全文获取类型
收费全文 | 553篇 |
免费 | 32篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 11篇 |
妇产科学 | 1篇 |
基础医学 | 70篇 |
口腔科学 | 19篇 |
临床医学 | 39篇 |
内科学 | 179篇 |
皮肤病学 | 31篇 |
神经病学 | 31篇 |
特种医学 | 4篇 |
外科学 | 79篇 |
综合类 | 1篇 |
预防医学 | 24篇 |
眼科学 | 3篇 |
药学 | 53篇 |
中国医学 | 4篇 |
肿瘤学 | 35篇 |
出版年
2023年 | 9篇 |
2022年 | 10篇 |
2021年 | 14篇 |
2020年 | 13篇 |
2019年 | 17篇 |
2018年 | 24篇 |
2017年 | 15篇 |
2016年 | 19篇 |
2015年 | 15篇 |
2014年 | 17篇 |
2013年 | 23篇 |
2012年 | 30篇 |
2011年 | 32篇 |
2010年 | 15篇 |
2009年 | 19篇 |
2008年 | 32篇 |
2007年 | 36篇 |
2006年 | 32篇 |
2005年 | 33篇 |
2004年 | 22篇 |
2003年 | 17篇 |
2002年 | 23篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 11篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 1篇 |
1993年 | 5篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1974年 | 1篇 |
1970年 | 2篇 |
排序方式: 共有593条查询结果,搜索用时 23 毫秒
101.
Taguchi N Uemura N Goto Y Sakura M Hara K Niwa M Iida M Yanagishita T Watanabe D Kato M 《Journal of toxicology and environmental health. Part A》2011,74(18):1240-1247
Components of cherry trees have been used as traditional herbal remedies for various diseases. These components are known to possess antioxidative effects. However, the mechanisms underlying cherry tree component-mediated antioxidative effects remain largely unknown. This study focused on cherry leaves extract (CLE) and examined the mechanism underlying the effect of CLE on tert-butyl hydroperoxide (t-BOOH)-induced melanocytic cell death with DNA damage. Interestingly, CLE prevented t-BOOH-induced cell death with reduction in DNA damage, p38 kinase activation, and reactive oxygen species (ROS) production. CLE-mediated suppression of cell death with reduction of DNA damage, p38 kinase activity and ROS production was prevented by a thioredoxin (Trx) system inhibitor but not by a glutathione (GSH) system inhibitor. Finally, data showed that CLE prevented t-BOOH-induced reduction of Trx2 but not Trx1 and Trx reductases (TrxR1 and TrxR2) protein expression. Thus, our results suggest that CLE prevents t-BOOH-induced reduction in Trx2 expression, promotion of ROS production, activation of p38 kinase, and increase in DNA damage and that it protects against cell death. 相似文献
102.
T Moritoyo T Hasunuma K Harada T Tateishi M Watanabe T Kotegawa M Nagai Y Kumagai T Fujitani T Okura T Fukuoka K Miyoshi B Matsuura S Furukawa T Kobori H Moritoyo N Nishikawa T Tsujii H Iwaki M Nakamura S Makino K Ohnuma K Yuji M Hashimoto M Takasu Y Hashizume K You T Matsumura Y Tanaka N Matsumoto J Nakamura J Miura T Akizawa K Kitazawa T Shibata A Kuroki H Honda M Mukai K Ohashi T Morimoto H Imai T Okudaira F Sato J Imanaga K Tanaka M Nomoto 《Journal of pharmacological sciences》2012,119(4):324-329
The effect of renal impairment on the pharmacokinetics of a single oral dose of memantine (10 mg) was determined in Japanese subjects. Subjects were assigned to four groups based on baseline creatinine clearance (CL(CR)): normal renal function (> 80 mL/min, n = 6), and mild (50 to ≤ 80 mL/min, n = 6), moderate (30 to < 50 mL/min, n = 6), and severe renal impairment (5 to < 30 mL/min, n = 7). Mean memantine maximum plasma concentration (C(max)) was similar in the groups (12.66, 17.25, 15.75, and 15.83 ng/mL, respectively), as was mean time to C(max) (6.2, 5.2, 4.3, and 5.4 h, respectively). However, exposure to memantine determined from mean area under the plasma concentration-time curve was 1.62-, 1.97-, and 2.33-times higher in subjects with mild, moderate, and severe renal impairment, respectively, as compared to controls with normal renal function. Mean memantine plasma elimination half-life increased according to increasing renal impairment (61.15, 83.00, 100.13, and 124.31 h, respectively), while mean cumulative urinary recovery of unchanged memantine in 72 h after dosing decreased according to increasing renal impairment (33.68%, 33.47%, 23.60%, and 16.17%, respectively). These results are the same as those in the previous study on caucasian individuals, when compared per body weight. It is suggested that the dose of memantine should be halved in patients with renal impairment. 相似文献
103.
Functional promoter polymorphisms of macrophage migration inhibitory factor in peptic ulcer diseases
Shiroeda H Tahara T Shibata T Nakamura M Yamada H Nomura T Hayashi R Saito T Fukuyama T Otsuka T Yano H Ozaki K Tsuchishima M Tsutsumi M Arisawa T 《International journal of molecular medicine》2010,26(5):707-711
Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator, which plays a pivotal role in inflammatory and immune diseases. We attempted to clarify the association of functional polymorphisms of MIF gene promoter with the development of gastro-duodenal ulcer. The study was performed in 471 stocked DNAs obtained from the subjects, including 93 healthy volunteers, with no evidence of gastric malignancy. We employed the PCR-SSCP method to detect gene polymorphisms. In all 471 DNAs, 92 and 43 were obtained from gastric and duodenal ulcer patients, respectively. By an unadjusted analysis, infection with Helicobacter pylori (H. pylori), male gender and non-steroidal anti-inflammatory drug (NSAID/aspirin) use were significantly associated with a risk for developing a gastric ulcer, whereas MIF promoter polymorphisms were not. On the other hand, infection with H. pylori, male gender and 7-CATT repeat at position -794 were significantly associated with the development of a duodenal ulcer, whereas NSAID/ aspirin use was not. By the analysis after adjustment for age, gender, NSAID/aspirin use and H. pylori infection status, 7/7-CATT homozygote had a significantly increased risk for the development of duodenal ulcers (OR, 6.31; 95% CI, 1.50-26.6; p=0.012). No factors were significantly associated with the development of peptic ulcers in NSAID/aspirin users. Our results suggested that tetranucleotide repeat polymorphism of MIF gene promoter might be associated with the development of duodenal ulcers. 相似文献
104.
Nakamura T Morimoto N Goto F Shioda Y Hoshino H Kubota M Taiji H 《Auris, nasus, larynx》2012,39(6):627-630
Langerhans cell histiocytosis (LCH) is a very rare disease in which granulation tissue forms in various organs and the central nervous system (CNS) due to monoclonal proliferation of Langerhans cells. Some patients develop ataxia, tremor, or neurodegenerative abnormalities (such as personality changes and mental deterioration) several years after the onset as the late effects of LCH. We report a case of a 4-year-old boy with LCH, showing speech disorder, truncal ataxia and a wide-based gait with abnormal findings of central nervous system in CT and MRI image. The results of auditory brain stem response revealed a conduction block in the auditory conduction pathway, suggesting an axonopathy of the brain stem. Disequilibrium may be due to brainstem dysfunction associated with paraneoplastic syndrome because an anti-GluRε2 antibody was seen. Paraneoplastic syndrome is a neuropathy induced through an autoimmune mechanism caused by an antibody directed against the nervous system. Neuro-otological examination is helpful for the assessment of CNS neurodegeneration associated with LCH. 相似文献
105.
Konno T Motoori S Iwamoto N Miyazawa T Saito S Kitagawa N Saisho H Furuse J Itabashi M 《Gan to kagaku ryoho. Cancer & chemotherapy》2010,37(10):1961-1964
A 52-year-old Japanese woman was referred to our Institute because of Helicobacter pylori(H. pylori)-positive gastric mucosa-associated lymphoid tissue(MALT)lymphoma. Since she had a penicillin allergy, we could not eradicate H. pylori using the standard triple therapy including amoxicillin. Additionally, H. pylori was resistant to both clarithromycin and metronidazole. So she was treated with minomycin (MINO), levofloxacin (LVFX), and rabeprazole (RPZ) based on a drug sensitivity test. MINO+LVFX+RPZ appear to be a promising, appropriate, and well-tolerated eradication regimen for H. pylori demonstrating resistance to both clarithromycin and metronidazole, and for patients who are allergic to penicillin. 相似文献
106.
5‐Hydroxyferulic acid methyl ester isolated from wasabi leaves inhibits 3T3‐L1 adipocyte differentiation 下载免费PDF全文
Naoki Misawa Takahiro Hosoya Shuhei Yoshida Osamu Sugimoto Tomoe Yamada‐Kato Shigenori Kumazawa 《Phytotherapy research : PTR》2018,32(7):1304-1310
To investigate the compounds present in wasabi leaves (Wasabia japonica Matsumura) that inhibit the adipocyte differentiation, activity‐guided fractionation was performed on these leaves. 5‐Hydroxyferulic acid methyl ester ( 1 : 5‐HFA ester), one of the phenylpropanoids, was isolated from wasabi leaves as a compound that inhibits the adipocyte differentiation. Compound 1 suppressed the intracellular lipid accumulation of 3T3‐L1 cells without significant cytotoxicity. Gene expression analysis revealed that 1 suppressed the mRNA expression of 2 master regulators of adipocyte differentiation, PPARγ and C/EBPα. Furthermore, 1 downregulated the expression of adipogenesis‐related genes, GLUT4, LPL, SREBP‐1c, ACC, and FAS. Protein expression analysis revealed that 1 suppressed PPARγ protein expression. Moreover, to investigate the relationship between the structure and activity of inhibiting the adipocyte differentiation, we synthesized 12 kinds of phenylpropanoid analog. Comparison of the activity among 1 and its analogs suggested that the compound containing the substructure that possess a common functional group at the ortho position such as a catechol group exhibits the activity of inhibiting the adipocyte differentiation. Taken together, our findings suggest that 1 from wasabi leaves inhibits adipocyte differentiation via the downregulation of PPARγ. 相似文献
107.
Kuwahata M Kuramoto Y Sawai Y Amano S Tomoe Y Segawa H Tatsumi S Ito M Kobayashi Y Kido Y Oka T Miyamoto K 《Journal of nutritional science and vitaminology》2008,54(2):142-147
Our recent study demonstrates that polypyrimidine tract-binding protein (PTB), which is a sequence specific RNA-binding protein, attenuates albumin synthesis in a cell-free translation system. In this study, the effects of food intake on regulation of albumin synthesis through binding of PTB to albumin messenger RNA (mRNA) were investigated. Rats were divided into 1 of 3 groups: fed; fasted for 36 h; or fasted for 36 h and then refed for 24 h. No significant differences in albumin mRNA levels were found among fed, fasted and refed rats. However, a decrease in the proportion of albumin mRNA associated with polysomes was identified in fasted rats. Furthermore, UV-cross linking analysis demonstrated that levels of albumin mRNA-PTB complex were increased in liver extracts from fasted rats. No significant differences in PTB levels in liver homogenate were found among the experimental groups. However, PTB level in the cytoplasmic fraction was higher in fasted rats than in fed rats. In refed rats, PTB level in the cytoplasmic fraction returned to a level comparable to that in fed rats, but was inhibited by treatment with rapamycin, a mammalian target of rapamycin (mTOR) inhibitor. These results suggest that localization of PTB is regulated by food intake through mTOR signaling, and alterations in level of albumin mRNA-PTB complex play a role in mediating the effects of food intake on albumin synthesis in the rat liver. 相似文献
108.
Akiko Narita Yuki Morizane Tomoe Miyake Jiro Seguchi Tetsuya Baba Fumio Shiraga 《Japanese journal of ophthalmology》2017,61(3):253-259
Purpose
To identify the cross-sectional characteristics of filtering blebs associated with intraocular pressure (IOP) control at 1 year after trabeculectomy with a square scleral flap and a fornix-based conjunctival flap.Methods
A retrospective consecutive case series study was conducted. Eighty-four eyes of 79 patients who had undergone primary trabeculectomy were included. Surgical success was defined as IOP ≤15 mmHg and a >20% reduction in IOP without glaucoma medication and without additional glaucoma surgery at 1 year after trabeculectomy. The subjects were classified into two groups according to whether surgery was successful or unsuccessful. Filtering blebs were examined using swept-source three-dimensional anterior segment optical coherence tomography and evaluated for the following quantitative parameters: maximum bleb height, maximum bleb wall thickness, and ratio of hypo-reflective space of the bleb wall.Results
Sixty-six eyes were determined to be successful and 18 eyes unsuccessful. Univariate analysis showed significant differences between the two groups regarding bleb height (P < 0.001), bleb wall thickness (P = 0.011), and ratio of hypo-reflective space of the bleb wall (P = 0.002). Multivariate logistic regression analysis confirmed that the bleb height was significantly associated with success in trabeculectomy [odds ratio 13.996 (95% confidence interval 1.184–165.420); P = 0.036].Conclusion
A tall bleb with a thick hypo-reflective wall may be a feature of the well-functioning bleb.109.
Kosuke Minaga Masayuki Kitano Hajime Imai Kentaro Yamao Ken Kamata Takeshi Miyata Shunsuke Omoto Kumpei Kadosaka Tomoe Yoshikawa Masatoshi Kudo 《World journal of gastroenterology : WJG》2016,22(16):4264-4269
Acute obstructive suppurative cholangitis (AOSC) due to biliary lithiasis is a life-threatening condition that requires urgent biliary decompression. Although endoscopic retrograde cholangiopancreatography (ERCP) with stent placement is the current gold standard for biliary decompression, it can sometimes be difficult because of failed biliary cannulation. In this retrospective case series, we describe three cases of successful biliary drainage with recovery from septic shock after urgent endoscopic ultrasound-guided choledochoduodenostomy (EUS-CDS) was performed for AOSC due to biliary lithiasis. In all three cases, technical success in inserting the stents was achieved and the patients completely recovered from AOSC with sepsis in a few days after EUS-CDS. There were no procedure-related complications. When initial ERCP fails, EUS-CDS can be an effective life-saving endoscopic biliary decompression procedure that shortens the procedure time and prevents post-ERCP pancreatitis, particularly in patients with AOSC-induced sepsis. 相似文献
110.
Nobuyuki Toshikuni Hisakazu Shiroeda Kazuaki Ozaki Yasuhiro Matsue Takahiro Minato Tomoe Nomura Nobuhiko Hayashi Tomiyasu Arisawa Mikihiro Tsutsumi 《Radiology and oncology》2013,47(3):224-229