全文获取类型
收费全文 | 2149篇 |
免费 | 130篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 19篇 |
儿科学 | 56篇 |
妇产科学 | 32篇 |
基础医学 | 309篇 |
口腔科学 | 64篇 |
临床医学 | 162篇 |
内科学 | 533篇 |
皮肤病学 | 45篇 |
神经病学 | 146篇 |
特种医学 | 79篇 |
外科学 | 188篇 |
综合类 | 7篇 |
一般理论 | 1篇 |
预防医学 | 117篇 |
眼科学 | 54篇 |
药学 | 235篇 |
中国医学 | 2篇 |
肿瘤学 | 239篇 |
出版年
2023年 | 17篇 |
2022年 | 35篇 |
2021年 | 69篇 |
2020年 | 38篇 |
2019年 | 45篇 |
2018年 | 53篇 |
2017年 | 32篇 |
2016年 | 34篇 |
2015年 | 70篇 |
2014年 | 62篇 |
2013年 | 92篇 |
2012年 | 170篇 |
2011年 | 153篇 |
2010年 | 91篇 |
2009年 | 73篇 |
2008年 | 148篇 |
2007年 | 175篇 |
2006年 | 170篇 |
2005年 | 142篇 |
2004年 | 132篇 |
2003年 | 133篇 |
2002年 | 100篇 |
2001年 | 22篇 |
2000年 | 15篇 |
1999年 | 26篇 |
1998年 | 29篇 |
1997年 | 16篇 |
1996年 | 17篇 |
1995年 | 18篇 |
1994年 | 17篇 |
1993年 | 14篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有2288条查询结果,搜索用时 15 毫秒
31.
Kazutoshi Higuchi Seiji Futagami Hiroshi Yamawaki Makoto Murakami Kumiko Kirita Shuhei Agawa Go Ikeda Hiroto Noda Yasuhiro Kodaka Nobue Ueki Keiko Kaneko Katya Gudis Ryuji Ohashi Katsuhiko Iwakiri 《Journal of Clinical Biochemistry and Nutrition》2021,68(1):86
Since the prevention of early chronic pancreatitis (ECP) into chronic pancreatitis might be critical for the reduction of pancreatic cancer, we tried to clarify the pathophysiology of ECP patients, focusing on ECP patients without alcoholic chronic pancreatitis. 27 ECP patients without alcoholic chronic pancreatitis and 33 patients with functional dyspepsia with pancreatic enzyme abnormalities (FD-P) were enrolled in this study. Diagnosis of ECP was made when imaging findings showed the presence of more than 2 out of 7 endoscopic ultrasound features. Duodenal degranulated eosinophils and glucagon-like peptide 1 producing cells were estimated by immunostaining. There were no significant differences in characteristics and psychogenic factors between ECP and FD-P patients. Interestingly, endoscopic ultrasound score in ECP patients significantly improved, albeit clinical symptoms in ECP patients showed no improvement at one year follow up. The extent of migration of duodenal degranulated eosinophils in FD-P patients was significantly higher compared to that in ECP patients. The levels of elastase-1 and trypsin in ECP patients with improved endoscopic ultrasound features were significantly reduced by the treatment. Further studies will be needed to clarify whether clinical symptoms and endoscopic ultrasound features in ECP patients without alcoholic chronic pancreatitis were improved in longer follow up study. 相似文献
32.
Shohei Nomura Mitsuru Kashiwagi Takuya Tanabe Chizu Oba Kumiko Yanagi Tadashi Kaname Nobuhiko Okamoto Akira Ashida 《Brain & development》2021,43(4):566-570
BackgroundRapid-onset dystonia–parkinsonism (RDP) is a disease characterized by an abrupt onset of dystonia accompanied by signs of parkinsonism and prominent bulbar symptoms.Case reportWe describe a case of a female patient, born after normal delivery, but diagnosed with mild intellectual disability at age 7. She presented with an abrupt onset of upper limb dystonia and bradykinesia without tremor in parkinsonism, as well as dysarthria and dysphagia caused by prominent bulbar symptoms, at age 9. She had normal findings on brain magnetic resonance imaging, electroencephalography, and blood examination but was diagnosed with a psychogenic disorder. At age 10, she developed left lower limb paroxysmal stiffness with pain, and at 14, she was hospitalized due to lasting paroxysmal symptoms. Whole-exome sequencing was performed for this index case and her parents, and a de novo missense variant c.829G > A, p.Glu277Lys in ATP1A3 was identified.DiscussionThis RDP case highlights a rare clinical feature of paroxysmal dystonia that affects the lower left limb and develops after the abrupt onset of permanent dystonia. Currently, there are only three reported RDP cases associated with the same missense mutation, and we summarized the clinical features of all cases including ours, such as onset of age, time for stable, RDP score, relapse and exacerbation. Various symptoms owing to ATP1A3 mutation could develop as ATP1A3-related neurological disorders beyond classical phenotypes such as alternating hemiplegia of childhood (AHC) or RDP. Although RDP is extremely rare during childhood, it is important to understand its clinical characteristics in children. 相似文献
33.
34.
35.
36.
Shokoji T Fujisawa Y Kimura S Rahman M Kiyomoto H Matsubara K Moriwaki K Aki Y Miyatake A Kohno M Abe Y Nishiyama A 《Hypertension》2004,44(2):236-243
We have recently shown that systemic administration of a superoxide dismutase mimetic, tempol, resulted in decreases in mean arterial pressure and heart rate along with a reduction in renal sympathetic nerve activity (RSNA). It has also been shown that these parameters are significantly increased by systemic administration of a superoxide dismutase inhibitor, diethyldithio-carbamic (DETC), indicating a potential role of reactive oxygen species in the regulation of RSNA. In this study, we examined the effects of local administrations of 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol) and DETC on RSNA in anesthetized rats. Either tempol or DETC was directly administered onto the renal sympathetic nerves located between the electrode and ganglion. Local application of tempol (10 microL, 0.17 to 1.7 mol/L, n=6) resulted in dose-dependent decreases in integrated RSNA (by -81+/-6% at 1.7 mol/L) without alterations in mean arterial pressure and heart rate. In contrast, DETC (10 microL, 0.17 to 1.7 mol/L, n=6) increased RSNA dose-dependently. The responses of RSNA to tempol and DETC were significantly greater in spontaneously hypertensive rats than in normotensive rats (n=6, respectively). Local application of sodium nitroprusside (1 mmol/L) or N(G)-nitro-L-arginine methyl ester (0.11 mol/L) altered neither basal RSNA nor tempol-induced reductions in RSNA (n=6 and 5, respectively). A voltage-gated potassium channel blocker, 4-aminopyridine (0.1 mol/L), significantly decreased basal RSNA (by -81+/-1%) and completely prevented DETC-induced increases in RSNA (n=5). These results suggest that reactive oxygen species play a role in the regulation of peripheral sympathetic nerve activity, and that at least part of this mechanism is mediated through voltage-gated potassium channels. 相似文献
37.
Identification of simultaneous mutation of fibrinogen alpha chain and protein C genes in a Japanese kindred 总被引:2,自引:0,他引:2
Watanabe K Shibuya A Ishii E Kurihara M Inoue S Ono M Wada Y Wakiyama M Zaitsu M Iida H Muraoka K Kinoshita S Hamasaki N 《British journal of haematology》2003,120(1):101-108
Afibrinogenaemia usually induces a bleeding tendency during infancy, whereas protein C deficiency increases susceptibility to thrombosis in children or adolescence. Mutations of these genes have been, therefore, established as independent risk factors for coagulation disorders. We describe the homozygous mutation of the fibrinogen alpha chain gene and additional heterozygous mutation of the protein C gene in a male infant who showed prolonged umbilical bleeding after birth. On examination, the plasma fibrinogen was undetectable, and the activity and antigen level of protein C were reduced. The patient showed no fibrinogen Aalpha chain as well as Bbeta and gamma chains by Western blotting. The sequencing analysis showed the homozygous deletion of 1238 bases from intron 3 at position 2008 to intron 4 at position 3245 in the fibrinogen alpha chain gene. Both parents were heterozygous carriers of this mutation. In this patient, an additional mutation was also detected in the protein C gene: the heterozygous deletion of exon 7 at position 6161-6163 or 6164-6166, resulting the deletion of one amino acid (Lys150 or 151). His mother was also a carrier of this mutation. As the simultaneous mutation of the fibrinogen alpha chain and protein C genes has not been previously reported, the influence of the interaction between these two mutations on the clinical manifestations of this patient should be carefully monitored for a long period. 相似文献
38.
Ian E. Burbulis Kumiko Yamaguchi Olga V. Nikolskaia Sean T. Prigge Stefan Magez Sylvie Bisser Megan E. Reller Dennis J. Grab 《Clinical and Vaccine Immunology : CVI》2015,22(4):374-380
Loop-mediated isothermal amplification (LAMP) is a method for enzymatically replicating DNA that has great utility for clinical diagnosis at the point of care (POC), given its high sensitivity, specificity, speed, and technical requirements (isothermal conditions). Here, we adapted LAMP for measuring protein analytes by creating a protein-DNA fusion (referred to here as a “LAMPole”) that attaches oligonucleotides (LAMP templates) to IgG antibodies. This fusion consists of a DNA element covalently bonded to an IgG-binding polypeptide (protein L/G domain). In our platform, LAMP is expected to provide the most suitable means for amplifying LAMPoles for clinical diagnosis at the POC, while quantitative PCR is more suitable for laboratory-based quantification of antigen-specific IgG abundance. As proof of concept, we measured serological responses to a protozoan parasite by quantifying changes in solution turbidity in real time. We observed a >6-log fold difference in signal between sera from vaccinated versus control mice and in a clinical patient sample versus a control. We assert that LAMPoles will be useful for increasing the sensitivity of measuring proteins, whether it be in a clinical laboratory or in a field setting, thereby improving acute diagnosis of a variety of infections. 相似文献
39.