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41.
伤寒史学研究成果简述 总被引:1,自引:0,他引:1
万晓刚 《Zhonghua yi shi za zhi (Beijing, China : 1980)》2000,30(4):211-214
建国以来有关伤寒史学的研究成果表现在相关人物、相关著作、相关事件、学术关系及阶段特征五个方面。前期研究成果 ,尚不足以充分展示伤寒学历史地位和现实意义 ,更无充分依据昭示其未来之发展方向 ,因而有必要继续深入开展伤寒史学研究 相似文献
42.
柱切换HPLC法测定腹水中左旋氧氟沙星的含量 总被引:1,自引:0,他引:1
目的:应用柱切换HPLC技术直接测定腹水中左旋氧氟沙得含量。方法:采用磷酸二氢钾缓冲溶液(PH2.2)-0.05mol/L四丁溴化化铵(100:4)为预处理流动相,经μBondapak C18柱预处理后,切换到Irregular_HC18分析柱,以甲醇-磷权二氢钾缓冲溶液(PH2.2)-0.05mol/L四丁基溴化铵(30:70:4)为分析流动相进行分离测定,紫外线皮长为294nm。结果:左旋氧氟 相似文献
43.
The mutation of insulin receptor substrate-1 gene in Chines e patients with non-insulin-dependent diabetes mellitus 总被引:6,自引:0,他引:6
Objective To identify the relationship between mutation in the insulin receptor substrate-1 (IRS-1) gene and the incidence of non-insulin-dependent diabetes mellitus (NIDDM) in the Chinese population. Methods Samples were obtained from 68 Chinese patients with NIDDM and 68 control subjects. The +1700~+4437 bp fragment of the IRS-1 gene was screened by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. All SSCP variations were submitted to DNA sequence analysis. Results Two amino acid variations [GGG →AGG(G(971) R) and CCT→TCT(P(1079) S)] and 3 silent mutations [GAT→GAC(D(422) D), CCA→CCC(P(737) P) and GCA→GCG(A(804) A)] were identified, among which the CCA→CCC(P(737) P) and CCT→TCT(P(1079) S) have not been previously reported. All five variations were found in Chinese patients with NIDDM, while GCA→GCG(A(804) A) was the only one found in control subjects. The overall incidence of the five variations in Chinese patients with NIDDM were much higher than that in control subjects (38.2% vs 7.4%, χ(2)=18.42,P<0.01). The most common polymorphism in the Chinese population was GCA→GCG(A(804) A), and its frequency was significantly higher in Chinese patients with NIDDM than in controls (26.5% vs 7.4%, χ(2)=8.84,P<0.01). The homozygotes of the variation in patients with NIDDM and control subjects were 8.8% and 1.5%, respectively (χ(2)=2.41,P>0.05). Conclusion These results indicate that there may be a relation between these nucleotide variations of IRS-1 gene and Chinese patients with NIDDM 相似文献
44.
Objective To evaluate the ability of the RIFLE classification to predict hospital mortality in adult patients who underwent cardiac surgery. Methods From October Ist 2006 to December 31st 2006, five hundred and nine adult patients who underwent coronary artery bypass grafting and/or valve operation were enrolled in this study. Renal function was assessed daily according to the RIFLE classification, meanwhile, APACHE Ⅱ score and SOFA score were also evaluated, as well as the maximum scores were recorded. Results Mean duration of ventilation support was 18(14 - 19) hours, the time of ICU stay was 1.4 ± 1.0 days, and the time of postoperative hospital stay was 12. 0(10.0- 15.0) days. 167 patients (32. 8%) incurred postoperative ARF according to the RIFLE classification. The overall mortality was 4. 3% (22/502). A significant increase (P < 0. 01) was observed for mortality based on RIFLE classification. By applying the area under the receiver operating characteristic curve, the RIFLE classification had more powerful discrimination power [0. 933, (95% CI 0. 872 -0. 995) ,P <0. 001]. Conclusions ARF is one of the major complications in postcardiotomy patients. Analytical data suggested the good discriminative power of the RIFLE classification for predicting inpatient mortality of adult postoperative patient with ARF, and the RIFLE classification is simple and practically performed. According to the RIFLE classification, patients with RIFLE class I or class F incur a significantly increased risk of in-hospital mortality compared with those who never develop ARF. 相似文献
45.
目的 了解成人急性散发性病毒性肝炎的病原学分型及庚型肝炎病毒感染状况。方法 利用ELISA和逆转录聚合酶链反应对病人血清进行检测。结果 成人急性肝炎中以乙型肝炎所占比例最大,占37.16%;其次甲型肝炎占18.92%,另有18.24%为非甲~庚型肝炎。结论 庚型肝炎病毒存在明显的重叠感染,与乙型和丙型肝炎病毒重叠感染率较高。 相似文献
46.
47.
小鼠肥大细胞瘤P815特异抗原P1A的克隆与表达 总被引:6,自引:1,他引:5
目的 克隆小鼠肥大细胞瘤P815细胞株的PIA基因以制备肿瘤DNA疫苗,方法 用RT-PCR方法制备PIA基因,以哺乳细胞高效表达质粒pCI-neo为载体,构建重组DNA疫苗。重组体用克隆位点上游和下游的T7和T13启动了序列为测序引物,用自动测序仪测定鉴定克隆的正确性。再将鉴定过的重组质粒用磷酸钙法转化293细胞,用RT-PCR法鉴定转化细胞中PIA基因的表达。结果 正确构建了PIA/pCI-n 相似文献
48.
50.
模拟飞行缺氧与吸氧对小鼠脏器自由基代谢的影响 总被引:4,自引:3,他引:1
目的 观察了两种模拟飞行条件(1500m轻度低压缺氧和5500m中度低压吸氧重复暴露下小鼠多脏器自由基代谢变化的特点。方法 昆明种小鼠60只,随机分为6组(n=10)进行缺氧与吸氧实验,实验完毕后,取小鼠尾血做血常规检查。次日,将小鼠断头处死,取脑、心、肺、肝、肾制备匀浆,测定丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 1500m轻度低压缺氧重复暴露8wk使肺MDA含量显著增高,提示 相似文献