frequency of these symptoms. There was an asymmetry of symptom pattern reminiscent of Foulds & Bedford's (1975) hierarchy model. The presence of schizotypal symptoms appeared to be higher in the hierarchy: they predicted borderline symptoms, but a high frequency of borderline symptoms did not necessarily predict schizotypy. We suggest that the occurrence of schizotypal symptoms should become a more explicit focus of clinical assessment and treatment of these patients, especially those who repeatedly harm themselves and we suggest ways in which cognitive therapies may be adapted to do this. 相似文献