三七中人参三醇甙对羊心浦氏纤维动作电位及延迟整流钾电流的影响

三七中人参三醇甙(PTS)2.5μg/ml及5.0μg/ml均能明显延长羊心浦氏纤维动作电位时程(APD)包括APD₃₀,APD₅₀,APD₉₀,对动作电位幅度(APA)无明显影响。双微电极法电压钳实验证明,PTS能明显抑制羊心浦氏纤维延迟整流钾电流(I_x)的峰值,且此种抑制作用呈时间及剂量依赖性。揭示PTS通过阻滞延迟整流钾通道而延长APD。     

Vaccine-Induced Th1-Type Responses are Dominant over Th2-Type Responses in the Short Term whereas Pre-existing Th2 Responses are Dominant in the Longer Term

The effect of adjuvant on induction of human papillomavirus type 16 E7 protein-specific cytotoxic T lymphocytes (CTL) and immunoglobulin G (IgG)_2a antibody was studied in C57BL/6 J mice immunized with various adjuvants and E7 protein. Quil-A adjuvant, but not complete Freund's adjuvant (CFA) or Algammulin, induced a T-helper 1 (Th1)-type response to E7, which was characterized by CTL activity against a tumour cell line transfected with E7 protein and by E7-specific IgG_2a. All tested adjuvants elicited comparable levels of E7-specific IgG₁. The longest duration and greatest magnitude of CTL response was seen following two immunizations with the highest dose of E7 and Quil-A. Simultaneous immunization with a Th1 and a T helper 2 (Th2)-promoting adjuvant gave a Th1-type response. However, E7 and Quil-A were unable to induce a Th1-type response (as measured by the inability to generate anti-E7 IgG_2a antibody) in animals with a pre-existing Th2-type response to E7. These results suggest that saponin adjuvants may be suitable for immunotherapy in humans where a Th1-type response is sought, provided that there is no pre-existing Th2-type response to the antigen.     

9－（2－膦酰甲氧乙基）腺嘌呤及其位置异构体3－（2－膦酰甲氧乙基）腺嘌呤的合成和抗病毒活性研究

９－（２－膦酰甲氧乙基）腺嘌呤及其位置异构体３－（２－膦酰甲氧乙基）腺嘌呤的合成和抗病毒活性研究刘晓辉，王琳，贯宝和，孔漫，张兴权，陶佩珍，陈鸿珊（中国医学科学院、中国协和医科大学药物研究所，北京１０００５０；中国医学科学院、中国协和医科大学医药生物...     

Smoking cessation behavior among intermittent smokers versus daily smokers

Nondaily intermittent smokers (ITS) are common, but their cessation behavior remains elusive. We examined cessation of native-ITS (n = 2040), converted-ITS (n = 1808), and daily smokers (DS; n = 25 344). All ITS were more likely than were DS to make a quit attempt (native-ITS adjusted odds ratio [AOR] = 1.60, 95% confidence interval [CI] = 1.42, 1.80; converted-ITS AOR = 3.33, 95% CI = 2.93, 3.78). Native-ITS (18%) and converted-ITS (27%) were more likely than were DS (13%) to quit smoking (native-ITS AOR = 1.34, 95% CI = 1.07, 1.67; converted-ITS AOR = 2.36, 95% CI = 2.01, 2.78), but the low cessation rates of ITS challenge their nonaddicted status.     

Postdischarge smoking cessation in subgroups of hospitalized smokers: A latent class analysis

Background: Hospitalization presents a window of opportunity to treat smoking, and hospital-initiated smoking treatment has demonstrated effectiveness. Despite effective interventions, not all smokers will discontinue use, highlighting the need to better understand which patients achieve cessation. Traditional regression methods may not capture the complexity of inpatient smoker subgroups. Methods: Latent class analysis (LCA) was conducted with data from 397 hospitalized adult cigarette smokers enrolled in a randomized trial. Six categorical indicator variables known to impact cessation were selected to estimate subgroups: health conditions (smoking-related disease [SRD], depressive symptoms, positive screen for alcohol problems) and smoking-related variables (time to first cigarette, cigarettes/day, smoking indoors). The probability of achieving biologically verified 7-day tobacco cessation 6 months after discharged was estimated. Results: A 3-class model best fit the trial data: a Light Smokers subgroup had lower probability for most indicators; a High Health Burden subgroup had high smoking behavior probabilities and similar health problems to the Light Smokers subgroup; and a Heavy Smoking Drinking Depressed subgroup had high nicotine dependence, depressive symptoms, and alcohol misuse probabilities. Probability of biologically verified cessation conditional on class membership was significantly higher (P < .001) for the High Health Burden and the Light Smokers subgroups compared with the Heavy Smoking Drinking Depressed subgroup. Conclusion: Results suggest that subgroups with lower probabilities of alcohol misuse and depression and higher probability of SRD had higher probability of successful cessation after hospital discharge. Hospitalized patients with nicotine dependence combined with behavioral and mental health problems have additional cessation barriers that may require intervention focus.