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51.
Tetsuo Itoh 《Pathology international》1968,18(2):207-225
In allogeneic immunity against tumors induced by methylcholanthrene in the W/KA strain rat, serum antibody agglutinated red blood cells of the W/KA strain rat. The allogeneic antiserum was cytotoxic to the lymphocyte, but not to the tumor cells induced by methylcholanthrene.
In vitro and in vivo studies of allogeneic immunization of tumor suggested that immune macrophages or histiocytes play a major role in the rejection of transplanted tumor. Direct adhesion of immune macrophages with target cells is necessary for destruction of allografted tumor.
In antitransplantation immunity of the syngeneic and autochthonous rat, there was no cytotoxic serum antibody demonstrable in so far as routine techniques were employed. However, this may not exclude the existence of specific serum antibody or non-cytotoxic reaction with target cells of specific serum antibody. On the other hand, immune macrophages were always cytotoxic to target tumor cells, thus showing tumor specifity.
Experiment in which diffusion chambers were inserted into syngeneic immune rats suggested the possible participation of a humoral factor which was released from the immune rats immediately after contact with target cells and transform normal macrophages to immunologically active macrophages. The ascites macrophages have the greatest effective cytotoxic action among the three types of cells. In allogeneic tumor immunity, one immune macrophage can damage one target tumor cell showing single hit interaction. ACTA PATH. JAP. 18: 207–225, 1968. 相似文献
In vitro and in vivo studies of allogeneic immunization of tumor suggested that immune macrophages or histiocytes play a major role in the rejection of transplanted tumor. Direct adhesion of immune macrophages with target cells is necessary for destruction of allografted tumor.
In antitransplantation immunity of the syngeneic and autochthonous rat, there was no cytotoxic serum antibody demonstrable in so far as routine techniques were employed. However, this may not exclude the existence of specific serum antibody or non-cytotoxic reaction with target cells of specific serum antibody. On the other hand, immune macrophages were always cytotoxic to target tumor cells, thus showing tumor specifity.
Experiment in which diffusion chambers were inserted into syngeneic immune rats suggested the possible participation of a humoral factor which was released from the immune rats immediately after contact with target cells and transform normal macrophages to immunologically active macrophages. The ascites macrophages have the greatest effective cytotoxic action among the three types of cells. In allogeneic tumor immunity, one immune macrophage can damage one target tumor cell showing single hit interaction. ACTA PATH. JAP. 18: 207–225, 1968. 相似文献
52.
Tetsuo Ohkuwa Noriaki Fujitsuka Toshikazu Utsuno Miharu Miyamura 《European journal of applied physiology》1980,43(3):235-241
Summary Ventilatory response lines to carbon dioxide at rest were determined by the rebreathing method in 10 untrained subjects, 17 sprint swimmers, and 11 long-distance swimmers. It was found that the mean slope of the ventilatory response line of the swimmer was lower than that of the untrained group, and the mean slope of the long distance swimmer was lower as compared with the sprint swimmer, though these differences were statistically not significant. The differences in the hypercapnic drive between untrained subjects and swimmers obtained here is discussed in connection with their maximum oxygen uptake. 相似文献
53.
Akiko Aoki Shigeru Ono Atsuhisa Ueda Eri Hagiwara Tsuji Takashi Haruko Ideguchi Midori Misumi Tetsuo Sasaki Yoshio Ikezawa Yoshiaki Inayama Yuko Inoue Yoshiaki Ishigatsubo 《Nihon Rinshō Men'eki Gakkai kaishi》2002,25(2):205-211
We described two female patients with primary Sj?gren's syndrome associated with localized cutaneous nodular amyloidosis (LCNA), in which amyloid protein was derived from immunoglobulin light chain. Case 1; a 70-year-old female had complained with polyarthralgia, low-grade fever and parotid gland swelling. She was diagnosed as primary Sj?gren's syndrome. Three years later she noticed brown color small tumor on the thigh and yellow to brown nodules on the bilateral calves of legs. Skin biopsy from the left thigh revealed amyloid L protein deposition, which was positive for anti-lambda light chain staining, in almost entire dermis. Infiltration of lymphocytes and plasma cells around the amyloid deposit were prominent. Case 2; a 51-year-old female had noticed increasing eruption on the hip. Skin biopsy revealed amyloid L protein deposition in the dermis, which was negative for anti-lambda nor kappa light chain staining. When she was refereed to our hospital, she complained of xerostomia and xerophthalmia. She was diagnosed as primary Sj?gren's syndrome. In both cases, histological examination of a minor salivary gland biopsy revealed infiltration of lymphocytes and plasma cells but not amyloid deposit. Serum M protein and urine Bence-Jones protein were not detected. These cases represent localized amyloidosis without systemic involvement. It is widely recognized that Sj?gren's syndrome is frequently accompanied by B cell lymphoproliferative disorders. In LCNA, infiltration of plasma cells around the amyloid deposits was frequently prominent. The relation between these two disorders is discussed. 相似文献
54.
Persistent effect of a low dose of preadministered diethylnitrosamine on the induction of enzyme-altered foci in rat liver 总被引:1,自引:0,他引:1
Takahashi Seiichi; Tsutsumi Masahiro; Nakae Dai; Denda Ayumi; Kinugasa Tetsuo; Konishi Yoichi 《Carcinogenesis》1987,8(4):509-513
The effect of a low dose of preadministered diethylnitrosamine(DEN) on the induction of enzyme-altered foci in the liversof male full-grown Fischer 344 rats was studied. As a pretreatment,DEN at a dose of 10 mg/kg body wt was injected i.p. At varioustimes after DEN pretreatment a complete initiation, consistingof administration of the same dose of DEN by the same routein rats subjected to partial hepatectomy (PH), was performed,followed by application of selection pressure. Enzyme-alteredfoci stained with -glutamyltrans-peptidase (-GTP) and glutathioneS-transferase placental form (GST-P) were then assayed. Decreasesin the numbers and areas of foci in the rats which receivedsaline + PH 14 or 28 days after DEN pretreatment were observedin comparison with rats which received saline + PH immediatelyafter DEN. On the other hand, the numbers and areas of fociwere not decreased in rats which received the complete initiation,consisting of DEN + PH, at various times after DEN pretreatmentwhen compared with rats which received these at the same timeas the DEN pretreatment. This persistent effect of DEN pretreatmenton the complete initiation lasted up to 182 days after the timeof DEN pretreatment. In this experiment, GST-P was found tobe a more sensitive marker for the detection of putative preneoplasticliver-cell foci than -GTP. 相似文献
55.
Shinichi Izuchi Tohru Terachi Masahiro Sakamoto Tetsuo Mikami Mamoru Sugita 《Current genetics》1990,18(3):239-243
Summary The nucleotide sequences of tRNAAsn (GUU) and tRNATyr (GUA) genes from tomato mitochondria and their flanking regions have been determined. The tomato mitochondrial tRNAAsn gene is located 2.1 kb downstream from the tRNACys gene reported previously (Izuchi and Sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. The tRNATyr gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a native mitochondrial tRNA gene and is 535 bp from the chloroplast-like tRNAAsn gene on the same strand. Northern hybridization analysis revealed that the three tRNA genes are transcribed in tomato mitochondria. Southern hybridization analysis of tomato, sugar beet, rice and wheat mitochondrial DNAs, with oligonucleotide probes for mitochondrial or chloroplast tRNA genes, demonstrated that the mitochondrial tRNACys gene found in tomato is present in dicot plants but not in monocots. On the other hand, a chloroplast-like tRNACys gene exists in monocot plants. 相似文献
56.
Mitsuo Shimada Katsuhiko Yanaga Keiji Kishikawa Saburo Kakizoe Hidetoshi Itasaka Tetsuo Ikeda Taketoshi Suehiro Keizo Sugimachi 《Transplant international》1993,6(1):4-7
Rapid and reliable assessment of hepatic graft viability is important for successful orthotopic liver transplantation (OLTx). OLTx was performed in 11 pairs of pigs via a venovenous bypass. Six of these grafts were transplanted immediately (group A), while the other five were preserved in University of Wisconsin (UW) solution for 24 h and then transplanted (group B). All grafts were flushed with 300 ml of chilled (4°C) Ringer's lactate solution before reperfusion of the graft, when 20 ml of effluent from the graft was collected and the concentrations of ammonia, lactic acid, GOT, and LDH were measured. Four of the six pigs in group A survived longer than 3 days, while the other two pigs died of causes other than graft dysfunction. All five pigs in group B died either of hemoperitoneum or hemodynamic instability due to liver failure. The histology of postperfusion biopsies in group A showed minimal pathological changes, while the grafts in group B revealed moderate to severe ischemic injuries. Ammonia and lactic acid in the effluent of group B were significantly higher than those of group A (1511±216 vs 417±333 g/dl and 114.1±12.2 vs 91.4±12.2 mg/dl, respectively; P<0.05 in both cases). Before reperfusion, the rate of total adenine nucleotides in all of the substances in the graft, which were measured using high performance liquid chromatography (HPLC), inversely correlated with the ammonia levels in the effluent. We conclude that an analysis of the effluent, (i.e. the levels of ammonia and lactic acid), flushed from a hepatic graft before reperfusion could serve as a predictor of hepatic graft viability. 相似文献
57.
58.
The effects of butorphanol injection on baroreflex control of heart rate were investigated using both pressor and depressor tests in eighteen adult patients. Baroreflex sensistivity was attenuated after butorphanol injection in the pressor test using phenylephrine, whereas it was unchanged in the depressor test using nitroglycerine. No resetting of the baroreflex occurred after butorphanol injection. After the administration of butorphanol, plasma epinephrine and norepinephrine levels increased. These results suggest that it is safe to use butorphanol clinically even when a reduction in blood pressure due to hypovolemia or unclamping of the major artery is expected and that it is disadvantageous to administer the drug when an increase in blood pressure due to cross-clamping of the major artery is predicted.(Wajima Z, Inoue T and Ogawa R: The effects of butorphanol on baroreflex control of heart rate in man. J Anesth 7: 411--418, 1993) 相似文献
59.
Terashima Keisuke Takai Satomi Usami Yoshiko Adachi Tetsuo Sugiyama Tadashi Katagiri Yoshihiro Hirano Kazuyuki 《Pharmaceutical research》1996,13(9):1327-1330
Purpose. Indomethacin is well known to be metabolized via O-demethylation and N-deacylation. In this paper we found an enzyme involved in the hydrolysis of amide-linkage of indomethacin and partially characterized it as well as its substrate specificity.
Methods. An indomethacin hydrolyzing enzyme was purified to homogeneity from pig liver microsomes using columns of Q-Sepharose, Red-Sepharose and Blue-Sepharose. The enzyme activity was assayed by measuring of -chlorobenzoic acid liberated from indomethacin by HPLC.
Results. The purified enzyme effectively hydrolyzed the amide linkage in indomethacin but not those in -naphthylacetate and -nitrophenylacetate, which are typical substrates for carboxylesterase. The subunit molecular mass of the enzyme was 65 kDa according SDS-polyacrylamide gel electrophoresis. The Michaelis constant (Km) and maximum velocity (Vmax) values for indomethacin were 67.8 µM and 9.02 nmol/min/mg protein, respectively. The amino acid sequence analysis of the enzyme after cyanogen bromide cleavage showed high homology with a mouse carboxylesterase isozyme designated as ES-male. The activity of indomethacin hydrolysis was relatively high in the pig, rabbit and human liver homogenate, but not in those from rat and mouse. On the other hand, purified human liver carboxylesterases pl 5.3 and 4.5, and pig liver carboxylesterases have no catalytic activity for indomethacin.
Conclusions. These results indicate that the hydrolysis of amide-linkage of indomethacin in humans would be associated with an enzyme similar to the indomethacin hydrolyzing enzyme from pig liver microsomes described here. 相似文献
60.
Tohru Ide Tetsuo Kochi Kazuhiko Iijima Tadanobu Mizuguchi 《Journal canadien d'anesthésie》1996,43(1):44-49