The mechanisms of central regulation disorder under combat exposure conditions

Results of research in central regulation system's disorders in the local wars' participants suffering from environmental psychoemotional and traumatic stress. It has been established, that there was an upsetting of interaction between neurodynamic processes in the sub-dominant hemisphere and a reduction of the functional reserve of the brain. Neurophysiology disorders discovered come from the pathological system that had been formed up in the central nervous system preserve their persistence for several years after the war.     

Mutations in the erythropoietin receptor gene are not a common cause of Diamond-Blackfan anemia

Diamond-Blackfan anemia (DBA) is an inherited pure red blood cell aplasia that often requires lifelong transfusional support. The origin of the imperfect erythrogenesis is not known. The existence of more than one molecular basis for DBA is indicated by its different modes of inheritance and widely variable clinical phenotypes. Several erythroid growth factors have been thought to have a role in the pathogenesis of DBA. However, there is neither molecular nor clinical evidence for the involvement of stem cell factor or interleukin-3. The observation of elevated erythropoietin (EPO) concentrations and an impaired in vivo and in vitro response to pharmacologic doses of recombinant human EPO has suggested a defective EPO function in the pathogenesis of DBA. We have investigated the possible involvement of the EPO receptor (EPO-R) gene in 23 patients by screening its coding sequence for mutations using single-strand conformation polymorphism (SSCP). A Southern blot and hybridization with an EPO-R probe was also performed on DNA from seven patients. No causal mutations were identified. The absence of concordant segregation of the disease with the EPO-R gene in two informative families ruled out its role in their DBA children. These findings demonstrate that DBA is not commonly associated with EPO-R gene mutations.     

Telemedicine Intervention to Improve Long-Term Risk Factor Control and Body Composition in Persons with High Cardiovascular Risk: Results from a Randomized Trial: Telehealth strategies may offer an advantage over standard institutional based interventions for improvement of cardiovascular risk in high-risk patients long-term

Background:Telehealth strategies are increasingly used to support people at high cardiovascular risk long-term, but is it unclear if these interventions are effective at improving cardiovascular risk.Objective:To evaluate the effects of a telemedicine technology-based program on risk factor control and body composition in patients at high cardiovascular risk.Methods:This is a population based randomized controlled trial. 100 patients at high and very high cardiovascular risk were randomly assigned to a telemedicine technology-based program consisting of: Comprehensive counseling on risk factors delivered by a physician; biweekly remote support via phone delivered by a trained nurse during the first three months after enrollment; and a control group receiving routine care with individual single-session counseling on patients’ current risk factors without further support. The follow-up period was 1 year.Results:Mean age of participants was 59.9 ± 4.5 years, 80% were women. Weight (–0.582; p < 0.001), waist circumference (–0.429; p = 0.01), body mass index (–0.216; p < 0.001) diastolic blood pressure (–0.881; p = 0.04), total cholesterol (–0.149; p = 0.01) and LDL cholesterol (–0.123; p = 0.003) were lower in the intervention group compared to the control group after 12-month. Body fat mass was also lower (–0.352; p = 0.01) and lean mass was higher (0.92; p = 0.03) in the intervention group. Anxiety scores (–2.5; p < 0.002) and depression scores (–2.6; p < 0.001) were also lower in the intervention group.Conclusions:Among older people at high cardiovascular risk, the addition of telehealth strategies using remote support by phone calls over a period of 3 month resulted in small but significant improvements of cardiovascular risk factors, body composition, anxiety, and depression which are maintained long-term. Such telehealth strategies may offer an advantage over standard institution-based interventions.     

传统及改良冻存法对人胎肝细胞保护效果的比较

目的:比较传统及改良冻存法对人胎肝细胞保护效果,建立一种适合于人胎肝细胞冷冻保存的方法。方法:实验于2006-07/11在广州医学院进行。①人胎肝组织取自中期终止妊娠胎儿(20周,经胎儿亲属知情同意及医院伦理委员会批准)。冷冻保护液包括A液(DMEM培养液含1.5mol/L乙二醇 体积分数为0.2胎牛血清);B液(A液 0.1mol/L蔗糖)两部分。②传统冻存法:用0.025%Ⅰ型胶原酶消化胎肝组织,分离、收集消化后的胎肝细胞。胎肝细胞在A液、B液中分别渗透平衡20min后,在冷冻保护B液中经液氮蒸汽冷平衡5min后迅速入液氮冻存,1周后复苏培养观察。③改良冻存法:胎肝组织在A液、B液中分别渗透平衡20min后,在冷冻保护液B液中经液氮蒸汽冷平衡5min后迅速入液氮冻存,1周后复苏,入DMEM培养液37℃孵育1h,胶原酶消化胎肝组织,分离、收集消化后的胎肝细胞培养观察。④新鲜分离的胎肝细胞做为对照组。⑤复苏后分离所得胎肝细胞用锥虫兰染色评估细胞活率,并记录贴壁时间,观察集落生长情况。结果:①锥虫兰染色结果显示传统冻存法复温后的胎肝细胞活率为(73.8±2.2)%,明显低于新鲜分离的胎肝细胞活率(89.2±3.6)%及改良冻存法所得胎肝细胞活率(82.5±4.2)%(P<0.01)。②贴壁情况观察发现新鲜胎肝细胞与改良冻存法胎肝细胞于培养24h开始贴壁,而传统冻存法胎肝细胞贴壁时间滞后48h。③新鲜胎肝细胞及改良冻存法胎肝细胞于第3天出现生长集落(细胞数>10为一个集落),传统冻存法胎肝细胞在第5天出现生长集落,集落的数量低于改良冻存法及对照组(P<0.01)。结论:改良冻存法可避免冻存前损伤,提高细胞对冷冻的耐受性和冻存复温后细胞的活率。     

An alpha-globin gene initiation codon mutation in a black family with HbH disease

The molecular basis of hemoglobin H disease in a Black family of Canadian origin was investigated. Affected individuals had a combination of deletion and nondeletion alpha-thalassemia mutations on different chromosomes. Cloning and sequencing of the DNA of one member with the nondeletion form revealed a new thalassemia mutation, an A---- G substitution, in the initiation codon of the remaining alpha-globin gene of a rightward (-alpha 3.7) deletion chromosome. This mutation abolished an Ncol restriction site and therefore is detectable in genomic DNA by Southern blot analysis.