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噬菌体变色法检测结核分枝杆菌 总被引:1,自引:0,他引:1
目的建立一种结核分枝杆菌快速检测方法。方法痰标本常规处理,离心集菌,取100μl于试管中,另一支加入标准株H37Rv结核分枝杆菌作阳性对照。两支试管分别加入噬菌体D29,37℃培养,用硫酸亚铁铵杀死结核分枝杆菌菌体外的噬菌体,柠檬酸三钠和氯化钙混和液中和硫酸亚铁铵。加入耻垢分枝杆菌和硝酸钾,37℃培养过夜,加入显色剂显色。同时用罗氏培养方法对照检测。结果临床标本的噬菌体变色法和罗氏培养法表明两方法结果具有一致性(P0.05)。结论噬菌体变色法可作为结核分枝杆菌的快速检测方法之一。 相似文献
383.
针灸治疗脑卒中痉挛性偏瘫的临床研究进展 总被引:1,自引:0,他引:1
近年来,脑卒中后痉挛性偏瘫的治疗受到重视,针灸作为一种有效的方法报道较多,本文总结了近年来关于针灸治疗脑卒中后痉挛性偏瘫的现状和进展,从取穴、针法、针灸结合其他方法等方面进行综述,认为针灸能够缓解脑卒中后痉挛性瘫痪状态,而且疗效好、无不良反应,并提出优化选穴、规范临床试验设计、统一疗效标准、进行机制研究等建议。 相似文献
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目的 探讨门诊护士定期进行晨间集体交接班制的实施方法和效果.方法 于每周一工作前30 min,全体门诊护士集中进行交接班,由交班护士、护士长及科护士长对上周工作进行总结及点评.结果 实施门诊护士定期晨间集体交接班制后门诊满意度显著提高,与实施前比较,差异有显著性意义(P<0.01).结论 门诊护士定期晨间集体交接班的实施,对培养门诊护士的综合素质及提高门诊护理服务质量起到积极的促进作用. 相似文献
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Cemal ORHAN Füsun ERTEN Beir ER Mehmet TUZCU Nurhan AHN
znur Ece DURMAZ KURUN Vijaya JUTURU Kazim AHN 《Turkish Journal of Medical Sciences》2021,51(4):2167
Background/aim This study was conducted to elucidate the effects of lutein/zeaxanthin isomers (L/Zi) on lipid metabolism, oxidative stress, NF-κB/Nrf2 pathways, and synaptic plasticity proteins in trained rats.Materials and methods Wistar rats were distributed into four groups: 1) control, 2) L/Zi: rats received L/Zi at the dose of 100 mg/kg by oral gavage, 3) exercise, 4) exercise+L/Zi: rats exercised and received L/Zi (100 mg/kg) by oral gavage. The duration of the study was eight weeks. Results Exercise combined with L/Zi reduced lipid peroxidation and improved antioxidant enzyme activities of muscle and cerebral cortex in rats (p < 0.001). In the Exercise + L/Zi group, muscle and cerebral cortex Nrf2 and HO-1 levels increased, while NF-κB levels decreased (p <0.001). Also, L/Zi improved BDNF, synapsin I, SYP, and GAP-43 levels of the cerebral cortex of trained rats (p < 0.001). The highest levels of BDNF, synapsin SYP, and GAP-43 in the cerebral cortex were determined in the Exercise+L/Zi group.Conclusion These results suggested that exercise combined with L/Zi supplementation might be effective to reduce neurodegeneration via improving neurotrophic factors and synaptic proteins, and oxidative capacity in the cerebral cortex. 相似文献
388.
Abdussamet BATUR Abidin KILINER Fatih ATE Nazlm Aktu DEMR Recai ERGÜN 《Turkish Journal of Medical Sciences》2021,51(3):972
Background/aimTo investigate the changes in the spleen size, parenchymal heterogeneity, and computed tomography (CT) texture analysis features of patients diagnosed with Coronavirus disease 2019 (COVID-19)Materials and methodsThe size and parenchymal structure of the spleen in 91 patients who underwent thoracic CT examination due to COVID-19 were evaluated. For the evaluation of parenchymal heterogeneity, CT texture analysis was performed using dedicated software (Olea Medical, France). The texture analysis of each case consisted of 15 first-order intensity-based features, 17 gray level co-occurrence matrix-based features, and 9 gray level run length matrix-based features.ResultsA total of 91 patients (45 males, 46 females) with a mean age of 54.31 ± 16.33 years (range: 18–81) were included in the study. A statistically significant decrease in spleen size was seen in the follow-up CT examinations (p < 0.001) whereas no statistically significant difference was found between the Hounsfield unit (HU) values. The radiomics consisted of first-order intensity-based features such as 90th percentile, maximum, interquartile range, range, mean absolute deviation, standard deviation, and variance, all of which showed statistically significant differences (p - values: < 0.001, < 0.001, 0.001, 0.003, 0.001, 0.001, and 0.004, respectively). “Correlation” as a gray level co-occurrence matrix-based feature and “gray level nonuniformity” as a gray level run length matrix-based feature showed statistically differences (p-values: 0.033 and < 0.001, respectively).ConclusionsAlthough COVID-19 manifests with lung involvement in the early stage, it can also cause systemic involvement, and the spleen may be one of its target organs. A decrease in the spleen size and parenchymal microstructure changes can be observed in the short follow-up time. It is hoped that the changes in the parenchymal microstructure will be demonstrated by a noninvasive method: texture analysis. 相似文献