全文获取类型
收费全文 | 36266篇 |
免费 | 1728篇 |
国内免费 | 297篇 |
专业分类
耳鼻咽喉 | 271篇 |
儿科学 | 666篇 |
妇产科学 | 415篇 |
基础医学 | 4825篇 |
口腔科学 | 1011篇 |
临床医学 | 2115篇 |
内科学 | 9024篇 |
皮肤病学 | 860篇 |
神经病学 | 2519篇 |
特种医学 | 1272篇 |
外科学 | 6522篇 |
综合类 | 140篇 |
一般理论 | 1篇 |
预防医学 | 984篇 |
眼科学 | 632篇 |
药学 | 2130篇 |
中国医学 | 125篇 |
肿瘤学 | 4779篇 |
出版年
2023年 | 183篇 |
2022年 | 414篇 |
2021年 | 891篇 |
2020年 | 456篇 |
2019年 | 652篇 |
2018年 | 861篇 |
2017年 | 660篇 |
2016年 | 781篇 |
2015年 | 805篇 |
2014年 | 1082篇 |
2013年 | 1386篇 |
2012年 | 2262篇 |
2011年 | 2635篇 |
2010年 | 1490篇 |
2009年 | 1230篇 |
2008年 | 2256篇 |
2007年 | 2487篇 |
2006年 | 2329篇 |
2005年 | 2508篇 |
2004年 | 2424篇 |
2003年 | 2406篇 |
2002年 | 2415篇 |
2001年 | 370篇 |
2000年 | 318篇 |
1999年 | 442篇 |
1998年 | 577篇 |
1997年 | 459篇 |
1996年 | 432篇 |
1995年 | 371篇 |
1994年 | 323篇 |
1993年 | 302篇 |
1992年 | 216篇 |
1991年 | 201篇 |
1990年 | 203篇 |
1989年 | 170篇 |
1988年 | 154篇 |
1987年 | 116篇 |
1986年 | 111篇 |
1985年 | 100篇 |
1984年 | 108篇 |
1983年 | 88篇 |
1982年 | 74篇 |
1981年 | 88篇 |
1980年 | 83篇 |
1979年 | 60篇 |
1978年 | 41篇 |
1977年 | 30篇 |
1976年 | 24篇 |
1975年 | 23篇 |
1973年 | 24篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
Functional engraftment of human peripheral T and B cells and sustained production of autoantibodies in NOD/LtSzscid/IL‐2Rγ−/− mice
下载免费PDF全文
![点击此处可从《European journal of immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yuki Ishikawa Takashi Usui Aoi Shiomi Masakazu Shimizu Kosaku Murakami Tsuneyo Mimori 《European journal of immunology》2014,44(11):3453-3463
NOD/LtSzscid/IL‐2Rγ?/? (NSG) mice have advantages in establishing humanized mouse models. However, transferring human PBMCs into these mice often causes lethal GVH disease. In this study, we discovered an improved method for the engraftment of normal or pathological human PBMCs into NSG mice and examined the subsequent induction of specific immune responses. We sequentially transferred human CD4+ memory T (Tm) and B cells obtained from PBMCs of healthy adults or patients with autoimmune diseases into NSG mice. Removing naïve CD4+ T cells from the transferred PBMCs allowed successful engraftment without lethal GVH disease. The transferred Tm cells were found to reside mainly in the spleen and the lymphoid nodules, where they expressed MHC class II molecules and produced cytokines, including IL‐21. Surprisingly, the transferred B cells were also well maintained in the lymphoid organs, underwent de novo class‐switch recombination, and secreted all isotypes of human Igs at significant levels. Moreover, transferring patient‐derived Tm and B cells resulted in sustained production of IgM‐rheumatoid factor and antiaminoacyl transfer RNA synthetase Abs in these mice. These results suggest that transfer of Tm and B cells derived from human PBMCs into NSG mice could be a useful method for the study of human autoimmune mechanisms. 相似文献
993.
Cheng-Tse Wu Ahmed I. A. Soliman Toru Utsunomiya Takashi Ichii Hiroyuki Sugimura 《RSC advances》2019,9(55):32313
Formation of precise and high-resolution silica micropatterns on polymer substrates is of importance in surface structuring for flexible device fabrication of optics, microelectronic, and biotechnology. To achieve that, substrates modified with affinity-patterns serve as a strategy for site-selective deposition. In the present paper, vacuum ultraviolet (VUV) treatment is utilized to achieve spatially-controlled surface functionalization on a cyclo-olefin polymer (COP) substrate. An organosilane, 2,4,6,8-tetramethylcyclotetrasiloxane (TMCTS), preferentially deposits on the functionalized regions. Well-defined patterns of TMCTS are formed with a minimum feature of ∼500 nm. The secondary VUV/(O)-treatment converts TMCTS into SiOx, meanwhile etches the bare COP surface, forming patterned SiOx/COP microstructures with an average height of ∼150 nm. The resulting SiOx patterns retain a good copy of TMCTS patterns, which are also consistent with the patterns of photomask used in polymer affinity-patterning. The high quality SiOx patterns are of interests in microdevice fabrication, and the hydrophilicity contrast and adjustable heights reveal their potential application as a “stamp” for microcontact printing (μCP) techniques.Patterned surface treatment on a polymer substrate is carried out by 172 nm VUV through a photomask. TMCTS pattern formation is guided by the resulting affinity-pattern. The secondary VUV treatment converted TMCTS patterns into silica patterns. 相似文献
994.
995.
996.
997.
Saneyuki Takaichi Takashi Muramatsu Jong-Min Lee Han-Sung Jung Naoshi Shinozaki Akira Katakura Gen-yuki Yamane 《ACTA HISTOCHEMICA ET CYTOCHEMICA》2014,47(5):195-201
Alkaline conditions in the oral cavity may be caused by a variety of stimuli, including tobacco products, antacids, alkaline drinking water and bicarbonate toothpaste. However, the effects of an alkaline pH on the oral mucosa had not been elucidated. The purpose of this study was to investigate how basal keratinocytes are actively involved in re-epithelialization after alkaline chemical injury. We generated epithelial defects in the oral mucosa of mice by applying an alkaline chemical, and the localization of cytokeratin 13, cytokeratin 14, PCNA and p63 was investigated during the re-epithelialization process. PCNA- and p63-positive staining was seen in basal cells covering the wound surface at 1 day after the chemical injury. Cytokeratin 14-positive and PCNA-negative basal keratinocytes were localized in a few layers of the wound epithelium during epithelial outgrowth. Cytokeratin 14-positive and PCNA-positive basal keratinocytes, indicating proliferation, were localized over the entire layer of the epithelium at the wound margin. These results imply that basal keratinocytes at the wound margin migrate to the wound surface, provoke differentiation and keratinization during epithelial outgrowth and that epithelial cells are supplied from the wound margin to the epithelial outgrowth after alkaline chemical injury. 相似文献
998.
999.
1000.