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滨蒿中利胆成分的薄层光密度法测定 总被引:3,自引:0,他引:3
本文报告了用薄层光密度法测定滨蒿中三种主要利胆成分(绿原酸、对羟基苯乙酮和6,7-二甲氧基香豆素)。将浓缩的甲醇提取液点到含羧甲基纤维素钠的硅胶G板上,先后用氯仿一乙酸乙酯—甲酸(2:2:1)和环己烷—乙酸乙酯(1:1)进行分次展开,用薄层扫描仪双波长反射法直线扫描。采用外标两点法定量。三种成分的回收率为95.8~98.4%,方法的变异系数为1.83~4.48%。 相似文献
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Miller N Saada R Markovich S Hurwitz I Susswein AJ 《Journal of neurophysiology》2011,105(4):1642-1650
An increase in L-arginine hemolymph concentration acts as a postingestion signal inhibiting Aplysia feeding. At physiological concentrations (a 10-μM increase over background), the inhibitory effect of L-arginine is too weak to block feeding in hungry animals. However, a 10-μM increase in L-arginine concentration acts along with another inhibitory stimulus, the sustained presence of food odor, to inhibit feeding after a period of access to food. A physiological concentration of L-arginine also blocked the excitatory effect of a stimulus enhancing feeding, pheromones secreted by mating conspecifics. High concentrations of L-arginine (2.5 mM) alone also inhibited ad libitum feeding. L-arginine is the substrate from which nitric oxide synthase (NOS) produces nitric oxide (NO). Both an NO donor and a 10-μM increase in L-arginine inhibited biting in response to a weak food stimulus. Treatment with NOS inhibitors initiated food-finding and biting in the absence of food, indicating that food initiates feeding against a background of tonic nitrergic inhibition. Increased feeding in response to blocking NOS is accompanied by firing of the metacerebral (MCC) neuron, a monitor of food arousal. The excitatory effect on the MCC of blocking NOS is indirect. The data suggest that L-arginine acts by amplifying NO synthesis, which acts as a background stimulus inhibiting feeding. Background modulation of neural activity and behavior by NO may also be present in other systems, but such modulation may be difficult to identify because its effects are evident only in the context of additional stimuli modulating behavior. 相似文献