血浆弹性蛋白酶的微量测定和临床应用

目的　拟建立一种血浆弹性蛋白酶的快捷、微量测定方法。方法　以琥珀酰甘氨酰对硝基苯胺 (succinyl-L -trianalinyl-p -nitroanilide ,SLAPN)作为弹性蛋白酶的作用底物 ,根据单位时间内 4-硝基苯胺释放量 (A450nm)的变化 ,反应弹性蛋白酶的活性。结果　线性范围为 0～ 5 0 0 μg/L,批内和批间误差分别为 5 .0 2 %和 8.3 2 % ,血浆用量仅为 2 0～ 40 μl。正常健康人 (n =12 )血浆弹性蛋白酶含量 (3 7.5 0± 15 .5 6)μg/L。肠瘘合并感染患者 (n =6)为 (4 8.40± 2 7.82 )μg/L,明显高于正常人 (P <0 .0 1)。猪急性出血坏死性胰腺炎 (5 %牛磺胆酸钠 + 80 0 0～ 10 0 0 0u/ml胰蛋白酶 )诱导12h后为 (2 3 9.0 6± 180 .2 4) μg/L明显高于诱导前水平〔(3 6.15± 9.40 ) μg/L,P <0 .0 1〕。结论　该方法操作简便、快捷、灵敏度高、特异性好 ,易推广普及     

学习记忆能力下降大鼠神经细胞增殖的研究

目的　观察学习记忆能力减退老年大鼠海马齿状回神经细胞的增殖。方法　Morris水迷宫筛选出学习记忆能力减退大鼠 (痴呆组 )与正常大鼠 (对照组 )。BrdU标记海马齿状回增殖细胞。TUNEL法标记DNA片段原位检测凋亡细胞。计数海马颗粒细胞层与海马门的BrdU阳性细胞与凋亡细胞数。结果　痴呆组与对照组大鼠相比 ,海马颗粒细胞层BrdU阳性细胞显著增加 (P <0 0 1) ,而凋亡细胞数无显著差异 (P >0 0 5 )。海马门BrdU阳性细胞数及凋亡细胞数均无显著差异 (P >0 0 5 )。结论　学习记忆能力减退的老年大鼠海马齿状回神经细胞增殖能力减低。     

合欢皮中新皂甙的结构鉴定

从合欢皮(Albizziae cortex)的95%乙醇提取物的正丁醇萃取部分中分离得到3个新的三萜皂甙,用化学方法及¹H-和¹³C-NMR,DEPT,COSY,TOCSY,HMQC-COSY,HMQC-TOCSY,HMBC,NOESY等波谱方法鉴定其结构为:I(1个三萜,9个糖,2个单萜),命名为合欢皂甙(julibroside)J₁;II(1个三萜,8个糖,2个单萜),命名为合欢皂甙J_{2;II(1个三萜,9个糖,2个单萜),命名为合欢皂甙J3。}     

尖锐湿疣患者外周血淋巴细胞凋亡调控蛋白的表达和血清中白细胞介素2水平的研究

为研究尖锐湿疣（CA）患者外周血淋巴细胞（PBLC）凋亡调控蛋白表达水平和血清中白介素2（IL－2）水平在CA免疫发病机制中的作用。采用流式细胞仪及间接免疫荧光素标记法和ELISA法，检测了60例不同病程CA患者PBLC凋亡调控蛋白Fas/Fas-L的表达水平和血清中IL－2水平。发现CA短病程组PBLC的Fas、Fas-L表达水平均明显高于健康对照组（P＜0.05)，而CA长病程组PBLC的Fas、Fas-L表达水平明显高于CA短病程组（P＜0.05)和健康对照组（P＜0.01)，短、长病程组CA患者血清IL－2的水平明显低于健康对照组（P＜0.01)，CA患者PBLC Fas/Fas-L表达水平与血清中IL－2的水平呈负相关（P＜0.01)。结果表明：CA患者PBLC有凋亡异常，并且与血清中IL－2的水平呈负相关，患者PBLC凋亡异常在CA的病程中可能起重要作用。     

Birth spacing patterns and correlates in Shaanxi, China

This study shows that most women in Shaanxi Province, China try to have their first birth as soon as possible after their first marriage, and that the length of the interval between marriage and first birth is strongly correlated with the woman's age at first marriage. The length of the second and third birth intervals and the likelihood of going on to have a second or third birth are strongly influenced by the sex composition of children already born, the survival time of the child initiating the interval, the duration of breastfeeding, and the woman's occupation. There is significant regional variation in the length of birth intervals and in the prevalence of second and third births in Shaanxi. The findings indicate that China's one-child policy is far from being universally accepted in Shaanxi, including its urban areas. The persistence of many social, economic, institutional, and cultural factors promoting high fertility poses many obstacles to further fertility decline.     

重症肌无力症134例放射治疗的疗效分析

本文回顾分析我院自1961年5月至1985年12月重症肌无力症134例主要采用胸腺放疗的结果。其中25例用深部经X线照射,109例~(60)Co放疗。随访时间2月至24年。完全缓鲜26例(19.5％),显著好转63例(47％),合计显效率为66.5％。56例仅接受放疗或同时服用抗胆碱酯酶剂,可以作为组内自身对照,证实放疗疗效较好。胸腺区或脾区放疗后患者血清nAchRab及淋巴细胞总数降低,具有抗淋巴细胞作用,能抑制自身免疫应答。如今放射治疗对MG已经不是经验性治疗,而是一种值得推荐的免疫调整治疗方法。本文初步提出治疗适应证。     

Down-regulation of Cardiac Bradykinin B2 Receptors and eNOs mRNA in Rats with Remnant Kidneys

Bradykinin,ani mportant substance in the de-velopment of cardiovascular diseases ,exerts its ac-tions through the activation of B1 or B2 receptors .B1 receptors are expressing mainly in pathologicalconditions and mediate inflammatory and pain-pro-ducing effects[1];B2 receptors(BKB2Rs) mediatemost of cardiovascular protective effects throughreleasing nitric oxide (NO) ,prostaglandins (PGs)and endothelium-derived hyperpolarizing factor(EDHF)[2]. The components for the generationand action …     

心律紊乱与心肌超微结构

对40例心律紊乱怀疑病毒性心肌炎(VMT)患者行心内膜心肌活检术(EMB)标本电镜进行超微结构观察,结果40例心律紊乱患者中,诊断为活动性心肌炎24例,早期扩张性心肌病8例,扩张性心肌病5例,心肌炎后遗症3例。显示电镜超微结构的检测对心肌病变的诊断率(40/40)为100％。提示了EMB对VMT诊断的可靠性和敏感性比较高。且证明EMB是一种安全、可靠、简易的方法,对VMT诊断可提供有价值的资料。