Experimental study on inhibitory effect of bridgy duct of hepatic artery on apoptosis of liver and bile duct cells

Objective： To investigate whether the method of bridgy duct established between the recipient＇s spleen artery and the donor＇s gastroduodenal artery could inhibit the apoptosis of liver and bile duct cells caused by hepatic artery ischemia. Methods： Twenty-four mongrel dogs from Xi＇an area were used to establish simplified models of dog orthotopic liver transplantation and divided into three groups randomly： HAI group （hepatic artery ischemia group）,BBB group（bypassing the blood by a bridgy duct） and control group. After cold perfusion, The samples were collected from liver and bile duct in each group at different time and fixed in glutaraldehyde and 4% polyformaldehyde respectively. At last, the apoptosis of liver and bile duct cells were observed and the apoptotic indexes were calculated. Results： Two hours after cold perfusion, apoptotic phenomenon was common in HAI group, rare in BBB group, while no apoptotic phenomenon was observed in control group. TUNEL staining showed that there was no significant difference in apoptotic index among the three groups immediately after cold perfusion. However, with time going, the apoptotic cells were increased in three groups, and the difference in apoptotic index was significant among three groups （P 〈 0.01 ）. Conclusions： Bridgy duct of hepatic artery can inhibit the apoptosis of liver and bile duct cells caused by HAI significantly.     

Influences of V5-epitope tag on the metabolic activation of AFB1 by human cytochrome P450 2A13

INTRODUCTION A flatoxin B1 (AFB1) , which is produced by Aspergillus flavus and Aspergillus parasitus, is a potent carcinogen in human and animals. Al- though AFB1 is best known as a liver carcinogen, it also induces lung tumors in animals and epidemio- logical studies show a positive association between human lung cancer occurrence and inhalation expo- sure to AFB1-containminated grain dust. As most of environmental carcinogens, metabolic activation cat- alyzed by cytochrome P450 (C…     

不同性别大鼠创伤失血性休克后多形核白细胞反应的差异及胃复安干预的实验研究

本研究在以下几个方面具有创新：（1）将腺苷引入心肺复苏治疗中，观察腺苷与肾上腺素合用对心跳停搏后A主循环恢复率和复苏成功率的影响，结果表明合用腺苷可明显提高自主循环恢复率和复苏成功率；（2）观察腺苷在心肺复苏中对兔心、脑组织病理结构和超微结构的影响，结果表明腺苷可以明显减轻心脑组织的损害；（3）观察腺苷窒息大鼠心脏和肠系膜微循环的影响，并观察到肾上腺素对微循环无明显改善作用，联合应用腺苷可以改善微循环；（4）将激光多普勒血流量图象仪引入心肺复苏中，动态观察窒息大鼠肠壁组织血液灌流量变化，并观察到腺苷可以增加肠壁组织血流灌注量。[编者按]     

Induction of apoptosis and inhibition of proliferation in Hep-2 by antisense survivin RNA in vitro

Objective.. To study induction of apoptosis and inhibition of proliferation in Hep-2 by antisense survivin RNA. Methods： Antisense survivin RNA expression vector was constructed and then was transfected to human laryngeal carcinoma cell line Hep-2 by lipofectamine. HpEGFP/survivin cells （transfected with the combinant of antisense survivin RNA） were obstained by using G418. The levels of survivin protein before and after transfection were determined by Western-blot. Proliferation activity was measured by MTT assay. The experiment of colony formation in soft agar was carried out for assessing ability of proliferation of Hep-2 cell. Apoptosis was assessed by flow cytometry and acrdine orange（AO）. Results： After antisense survivin RNA plasmids were transfected, the level of survivin protein was inhibited in Hep-2. Compared with control, proliferation of HpEGFP/survivin cells were suppressed significantly. The experiment of colony formation in soft agar showed the ability of colony formation decreased in HpEGFP/survivin cells compared to control （P〈0.05）. Apoptosis rate increased about 1.81-folds compared with control. Conclusion.. The antisense survivin RNA can partly inhibit the level of surviivin protein expression in Hep-2 and can induce apoptosis and inhibit the proliferation of Hep-2 by down-regulating the expression of endogenous survivin in vitro.     

急性胰腺炎128例临床综合治疗结果分析

目的 观察应用ICU监护治疗急性胰腺炎的临床效果。方法 2004年1月。2005年12月共经ICU监护治疗的急性胰腺炎患者27例（治疗组）。非ICU监护治疗（建立ICU前）的急性胰腺炎患者101例（对照组），两组患者从性别、年龄、治疗效果、手术例数、临床指标恢复正常时间分别进行分析统计。结果 无论MAP或SAP，治疗组和对照组从疗效、手术例数、临床指标恢复正常时间三方面进行比较：治疗组疗效优于对照组。治疗组手术患者例数、临床指标恢复正常时间都少于对照组。结论 通过ICU监护治疗急性胰腺炎是一种行之有效的处理方法。     

防石方防治内镜微创保胆取石术后胆囊结石复发的临床观察

目的探讨中药防石方防治内镜微创保胆取石术后胆囊结石复发的临床疗效.方法将我院2000～2002年符合内镜微创保胆取石术条件的120例胆囊结石患者分为对照组、观察组各60例,两组均先行内镜微创保胆取石术,对照组术后服消炎利胆片治疗,观察组术后服防石方治疗,比较两组疗效.结果观察组、对照组3年内胆囊结石复发率分别为1.67%、13.33%;观察组慢性胆囊炎症状消失较对照组理想(P＜0.05),有显著性意义.结论防石方减少内镜微创保胆取石术后胆囊结石复发、减轻慢性胆囊炎症状的功效较消炎利胆片为优.     

螺旋CT虚拟内诊镜在小儿气管支气管异物诊断中的应用

目的探讨螺旋CT虚拟内诊镜(CTVE)在小儿气管、支气管异物诊断上的应用价值。 方法回顾性分析东南大学医学院附属徐州医院1998年1月至2004年12月收治的121例术前经CTVE诊断为气管支气管异物，并行支气管镜探查术患儿的诊疗情况，并分析CTVE诊断在临床应用上的意义及诊断的阳性符合率。 结果121例经CTVE诊断并行支气管镜探查证实并取除异物者115例，诊断符合率9504%。 结论CTVE检查是目前无创非侵入性诊断小儿气管支气管异物的较理想方法。     

注意缺陷多动障碍诊断量表父母版的中国城市儿童常模制定及其信度和效度的检验

目的建立父母用注意缺陷多动障碍诊断量表(ADHDDS P)的中国城市儿童常模，并检验其信度和效度。 方法中南大学湘雅二院精神卫生研究所等单位于2004 08—2005 04在全国12个城市采样1616例(男777例，女839例)，年龄6~17(1110±280)岁，由父母填写ADHDDS P。 结果常模样本男性在注意缺陷、多动冲动分量表和总分都高于女性；注意缺陷分量表在各年龄之间差异无显著性意义，多动冲动分量表和总分6~8岁得分最高，随年龄增加而得分递减。信度：间隔4周量表总分的重测信度r=072;Crobachａ系数091;项目与总分的一致性r为061~071；父母和教师之间的一致性032。效度：与Conners父母症状问卷的多动指数(r=075)，CBCL的注意问题(r=065)、外化性问题(r=065)相关；ADHD儿童的得分显著高于常模组；以常模第93百分位作为划界分，对ADHD诊断的灵敏度为092，特异度为090。因子分析提取三因素模型：注意缺陷、多动和冲动。 结论ADHDDS常模分布存在年龄和性别差异，其信度效度达到心理测量学要求，可用于我国儿童ADHD辅助诊断。     

56例儿童IgA肾病预后的影响因素分析

目的探讨儿童IgA肾病预后及其临床、肾脏病理的影响因素。方法将56例随访时间至少1年的IgA肾病患儿根据其随访终点时临床表现分为A、B、C、D4种结局作为预后评估指标,再回溯起病时临床和病理资料,进行统计分析。结果治疗时间、尿蛋白定量、临床分型为Ⅲ型(肾病综合征)为影响预后的3个主要临床因素(P<0.05);肾小球积分和球性硬化积分是影响预后的重要肾脏病理因素(P<0.05);免疫病理与预后无关。结论蛋白尿是影响儿童IgA肾病预后的主要临床因素,坚持长程治疗对改善预后有益;应用Katafuchi半定量评分对儿童IgA肾病肾脏损害程度进行评估具有更大的优越性,肾小球积分和球性硬化积分增高是预后不良的高危病理因素。     

医源性输尿管损伤和狭窄22例临床分析

目的提高医源性输尿管损伤和狭窄的警惕性和认识。方法对22例医源性输尿管损伤和狭窄的原因及处理方法进行总结。结果医源性输尿管损伤16例中,妇科手术10例(10/1663%),其中6例患者术中得到及时发现处理,4例因术后一周内一侧腰部坠胀行IVU检查患侧不显影而发现,行输尿管膀胱再植术;1例宫颈癌术后一年后因尿毒症就诊。乙状结肠癌根治手术4例(4/1625%),其中有2例给予患侧肾切除,半年后均死于原发性疾病;泌尿外科2例(2/1612%)由于泌尿外科逆行插管穿孔,给予观察未出现不良后果;7例医源性狭窄有6例由泌外手术导致,3例得到再次手术,2例拒绝进一步治疗。结论手术者应充分了解下段输尿管的解剖,以免造成输尿管医源性损伤;过分强调抗逆流可能是导致输尿管狭窄的主要原因。