尼群地平、依那普利与牛磺酸合并应用对肾性高血压大鼠降压的协同研究

尼群地平１０ｍｇ·ｋｇ－１、依那普利１０ｍｇ·ｋｇ－１和牛磺酸５０ｍｇ·ｋｇ－１一次口服对肾性高血压大鼠均有降压作用，三者降压作用强度相当，最大降压百分率（给药后６ｈ）分别为３３、３６．６和２５．１，降压持续时间相似，给药后２４ｈ的降压百分率分别为１４．２、４．８和１３．７。尼群地平，依那普利分别与牛磺酸以及尼群地平与依那普利合用均有协同降压作用，Ｑ值分别为２．２１、２．６７和１．６４，且降压作用持久，给药后２４ｈ的降压百分率分别为３９．６、２６．８和３８．８，显著高于各药单用。     

牙源性脓肿标本中的产黑色素类杆菌群菌株分离鉴定

本文对４４例牙源性脓肿（尖周脓肿２４例，牙周脓肿１２例，冠周脓肿８例）脓液标本中的产黑色素类杆菌群菌株进行分离、培养、鉴定。其中３８例存在产黑菌群，以中间型类杆菌阳性率最高。牙龈类杆菌在牙周脓肿中检出率最高。２４例尖周脓肿可分离出６株牙髓类杆菌而其它两种牙源性脓肿则无牙髓类杆菌，推测该菌与牙髓尖周感染的病理过程有关。     

用下睑眼轮匝肌蒂瓣与鼻唇沟皮瓣一次修复下睑全层缺损三例

用下睑眼轮萜肌蒂瓣与鼻唇沟皮瓣一次性修复下睑全层缺损三例。男性１例，女性２例，平均年龄５０岁，均为下睑１／２￣１／３全层缺损。基底细胞癌、睑板腺癌、鳞状细胞癌各１例，皮瓣全部成活。术后随访１年，下睑外形良好，闭眼功能正常。     

住院医师临床技能评估方法探讨

该文总结了安徽省立医院住院医师培训情况,提出了必须引入实践性考核作为住院医师临床技能考核的主要方式。根据临床技能评价的项目,制定了内科、外科、影像科住院医师临床技能评估指标体系,确立了临床技能考核的原则,并组织实施。这种评估方法能全面考核住院医师的临床技能,但在新的医疗形势下,需建立相关配套措施来保证实施。     

阿霉素不同剂量静脉注射的药动学及其临床意义

采用ＨＰＬＣ法测定１４例肿瘤患者使用不同剂量阿霉素的血药浓度，并计算药代参数。４０ｍｇ／ｍ２和２５ｍｇ／ｍ２两组的血药峰浓度、ＡＵＣ、Ｖｃ差异有显著性。阿霉素的药代动力学存在明显的个体差异，血药峰浓度、Ｖｃ、Ｋ１２与疗效相关。     

大肠癌细胞增殖分化与多药耐药性的关系

目的 :探讨大肠癌细胞增殖、分化与肿瘤细胞耐药性之间的关系。方法 :收集大肠癌标本 51例 ,显微镜下观察肿瘤的分化情况 ,并用免疫组织化学ABC法检测肿瘤细胞增殖细胞核抗原 (PCNA)和P 糖蛋白 (P Glycoprotein ,P gp)的表达情况 ,分析肿瘤细胞增殖、分化与Pgp表达的关系。 结果 :不同类型和分化程度的肿瘤细胞PCNA和Pgp的表达不同 ,但是 ,它们相互之间无明显的相关性 (P >0 .0 5)。结论 :大肠癌细胞增殖、分化与耐药性的产生分别为独立的现象     

Identification of Leishmania donovani isolates from different kala-azar foci in China by kDNA hybridization.

kDNA sequence homology of Leishmania donovani isolates from three types of kala-azar foci in China were analyzed by using dot and Southern hybridization with biotin- and 32P-labelled probes. The results revealed kDNA sequence heterogeneity among Leishmania donovani isolates from the three kala-azar foci: sequence homology between isolates of hill and desert foci was higher than that between hill and plain foci isolates. The kDNA hybridization technique was also found to be specific and sensitive for direct identification of Leishmania in animal tissues. In a preliminary survey, kDNA hybridization of cutaneous tissue blots of 71 dogs from endemic regions showed a positive rate of 40.8%, and the rate of double positive cases (touch blot hybridization and bone marrow smear) reached 91.3%. The direct identification of Leishmania in tissues by kDNA hybridization seems to be a useful and convenient method for epidemiological study and clinical diagnosis, especially for species/strain characterization.     

单片机自动灌流系统设计及其医学应用

介绍一种用单片机构成的自动灌流控制系统。它以灌流压为控制指标，以ＬＤＢ－Ｍ电子蠕动泵作为执行机构，构成闭环控制系统，能实现用户设定的各种液流压曲线。系统内采用了ＥＥＰＲＯＭ存储器，可对用户设定的多达１０条灌流曲线进行长期保存。     

低温洗涤红细胞肺保护液对体外循环术后肺保护作用的研究

目的:探讨体外循环(cardiopulmonarybypass,CPB)术中采用低温洗涤红细胞肺保护液实施肺动脉灌注对肺的保护效果。方法:随机选取20例风湿性单纯二尖瓣病变合并中度肺动脉高压的患者,分为肺动脉灌注组和对照组(每组各10例)。灌注组在主动脉阻断后,经肺动脉根部间断灌注低温洗涤红细胞肺保护液,测定两组患者围术期的机械通气时间、肺血管阻力、白细胞比值(静脉血/动脉血)、肺循环血浆MDA含量。结果:体外循环术后灌注组机械通气时间显著低于对照组(P<0.05);各时点肺血管阻力、白细胞比值(静脉血/动脉血)、肺循环血浆MDA含量均显著低于对照组(P<0.01)。结论:低温洗涤红细胞肺保护液肺动脉灌注对体外循环术后肺损伤有明显的保护作用.     

Antibodies against dengue virus E protein peptide bind to human plasminogen and inhibit plasmin activity

Both mice and rabbits immunized with dengue virus E protein peptide spanning amino acids 100–119 (D4E) produced antibodies that reacted not only with the D4E peptide itself but also with human plasminogen, as shown by ELISA and Western blot. Sera from dengue virus-hyperimmunized mice and dengue patients also contained antibodies against D4E and plasminogen. Furthermore, such sera all contained plasmin inhibitory activity. Using affinity-purified anti-D4E antibodies and free D4E peptide for competitive inhibition, we demonstrated that the inhibition of plasmin activity was due to anti-D4E antibodies rather than other substances in the sera. Taken together, these results suggest dengue virus E protein amino acids 100–119 are a cross-reactive immunogenic region, and antibodies against this region may interfere with human fibrinolysis.