全文获取类型
收费全文 | 453篇 |
免费 | 8篇 |
专业分类
儿科学 | 57篇 |
妇产科学 | 1篇 |
基础医学 | 26篇 |
口腔科学 | 7篇 |
临床医学 | 59篇 |
内科学 | 108篇 |
皮肤病学 | 12篇 |
神经病学 | 37篇 |
外科学 | 76篇 |
综合类 | 3篇 |
预防医学 | 5篇 |
药学 | 38篇 |
肿瘤学 | 32篇 |
出版年
2016年 | 3篇 |
2015年 | 7篇 |
2014年 | 2篇 |
2013年 | 10篇 |
2012年 | 7篇 |
2011年 | 3篇 |
2010年 | 13篇 |
2009年 | 10篇 |
2008年 | 5篇 |
2007年 | 8篇 |
2006年 | 37篇 |
2005年 | 14篇 |
2004年 | 13篇 |
2003年 | 14篇 |
2002年 | 9篇 |
2001年 | 7篇 |
2000年 | 3篇 |
1999年 | 11篇 |
1998年 | 34篇 |
1997年 | 31篇 |
1996年 | 40篇 |
1995年 | 43篇 |
1994年 | 22篇 |
1993年 | 13篇 |
1992年 | 13篇 |
1991年 | 8篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1986年 | 7篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1957年 | 2篇 |
1956年 | 3篇 |
1955年 | 5篇 |
1954年 | 4篇 |
排序方式: 共有461条查询结果,搜索用时 15 毫秒
101.
102.
RYO MURAMATSU YASUHIKO KOMATSU ERIKO NUKUI TORU OKAYAMA TADANORI MORIKAWA KYOICHI KOBASHI HIDEYA HAYASHI 《Chemical biology & drug design》1996,48(2):167-173
To clarify the role of the negative charge of the C-terminal region of hirudin, we chemically synthesized the C-terminal peptide of hirudin variant-1 (HV-1), HV-1-(54-65), and its analogs, [E61Y,E62Y]HV-1-(54-65) and [E62Y]HV-1-(54-65), and then sulfated the Tyr residue(s) in these peptides by both enzymic and chemical methods. Enzymic O-sulfation of Tyr residues in the peptides by use of sulfotransferase isolated from Eubacterium A-44 allowed us to produce four kinds of the sulfated peptide, whose C-terminal sequences were -PEY(SO3H)YLQ, -PYY(SO3H)YLQ, -PYYY(SO3H)LQ and -PYY(SO3H)Y(SO3H)LQ. On the other hand, all Tyr residues in the peptides were successfully sulfated by chemical reaction with N, N′-dicyclohexylcarbodiimide in the presence of sulfuric acid. Based on the analysis of structure-activity relationships of these sulfated peptides for thrombin inhibition, the Tyr62 and Tyr63 bisulfated peptide GDFEEIPEY(SO3H)Y(SO3H)LQ was found to be the most potent inhibitor of thrombin among the products tested. No increase in potency was observed by further substitution of Glu61 with Tyr(SO3H). The inhibitory activity by substitution with Tyr(SO3H) at position 63 was greater than that obtained by the substitution at position 62. © Munksgaard 1996. 相似文献
103.
Effects of genetic defects in the CYP2C19 gene on the N-demethylation of imipramine, and clinical outcome of imipramine therapy 总被引:1,自引:0,他引:1
SHIGERU MORINOBU md TAKESHI TANAKA md SHINOBU KAWAKATSU md SHIRO TOTSUKA md ERIKO KOYAMA p hd KAN CHIBA p hd TAKASHI ISHIZAKI md TAKAHIRO KUBOTA ms 《Psychiatry and clinical neurosciences》1997,51(4):253-257
Abstract The relationship between the genetic polymorphism of S-mephenytoin 4'-hydroxylation catalyzed by CYP2C19 and the N-demethylation of imipramine was examined in 10 Japanese depressed patients. Five patients, who were poor metabolizers of S-mephenytoin, were determined to be either homozygous for a mutation in exon 5 or heterozygous for mutations in exon 4 and exon 5 of the CYP2C19 gene. In contrast, five patients, who were extensive metabolizers, had no mutations. The demethylation index (the desipramine/imipramine ratio) was significantly lower in patients with genetic defects. Plasma levels of imipramine and 2-hydroxyimipramine normalized by the daily dose (mg) per weight (kg) were significantly higher in patients with genetic defects. This suggests that the N-demethylation of imipramine is impaired in patients with genetic defects in the CYP2C19 gene, and that genotype determination may be useful in preventing side effects induced by unexpectedly elevated levels of imipramine. 相似文献
104.
YASUSUKE KIMOTO SADAAKI SAKAMOTO KEITA FUJIKAWA TAKASHI TACHIBANA NORIYUKI YAMAMOTO TOSHIKAZU OTANI 《International journal of urology》2006,13(11):1428-1433
AIM: Vardenafil is a highly selective phosphodiesterase type-5 inhibitor for the treatment of erectile dysfunction (ED). Efficacy of vardenafil has been demonstrated in various ED populations, but that in Japanese patients with spinal cord injury (SCI) has not been assessed. METHODS: This was an open-label, multicenter, flexible dose, 12-week study in patients with ED due to SCI. Following a 4-week observation period, patients received vardenafil 10 mg for 4 weeks, and based on efficacy, tolerability and patient preference, doses for the remaining 8 weeks were decided by investigators. The primary efficacy parameter was erectile function domain score of the International Index of Erectile Function. RESULTS: Ten patients took 10 mg all through the study, while 22 patients took 20 mg after completing 4 weeks' treatment with 10 mg. The erectile function domain score increased from 12.2 at baseline to 25.0 at Last Observation Carried Forward (LOCF) in the former group and from 10.3 to 22.5 in the latter group, respectively. Importantly, there was a 5.0 point increase in erectile function domain score after up-titration in the latter group. Drug-related adverse events were observed in 22% of patients including hot flushes (9%) and headache (6%), but these were transient and mild in intensity. Serious adverse events and adverse events leading to discontinuation of the study drug were not reported. CONCLUSIONS: Vardenafil 10 and 20 mg was well tolerated and improved erectile function in patients with SCI. Of interest, erectile function was further improved by 20 mg in patients who were not sufficiently treated with 10 mg. 相似文献
105.
SAORI NISHIJIMA KIMIO SUGAYA TAKASHI FUKUDA MINORU MIYAZATO SATOSHI ASHIMINE YOSHIHIDE OGAWA 《International journal of urology》2006,13(12):1479-1483
OBJECTIVE: Our previous study showed that the spinal glycine level in rats was changed by spinal injury or bladder outlet obstruction, and this change was reflected by serum glycine levels. Therefore, we measured the serum glutamate and glycine levels in healthy volunteers and patients with cerebrospinal damage or benign prostatic hyperplasia (BPH) to confirm whether the change of serum amino acid levels was obtained from these patients as well as the animal experiment. METHODS: We measured the serum glutamate and glycine levels in 170 healthy controls, 57 patients with cerebrovascular disease (CVD), 68 patients with spinal cord injury (SCI), and 70 patients with BPH. Amino acid levels were compared between the controls and patients, according to gender, level of spinal injury and the type of bladder activity. RESULTS: In the healthy controls, glutamate levels were higher and glycine levels were lower in men than in women. On group comparison of each gender, there were no differences of glutamate levels. However, glycine levels were lower in male and female SCI patients and BPH patients than in controls. According to the level of spinal injury or the pattern of bladder activity and amino acid levels, there were no relationships among them. CONCLUSIONS: Serum glutamate and glycine levels were not related to the spinal injury level or bladder activity. However, serum glycine levels changed in patients with SCI or BPH patients, so it may be possible to use it as an indicator of spinal glycinergic neuronal activity. 相似文献
106.
Targeted Disruption of the IL-6 Related Genes: gp130 and NF-IL-6 总被引:5,自引:0,他引:5
SHIZUO AKIRA KANJI YOSHIDA TAKASHI TANAKA TETSUYA TAGA TADAMITSU KISHIMOTO 《Immunological reviews》1995,148(1):221-253
107.
TAKASHI KOBAYASHI KEIJI OGURA KOJI NISHIZAWA MAKOTO ONO YASUSHI UENO HIDENORI MIYAKE YOSHIHIRO IDE 《International journal of urology》2004,11(1):47-49
A 70-year-old man who had undergone radical surgery for renal pelvic transitional cell carcinoma 9 months previously developed solitary cerebellar metastasis. Despite neurosurgical removal, the patient died and post-mortem pathological examination revealed microscopic metastatic lesions within microvessels of the lung. No other lesion, including local residual cancer, was detected. 相似文献
108.
Metanephric Adenoma Treated with Laparoscopic Nephrectomy 总被引:2,自引:0,他引:2
TAKASHI EBINE REI OHARA TETSUO MOMMA SHIRO SAITO SHIGERU KURAMOCHI 《International journal of urology》2004,11(4):232-234
A 31-year-old-female presented with a left renal mass detected incidentally during an abdominal ultrasound examination. Computed tomography and angiography revealed a hypovascular solid tumor 4.5 cm in diameter at the middle portion of the left kidney. Laparoscopic left nephrectomy was performed and macroscopic examination of the specimen revealed encapsulated tumor with grayish yellow cut surface. Histological examination demonstrated that the tumor cells were small scant cytoplasms, containing round and regular nuclei, forming a glomerular-like structure. There was no mitosis nor cellular atypia indicating a malignant structure. The histological diagnosis was metanephric adenoma. 相似文献
109.
RYOTA HASHIMOTO md phd SATOKO HATTORI p hd SACHIE CHIBA ms c YUKI YAGASAKI ms c TAKEYA OKADA p hd EMI KUMAMARU p hd TAKEYUKI MORI md KIYOTAKA NEMOTO md HISASHI TANII md phd HIROAKI HORI md HIROKO NOGUCHI ma TADAHIRO NUMAKAWA p hd TAKASHI OHNISHI md phd HIROSHI KUNUGI md phd 《Psychiatry and clinical neurosciences》2006,60(S1):S4-S10
Abstract It is well known that genetic factors contribute to the susceptibility for schizophrenia. Recent advances in the molecular genetics of schizophrenia strongly suggest several susceptibility genes (e.g. dysbindin, neuregulin-1, DISC1, COMT, G72, RGS4 and Akt1). We discuss the evidence and biology of these genes. As glutamate transmission is especially implicated in these genes, neurobiological basis of schizophrenia might be elucidated by investigation of functional interactions between susceptibility genes for schizophrenia and the glutamatergic system. 相似文献
110.
ILL YOUNG SEO YOSHINARI ONO YOKO YOSHIKAWA TAKASHI SAIKA YASUSHI YOSHINO SATOSHI KATSUNO HIDEMORI ARAKI SHINICHI OHSHIMA 《International journal of urology》2004,11(9):778-781
We report our experience with laparoscopic radical nephrectomy for a 79-year-old man who had renal cell carcinoma (RCC) with a renal vein thrombus. For the transaction of the renal vein with the thrombus, we used an endoscopic gastrointestinal anastomosis stapler. The operating time was 4 h and blood loss was 400 mL. The patient could walk and drink on the first postoperative day. He recovered normal activity 30 days postoperatively. There were no intraoperative and postoperative complications. The present report demonstrates the feasibility of laparoscopic radical nephrectomy in patients with T3b RCC who suffer from tumor thrombus in the renal vein. 相似文献